Click here to switch to the map view.

The map label for this gene is trpG [H]

Identifier: 119775265

GI number: 119775265

Start: 2575678

End: 2576277

Strand: Direct

Name: trpG [H]

Synonym: Sama_2130

Alternate gene names: 119775265

Gene position: 2575678-2576277 (Clockwise)

Preceding gene: 119775264

Following gene: 119775266

Centisome position: 59.81

GC content: 57.17

Gene sequence:

>600_bases
GTGAAACTCTATTTACTCGATAACTTCGACTCTTTTACTTACAACCTGGTCGACCAATTCCGCGCGCTGGGCTTTGAGGT
AGTGATTTACCGTAACGACGTTGACCCACAGCAGCTGGCAGCCAAGTTACTGGCCGAGCCTCAAACCGCACTGGTACTCT
CCCCTGGTCCGGGTGCTCCCCACGAGGCGGGTTGCATGATGGCACTGATTAAACTGGTGGCAGGAAAGCTGCCAATTCTC
GGGATATGCCTGGGTCATCAGGCGTTGGTTGAGCACTACGGCGGCAAGGTAGAGCGCGCGCCAAGTGTGGTACACGGCAA
GGCGAGCCCCACGGTTCATGACGGTCAGGGGATTTTCCGAGGGCTGCCCTCCCCCTTGCCGGTGGCACGCTATCACAGTT
TGGTCGCAACCAAGGTGCCTGACGCCCTTAAGGTCATTGCCACTACCGAAGGCATGCCCATGGCGGTGCTCCACGAAGAA
GACAAGGCGCTGGGTTTCCAGTTTCACCCTGAGTCGATTCTGACAACCCTTGGCAGTACCTTGCTGGTGCAAAGCCTGCA
ATACCTCACCGGCTTGGCTGCCAAAGGAGAGCTGCAATGA

Upstream 100 bases:

>100_bases
GTGCCGGTGTGGTGTATGACTCAGATCCCCAGGCAGAGGCAGACGAAACCCGTCAAAAAGCACAGGCAGTGATCTCTGCC
ATCAAACTGGGAGGTGGCCT

Downstream 100 bases:

>100_bases
GCAAGGATGTAAGACAACTCCTGGATAAAACCTACGGCGGTAACCCATTATCCCGCGAAGAAGCCAAATGCCTTTTTGCC
TCGCTGGTGAGGGGCGAGCT

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKLYLLDNFDSFTYNLVDQFRALGFEVVIYRNDVDPQQLAAKLLAEPQTALVLSPGPGAPHEAGCMMALIKLVAGKLPIL
GICLGHQALVEHYGGKVERAPSVVHGKASPTVHDGQGIFRGLPSPLPVARYHSLVATKVPDALKVIATTEGMPMAVLHEE
DKALGFQFHPESILTTLGSTLLVQSLQYLTGLAAKGELQ

Sequences:

>Translated_199_residues
MKLYLLDNFDSFTYNLVDQFRALGFEVVIYRNDVDPQQLAAKLLAEPQTALVLSPGPGAPHEAGCMMALIKLVAGKLPIL
GICLGHQALVEHYGGKVERAPSVVHGKASPTVHDGQGIFRGLPSPLPVARYHSLVATKVPDALKVIATTEGMPMAVLHEE
DKALGFQFHPESILTTLGSTLLVQSLQYLTGLAAKGELQ
>Mature_199_residues
MKLYLLDNFDSFTYNLVDQFRALGFEVVIYRNDVDPQQLAAKLLAEPQTALVLSPGPGAPHEAGCMMALIKLVAGKLPIL
GICLGHQALVEHYGGKVERAPSVVHGKASPTVHDGQGIFRGLPSPLPVARYHSLVATKVPDALKVIATTEGMPMAVLHEE
DKALGFQFHPESILTTLGSTLLVQSLQYLTGLAAKGELQ

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=186, Percent_Identity=58.0645161290323, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1789760, Length=188, Percent_Identity=47.8723404255319, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1788854, Length=190, Percent_Identity=26.8421052631579, Blast_Score=67, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322638, Length=192, Percent_Identity=42.7083333333333, Blast_Score=149, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324361, Length=219, Percent_Identity=27.8538812785388, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 21360; Mature: 21360

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYLLDNFDSFTYNLVDQFRALGFEVVIYRNDVDPQQLAAKLLAEPQTALVLSPGPGAP
CEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
HEAGCMMALIKLVAGKLPILGICLGHQALVEHYGGKVERAPSVVHGKASPTVHDGQGIFR
CHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCHHHH
GLPSPLPVARYHSLVATKVPDALKVIATTEGMPMAVLHEEDKALGFQFHPESILTTLGST
CCCCCCCHHHHHHHHHHCCCHHHEEEEECCCCCEEEEECCCCCCCEEECHHHHHHHHHHH
LLVQSLQYLTGLAAKGELQ
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLYLLDNFDSFTYNLVDQFRALGFEVVIYRNDVDPQQLAAKLLAEPQTALVLSPGPGAP
CEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
HEAGCMMALIKLVAGKLPILGICLGHQALVEHYGGKVERAPSVVHGKASPTVHDGQGIFR
CHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCHHHH
GLPSPLPVARYHSLVATKVPDALKVIATTEGMPMAVLHEEDKALGFQFHPESILTTLGST
CCCCCCCHHHHHHHHHHCCCHHHEEEEECCCCCEEEEECCCCCCCEEECHHHHHHHHHHH
LLVQSLQYLTGLAAKGELQ
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]