Click here to switch to the map view.

The map label for this gene is napF [H]

Identifier: 119775360

GI number: 119775360

Start: 2688907

End: 2689404

Strand: Direct

Name: napF [H]

Synonym: Sama_2225

Alternate gene names: 119775360

Gene position: 2688907-2689404 (Clockwise)

Preceding gene: 119775359

Following gene: 119775361

Centisome position: 62.44

GC content: 58.84

Gene sequence:

>498_bases
ATGAAAGAGAACATTAATTTAAGTCGCCGCCGACTCTTCAGTCGCCGCAAGGACAACGCCCTGCGGCCACCCTTTGTGCG
CGATGATGTGGAGTTTACTGACCTGTGCAGCCGCTGCGGTGACTGCATAACGGTGTGCGAGACAAAAATCATCGTCAAGG
GAGAGGCAGGATTCCCCGAAGTTCGCTTCGACAACGCCGAGTGCACTTTCTGCGGGGAATGCGCCAAGGTCTGTCCCGAG
CCCATCTTTTCCCTCGATAAGTCCCCACCTTGGACTCTGGAGGCGAGTATCCAGGAGTCGTGCCTCGCTCACCGGGGCAT
TTGGTGCCAAAGCTGCAAAGATGCCTGCGATGTCAGTGCCATCCGCTTCACCCCGACTCTGGGCCGTGCGCCCACGCCGG
AATTATTGCTGGATGCTTGCACCGGCTGCGGCGCCTGTGTGGCCCCCTGCCCGGCCGATGCCATTGTGATGATAAAACCC
CAGACCACGCCTCAGTAA

Upstream 100 bases:

>100_bases
AAGCTCTCTGTGGATGAGATGGCCGCCAGCAGCTGGCGCTGGCAGTCCGGCAATCCCAACGGTTACCGATAAGATTCAGG
CCCTTCGGGGCCTTTTGCCT

Downstream 100 bases:

>100_bases
TCCCCGCCAAAATCAGGTCACGGCGATTAACCTTTCTGAGGTAACGCCTTGGTAAAAATTGGGGAAAGGGTGATCATAAC
CGGGACACGTCGATGTATCT

Product: ferredoxin-type protein NapF

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKENINLSRRRLFSRRKDNALRPPFVRDDVEFTDLCSRCGDCITVCETKIIVKGEAGFPEVRFDNAECTFCGECAKVCPE
PIFSLDKSPPWTLEASIQESCLAHRGIWCQSCKDACDVSAIRFTPTLGRAPTPELLLDACTGCGACVAPCPADAIVMIKP
QTTPQ

Sequences:

>Translated_165_residues
MKENINLSRRRLFSRRKDNALRPPFVRDDVEFTDLCSRCGDCITVCETKIIVKGEAGFPEVRFDNAECTFCGECAKVCPE
PIFSLDKSPPWTLEASIQESCLAHRGIWCQSCKDACDVSAIRFTPTLGRAPTPELLLDACTGCGACVAPCPADAIVMIKP
QTTPQ
>Mature_165_residues
MKENINLSRRRLFSRRKDNALRPPFVRDDVEFTDLCSRCGDCITVCETKIIVKGEAGFPEVRFDNAECTFCGECAKVCPE
PIFSLDKSPPWTLEASIQESCLAHRGIWCQSCKDACDVSAIRFTPTLGRAPTPELLLDACTGCGACVAPCPADAIVMIKP
QTTPQ

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=155, Percent_Identity=41.9354838709677, Blast_Score=137, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18148; Mature: 18148

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
10.9 %Cys+Met (Translated Protein)
9.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
10.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENINLSRRRLFSRRKDNALRPPFVRDDVEFTDLCSRCGDCITVCETKIIVKGEAGFPE
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCHHEEHHCEEEEEECCCCCCC
VRFDNAECTFCGECAKVCPEPIFSLDKSPPWTLEASIQESCLAHRGIWCQSCKDACDVSA
EEECCCCCEECHHHHHHCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHCCCE
IRFTPTLGRAPTPELLLDACTGCGACVAPCPADAIVMIKPQTTPQ
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKENINLSRRRLFSRRKDNALRPPFVRDDVEFTDLCSRCGDCITVCETKIIVKGEAGFPE
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCHHEEHHCEEEEEECCCCCCC
VRFDNAECTFCGECAKVCPEPIFSLDKSPPWTLEASIQESCLAHRGIWCQSCKDACDVSA
EEECCCCCEECHHHHHHCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHCCCE
IRFTPTLGRAPTPELLLDACTGCGACVAPCPADAIVMIKPQTTPQ
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]