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The map label for this gene is rpoN [H]

Identifier: 119776220

GI number: 119776220

Start: 3663507

End: 3664988

Strand: Direct

Name: rpoN [H]

Synonym: Sama_3088

Alternate gene names: 119776220

Gene position: 3663507-3664988 (Clockwise)

Preceding gene: 119776219

Following gene: 119776221

Centisome position: 85.08

GC content: 52.9

Gene sequence:

>1482_bases
ATGAAAGCGTCGCTCCAGCTAAAACTGGGACAACAACTCACAATGACGCCACAGTTGCAGCAGGCCATTCGTCTGCTTCA
ACTCTCTTCGCTTGAACTGCAACAGGAAATTCAGCAAGCCCTCGACGCCAACCCACTGTTGGAGCTGGACGACGAACTGG
GCAGCGATCAGTTTGATGCCGACGGCGGCGATAAAGAATTTGATGACCAGTTCAGCCAGGAGTCCGTCTCAAACGACACC
ACGGACAGTGCCTCGCTGGAAACCTCAGACGCCATCAATCAGCAGTCCATGCCGGACGAGCTCCCGATGGATACCACCTG
GGATGAAGTCTTTACGGCTGCCCCCGTGTCTGGCCCCGGGATCCGTGGCGAAGATGATATGCCGTTTCAGGGCGAAACCA
GCGAAGGCCTCTACGAACATCTCGAATGGCAAAAAAATCTCACCCCTTTTTCCGATACCGATCTGGCCATTGCCACTGCC
ATCATAGATGCCATCGATGACAAAGGTTACTTGACCCAAAGCCTGGAGGAAATTCTCGAGGCCGTGGGCGATGACGAAGT
GGAGCTTGACGAAGTTGAGGCCGTGCTCAAACGCATTCAACACTTCGACCCAATCGGGGTGGCTGCCCGTGATTTGGCAG
AGTGCCTGCTCATTCAACTTGGGCAGTTTCCGGCGGATACCCCCCACCTGGACAACGCCAAGCTGCTTATCCGTGACCAT
TTGGACTTGATTGCCAACCGCGACTTCCGCACCCTGATGCGAAAAACACGCCTCAAGGAAGAAGAACTCAGAGACGCAAT
CGTCCTTATTCAGGGGCTGAATCCCCGTCCCGGTGAGTCGGTGACCAGCACCAAGGACGAGTATGTTATTCCGGATGTCT
CGGTCAGTAAAAAAAATGGCCGTTGGGTTGTAGAGCTTAATCCCGATTGTATGCCTAAACTGAGTGTGAACCAGCAATAT
GCCGCCATGGCGCGCACCAGTCGCAACCAGGCTGACAGCCAGTTTATCCGCGGGCATTTGCAAGAGGCCAAGTGGTTTAT
CAAGAGCCTGGAGAGCCGCAACGACACCCTGCTGAAAGTCGCCAATTGCATAGTGCAGTTCCAGCAGGGCTTTTTTGAGT
ATGGTGAAGAAGCCATGAAACCCATGGTGCTCAACGATATCGCCGAAGCGGTAGAGATGCACGAATCCACCATCTCACGG
GTCACTACCCAAAAATACATGCATACCCCAAGGGGTATCTTTGAACTCAAGTATTTTTTCTCCAGCCACGTCGGGACCGA
AGATGGCGGAGAATGCTCATCTACTGCCATCCGCGCCTTTATCAAGAAACTGGTGGCAGCAGAAAATCAGAAGAAACCAT
TGAGCGACAGCAAAATGGCGGAACTTCTGGCAGAACAAGGAATCAACGTTGCTAGACGCACCATCGCCAAATACCGAGAG
GCGATGTTGATCCCACCGTCAAACCAACGTAAGAGTTTATAA

Upstream 100 bases:

>100_bases
AGCCCCCTTCTCATATCCGGATAATCAGGGTGGTAAATCACCGGATAACCTGATAGAAAAGCTTTCTACCTGAATAATTT
AGTGAAGGATTCGCAGTACA

Downstream 100 bases:

>100_bases
CCCACCCAAGGTCTTTGGAGGAAAGTGTCTATGCAAATCAATCTGACTGGGCATCATATCGAAATCACTGATTCACTGCG
TAACTACGTAGAGAGCAAGT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDANPLLELDDELGSDQFDADGGDKEFDDQFSQESVSNDT
TDSASLETSDAINQQSMPDELPMDTTWDEVFTAAPVSGPGIRGEDDMPFQGETSEGLYEHLEWQKNLTPFSDTDLAIATA
IIDAIDDKGYLTQSLEEILEAVGDDEVELDEVEAVLKRIQHFDPIGVAARDLAECLLIQLGQFPADTPHLDNAKLLIRDH
LDLIANRDFRTLMRKTRLKEEELRDAIVLIQGLNPRPGESVTSTKDEYVIPDVSVSKKNGRWVVELNPDCMPKLSVNQQY
AAMARTSRNQADSQFIRGHLQEAKWFIKSLESRNDTLLKVANCIVQFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISR
VTTQKYMHTPRGIFELKYFFSSHVGTEDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAELLAEQGINVARRTIAKYRE
AMLIPPSNQRKSL

Sequences:

>Translated_493_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDANPLLELDDELGSDQFDADGGDKEFDDQFSQESVSNDT
TDSASLETSDAINQQSMPDELPMDTTWDEVFTAAPVSGPGIRGEDDMPFQGETSEGLYEHLEWQKNLTPFSDTDLAIATA
IIDAIDDKGYLTQSLEEILEAVGDDEVELDEVEAVLKRIQHFDPIGVAARDLAECLLIQLGQFPADTPHLDNAKLLIRDH
LDLIANRDFRTLMRKTRLKEEELRDAIVLIQGLNPRPGESVTSTKDEYVIPDVSVSKKNGRWVVELNPDCMPKLSVNQQY
AAMARTSRNQADSQFIRGHLQEAKWFIKSLESRNDTLLKVANCIVQFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISR
VTTQKYMHTPRGIFELKYFFSSHVGTEDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAELLAEQGINVARRTIAKYRE
AMLIPPSNQRKSL
>Mature_493_residues
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDANPLLELDDELGSDQFDADGGDKEFDDQFSQESVSNDT
TDSASLETSDAINQQSMPDELPMDTTWDEVFTAAPVSGPGIRGEDDMPFQGETSEGLYEHLEWQKNLTPFSDTDLAIATA
IIDAIDDKGYLTQSLEEILEAVGDDEVELDEVEAVLKRIQHFDPIGVAARDLAECLLIQLGQFPADTPHLDNAKLLIRDH
LDLIANRDFRTLMRKTRLKEEELRDAIVLIQGLNPRPGESVTSTKDEYVIPDVSVSKKNGRWVVELNPDCMPKLSVNQQY
AAMARTSRNQADSQFIRGHLQEAKWFIKSLESRNDTLLKVANCIVQFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISR
VTTQKYMHTPRGIFELKYFFSSHVGTEDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAELLAEQGINVARRTIAKYRE
AMLIPPSNQRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor recognizes a DNA element, designated as region A upstream of the pressure-regulated operon [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=495, Percent_Identity=64.6464646464647, Blast_Score=650, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55285; Mature: 55285

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDANPLLELDDELGSDQFDA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHCCCCCCCC
DGGDKEFDDQFSQESVSNDTTDSASLETSDAINQQSMPDELPMDTTWDEVFTAAPVSGPG
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC
IRGEDDMPFQGETSEGLYEHLEWQKNLTPFSDTDLAIATAIIDAIDDKGYLTQSLEEILE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
AVGDDEVELDEVEAVLKRIQHFDPIGVAARDLAECLLIQLGQFPADTPHLDNAKLLIRDH
HHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LDLIANRDFRTLMRKTRLKEEELRDAIVLIQGLNPRPGESVTSTKDEYVIPDVSVSKKNG
HHHHHCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCEECCCCCEECCCC
RWVVELNPDCMPKLSVNQQYAAMARTSRNQADSQFIRGHLQEAKWFIKSLESRNDTLLKV
EEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ANCIVQFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFELKYFF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSHVGTEDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAELLAEQGINVARRTIAKYRE
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
AMLIPPSNQRKSL
EEECCCCCCCCCC
>Mature Secondary Structure
MKASLQLKLGQQLTMTPQLQQAIRLLQLSSLELQQEIQQALDANPLLELDDELGSDQFDA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHCCCCCCCC
DGGDKEFDDQFSQESVSNDTTDSASLETSDAINQQSMPDELPMDTTWDEVFTAAPVSGPG
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC
IRGEDDMPFQGETSEGLYEHLEWQKNLTPFSDTDLAIATAIIDAIDDKGYLTQSLEEILE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
AVGDDEVELDEVEAVLKRIQHFDPIGVAARDLAECLLIQLGQFPADTPHLDNAKLLIRDH
HHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LDLIANRDFRTLMRKTRLKEEELRDAIVLIQGLNPRPGESVTSTKDEYVIPDVSVSKKNG
HHHHHCCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCEECCCCCEECCCC
RWVVELNPDCMPKLSVNQQYAAMARTSRNQADSQFIRGHLQEAKWFIKSLESRNDTLLKV
EEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ANCIVQFQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFELKYFF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSHVGTEDGGECSSTAIRAFIKKLVAAENQKKPLSDSKMAELLAEQGINVARRTIAKYRE
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
AMLIPPSNQRKSL
EEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10760597 [H]