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The map label for this gene is mscS [H]

Identifier: 119776277

GI number: 119776277

Start: 3731796

End: 3733430

Strand: Direct

Name: mscS [H]

Synonym: Sama_3145

Alternate gene names: 119776277

Gene position: 3731796-3733430 (Clockwise)

Preceding gene: 119776269

Following gene: 119776279

Centisome position: 86.66

GC content: 54.19

Gene sequence:

>1635_bases
ATGCTTACATCTTACAGCAACAGGATGTTGAATATGCGTCTGTTAATCCCTTTCCTCTTGGGCATGAGTCTGCTGGTTCC
CACGGCAGCACTGGCCGTCAGTGATGAGTTTCGCAGTAATGTTGAGCAGCTCGAGCACACAATAGCGCAGGATCTCAAGG
CCCTGTCTCAGGCCAAAGGCGAACTGGTTGCCATCAGTGAGTATCGGATAAACCAAAAAACGGCCGAATTGAGGCAGCTT
ATCAACGCCGAAATTGAAGCCCCGAACACAGACACCGCCTATCTGCAAACGCTGGTGGAAAATCAGCTGAGTTTTATTCA
GCAGACCCAAGCCTACCTGGGCAAAGACATTCAAAGGCTTAAAAATGCACTCGGCAGCAGCAATGATGCCGAGCTACTGC
TGGCACTGTCCAACCGGGAACGGGAGCGCGACACCAACTTCAAAAAAGAACTGCAAACCCTGGACTGGGGCAGCAAGCTG
GGCATGGACACACAGCCACGCCGCACCGAGCTGAAGCAGGCGCTGGCCGAACGGGCTGATCGCCTGGCAAGCCTTGTGAG
TTACACCCAGGAACAACTCAAACAGGCAGTGGATGAAGCCCGCACCGCGGGTAAAGATGTCAGTGCCGAGCAAACCGCCA
GGGTCAGTGAGCTCAAGGCCCGGCTGGAGCAAAACAGCGTTAGCCTGAGCACAGTGGTCGGCTTGCTCGAAGAGCTGGGA
GCCGATACCACGGCGCTGAAACAGGTGCTCTTTACCGTGTCGGGTGATATTACCCAGGATGTGCTGGATATCGACCTGGT
GGGCAGTCTGCTGGGTAAGTGGCTCAGCGTCGCCGAATCGCAGGCCATAGAAAATGGTCCACGGATGCTGTTTAAGGTGT
TTATCTTCCTGCTTATTCTCTTTGTCGCCAGTCTGGTTGCCAAACTGGTAAAGAGCGTGGTGAAAAAGGCCGTCAGTAAC
TCCAAACTCAAATTCAGCAAGTTACTGCAGGATTTCTTTGTCTCTCTTTCCGCCAAGGGCGTGTACACCCTGGGGTTACT
GATTGCCCTGTCGCAACTGGGTTTTGAACTTGGGCCCCTGCTGGCCGGTTTCGGTATTGCCGGTGTGATTATCGGTTTTG
CGCTGCAGGACACGCTGTCGAACTTTGCCTCCGGCATGATGATCCTGATTTACCGCCCCTATGATGTGGGAGACCTAATC
AGCGCGGCAGGCGTCACGGGCCGTGTCAGCCATATGAGCCTGGTATCGACCACCATCAAAACATTGGATAATCAGCGCCT
TATCATTCCAAACAATAAGATTTGGGGTGATACCATCAACAACATCACCGCCGAGCACCATCGCCGGGTCGATATGACCT
TTGGTATCAGCTATGCCGATAATGTCGAGCATGCGGAAAAAATCCTGCTGGATATAGTCAACAGCCATCCCAAGGTGCTG
AAAGACCCCGGCGTGACTGTGCGCCTGCATCTCCTGGGTGAATCGTCAGTGGACTTTGTGGTGCGCCCCTGGGTGCGGCC
CGATGACTACTGGGAAGTGTATTGGGACGTGACCAAGGCGGTGAAACAGCGCTTCGACGCCGAAGGCATTACCATTCCCT
TCCCGCAGCGGGATGTGCACTTCTATCGCGCTTGA

Upstream 100 bases:

>100_bases
ACACCTCGGGGCTGCAGATTCTACCAGAGCCTGACAGGTTTGGGATAAGAGTTCCGCTCCTGACTGCACTGATTGTACGC
CCGCGCCATCAGGGGCTATG

Downstream 100 bases:

>100_bases
CCACAGCAAATCGCTGAAAACAGAAAGGCACCCGAGGGTGCCTTTCTTGTCACTCATCTGCATCCATCATCCCAGGCCGA
TTATTTCGCCCTTGTCATTG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MLTSYSNRMLNMRLLIPFLLGMSLLVPTAALAVSDEFRSNVEQLEHTIAQDLKALSQAKGELVAISEYRINQKTAELRQL
INAEIEAPNTDTAYLQTLVENQLSFIQQTQAYLGKDIQRLKNALGSSNDAELLLALSNRERERDTNFKKELQTLDWGSKL
GMDTQPRRTELKQALAERADRLASLVSYTQEQLKQAVDEARTAGKDVSAEQTARVSELKARLEQNSVSLSTVVGLLEELG
ADTTALKQVLFTVSGDITQDVLDIDLVGSLLGKWLSVAESQAIENGPRMLFKVFIFLLILFVASLVAKLVKSVVKKAVSN
SKLKFSKLLQDFFVSLSAKGVYTLGLLIALSQLGFELGPLLAGFGIAGVIIGFALQDTLSNFASGMMILIYRPYDVGDLI
SAAGVTGRVSHMSLVSTTIKTLDNQRLIIPNNKIWGDTINNITAEHHRRVDMTFGISYADNVEHAEKILLDIVNSHPKVL
KDPGVTVRLHLLGESSVDFVVRPWVRPDDYWEVYWDVTKAVKQRFDAEGITIPFPQRDVHFYRA

Sequences:

>Translated_544_residues
MLTSYSNRMLNMRLLIPFLLGMSLLVPTAALAVSDEFRSNVEQLEHTIAQDLKALSQAKGELVAISEYRINQKTAELRQL
INAEIEAPNTDTAYLQTLVENQLSFIQQTQAYLGKDIQRLKNALGSSNDAELLLALSNRERERDTNFKKELQTLDWGSKL
GMDTQPRRTELKQALAERADRLASLVSYTQEQLKQAVDEARTAGKDVSAEQTARVSELKARLEQNSVSLSTVVGLLEELG
ADTTALKQVLFTVSGDITQDVLDIDLVGSLLGKWLSVAESQAIENGPRMLFKVFIFLLILFVASLVAKLVKSVVKKAVSN
SKLKFSKLLQDFFVSLSAKGVYTLGLLIALSQLGFELGPLLAGFGIAGVIIGFALQDTLSNFASGMMILIYRPYDVGDLI
SAAGVTGRVSHMSLVSTTIKTLDNQRLIIPNNKIWGDTINNITAEHHRRVDMTFGISYADNVEHAEKILLDIVNSHPKVL
KDPGVTVRLHLLGESSVDFVVRPWVRPDDYWEVYWDVTKAVKQRFDAEGITIPFPQRDVHFYRA
>Mature_544_residues
MLTSYSNRMLNMRLLIPFLLGMSLLVPTAALAVSDEFRSNVEQLEHTIAQDLKALSQAKGELVAISEYRINQKTAELRQL
INAEIEAPNTDTAYLQTLVENQLSFIQQTQAYLGKDIQRLKNALGSSNDAELLLALSNRERERDTNFKKELQTLDWGSKL
GMDTQPRRTELKQALAERADRLASLVSYTQEQLKQAVDEARTAGKDVSAEQTARVSELKARLEQNSVSLSTVVGLLEELG
ADTTALKQVLFTVSGDITQDVLDIDLVGSLLGKWLSVAESQAIENGPRMLFKVFIFLLILFVASLVAKLVKSVVKKAVSN
SKLKFSKLLQDFFVSLSAKGVYTLGLLIALSQLGFELGPLLAGFGIAGVIIGFALQDTLSNFASGMMILIYRPYDVGDLI
SAAGVTGRVSHMSLVSTTIKTLDNQRLIIPNNKIWGDTINNITAEHHRRVDMTFGISYADNVEHAEKILLDIVNSHPKVL
KDPGVTVRLHLLGESSVDFVVRPWVRPDDYWEVYWDVTKAVKQRFDAEGITIPFPQRDVHFYRA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=247, Percent_Identity=36.4372469635627, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786670, Length=204, Percent_Identity=27.9411764705882, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI2367355, Length=265, Percent_Identity=24.9056603773585, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787591, Length=198, Percent_Identity=26.7676767676768, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 60424; Mature: 60424

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSYSNRMLNMRLLIPFLLGMSLLVPTAALAVSDEFRSNVEQLEHTIAQDLKALSQAKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELVAISEYRINQKTAELRQLINAEIEAPNTDTAYLQTLVENQLSFIQQTQAYLGKDIQRL
CEEEEHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNALGSSNDAELLLALSNRERERDTNFKKELQTLDWGSKLGMDTQPRRTELKQALAERAD
HHHHCCCCCCEEEEEECCCCHHHCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHH
RLASLVSYTQEQLKQAVDEARTAGKDVSAEQTARVSELKARLEQNSVSLSTVVGLLEELG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
ADTTALKQVLFTVSGDITQDVLDIDLVGSLLGKWLSVAESQAIENGPRMLFKVFIFLLIL
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FVASLVAKLVKSVVKKAVSNSKLKFSKLLQDFFVSLSAKGVYTLGLLIALSQLGFELGPL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHH
LAGFGIAGVIIGFALQDTLSNFASGMMILIYRPYDVGDLISAAGVTGRVSHMSLVSTTIK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHH
TLDNQRLIIPNNKIWGDTINNITAEHHRRVDMTFGISYADNVEHAEKILLDIVNSHPKVL
HCCCCEEEECCCCCCCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
KDPGVTVRLHLLGESSVDFVVRPWVRPDDYWEVYWDVTKAVKQRFDAEGITIPFPQRDVH
CCCCCEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
FYRA
CCCC
>Mature Secondary Structure
MLTSYSNRMLNMRLLIPFLLGMSLLVPTAALAVSDEFRSNVEQLEHTIAQDLKALSQAKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELVAISEYRINQKTAELRQLINAEIEAPNTDTAYLQTLVENQLSFIQQTQAYLGKDIQRL
CEEEEHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNALGSSNDAELLLALSNRERERDTNFKKELQTLDWGSKLGMDTQPRRTELKQALAERAD
HHHHCCCCCCEEEEEECCCCHHHCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHH
RLASLVSYTQEQLKQAVDEARTAGKDVSAEQTARVSELKARLEQNSVSLSTVVGLLEELG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
ADTTALKQVLFTVSGDITQDVLDIDLVGSLLGKWLSVAESQAIENGPRMLFKVFIFLLIL
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FVASLVAKLVKSVVKKAVSNSKLKFSKLLQDFFVSLSAKGVYTLGLLIALSQLGFELGPL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHH
LAGFGIAGVIIGFALQDTLSNFASGMMILIYRPYDVGDLISAAGVTGRVSHMSLVSTTIK
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHH
TLDNQRLIIPNNKIWGDTINNITAEHHRRVDMTFGISYADNVEHAEKILLDIVNSHPKVL
HCCCCEEEECCCCCCCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
KDPGVTVRLHLLGESSVDFVVRPWVRPDDYWEVYWDVTKAVKQRFDAEGITIPFPQRDVH
CCCCCEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
FYRA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]