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The map label for this gene is metK

Identifier: 119775892

GI number: 119775892

Start: 3301224

End: 3302375

Strand: Direct

Name: metK

Synonym: Sama_2760

Alternate gene names: 119775892

Gene position: 3301224-3302375 (Clockwise)

Preceding gene: 119775883

Following gene: 119775896

Centisome position: 76.66

GC content: 55.73

Gene sequence:

>1152_bases
ATGGCAAAACACTTGTTTACCTCTGAGTCGGTCTCAGAAGGTCATCCCGATAAAATTGCAGACCAGATTTCCGATGCTGT
GCTCGATGCAATCCTGGAGCAGGATCCCAAGGCCCGCGTAGCGTGCGAAACCTACGTGAAAACCGGTATGGTGATGGTCG
GTGGCGAAATCACTACCTCTGCCTGGGTCGATATCGAAGAACTGACCCGTAAAACCGTACGCGAAATCGGCTATGTGCAT
TCAGACATGGGCTTTGATGCCGATTCCTGCGCGGTACTGAGTGCCATCGGTAAGCAGTCTCCTGATATCAACCAGGGTGT
TGACCGTGCTGACCCACGTGAACAGGGTGCCGGTGACCAGGGCCTGATGTTCGGCTACGCCAGCAACGAAACCGACGTGC
TGATGCCAGCGCCTATCACCTACGCCCACGCGCTGGTGAAGCGCCAGTCAGAAGTCCGTAAAGACGGCACCCTGTCCTGG
TTGCGCCCAGACGCCAAGTCTCAGGTCACCTTCGCCTACGATGAAGGCAAGATTGTGGGTATTGACGCCATCGTACTGTC
TACCCAGCACTGCGATTCTGTCTCTCAGTCTGATCTGGTAGAAGGCGTGATGGAAACCATCATCAAGCCTGTGGTACCTG
CCCAGTGGCTGAACAAGGACACCAAGTTCTTTATCAACCCAACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGT
GGTCTGACTGGCCGCAAGATCATCGTTGACACCTACGGCGGTATGGCCCGTCACGGTGGTGGCGCCTTCTCCGGTAAAGA
CCCATCCAAGGTTGACCGCTCTGCTGCCTACGCCGCCCGTTATGTTGCCAAAAACATCGTGGCCGCTGGCCTGGCTGACC
GCTGTGAAATCCAGGTGTCCTACGCCATCGGTGTAGCCGAGCCAACCTCTATCAGCATCGAGACCTTCGGTACCGGTAAA
CTGCCAGAAGATAAGCTCATTGCCTTGGTACGTCAGCACTTCGACCTGCGCCCATATGGCCTGACCGAAATGCTGAACCT
GGCACGTCCTATCTATCAGGCCACTGCCGCTTACGGTCACTTTGGTCGTAACGAGTTCCCTTGGGAACAAACCAACAAGG
CCGAAGCACTGCGCGCAGATTCAGGTCTGTAA

Upstream 100 bases:

>100_bases
GATCCGGGGGATGATCGTGAACGTAATTTCAACTAAAATGGCCGTCTAGATGTAGATGCTTCTACACGTTTAACGCTTAA
AAAGTCGAGATTTTCTAAAC

Downstream 100 bases:

>100_bases
TCAGGCGCTTGCCCAATAAAAAACCGCCGAAAGGCGGTTTTTTTGTCTCTGGCTCACCTCAAGGTACCTCACACCGACAG
GCCTTTGCCTGTAGCAATCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEELTRKTVREIGYVH
SDMGFDADSCAVLSAIGKQSPDINQGVDRADPREQGAGDQGLMFGYASNETDVLMPAPITYAHALVKRQSEVRKDGTLSW
LRPDAKSQVTFAYDEGKIVGIDAIVLSTQHCDSVSQSDLVEGVMETIIKPVVPAQWLNKDTKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTGK
LPEDKLIALVRQHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEQTNKAEALRADSGL

Sequences:

>Translated_383_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEELTRKTVREIGYVH
SDMGFDADSCAVLSAIGKQSPDINQGVDRADPREQGAGDQGLMFGYASNETDVLMPAPITYAHALVKRQSEVRKDGTLSW
LRPDAKSQVTFAYDEGKIVGIDAIVLSTQHCDSVSQSDLVEGVMETIIKPVVPAQWLNKDTKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTGK
LPEDKLIALVRQHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEQTNKAEALRADSGL
>Mature_382_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEELTRKTVREIGYVHS
DMGFDADSCAVLSAIGKQSPDINQGVDRADPREQGAGDQGLMFGYASNETDVLMPAPITYAHALVKRQSEVRKDGTLSWL
RPDAKSQVTFAYDEGKIVGIDAIVLSTQHCDSVSQSDLVEGVMETIIKPVVPAQWLNKDTKFFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTGKL
PEDKLIALVRQHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEQTNKAEALRADSGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=375, Percent_Identity=57.8666666666667, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI4557737, Length=375, Percent_Identity=58.1333333333333, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=85.1174934725849, Blast_Score=687, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=373, Percent_Identity=56.5683646112601, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17538494, Length=373, Percent_Identity=56.3002680965147, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=55.5555555555556, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17551082, Length=374, Percent_Identity=54.8128342245989, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32565905, Length=373, Percent_Identity=50.6702412868633, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=53.0674846625767, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=52.0588235294118, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=52.8428093645485, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=65.0602409638554, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=73.9130434782609, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=56.6489361702128, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323209, Length=371, Percent_Identity=56.6037735849057, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=54.7368421052632, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=54.7368421052632, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=54.7368421052632, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=54.7368421052632, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=54.7368421052632, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=54.7368421052632, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=379, Percent_Identity=53.8258575197889, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=379, Percent_Identity=53.8258575197889, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=51.5873015873016, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=56.6666666666667, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_SHEAM (A1S9A6)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928632.1
- ProteinModelPortal:   A1S9A6
- SMR:   A1S9A6
- STRING:   A1S9A6
- GeneID:   4605007
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2760
- NMPDR:   fig|326297.7.peg.2588
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   A1S9A6
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41438; Mature: 41307

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEECCH
AWVDIEELTRKTVREIGYVHSDMGFDADSCAVLSAIGKQSPDINQGVDRADPREQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYASNETDVLMPAPITYAHALVKRQSEVRKDGTLSWLRPDAKSQVTFAYDEGKIVG
CEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCEEEE
IDAIVLSTQHCDSVSQSDLVEGVMETIIKPVVPAQWLNKDTKFFINPTGRFVIGGPMGDC
EEEEEEECHHCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISIETFGTGKLPEDKLIALVRQHFDLRPYGLTEMLNLARPIYQATAAYGH
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
FGRNEFPWEQTNKAEALRADSGL
CCCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEECCH
AWVDIEELTRKTVREIGYVHSDMGFDADSCAVLSAIGKQSPDINQGVDRADPREQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYASNETDVLMPAPITYAHALVKRQSEVRKDGTLSWLRPDAKSQVTFAYDEGKIVG
CEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCEEEE
IDAIVLSTQHCDSVSQSDLVEGVMETIIKPVVPAQWLNKDTKFFINPTGRFVIGGPMGDC
EEEEEEECHHCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVS
CCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVAEPTSISIETFGTGKLPEDKLIALVRQHFDLRPYGLTEMLNLARPIYQATAAYGH
EEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
FGRNEFPWEQTNKAEALRADSGL
CCCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA