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The map label for this gene is rstB [H]

Identifier: 119775933

GI number: 119775933

Start: 3340414

End: 3341676

Strand: Direct

Name: rstB [H]

Synonym: Sama_2801

Alternate gene names: 119775933

Gene position: 3340414-3341676 (Clockwise)

Preceding gene: 119775932

Following gene: 119775935

Centisome position: 77.57

GC content: 56.53

Gene sequence:

>1263_bases
GTGAAACGCCTCTTCGTCAGCCTTTATCTGCTGCTGAGTCTGTCCATTCTGGGCATTGGCTGGACTCTGGATAGCCTCTG
GTACCACAATGTGGACGACTCGGCCGCAGAAGACGCCCCCTTCCTGGCGCTGGCCAGTCTGTTGTCTGAAATGCCCGAAG
CCGAGCGGGCAAAACACCTCGCCAAATTCGAAGAAGACAAGACGCTGCCACTGTCACTGCTGAGTGCCAGCCAGGTAGCC
CTGTCCAATAACGAATCCCTGAGCAGCGGCAGAGTGTTTACCATTGCCCTCGATGACGATAAAGAAATGCGTCTGGTGCG
GGTGGGTGAACAGGTGCTGATGGCCGGGCCCATGGAAATTGATCCCAGAGAAAACCTGCGCGGGCTCTTTACGCTGTTTT
TCTATGGATTGCTCGCCGGTGTTGCCCTGTTGTGGGTGTGGCCGTTGTCGCGGGATATCCGCATACTGCGGGAAGCCACC
GAAGCCTTTGGCCGGGCGAAATGGGATACCCGCATCGCCCTGACCAAGCGTTCTCAGGTGAAAAGCCTGGCCGATACCTT
CAATGAGATGGCGCGCCACATCAGCGCCCTGATTGAAAACCAGCAGCACCTGACCAATGCCGTATCCCACGAAATCCGCA
CCCCGCTGGCCCGGCTCAAGTTTGCCCTGGCGCTGCTGCCCGGTTATTGCGGTAAAGACAATGACGATGAGAAACTCAAC
CAGTTCCTTGCAGATATGCAGTTGGATATCAAGGAAATGGAACAGCTGCTCTCTGAGCTCCTGACCTATGCCAGCCTGGA
AGCTCCCAGGGAGGGGCTGAAATTTGAAACCAGTAACCTCAGTGCCCTCACACGCCAGGTGACGCAACGGCTGCAAAGTT
TAAGTCAGGTGCCCATCGAACTGCATTTGCCGGAAAAGGACGTGCAAGTACCGGCAGACCCATCCCTGATTGAGCGCGCC
CTGCAGAATCTCATCACCAATGCCATCCGGTTTGCCCATACCCGCATTCGCATCGAACTGGTACGGGCCGACAATGAATG
GCGCCTGTCGGTAGAGGACGATGGCGAAGGGATCCCGCTGGTGGATCAGGGCAAGATTTTCGAGCCCTTTTATCGGGTAA
GCAGCAGCCAGAACGGCAACAAGGGACACGGGTTGGGATTGGCCATCATCAAGCGCATTATGCAGCGCCATAAAGGCAAG
GTAAGCCTTGAGAGCGAACCGGGAAAAACCCGCTTTACACTGCATATGCGGGCGCGCCGTTAG

Upstream 100 bases:

>100_bases
GCGTAAAAAGCTCGACGACAACCTGGAAACCCCGTTCCGGATTAAGACCGTCTGGGGTCAGGGATACCTGTTTGCACCCG
AGGCCTGGGCAAGCTAAGCC

Downstream 100 bases:

>100_bases
TACTCGTGGATATTGTTAAGCGAGGCAATCAGCAAACGCCGAAGATTCAATCCAAACTGAAGTTGCCAAAGTCCAGTTCG
GTATCATTTTTATCGTTTGC

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKRLFVSLYLLLSLSILGIGWTLDSLWYHNVDDSAAEDAPFLALASLLSEMPEAERAKHLAKFEEDKTLPLSLLSASQVA
LSNNESLSSGRVFTIALDDDKEMRLVRVGEQVLMAGPMEIDPRENLRGLFTLFFYGLLAGVALLWVWPLSRDIRILREAT
EAFGRAKWDTRIALTKRSQVKSLADTFNEMARHISALIENQQHLTNAVSHEIRTPLARLKFALALLPGYCGKDNDDEKLN
QFLADMQLDIKEMEQLLSELLTYASLEAPREGLKFETSNLSALTRQVTQRLQSLSQVPIELHLPEKDVQVPADPSLIERA
LQNLITNAIRFAHTRIRIELVRADNEWRLSVEDDGEGIPLVDQGKIFEPFYRVSSSQNGNKGHGLGLAIIKRIMQRHKGK
VSLESEPGKTRFTLHMRARR

Sequences:

>Translated_420_residues
MKRLFVSLYLLLSLSILGIGWTLDSLWYHNVDDSAAEDAPFLALASLLSEMPEAERAKHLAKFEEDKTLPLSLLSASQVA
LSNNESLSSGRVFTIALDDDKEMRLVRVGEQVLMAGPMEIDPRENLRGLFTLFFYGLLAGVALLWVWPLSRDIRILREAT
EAFGRAKWDTRIALTKRSQVKSLADTFNEMARHISALIENQQHLTNAVSHEIRTPLARLKFALALLPGYCGKDNDDEKLN
QFLADMQLDIKEMEQLLSELLTYASLEAPREGLKFETSNLSALTRQVTQRLQSLSQVPIELHLPEKDVQVPADPSLIERA
LQNLITNAIRFAHTRIRIELVRADNEWRLSVEDDGEGIPLVDQGKIFEPFYRVSSSQNGNKGHGLGLAIIKRIMQRHKGK
VSLESEPGKTRFTLHMRARR
>Mature_420_residues
MKRLFVSLYLLLSLSILGIGWTLDSLWYHNVDDSAAEDAPFLALASLLSEMPEAERAKHLAKFEEDKTLPLSLLSASQVA
LSNNESLSSGRVFTIALDDDKEMRLVRVGEQVLMAGPMEIDPRENLRGLFTLFFYGLLAGVALLWVWPLSRDIRILREAT
EAFGRAKWDTRIALTKRSQVKSLADTFNEMARHISALIENQQHLTNAVSHEIRTPLARLKFALALLPGYCGKDNDDEKLN
QFLADMQLDIKEMEQLLSELLTYASLEAPREGLKFETSNLSALTRQVTQRLQSLSQVPIELHLPEKDVQVPADPSLIERA
LQNLITNAIRFAHTRIRIELVRADNEWRLSVEDDGEGIPLVDQGKIFEPFYRVSSSQNGNKGHGLGLAIIKRIMQRHKGK
VSLESEPGKTRFTLHMRARR

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=440, Percent_Identity=29.5454545454545, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=29.4372294372294, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789808, Length=277, Percent_Identity=29.2418772563177, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786783, Length=289, Percent_Identity=27.3356401384083, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693157, Length=251, Percent_Identity=28.6852589641434, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790861, Length=281, Percent_Identity=29.8932384341637, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786600, Length=214, Percent_Identity=32.7102803738318, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790551, Length=253, Percent_Identity=28.4584980237154, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=29.4642857142857, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081816, Length=340, Percent_Identity=28.2352941176471, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=28.8288288288288, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=28.4403669724771, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=25.2788104089219, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789149, Length=342, Percent_Identity=26.0233918128655, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082128, Length=247, Percent_Identity=24.6963562753036, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790300, Length=315, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994928, Length=265, Percent_Identity=29.4339622641509, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788713, Length=239, Percent_Identity=26.3598326359833, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788279, Length=258, Percent_Identity=24.8062015503876, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47473; Mature: 47473

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFVSLYLLLSLSILGIGWTLDSLWYHNVDDSAAEDAPFLALASLLSEMPEAERAKHL
CHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
AKFEEDKTLPLSLLSASQVALSNNESLSSGRVFTIALDDDKEMRLVRVGEQVLMAGPMEI
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
DPRENLRGLFTLFFYGLLAGVALLWVWPLSRDIRILREATEAFGRAKWDTRIALTKRSQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
KSLADTFNEMARHISALIENQQHLTNAVSHEIRTPLARLKFALALLPGYCGKDNDDEKLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QFLADMQLDIKEMEQLLSELLTYASLEAPREGLKFETSNLSALTRQVTQRLQSLSQVPIE
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LHLPEKDVQVPADPSLIERALQNLITNAIRFAHTRIRIELVRADNEWRLSVEDDGEGIPL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCCC
VDQGKIFEPFYRVSSSQNGNKGHGLGLAIIKRIMQRHKGKVSLESEPGKTRFTLHMRARR
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKRLFVSLYLLLSLSILGIGWTLDSLWYHNVDDSAAEDAPFLALASLLSEMPEAERAKHL
CHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
AKFEEDKTLPLSLLSASQVALSNNESLSSGRVFTIALDDDKEMRLVRVGEQVLMAGPMEI
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
DPRENLRGLFTLFFYGLLAGVALLWVWPLSRDIRILREATEAFGRAKWDTRIALTKRSQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
KSLADTFNEMARHISALIENQQHLTNAVSHEIRTPLARLKFALALLPGYCGKDNDDEKLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QFLADMQLDIKEMEQLLSELLTYASLEAPREGLKFETSNLSALTRQVTQRLQSLSQVPIE
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LHLPEKDVQVPADPSLIERALQNLITNAIRFAHTRIRIELVRADNEWRLSVEDDGEGIPL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEECCCCCCCCC
VDQGKIFEPFYRVSSSQNGNKGHGLGLAIIKRIMQRHKGKVSLESEPGKTRFTLHMRARR
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]