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The map label for this gene is estA [H]

Identifier: 119775157

GI number: 119775157

Start: 2458429

End: 2459109

Strand: Reverse

Name: estA [H]

Synonym: Sama_2022

Alternate gene names: 119775157

Gene position: 2459109-2458429 (Counterclockwise)

Preceding gene: 119775158

Following gene: 119775156

Centisome position: 57.11

GC content: 53.45

Gene sequence:

>681_bases
ATGAACACAGAACAAACACCTTTGGACGCCATAGTGGTTGAACCCGCGGTACCTGCGCGTTACGCGGTAATTTGGCTCCA
TGGCCTTGGGGATTCCGGGGCTGGGTTTGCCCCCATAGTGCCTGCGCTTTCGCTGCCAAAGGATGCCGGTATTCGCTTTA
TATTCCCCCATGCGCCAGAACAGCCTGTGACCATCAACGGTGGTTATATCATGCGCGCCTGGTATGACATCAAAAGTATG
GATTTGCACGATAGGGCGGATTTGGCGGGTGTGCTTGAATCAGAGCACCAGATAATGGCGCTGATTGAGGCGCAAATGGC
AGCAGGTATCCCCAGTGAACGCATCCTGTTGGCCGGATTCAGTCAGGGTGGGGTAATGAGTCTTTTCTGTGGTTTGAGGT
TTCATCAGCCCCTTGCTGGCGTGATGGCACTGTCATGCTACTTGCCGGGTGCCGATACCTTGCCCGAGAACTGCCATCCC
GCCAATGCTTCGACCCCCATCTTGCAACACCATGGTGAGCAGGATGAGGTGGTGCCACTCTTTGCAGGCCAGATGGCCCA
AAAGGCACTCCGTGATGCGGGTTATGACGTTGAATGGCAAAGTTTCAACATGGGTCATAGCGTATTACCTGCTCAGTTGA
TGGAGATACGTCGCTGGTTATTGGCAAAACTGGTTAATTAG

Upstream 100 bases:

>100_bases
AGATGCCGGTGCCGTGAGTTGGACATTGATATTGGACGATGATGAGCAGCTGGACGCCATAGCAGCCTTTTATGGACTGG
AAAAGGATAAGAGATAAAAG

Downstream 100 bases:

>100_bases
TGTGCATTTGGAGTGACACTGATATGAAGGCGCACCTCAGGGTGCGTTTTTTATTTGAAACAGTCTTCAGTGTTCATTCG
ATGGTGCCGTAACAGGCGTC

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MNTEQTPLDAIVVEPAVPARYAVIWLHGLGDSGAGFAPIVPALSLPKDAGIRFIFPHAPEQPVTINGGYIMRAWYDIKSM
DLHDRADLAGVLESEHQIMALIEAQMAAGIPSERILLAGFSQGGVMSLFCGLRFHQPLAGVMALSCYLPGADTLPENCHP
ANASTPILQHHGEQDEVVPLFAGQMAQKALRDAGYDVEWQSFNMGHSVLPAQLMEIRRWLLAKLVN

Sequences:

>Translated_226_residues
MNTEQTPLDAIVVEPAVPARYAVIWLHGLGDSGAGFAPIVPALSLPKDAGIRFIFPHAPEQPVTINGGYIMRAWYDIKSM
DLHDRADLAGVLESEHQIMALIEAQMAAGIPSERILLAGFSQGGVMSLFCGLRFHQPLAGVMALSCYLPGADTLPENCHP
ANASTPILQHHGEQDEVVPLFAGQMAQKALRDAGYDVEWQSFNMGHSVLPAQLMEIRRWLLAKLVN
>Mature_226_residues
MNTEQTPLDAIVVEPAVPARYAVIWLHGLGDSGAGFAPIVPALSLPKDAGIRFIFPHAPEQPVTINGGYIMRAWYDIKSM
DLHDRADLAGVLESEHQIMALIEAQMAAGIPSERILLAGFSQGGVMSLFCGLRFHQPLAGVMALSCYLPGADTLPENCHP
ANASTPILQHHGEQDEVVPLFAGQMAQKALRDAGYDVEWQSFNMGHSVLPAQLMEIRRWLLAKLVN

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=223, Percent_Identity=39.4618834080717, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI9966764, Length=224, Percent_Identity=37.9464285714286, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI20270341, Length=222, Percent_Identity=31.0810810810811, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17508057, Length=216, Percent_Identity=34.7222222222222, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323147, Length=216, Percent_Identity=33.7962962962963, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45553055, Length=217, Percent_Identity=35.0230414746544, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45553057, Length=217, Percent_Identity=35.0230414746544, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21357257, Length=217, Percent_Identity=35.0230414746544, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI161078179, Length=211, Percent_Identity=29.3838862559242, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24558; Mature: 24558

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEQTPLDAIVVEPAVPARYAVIWLHGLGDSGAGFAPIVPALSLPKDAGIRFIFPHAPE
CCCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCCHHCCCCCCCCCCCEEEEECCCCC
QPVTINGGYIMRAWYDIKSMDLHDRADLAGVLESEHQIMALIEAQMAAGIPSERILLAGF
CCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEC
SQGGVMSLFCGLRFHQPLAGVMALSCYLPGADTLPENCHPANASTPILQHHGEQDEVVPL
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEHH
FAGQMAQKALRDAGYDVEWQSFNMGHSVLPAQLMEIRRWLLAKLVN
HHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTEQTPLDAIVVEPAVPARYAVIWLHGLGDSGAGFAPIVPALSLPKDAGIRFIFPHAPE
CCCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCCHHCCCCCCCCCCCEEEEECCCCC
QPVTINGGYIMRAWYDIKSMDLHDRADLAGVLESEHQIMALIEAQMAAGIPSERILLAGF
CCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEC
SQGGVMSLFCGLRFHQPLAGVMALSCYLPGADTLPENCHPANASTPILQHHGEQDEVVPL
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEHH
FAGQMAQKALRDAGYDVEWQSFNMGHSVLPAQLMEIRRWLLAKLVN
HHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA