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The map label for this gene is rluB [H]

Identifier: 119776069

GI number: 119776069

Start: 3489985

End: 3490749

Strand: Direct

Name: rluB [H]

Synonym: Sama_2937

Alternate gene names: 119776069

Gene position: 3489985-3490749 (Clockwise)

Preceding gene: 119776068

Following gene: 119776072

Centisome position: 81.05

GC content: 56.47

Gene sequence:

>765_bases
TTGAGTGACTGCGCCATATCCAAAGGCACTCGCCTGTGTAAATACCTTGCCGAGGCGGGCGTGGCCTCACGGCGCGGTGC
ATCACGCCTGATAGAAACGGGCAGAGTCTGCATCGATGGGCAACAAGCCTCACACAGCGACAGAGTATTTGACTCAAACC
GGGTGACTGTGGATGGCGCGCCCCTGTGTCCAGCTCAGGCAAAAGCGTACTTCCTTTACCACAAACCCGTTGGCATCGAC
TGCCGCCTGCGCGCAGAGGATCCCCACAGCCTAATCCATTGTTTGCCCGGTGATTTGCGGCTATTCCCCGCAGGCCGACT
GGACAAAGACTCCCGCGGTCTCTTGCTGCTGACCAATGATGGCGAGCTGACACAGCGCCTGATGCACCCGGACTTTCACC
ATCAAAAAGGTTATCTCATTACCCTGAATAAAGCGCCCCAGCCCGATGACATTGCGGCGATTGCGGCGGGTTTTGACTAC
GGAGAAGGCCCTACCCGCCCCTGCGTAGTGGAAGCGGCTATCGACTGCGGCTATGCGACAGAAGACGGCAACCCTCGAAA
GCTAAGGATGAGGCTGACAGAGGGCAAGAAGCGCCAGATCCGTCGTCTGTGGCGGGCACGGGGCTACCGGGTGCTGGACT
TACTGAGAGAGTCGATTCAACAACTTAACATTGGCAAACTTGAACAAGGGGAAATCAGGAGTTTAACGGCCTCTGAACTT
CGCCAGCTTAAACGTACACTGGACTTGCCCAATGAACAGGATTAA

Upstream 100 bases:

>100_bases
TGTGGATACACACCTCAGCCACCATGCTGGCCGGAGTCGGCCTGGCCCTGATGGCCCGCTCCCTCATCCATCAGGCGCTG
GAAGAGCGCCGTCTCAAACC

Downstream 100 bases:

>100_bases
CCTGCAAATCACTTGACCCAAATCATCAACTGTCGCTCTACACACCTTTATTCAAAGCAATATTCATCACGGGGAGTTCG
GGTGCGAACACTGCTACGCC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSDCAISKGTRLCKYLAEAGVASRRGASRLIETGRVCIDGQQASHSDRVFDSNRVTVDGAPLCPAQAKAYFLYHKPVGID
CRLRAEDPHSLIHCLPGDLRLFPAGRLDKDSRGLLLLTNDGELTQRLMHPDFHHQKGYLITLNKAPQPDDIAAIAAGFDY
GEGPTRPCVVEAAIDCGYATEDGNPRKLRMRLTEGKKRQIRRLWRARGYRVLDLLRESIQQLNIGKLEQGEIRSLTASEL
RQLKRTLDLPNEQD

Sequences:

>Translated_254_residues
MSDCAISKGTRLCKYLAEAGVASRRGASRLIETGRVCIDGQQASHSDRVFDSNRVTVDGAPLCPAQAKAYFLYHKPVGID
CRLRAEDPHSLIHCLPGDLRLFPAGRLDKDSRGLLLLTNDGELTQRLMHPDFHHQKGYLITLNKAPQPDDIAAIAAGFDY
GEGPTRPCVVEAAIDCGYATEDGNPRKLRMRLTEGKKRQIRRLWRARGYRVLDLLRESIQQLNIGKLEQGEIRSLTASEL
RQLKRTLDLPNEQD
>Mature_253_residues
SDCAISKGTRLCKYLAEAGVASRRGASRLIETGRVCIDGQQASHSDRVFDSNRVTVDGAPLCPAQAKAYFLYHKPVGIDC
RLRAEDPHSLIHCLPGDLRLFPAGRLDKDSRGLLLLTNDGELTQRLMHPDFHHQKGYLITLNKAPQPDDIAAIAAGFDYG
EGPTRPCVVEAAIDCGYATEDGNPRKLRMRLTEGKKRQIRRLWRARGYRVLDLLRESIQQLNIGKLEQGEIRSLTASELR
QLKRTLDLPNEQD

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=34.0248962655602, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=32.4894514767932, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787524, Length=252, Percent_Identity=30.952380952381, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87081838, Length=202, Percent_Identity=29.2079207920792, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28385; Mature: 28254

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDCAISKGTRLCKYLAEAGVASRRGASRLIETGRVCIDGQQASHSDRVFDSNRVTVDGA
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEECCCEEEECCC
PLCPAQAKAYFLYHKPVGIDCRLRAEDPHSLIHCLPGDLRLFPAGRLDKDSRGLLLLTND
CCCCCCCCEEEEEECCCCCEEEEECCCHHHHHEECCCCEEEECCCCCCCCCCCEEEEECC
GELTQRLMHPDFHHQKGYLITLNKAPQPDDIAAIAAGFDYGEGPTRPCVVEAAIDCGYAT
CHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCC
EDGNPRKLRMRLTEGKKRQIRRLWRARGYRVLDLLRESIQQLNIGKLEQGEIRSLTASEL
CCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RQLKRTLDLPNEQD
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SDCAISKGTRLCKYLAEAGVASRRGASRLIETGRVCIDGQQASHSDRVFDSNRVTVDGA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEECCCEEEECCC
PLCPAQAKAYFLYHKPVGIDCRLRAEDPHSLIHCLPGDLRLFPAGRLDKDSRGLLLLTND
CCCCCCCCEEEEEECCCCCEEEEECCCHHHHHEECCCCEEEECCCCCCCCCCCEEEEECC
GELTQRLMHPDFHHQKGYLITLNKAPQPDDIAAIAAGFDYGEGPTRPCVVEAAIDCGYAT
CHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCC
EDGNPRKLRMRLTEGKKRQIRRLWRARGYRVLDLLRESIQQLNIGKLEQGEIRSLTASEL
CCCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RQLKRTLDLPNEQD
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]