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The map label for this gene is mltB [H]

Identifier: 119775164

GI number: 119775164

Start: 2467794

End: 2468999

Strand: Reverse

Name: mltB [H]

Synonym: Sama_2029

Alternate gene names: 119775164

Gene position: 2468999-2467794 (Counterclockwise)

Preceding gene: 119775167

Following gene: 119775163

Centisome position: 57.34

GC content: 53.07

Gene sequence:

>1206_bases
TTGAAGTACCCATTCATCCTGGCCTCGTCACTGAGCATTCTCACTGCATTCTCAGCCGGTGCTGAGCCGCGCACCTTTGA
GCAATGCATTGCCGATATCAAAGTGGATGCCCAGAGTGCTGGCATCTCCCCCCAAAGCATTGCTGTACTCGATAGTGTCA
AACCATTACAGCGGGTGGTAGAGCTTGACCGAAGCCAACCCGAATTTACCCAGACCTTTGCCGATTACTTCAATAAAAGA
GTCACGGAACGCCGGATATCGGAAGGGCAAAAAATGCTCAAAACCCACGGTGCCCTGCTTAACAAGTTGCAAAAGGAGTA
TGGTGTACCACCCCAATATCTGCTGGCTTTTTGGGGACTTGAAACCAACTTTGGTGGCTACAAAGGTAAGATGCCGGTAC
TGGATGCGTTGGCGACTCTGGCCTGTGACGCCCGCCGAAGCCAGTATTTCACCCAGGAACTGCTGCAGGCACTGCGGCTT
GGCGATAAGTTTGGATTGTCATTTGAAGACATGGTCGGCTCCTGGGCCGGTGCCATGGGCCACACGCAGTTTATGCCAAC
AACCTATCTCACCTATGCCGTGGACGGCGATGGTGATGGCAAGGTCGATTTGTGGAACAGTACCGAGGATGCTTTAAGCT
CTGCTGCCAACTTTTTAATGCAGCTTGGTTGGCAGCGAAACGAGCGTTGGGGCAGGGAAGTTCTGCTGCCAAAACATTAT
GACTATGAAAGCCTTGGCAGGGACCACCGTAAACCGTTGGCCGAGTGGCAAACGTTAGGGTTAACCACCGTATTTGGTCA
TCCAATTCCCACCGCGCCCATGGACGCCGCCCTTTATTTACCAGCGGGGCATACAGGCCCGGCATTTTTGGGGTACAGCA
ACTTTGAGGTGATTCAGCGTTGGAACCGCTCAGAGTTCTATGCGCTTGCGGTGGGGCATCTGGCCGACCGCATTAACGGG
GCAAAGCCGCTTAAAGTTGCCCCACCTCTGCAGCCAAGAAGAACCCGGGATGGGCTCAAAGCCATGCAACAAAAGCTGAT
TGAACTTGGGTACGATATCGGCGCTGCAGACGGCATTATCGGCAGCAAAACCTCTGCTGCAATCCGTGACTTTCAACGGC
GCAACAAACTGGTGCCTGATGGTTTTGCCGACGATGCGACCCTTAATGCTCTGGGATTGGATGTCGCATCTATCAGCTCA
AAATGA

Upstream 100 bases:

>100_bases
CGGTTGCCTTGCGTCGCCCCTTGGTGCACGATGATGGCTCGCCTGAGTTCTTTTCTTTTGCCACCCGGCCTAGTCAGGCA
CGACTAAAGGAGTCGACGTC

Downstream 100 bases:

>100_bases
TTGCATCTTAAACAGCTGAAGCGTAACTTCGGGGCATTCTCATAATAAGGAGTGCCCCATGCAGTTGGAAGAGTTGAGCC
ATGGTCAGCGACAGCGACTG

Product: membrane-bound lytic transglycolase-like protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKYPFILASSLSILTAFSAGAEPRTFEQCIADIKVDAQSAGISPQSIAVLDSVKPLQRVVELDRSQPEFTQTFADYFNKR
VTERRISEGQKMLKTHGALLNKLQKEYGVPPQYLLAFWGLETNFGGYKGKMPVLDALATLACDARRSQYFTQELLQALRL
GDKFGLSFEDMVGSWAGAMGHTQFMPTTYLTYAVDGDGDGKVDLWNSTEDALSSAANFLMQLGWQRNERWGREVLLPKHY
DYESLGRDHRKPLAEWQTLGLTTVFGHPIPTAPMDAALYLPAGHTGPAFLGYSNFEVIQRWNRSEFYALAVGHLADRING
AKPLKVAPPLQPRRTRDGLKAMQQKLIELGYDIGAADGIIGSKTSAAIRDFQRRNKLVPDGFADDATLNALGLDVASISS
K

