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The map label for this gene is hisS

Identifier: 119775498

GI number: 119775498

Start: 2840656

End: 2841930

Strand: Reverse

Name: hisS

Synonym: Sama_2364

Alternate gene names: 119775498

Gene position: 2841930-2840656 (Counterclockwise)

Preceding gene: 119775499

Following gene: 119775497

Centisome position: 66.0

GC content: 55.61

Gene sequence:

>1275_bases
GTGGCAAAACAGATCCAAGCGATTCGCGGAATGAACGACATTCTGCCAAACCAGTCTCCCGTGTGGCAAAAGCTCGAAGC
AGTGCTGCGCGATGCCGTTGCCGCCTTCGGTTACAGCGAAATCCGCACCCCCATCGTGGAAAGTACCGATCTCTTTAAGC
GCTCCATCGGCGAAGTCACTGACATCGTTGAGAAAGAAATGTATACCTTCGAGGACAGAAACGGCGACAGCCTGACCCTG
CGCCCCGAAGGCACGGCTTCAACCGTGCGTGCCGGTAACGAGCACGGTTTGCTCTACAACCAGGAGCAACGTCTGTGGTA
CACAGGCCCCATGTTCCGTCACGAGCGTCCTCAGAAGGGACGCTATCGCCAGTTCCACCAGTTTGGTGTTGAGGTGTATG
GCATTGCCAGTGCCGATATCGATGCCGAAGTACTGATGCTGTCCAATATGCTGTGGCAAAAGCTGGGGCTGACCGAGCAT
GTGGTACTGGAAATCAACACCCTGGGTGACAGTGACGAGCGTGCAGCCTATCGCGACGCTTTGATAGCCTTCCTGGAAGT
ACACAAAGACAAGCTCGACGAAGACAGTCAGCGTCGTATGTACAGCAATCCGCTGCGGGTATTGGATACCAAAAATCCCG
AAATCCAGGCCATTCTCGCTGATGCGCCGGCTTTGATGGACTACCTCGGTGAGGAGTCAAAGTCACATTTTTCTACCCTG
CGTGAACTCTTGGACGCTGTTGGCATCCAATACCGGGTTAATCCCCGTTTGGTTCGCGGGCTGGATTATTACAACCGGAC
TGTGTTTGAATGGGTCACCGATAGCCTGGGTGCCCAGGGTACAGTGCTCGCCGGTGGTCGTTACGATGGTCTTGTGGGCC
AGTTGGGCGGTAAAGATACCCCTGCCGTGGGCTTTGCCATGGGGCTTGAGCGCATTGTGCTCTTGCTGGAAACCCTGGAG
CTTGCCAAGGATGTACCGGCCGCAGTGGATGTGTATGTTACTGCCATGGGCGACAGCTGTGTACCGGCGGCCTTTGCCAT
TGCCAACGAGCTTCGCGTGGCGCTGCCCGGGGTGCGCATCATGACCCACTGCGGTGGTGGTAACTTCAAGAAACAAATCA
AACGCGCCGACAAGAGCGGCGCAGCCCTGGCCCTTATCATTGGTGATAATGAGCTGGCCGAGGGTAAGGTGGCCATCAAG
CCGCTTCGCAATGACAACGAACAACAGCTGGTGGCTCGTGAGGCGCTGGCAGAAACAATTAAAGCATTGATTTAA

Upstream 100 bases:

>100_bases
CTAACCGCTTTGCCCGGCTCGAACGGGAAGGGCATCTCGGTTGGCATTAGGTCAACATATTGAATTTCTGAAATATTAGC
TATCCAACGAGTCGATTACA

Downstream 100 bases:

>100_bases
TTCCCACATCGGAATTGATTACAAGGGGAAGTGCGCGTGGAAATCTATAGCACAGAAGAACAACAGGTAGAGGCCATCAA
GAGCTTCTGGAAAGAATACG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKQIQAIRGMNDILPNQSPVWQKLEAVLRDAVAAFGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYTGPMFRHERPQKGRYRQFHQFGVEVYGIASADIDAEVLMLSNMLWQKLGLTEH
VVLEINTLGDSDERAAYRDALIAFLEVHKDKLDEDSQRRMYSNPLRVLDTKNPEIQAILADAPALMDYLGEESKSHFSTL
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTDSLGAQGTVLAGGRYDGLVGQLGGKDTPAVGFAMGLERIVLLLETLE
LAKDVPAAVDVYVTAMGDSCVPAAFAIANELRVALPGVRIMTHCGGGNFKKQIKRADKSGAALALIIGDNELAEGKVAIK
PLRNDNEQQLVAREALAETIKALI

Sequences:

>Translated_424_residues
MAKQIQAIRGMNDILPNQSPVWQKLEAVLRDAVAAFGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTASTVRAGNEHGLLYNQEQRLWYTGPMFRHERPQKGRYRQFHQFGVEVYGIASADIDAEVLMLSNMLWQKLGLTEH
VVLEINTLGDSDERAAYRDALIAFLEVHKDKLDEDSQRRMYSNPLRVLDTKNPEIQAILADAPALMDYLGEESKSHFSTL
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTDSLGAQGTVLAGGRYDGLVGQLGGKDTPAVGFAMGLERIVLLLETLE
LAKDVPAAVDVYVTAMGDSCVPAAFAIANELRVALPGVRIMTHCGGGNFKKQIKRADKSGAALALIIGDNELAEGKVAIK
PLRNDNEQQLVAREALAETIKALI
>Mature_423_residues
AKQIQAIRGMNDILPNQSPVWQKLEAVLRDAVAAFGYSEIRTPIVESTDLFKRSIGEVTDIVEKEMYTFEDRNGDSLTLR
PEGTASTVRAGNEHGLLYNQEQRLWYTGPMFRHERPQKGRYRQFHQFGVEVYGIASADIDAEVLMLSNMLWQKLGLTEHV
VLEINTLGDSDERAAYRDALIAFLEVHKDKLDEDSQRRMYSNPLRVLDTKNPEIQAILADAPALMDYLGEESKSHFSTLR
ELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTDSLGAQGTVLAGGRYDGLVGQLGGKDTPAVGFAMGLERIVLLLETLEL
AKDVPAAVDVYVTAMGDSCVPAAFAIANELRVALPGVRIMTHCGGGNFKKQIKRADKSGAALALIIGDNELAEGKVAIKP
LRNDNEQQLVAREALAETIKALI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=468, Percent_Identity=26.7094017094017, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI15029520, Length=472, Percent_Identity=24.5762711864407, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=65.0943396226415, Blast_Score=585, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI71993686, Length=472, Percent_Identity=25, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71993693, Length=472, Percent_Identity=25, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325290, Length=468, Percent_Identity=24.7863247863248, Blast_Score=90, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_SHEAM (A1S862)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928238.1
- ProteinModelPortal:   A1S862
- SMR:   A1S862
- STRING:   A1S862
- GeneID:   4604613
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2364
- NMPDR:   fig|326297.7.peg.2214
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   A1S862
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47036; Mature: 46905

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIQAIRGMNDILPNQSPVWQKLEAVLRDAVAAFGYSEIRTPIVESTDLFKRSIGEVT
CCCHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYTGPMFRHERPQKG
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCCCCCCCCCCC
RYRQFHQFGVEVYGIASADIDAEVLMLSNMLWQKLGLTEHVVLEINTLGDSDERAAYRDA
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHH
LIAFLEVHKDKLDEDSQRRMYSNPLRVLDTKNPEIQAILADAPALMDYLGEESKSHFSTL
HHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHH
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTDSLGAQGTVLAGGRYDGLVGQLGGKDT
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCCCCC
PAVGFAMGLERIVLLLETLELAKDVPAAVDVYVTAMGDSCVPAAFAIANELRVALPGVRI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
MTHCGGGNFKKQIKRADKSGAALALIIGDNELAEGKVAIKPLRNDNEQQLVAREALAETI
EEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
KALI
HHHC
>Mature Secondary Structure 
AKQIQAIRGMNDILPNQSPVWQKLEAVLRDAVAAFGYSEIRTPIVESTDLFKRSIGEVT
CCHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTASTVRAGNEHGLLYNQEQRLWYTGPMFRHERPQKG
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCCCCCEECCCCCEEEECCCCCCCCCCCC
RYRQFHQFGVEVYGIASADIDAEVLMLSNMLWQKLGLTEHVVLEINTLGDSDERAAYRDA
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHH
LIAFLEVHKDKLDEDSQRRMYSNPLRVLDTKNPEIQAILADAPALMDYLGEESKSHFSTL
HHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHH
RELLDAVGIQYRVNPRLVRGLDYYNRTVFEWVTDSLGAQGTVLAGGRYDGLVGQLGGKDT
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCCCCC
PAVGFAMGLERIVLLLETLELAKDVPAAVDVYVTAMGDSCVPAAFAIANELRVALPGVRI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
MTHCGGGNFKKQIKRADKSGAALALIIGDNELAEGKVAIKPLRNDNEQQLVAREALAETI
EEECCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
KALI
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA