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The map label for this gene is putP [H]

Identifier: 119775312

GI number: 119775312

Start: 2633235

End: 2634683

Strand: Direct

Name: putP [H]

Synonym: Sama_2177

Alternate gene names: 119775312

Gene position: 2633235-2634683 (Clockwise)

Preceding gene: 119775311

Following gene: 119775313

Centisome position: 61.15

GC content: 55.07

Gene sequence:

>1449_bases
ATGAGCATAGATACACCTATTTTAGTCACCTTTGTGGGCTATTTAGCCCTGATGATGGGGATAGGATTCTGGGCCTACAA
GGCCACAGACTCCGTTGATGATTACATTCTTGGTGGCCGCAAAATGGGCCCGGGCGTAACCGCTCTGAGTGTGGGCGCCT
CTGATATGTCCGGCTGGTTGTTGCTGGGTTTGCCCGGTGCTGTTTACCTTGGTGGTCTCGGTGAAGCCTGGATTGGTATT
GGCCTGGTGATTGGCGCCTGGTTGAACTGGCTCTTTGTGGCAAAGCGCCTGCGTATATACACCCAGCTTGCCGATAACGC
CCTGACTCTTCCAGACTTTTTCGAAAAACGCTTCCACGATAAAGCGGGTCTGCTGCGCTTTGTCTCTGCCCTGACCATAC
TGGTATTTTTTACCTTTTATGCCTCCTCGGGCATGGTGGGCGGCGCCATTCTGTTCGAAAAGGTATTTGGTCTGGATTAC
ACCGCCGCCCTGGTGATAGGTTCTGCTATCATCGTCGGCTACACCTTTGTGGGTGGATTTTTCGCCGTAAGCTGGACCGA
TTTTTTCCAGGGCTGTTTGATGCTGATTGCCCTGCTGATTGTTCCCATGGCCATTTTTACCAACAGCGAGAGCCACGCCA
GCATTGACACCCTCTCACCGGAAATGCTGTCCCTGATTGGCGAAGGCACCACACTCATGGGCGTCGCCTCCCTGATGGCT
TGGGGCCTGGGGTATTTTGGCCAGCCCCACATTTTGTCACGCTTTATGGCCATTGAGCGCCCGGAGCATTTGCCGGTGTC
ACGCCGCATTGCCATGAGCTGGATGATAGTGTCCCTAATGGGGGCATTGGCCACTGGCCTTGCCGGCAGCCTCTATTTTG
CCAAAGAACCGCTCGCCAATGCCGAGACCGTCTTTATTCATCTGGCTCAGGTGGCGTTCAATCCCTGGATTGCGGGCCTG
CTCATTGCCGCCATTCTGTCGGCTATTATGAGCACCATCGACTCCCAACTGCTGGTGTGTTCCAGCGTGGTGACCGAAGA
CTTCTATCGCAAGCTGCGCCCCAAGGCCGATGACAATGAACTAATGCGTGTCGGTCGTATCGGAGTGCTGGCCATCGCTA
TCATCGCCGGCGTCGTGGCATTGGATCCCCAAAGCAGTGTGCTCGGGCTGGTGAGCTATGCCTGGGCCGGATTTGGTGCA
GCCTTCGGCCCTGTGGTGCTCTTGTCGCTGTTTTGGCGTGGCTACAGCAAAACAGGAGCTCTGGCGACTATCATCACAGG
TGCAGTAACGGTAGTGGTATGGAAACAGCTCAGTGGCGGCTTCTTTGATCTGTACGAAATACTGCCCGGCTTTATTCTGG
CCACACTGGCCGGGGTGCTCATCAGCTTGGTCGCACCAGCAAATGACAGTGTCCGGGCCGAGTTTGACAGCTTCCAAAGC
AAACTCTGA

Upstream 100 bases:

>100_bases
ATATGCATCACAACTCCTGCGGCAATTGGGATCCGACCCAAACTTCTGATAGCATGCGTTGGCGAAAGTATTCCAATAAA
AAACCTATGGTTGACACAGC

Downstream 100 bases:

>100_bases
TAGCCCGTTTCAATCCACACATCCAAAAAAGCGTACACATGTACGCTTTTTTATTGGGTGAAGTGTAAGTTTTTGTATCT
CAGCGCCTTGAACCGCTCAC

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSIDTPILVTFVGYLALMMGIGFWAYKATDSVDDYILGGRKMGPGVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLVIGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHDKAGLLRFVSALTILVFFTFYASSGMVGGAILFEKVFGLDY
TAALVIGSAIIVGYTFVGGFFAVSWTDFFQGCLMLIALLIVPMAIFTNSESHASIDTLSPEMLSLIGEGTTLMGVASLMA
WGLGYFGQPHILSRFMAIERPEHLPVSRRIAMSWMIVSLMGALATGLAGSLYFAKEPLANAETVFIHLAQVAFNPWIAGL
LIAAILSAIMSTIDSQLLVCSSVVTEDFYRKLRPKADDNELMRVGRIGVLAIAIIAGVVALDPQSSVLGLVSYAWAGFGA
AFGPVVLLSLFWRGYSKTGALATIITGAVTVVVWKQLSGGFFDLYEILPGFILATLAGVLISLVAPANDSVRAEFDSFQS
KL

Sequences:

>Translated_482_residues
MSIDTPILVTFVGYLALMMGIGFWAYKATDSVDDYILGGRKMGPGVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLVIGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHDKAGLLRFVSALTILVFFTFYASSGMVGGAILFEKVFGLDY
TAALVIGSAIIVGYTFVGGFFAVSWTDFFQGCLMLIALLIVPMAIFTNSESHASIDTLSPEMLSLIGEGTTLMGVASLMA
WGLGYFGQPHILSRFMAIERPEHLPVSRRIAMSWMIVSLMGALATGLAGSLYFAKEPLANAETVFIHLAQVAFNPWIAGL
LIAAILSAIMSTIDSQLLVCSSVVTEDFYRKLRPKADDNELMRVGRIGVLAIAIIAGVVALDPQSSVLGLVSYAWAGFGA
AFGPVVLLSLFWRGYSKTGALATIITGAVTVVVWKQLSGGFFDLYEILPGFILATLAGVLISLVAPANDSVRAEFDSFQS
KL
>Mature_481_residues
SIDTPILVTFVGYLALMMGIGFWAYKATDSVDDYILGGRKMGPGVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGIG
LVIGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHDKAGLLRFVSALTILVFFTFYASSGMVGGAILFEKVFGLDYT
AALVIGSAIIVGYTFVGGFFAVSWTDFFQGCLMLIALLIVPMAIFTNSESHASIDTLSPEMLSLIGEGTTLMGVASLMAW
GLGYFGQPHILSRFMAIERPEHLPVSRRIAMSWMIVSLMGALATGLAGSLYFAKEPLANAETVFIHLAQVAFNPWIAGLL
IAAILSAIMSTIDSQLLVCSSVVTEDFYRKLRPKADDNELMRVGRIGVLAIAIIAGVVALDPQSSVLGLVSYAWAGFGAA
FGPVVLLSLFWRGYSKTGALATIITGAVTVVVWKQLSGGFFDLYEILPGFILATLAGVLISLVAPANDSVRAEFDSFQSK
L

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=483, Percent_Identity=47.2049689440994, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI4507035, Length=374, Percent_Identity=24.8663101604278, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI110835708, Length=563, Percent_Identity=23.6234458259325, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI17941285, Length=408, Percent_Identity=24.5098039215686, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI11141885, Length=449, Percent_Identity=24.7216035634744, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1787251, Length=478, Percent_Identity=54.18410041841, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI87082237, Length=456, Percent_Identity=28.9473684210526, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790503, Length=448, Percent_Identity=25, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790113, Length=421, Percent_Identity=23.5154394299287, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17539284, Length=210, Percent_Identity=28.0952380952381, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221459584, Length=347, Percent_Identity=26.5129682997118, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24645928, Length=380, Percent_Identity=29.7368421052632, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640370, Length=348, Percent_Identity=25.8620689655172, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221459586, Length=373, Percent_Identity=25.201072386059, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221459588, Length=375, Percent_Identity=25.0666666666667, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648033, Length=449, Percent_Identity=22.9398663697105, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356865, Length=449, Percent_Identity=22.9398663697105, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 51623; Mature: 51492

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDTPILVTFVGYLALMMGIGFWAYKATDSVDDYILGGRKMGPGVTALSVGASDMSGWL
CCCCCHHHHHHHHHHHHHHHCCHHHEECCCCHHHHHHCCCCCCCCCEEEECCCCCCCCEE
LLGLPGAVYLGGLGEAWIGIGLVIGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHD
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KAGLLRFVSALTILVFFTFYASSGMVGGAILFEKVFGLDYTAALVIGSAIIVGYTFVGGF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FAVSWTDFFQGCLMLIALLIVPMAIFTNSESHASIDTLSPEMLSLIGEGTTLMGVASLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHCCCHHHHHHHHHHH
WGLGYFGQPHILSRFMAIERPEHLPVSRRIAMSWMIVSLMGALATGLAGSLYFAKEPLAN
HHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
AETVFIHLAQVAFNPWIAGLLIAAILSAIMSTIDSQLLVCSSVVTEDFYRKLRPKADDNE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
LMRVGRIGVLAIAIIAGVVALDPQSSVLGLVSYAWAGFGAAFGPVVLLSLFWRGYSKTGA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LATIITGAVTVVVWKQLSGGFFDLYEILPGFILATLAGVLISLVAPANDSVRAEFDSFQS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
KL
CC
>Mature Secondary Structure 
SIDTPILVTFVGYLALMMGIGFWAYKATDSVDDYILGGRKMGPGVTALSVGASDMSGWL
CCCCHHHHHHHHHHHHHHHCCHHHEECCCCHHHHHHCCCCCCCCCEEEECCCCCCCCEE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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]