Sequences:

>Translated_401_residues
MKYPFILASSLSILTAFSAGAEPRTFEQCIADIKVDAQSAGISPQSIAVLDSVKPLQRVVELDRSQPEFTQTFADYFNKR
VTERRISEGQKMLKTHGALLNKLQKEYGVPPQYLLAFWGLETNFGGYKGKMPVLDALATLACDARRSQYFTQELLQALRL
GDKFGLSFEDMVGSWAGAMGHTQFMPTTYLTYAVDGDGDGKVDLWNSTEDALSSAANFLMQLGWQRNERWGREVLLPKHY
DYESLGRDHRKPLAEWQTLGLTTVFGHPIPTAPMDAALYLPAGHTGPAFLGYSNFEVIQRWNRSEFYALAVGHLADRING
AKPLKVAPPLQPRRTRDGLKAMQQKLIELGYDIGAADGIIGSKTSAAIRDFQRRNKLVPDGFADDATLNALGLDVASISS
K
>Mature_401_residues
MKYPFILASSLSILTAFSAGAEPRTFEQCIADIKVDAQSAGISPQSIAVLDSVKPLQRVVELDRSQPEFTQTFADYFNKR
VTERRISEGQKMLKTHGALLNKLQKEYGVPPQYLLAFWGLETNFGGYKGKMPVLDALATLACDARRSQYFTQELLQALRL
GDKFGLSFEDMVGSWAGAMGHTQFMPTTYLTYAVDGDGDGKVDLWNSTEDALSSAANFLMQLGWQRNERWGREVLLPKHY
DYESLGRDHRKPLAEWQTLGLTTVFGHPIPTAPMDAALYLPAGHTGPAFLGYSNFEVIQRWNRSEFYALAVGHLADRING
AKPLKVAPPLQPRRTRDGLKAMQQKLIELGYDIGAADGIIGSKTSAAIRDFQRRNKLVPDGFADDATLNALGLDVASISS
K

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=289, Percent_Identity=31.1418685121107, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44375; Mature: 44375

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYPFILASSLSILTAFSAGAEPRTFEQCIADIKVDAQSAGISPQSIAVLDSVKPLQRVV
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
ELDRSQPEFTQTFADYFNKRVTERRISEGQKMLKTHGALLNKLQKEYGVPPQYLLAFWGL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
ETNFGGYKGKMPVLDALATLACDARRSQYFTQELLQALRLGDKFGLSFEDMVGSWAGAMG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCC
HTQFMPTTYLTYAVDGDGDGKVDLWNSTEDALSSAANFLMQLGWQRNERWGREVLLPKHY
CCCCCCCEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHCCCEECCCCCC
DYESLGRDHRKPLAEWQTLGLTTVFGHPIPTAPMDAALYLPAGHTGPAFLGYSNFEVIQR
CHHHHCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEEECCCCCCCCEECCCCHHHHHH
WNRSEFYALAVGHLADRINGAKPLKVAPPLQPRRTRDGLKAMQQKLIELGYDIGAADGII
CCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSKTSAAIRDFQRRNKLVPDGFADDATLNALGLDVASISSK
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHCCCC
>Mature Secondary Structure
MKYPFILASSLSILTAFSAGAEPRTFEQCIADIKVDAQSAGISPQSIAVLDSVKPLQRVV
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
ELDRSQPEFTQTFADYFNKRVTERRISEGQKMLKTHGALLNKLQKEYGVPPQYLLAFWGL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
ETNFGGYKGKMPVLDALATLACDARRSQYFTQELLQALRLGDKFGLSFEDMVGSWAGAMG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCC
HTQFMPTTYLTYAVDGDGDGKVDLWNSTEDALSSAANFLMQLGWQRNERWGREVLLPKHY
CCCCCCCEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHCCCEECCCCCC
DYESLGRDHRKPLAEWQTLGLTTVFGHPIPTAPMDAALYLPAGHTGPAFLGYSNFEVIQR
CHHHHCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEEECCCCCCCCEECCCCHHHHHH
WNRSEFYALAVGHLADRINGAKPLKVAPPLQPRRTRDGLKAMQQKLIELGYDIGAADGII
CCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSKTSAAIRDFQRRNKLVPDGFADDATLNALGLDVASISSK
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]