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The map label for this gene is aprN [H]

Identifier: 119775547

GI number: 119775547

Start: 2899885

End: 2901465

Strand: Reverse

Name: aprN [H]

Synonym: Sama_2413

Alternate gene names: 119775547

Gene position: 2901465-2899885 (Counterclockwise)

Preceding gene: 119775549

Following gene: 119775546

Centisome position: 67.38

GC content: 56.93

Gene sequence:

>1581_bases
ATGCATTCAACCAAGTTGTCTTTGTCTTCACTGCTGGTTTGCGTATTGTCTGGCATAAGTCTGGGCGTAACCGCTGCGGC
GTCGAATGAAGAGTCCCGCGTTATTGTGAAGTTTAAATCTGGGATGGGACCCCAAGCCAAAGCCAGTATCCGTGCGGCGG
GAGGCACTTTGATGTTGGACCTCTCACGCCACGATGCCGCCGCTTTTTTACTGCCTGCCAGCGCTGTACAAGGGCTGTCG
CATCACCCTCTTATCGAGTATCTCGAAGCCGATGTGCTGCGTTACCCCCTGTCACAATCCGAGCCTTATGGCATTGCCAT
GGTACAGGCCAATTTGTTGTTGCCTGGGGTTAATAGTGCAGCCAACCGCACTGTGTGCATCATAGACTCGGGCATCGACA
TGGGGCATGAAGATCTCCCCAACAATCGACTCACCGGCACCTATGATGCTGGAACCGGTGATTGGGGCAGCGATGAAAAT
CACCACGGCACCCATGTGGCGGGCACCATAGCCGCGGTGAATAATGACCTTGGGGTGATAGGGGTGCATTCAGGCAGTGC
CCTGAATCTGCATATCATTAAAGTCTTTGACGCCAATGGCTGGGCGTACTCTTCAAGTTTGGTGGCCGCACTCGATAAGT
GCATCGACGCCGGTGCCAATGTCATCAATATGAGTTTGGGCGGTCCCCTTAAAAGCCGCACCGAAGACAGGGCATTCGCA
GACGCCGAGACCCTGGGAGTACTCAGCGTGGCAGCTGCCGGAAACGACGGCAACACGCGGCATTCCTATCCGGCTTCCTA
CAATGCCGTGGTTTCGGTTGCGGCCCTCGATGAAAACAAGCAGGTTGCGGATTTTTCCCAGCGTACCAGTCAGGTGGAGT
TGTCGGCGCCGGGCGTAGGCGTGCTTTCTACCCTGCCCATGGGGCAGGCAGAAGTGGCCAGTGTGTCTGCCGGTGCCAGC
GATTTTGAAGCCAACGCCATGGAGGGCAGCCCCTATGGAAGCGAGACTGCCATGCTTGCCGACTGCGGGTTTGGCGACAA
TATCTGCAACGCATCAGGGCAGCTTTGCCTGATCAGTCGCGGGAATATCAGCTTTGCCGACAAGGTACAGAACTGCGCCG
CAGGCGGTGGTGTTGGCGCCATCATCTATAACAATGTCGCAGGCGATCTCTATGGGACCCTTGGAGGCGTTAACACCACA
ATTCCTTCTGTTGGGGTGTCGGATACCACAGGTGCTGCCTTGCAGCAGACCACGGGTCAGGTTGCCACCCTCAATGTGGC
ACAGGGAAACTATGCCAGGTTCAGTGGCACCTCCATGGCAACGCCTCACGTTGCCGGTGTCGCAGCGCTGGTTTGGGGGC
TGCACACCAATTGCAGCAACACAGAAATCCGCTCGGCGTTAAGGGCTACAGCAGAAGACTTGGGGCCAACAGGACGGGAT
GACGCATACGGGTATGGTTTGGTTCGCGCTTTGGCGGCACATGACTACCTGATTGCCCACCCGTGCACCGGTGGCCAGGG
AACTGGCGGAGGCTCGGGCACCGGTGGCAGCTGCAAGGGGAAAAACAAGCAATGTAACTGA

Upstream 100 bases:

>100_bases
TGCCTATAAGTGAAGTTATAGCCTGTGTGAAAGTGTGGACGTTGAGGGTGGTTAAGGGAATGACACGGGCAGGGCATCAA
CAGATTCACGGGAGCAGGGC

Downstream 100 bases:

>100_bases
TAGCCATCTTGCTGCATCAGTCTGCCCTGCGGGTTGCAACAGCACCATGGCTACTCCTTCTCCACATTGCGCGTGGCCCT
TTGGCCACGCGTTTTTTATT

Product: cold-active alkaline serine protease

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MHSTKLSLSSLLVCVLSGISLGVTAAASNEESRVIVKFKSGMGPQAKASIRAAGGTLMLDLSRHDAAAFLLPASAVQGLS
HHPLIEYLEADVLRYPLSQSEPYGIAMVQANLLLPGVNSAANRTVCIIDSGIDMGHEDLPNNRLTGTYDAGTGDWGSDEN
HHGTHVAGTIAAVNNDLGVIGVHSGSALNLHIIKVFDANGWAYSSSLVAALDKCIDAGANVINMSLGGPLKSRTEDRAFA
DAETLGVLSVAAAGNDGNTRHSYPASYNAVVSVAALDENKQVADFSQRTSQVELSAPGVGVLSTLPMGQAEVASVSAGAS
DFEANAMEGSPYGSETAMLADCGFGDNICNASGQLCLISRGNISFADKVQNCAAGGGVGAIIYNNVAGDLYGTLGGVNTT
IPSVGVSDTTGAALQQTTGQVATLNVAQGNYARFSGTSMATPHVAGVAALVWGLHTNCSNTEIRSALRATAEDLGPTGRD
DAYGYGLVRALAAHDYLIAHPCTGGQGTGGGSGTGGSCKGKNKQCN

Sequences:

>Translated_526_residues
MHSTKLSLSSLLVCVLSGISLGVTAAASNEESRVIVKFKSGMGPQAKASIRAAGGTLMLDLSRHDAAAFLLPASAVQGLS
HHPLIEYLEADVLRYPLSQSEPYGIAMVQANLLLPGVNSAANRTVCIIDSGIDMGHEDLPNNRLTGTYDAGTGDWGSDEN
HHGTHVAGTIAAVNNDLGVIGVHSGSALNLHIIKVFDANGWAYSSSLVAALDKCIDAGANVINMSLGGPLKSRTEDRAFA
DAETLGVLSVAAAGNDGNTRHSYPASYNAVVSVAALDENKQVADFSQRTSQVELSAPGVGVLSTLPMGQAEVASVSAGAS
DFEANAMEGSPYGSETAMLADCGFGDNICNASGQLCLISRGNISFADKVQNCAAGGGVGAIIYNNVAGDLYGTLGGVNTT
IPSVGVSDTTGAALQQTTGQVATLNVAQGNYARFSGTSMATPHVAGVAALVWGLHTNCSNTEIRSALRATAEDLGPTGRD
DAYGYGLVRALAAHDYLIAHPCTGGQGTGGGSGTGGSCKGKNKQCN
>Mature_526_residues
MHSTKLSLSSLLVCVLSGISLGVTAAASNEESRVIVKFKSGMGPQAKASIRAAGGTLMLDLSRHDAAAFLLPASAVQGLS
HHPLIEYLEADVLRYPLSQSEPYGIAMVQANLLLPGVNSAANRTVCIIDSGIDMGHEDLPNNRLTGTYDAGTGDWGSDEN
HHGTHVAGTIAAVNNDLGVIGVHSGSALNLHIIKVFDANGWAYSSSLVAALDKCIDAGANVINMSLGGPLKSRTEDRAFA
DAETLGVLSVAAAGNDGNTRHSYPASYNAVVSVAALDENKQVADFSQRTSQVELSAPGVGVLSTLPMGQAEVASVSAGAS
DFEANAMEGSPYGSETAMLADCGFGDNICNASGQLCLISRGNISFADKVQNCAAGGGVGAIIYNNVAGDLYGTLGGVNTT
IPSVGVSDTTGAALQQTTGQVATLNVAQGNYARFSGTSMATPHVAGVAALVWGLHTNCSNTEIRSALRATAEDLGPTGRD
DAYGYGLVRALAAHDYLIAHPCTGGQGTGGGSGTGGSCKGKNKQCN

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320775, Length=257, Percent_Identity=28.0155642023346, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 53486; Mature: 53486

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSTKLSLSSLLVCVLSGISLGVTAAASNEESRVIVKFKSGMGPQAKASIRAAGGTLMLD
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEEECCCEEEEE
LSRHDAAAFLLPASAVQGLSHHPLIEYLEADVLRYPLSQSEPYGIAMVQANLLLPGVNSA
ECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCC
ANRTVCIIDSGIDMGHEDLPNNRLTGTYDAGTGDWGSDENHHGTHVAGTIAAVNNDLGVI
CCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCEEE
GVHSGSALNLHIIKVFDANGWAYSSSLVAALDKCIDAGANVINMSLGGPLKSRTEDRAFA
EECCCCEEEEEEEEEEECCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
DAETLGVLSVAAAGNDGNTRHSYPASYNAVVSVAALDENKQVADFSQRTSQVELSAPGVG
CHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCEEEECCCCCC
VLSTLPMGQAEVASVSAGASDFEANAMEGSPYGSETAMLADCGFGDNICNASGQLCLISR
HHHCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEC
GNISFADKVQNCAAGGGVGAIIYNNVAGDLYGTLGGVNTTIPSVGVSDTTGAALQQTTGQ
CCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCC
VATLNVAQGNYARFSGTSMATPHVAGVAALVWGLHTNCSNTEIRSALRATAEDLGPTGRD
EEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
DAYGYGLVRALAAHDYLIAHPCTGGQGTGGGSGTGGSCKGKNKQCN
CCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHSTKLSLSSLLVCVLSGISLGVTAAASNEESRVIVKFKSGMGPQAKASIRAAGGTLMLD
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEEECCCEEEEE
LSRHDAAAFLLPASAVQGLSHHPLIEYLEADVLRYPLSQSEPYGIAMVQANLLLPGVNSA
ECCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCC
ANRTVCIIDSGIDMGHEDLPNNRLTGTYDAGTGDWGSDENHHGTHVAGTIAAVNNDLGVI
CCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCEEE
GVHSGSALNLHIIKVFDANGWAYSSSLVAALDKCIDAGANVINMSLGGPLKSRTEDRAFA
EECCCCEEEEEEEEEEECCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
DAETLGVLSVAAAGNDGNTRHSYPASYNAVVSVAALDENKQVADFSQRTSQVELSAPGVG
CHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCEEEECCCCCC
VLSTLPMGQAEVASVSAGASDFEANAMEGSPYGSETAMLADCGFGDNICNASGQLCLISR
HHHCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEC
GNISFADKVQNCAAGGGVGAIIYNNVAGDLYGTLGGVNTTIPSVGVSDTTGAALQQTTGQ
CCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCC
VATLNVAQGNYARFSGTSMATPHVAGVAALVWGLHTNCSNTEIRSALRATAEDLGPTGRD
EEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
DAYGYGLVRALAAHDYLIAHPCTGGQGTGGGSGTGGSCKGKNKQCN
CCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]