Click here to switch to the map view.

The map label for this gene is dapF

Identifier: 119776381

GI number: 119776381

Start: 3843457

End: 3844284

Strand: Reverse

Name: dapF

Synonym: Sama_3249

Alternate gene names: 119776381

Gene position: 3844284-3843457 (Counterclockwise)

Preceding gene: 119776382

Following gene: 119776380

Centisome position: 89.27

GC content: 54.59

Gene sequence:

>828_bases
TTGATCCACTTCACCAAGATGCATGGTCTGGGCAACGACTTTATGGTTGTCGATGGTGTGACCCAGAACGTGTTCTTCTC
GCCGGAACAAATTCGTCGTCTGGCCGACCGCAATTTCGGTATCGGGTTTGACCAGCTGTTGTTGGTTGAGCCGCCCTACG
ATCCGGACCTGGATTTTCACTATCGCATCTTCAATGCCGATGGCAGTGAGGTGGAACAGTGTGGCAATGGTGCCCGCTGC
TTTGCCCGGTTCGTTCGCAATAAAGGGCTGACCCAAAAGCATAAAATCAAGGTTAGTACCAGTGCAGGCAAGATGACACT
GCGCCTTGAACGCGATGGTGGCGTGACTGTGAATATGGGCGTTCCCATTCTCGACCCGGCACAAATCCCCTTTAAAGCTA
AAAAATTTGAAAAAACCTATCTGCTTCAGTCGGCTCAACAAACCTTTTTATGTGGTGCTGTGTCCATGGGCAACCCCCAT
GTGGTGCTGGAAGTGGATGATATTACCCAGGCTCCGGTTGCAGACGTGGGCGCCCAGTTGACACGCCACGAACGTTTTCC
CAAAGGTGTTAACGTGGGCTTTATGCAGGTTGTGGCCCCAGACCATATCAAGCTCAGAGTCTACGAGCGGGGGGCCGCCG
AGACCCTCGCCTGTGGCAGCGGTGCCTGCGCAGCCGCCGTGGTGGGGCAGCTTCAGGGGAAATTGGGCAGCCGTGTCCGG
GTAGACTTACCCGGTGGCAGTTTGACCATCAACTGGGAAGGTGAAGGTAAGCCCCTGTGGATGACAGGCCCGGCACAACA
GGTATACGACGGACAAATCCAGTTATGA

Upstream 100 bases:

>100_bases
TCAGTCAGCTCTGGCAAGGAGAGCAACTCCTGCCGTAAACTGGGGAAAGTACCCGATTTTTGGGCGCCGGAGCACTGGCC
CGGCGCTAAGGAAGCATAGC

Downstream 100 bases:

>100_bases
CAGATCCAAAGCACAGGACGGAAGCGCCCTTTGATGAGCAGATAATTCGCGAGTATCTGATTGATAACCCTGACTTTTTT
GGTCGTTATCCAGAACTCTT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MIHFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFHYRIFNADGSEVEQCGNGARC
FARFVRNKGLTQKHKIKVSTSAGKMTLRLERDGGVTVNMGVPILDPAQIPFKAKKFEKTYLLQSAQQTFLCGAVSMGNPH
VVLEVDDITQAPVADVGAQLTRHERFPKGVNVGFMQVVAPDHIKLRVYERGAAETLACGSGACAAAVVGQLQGKLGSRVR
VDLPGGSLTINWEGEGKPLWMTGPAQQVYDGQIQL

Sequences:

>Translated_275_residues
MIHFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFHYRIFNADGSEVEQCGNGARC
FARFVRNKGLTQKHKIKVSTSAGKMTLRLERDGGVTVNMGVPILDPAQIPFKAKKFEKTYLLQSAQQTFLCGAVSMGNPH
VVLEVDDITQAPVADVGAQLTRHERFPKGVNVGFMQVVAPDHIKLRVYERGAAETLACGSGACAAAVVGQLQGKLGSRVR
VDLPGGSLTINWEGEGKPLWMTGPAQQVYDGQIQL
>Mature_275_residues
MIHFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFHYRIFNADGSEVEQCGNGARC
FARFVRNKGLTQKHKIKVSTSAGKMTLRLERDGGVTVNMGVPILDPAQIPFKAKKFEKTYLLQSAQQTFLCGAVSMGNPH
VVLEVDDITQAPVADVGAQLTRHERFPKGVNVGFMQVVAPDHIKLRVYERGAAETLACGSGACAAAVVGQLQGKLGSRVR
VDLPGGSLTINWEGEGKPLWMTGPAQQVYDGQIQL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=67.8832116788321, Blast_Score=393, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_SHEAM (A1SAP5)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_929121.1
- ProteinModelPortal:   A1SAP5
- SMR:   A1SAP5
- STRING:   A1SAP5
- GeneID:   4605496
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3249
- NMPDR:   fig|326297.7.peg.3061
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   A1SAP5
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30101; Mature: 30101

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 74-74 ACT_SITE 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFH
CEEEEEECCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEE
YRIFNADGSEVEQCGNGARCFARFVRNKGLTQKHKIKVSTSAGKMTLRLERDGGVTVNMG
EEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCEEEECC
VPILDPAQIPFKAKKFEKTYLLQSAQQTFLCGAVSMGNPHVVLEVDDITQAPVADVGAQL
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHH
TRHERFPKGVNVGFMQVVAPDHIKLRVYERGAAETLACGSGACAAAVVGQLQGKLGSRVR
HHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCEEE
VDLPGGSLTINWEGEGKPLWMTGPAQQVYDGQIQL
EECCCCEEEEEECCCCCEEEEECCHHHHCCCEECC
>Mature Secondary Structure
MIHFTKMHGLGNDFMVVDGVTQNVFFSPEQIRRLADRNFGIGFDQLLLVEPPYDPDLDFH
CEEEEEECCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEE
YRIFNADGSEVEQCGNGARCFARFVRNKGLTQKHKIKVSTSAGKMTLRLERDGGVTVNMG
EEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCEEEECC
VPILDPAQIPFKAKKFEKTYLLQSAQQTFLCGAVSMGNPHVVLEVDDITQAPVADVGAQL
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHH
TRHERFPKGVNVGFMQVVAPDHIKLRVYERGAAETLACGSGACAAAVVGQLQGKLGSRVR
HHHHCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCEEE
VDLPGGSLTINWEGEGKPLWMTGPAQQVYDGQIQL
EECCCCEEEEEECCCCCEEEEECCHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA