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The map label for this gene is ligT [H]

Identifier: 119776106

GI number: 119776106

Start: 3544997

End: 3545524

Strand: Direct

Name: ligT [H]

Synonym: Sama_2974

Alternate gene names: 119776106

Gene position: 3544997-3545524 (Clockwise)

Preceding gene: 119776102

Following gene: 119776114

Centisome position: 82.32

GC content: 54.73

Gene sequence:

>528_bases
ATGTCTGATGCACAAAGACTATTTTTAGGATTCGCACTTTCGGACCAAGAGGGCGAATCCATACTTAAGCTGCAAAAACG
CCTCGGCGCCCCCGGAAAAACCGTGGAAAAAGCCAACCTGCATATGACCCTGGCCTTTTTGGGGCAAACCAGCCAACGTC
AGTGCCAAAGACTCGAAGCTGCACTCGAAGGCATCACCATGCCAAAGTTTGAGCAGCGGCTCGACTCCCTGGTTCACTGG
CGCGGCGCCGGTGTAGTCTGTTTGTGGGGCCAGGCCACAGACTCAGGCCTGACAGACCTTTACCACGGATGTCAGTCCCT
TTGCCAAAACCTGGGGCTGCATCAGAGTGAGCACGACTTTAACCCCCATATCACCCTGTTTCGCAAAGCCAAACAATTCT
CCATCCCAGAATGGCAACCGGAGCCTGTGATACTGCGGCCCGAGCGCCTTAATCTCTACCTTTCCAACAGCACCCCTGCC
GGTGTTCGTTATGAAGTGCTGCAATCCTGGCCCCTCGGCATTACCTGA

Upstream 100 bases:

>100_bases
ATGACATATTGACGCTTTCCTTCCCGGAATAGCTGTTACTTGCATTCAACCAAGAGTATAGGCAAGGTTTGGCTACTGAC
GTAGACAAAGGTAAGCCTTT

Downstream 100 bases:

>100_bases
CACATCAGGGCCTGGCAGTCAGATTCTTGTACATAATGCAGCAATCCAGGGTAAAACCCCGGGGGCTGTGGTAGCGCGAG
CGCACCTCTTCCCCAAAGCC

Product: putative 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSDAQRLFLGFALSDQEGESILKLQKRLGAPGKTVEKANLHMTLAFLGQTSQRQCQRLEAALEGITMPKFEQRLDSLVHW
RGAGVVCLWGQATDSGLTDLYHGCQSLCQNLGLHQSEHDFNPHITLFRKAKQFSIPEWQPEPVILRPERLNLYLSNSTPA
GVRYEVLQSWPLGIT

Sequences:

>Translated_175_residues
MSDAQRLFLGFALSDQEGESILKLQKRLGAPGKTVEKANLHMTLAFLGQTSQRQCQRLEAALEGITMPKFEQRLDSLVHW
RGAGVVCLWGQATDSGLTDLYHGCQSLCQNLGLHQSEHDFNPHITLFRKAKQFSIPEWQPEPVILRPERLNLYLSNSTPA
GVRYEVLQSWPLGIT
>Mature_174_residues
SDAQRLFLGFALSDQEGESILKLQKRLGAPGKTVEKANLHMTLAFLGQTSQRQCQRLEAALEGITMPKFEQRLDSLVHWR
GAGVVCLWGQATDSGLTDLYHGCQSLCQNLGLHQSEHDFNPHITLFRKAKQFSIPEWQPEPVILRPERLNLYLSNSTPAG
VRYEVLQSWPLGIT

Specific function: Specifically ligates tRNA half-molecules containing nucleoside base modifications and shows a preference among different tRNA species. Acts in the absence of ATP to form a 2'-5' phosphodiester linkage [H]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=183, Percent_Identity=32.2404371584699, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19699; Mature: 19568

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAQRLFLGFALSDQEGESILKLQKRLGAPGKTVEKANLHMTLAFLGQTSQRQCQRLEA
CCCHHEEEEEEEECCCCCHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCHHHHHHHHH
ALEGITMPKFEQRLDSLVHWRGAGVVCLWGQATDSGLTDLYHGCQSLCQNLGLHQSEHDF
HHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
NPHITLFRKAKQFSIPEWQPEPVILRPERLNLYLSNSTPAGVRYEVLQSWPLGIT
CCCEEEEEHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDAQRLFLGFALSDQEGESILKLQKRLGAPGKTVEKANLHMTLAFLGQTSQRQCQRLEA
CCHHEEEEEEEECCCCCHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCHHHHHHHHH
ALEGITMPKFEQRLDSLVHWRGAGVVCLWGQATDSGLTDLYHGCQSLCQNLGLHQSEHDF
HHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
NPHITLFRKAKQFSIPEWQPEPVILRPERLNLYLSNSTPAGVRYEVLQSWPLGIT
CCCEEEEEHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 2013578; 8940112 [H]