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The map label for this gene is nfnB [H]

Identifier: 119776064

GI number: 119776064

Start: 3485204

End: 3485857

Strand: Direct

Name: nfnB [H]

Synonym: Sama_2932

Alternate gene names: 119776064

Gene position: 3485204-3485857 (Clockwise)

Preceding gene: 119776060

Following gene: 119776065

Centisome position: 80.94

GC content: 54.28

Gene sequence:

>654_bases
ATGCAGGAATTAAGTTTGCTCGTGCAAAAGCGTCACACCAGCAAAGCGTTTGACCCATCCCGGACAATCAGCCCGGAGCA
GATTGCCGAACTCAAAACCCTGTTGCAGTTTGCCCCTTCGTCCACCAACTCGCAGCCATGGCACTTTGTGCTGGCCTCCA
CTGCCGAAGGCAAGGCGACCATAGCCAAGTCCACCGAGCAGTTTGCCTTCAACACTCCCAAAATCCTTAATGCTTCCCAT
GTGGTTGTGCTTTGTACCAAGACCCAGATGGATGAAGATCACCTGCGCAAAGTGTTGGCTCAGGAAAAGGCCGATGGCCG
TTTCGCCAGCGATGAAGCCATGCAAAATCAACATAATGGCCGCTCATTCTTCGTCAATATGCACAGGTACGAGCTCAAAG
ACGCCCAGCACTGGATGGAAAAGCAAGTGTATCTGGCGTTGGGCACCCTGCTTTTGGGCGCGGCAGCACTGGAAATCGAT
GCCTGTCCAATCGAGGGCTTTGATGCCACCGCCCTGAATAAGGAGCTTGGCCTCAGAGAGCAAGGGCTGTGCGCCTCTGT
CATTGTGGCGTTGGGCTACCGCTCAGGTGATGATTTTAACGCGGCACTGCCCAAGTCACGCCTGCCCCAGGCTGAGATCT
TTACTGAGATCTGA

Upstream 100 bases:

>100_bases
AATTATTTTTGAAACTATAGTCAGCCAAAGCCAATTGTTTTTGCGGTGTGCAACAGTAGAATGCCAGCCATATCCCCCAG
CATTTTTCGCGGAGAATCCC

Downstream 100 bases:

>100_bases
CATCAAGCGCTGCTGCCTGTCGCCCCGGCATATTCCTGTCCTGTCGGGGCAACTTCTTTGTTCATAATTAATTCGCATTT
GTCATCCCGTCACCCTGTGG

Product: dihydropteridine reductase

Products: NA

Alternate protein names: Dihydropteridine reductase; FMN-dependent nitroreductase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MQELSLLVQKRHTSKAFDPSRTISPEQIAELKTLLQFAPSSTNSQPWHFVLASTAEGKATIAKSTEQFAFNTPKILNASH
VVVLCTKTQMDEDHLRKVLAQEKADGRFASDEAMQNQHNGRSFFVNMHRYELKDAQHWMEKQVYLALGTLLLGAAALEID
ACPIEGFDATALNKELGLREQGLCASVIVALGYRSGDDFNAALPKSRLPQAEIFTEI

Sequences:

>Translated_217_residues
MQELSLLVQKRHTSKAFDPSRTISPEQIAELKTLLQFAPSSTNSQPWHFVLASTAEGKATIAKSTEQFAFNTPKILNASH
VVVLCTKTQMDEDHLRKVLAQEKADGRFASDEAMQNQHNGRSFFVNMHRYELKDAQHWMEKQVYLALGTLLLGAAALEID
ACPIEGFDATALNKELGLREQGLCASVIVALGYRSGDDFNAALPKSRLPQAEIFTEI
>Mature_217_residues
MQELSLLVQKRHTSKAFDPSRTISPEQIAELKTLLQFAPSSTNSQPWHFVLASTAEGKATIAKSTEQFAFNTPKILNASH
VVVLCTKTQMDEDHLRKVLAQEKADGRFASDEAMQNQHNGRSFFVNMHRYELKDAQHWMEKQVYLALGTLLLGAAALEID
ACPIEGFDATALNKELGLREQGLCASVIVALGYRSGDDFNAALPKSRLPQAEIFTEI

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=209, Percent_Identity=57.4162679425837, Blast_Score=253, Evalue=6e-69,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.34 [H]

Molecular weight: Translated: 24075; Mature: 24075

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQELSLLVQKRHTSKAFDPSRTISPEQIAELKTLLQFAPSSTNSQPWHFVLASTAEGKAT
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCH
IAKSTEQFAFNTPKILNASHVVVLCTKTQMDEDHLRKVLAQEKADGRFASDEAMQNQHNG
HHHCHHHHCCCCCCEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
RSFFVNMHRYELKDAQHWMEKQVYLALGTLLLGAAALEIDACPIEGFDATALNKELGLRE
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHCCCC
QGLCASVIVALGYRSGDDFNAALPKSRLPQAEIFTEI
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MQELSLLVQKRHTSKAFDPSRTISPEQIAELKTLLQFAPSSTNSQPWHFVLASTAEGKAT
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCH
IAKSTEQFAFNTPKILNASHVVVLCTKTQMDEDHLRKVLAQEKADGRFASDEAMQNQHNG
HHHCHHHHCCCCCCEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
RSFFVNMHRYELKDAQHWMEKQVYLALGTLLLGAAALEIDACPIEGFDATALNKELGLRE
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHCCCC
QGLCASVIVALGYRSGDDFNAALPKSRLPQAEIFTEI
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7813889; 8905232; 9278503; 1472094; 9298646; 3060113; 11020276 [H]