Click here to switch to the map view.

The map label for this gene is ushA [H]

Identifier: 119775156

GI number: 119775156

Start: 2456354

End: 2458069

Strand: Reverse

Name: ushA [H]

Synonym: Sama_2021

Alternate gene names: 119775156

Gene position: 2458069-2456354 (Counterclockwise)

Preceding gene: 119775157

Following gene: 119775155

Centisome position: 57.08

GC content: 52.16

Gene sequence:

>1716_bases
ATGTCACATAAACTGATGAAAGGATTGCTTGCCACTGCGGTACTGGCTGCTCTAACCGGCTGTATGGATGCCGATCCGAA
AGAGCCGGTTGTACCAACGACCTGCGCTGAAGCCGGCGATGCCTGTAAGACCTTCACTGTGCTGCATACCAACGACAACC
ACGGTCGTTTCTGGGAAAACAAGGACGGTGAGTACGGCATGGCTGCCCGTAAGGCGCTGATTGACCAGATCCGCGGTGAA
GTGGAAGGCAATGGCGGCGAAGTGCTGCTGCTTTCCGGTGGTGACATTAATACCGGCGTACCCGAGTCCGATCTGCAGGA
TGCAGTCCCTGATTTTATCGGTATGACCAAGATTGGTTACGATGCCATGGCTGTGGGTAACCACGAGTTTGATAACCCAC
TGAATATCCTTGATATGCAACGCCAGCTGGCTGACTTCCCATTCCTGTCTGCCAACATCTACAAGGCCGATGGTACCCGC
TACTTCGATGCTTATAAGGTGTTCGACGTGAACGGTGTCCGTATCGCTGTTGTGGGTCTGACCACAGAAGACACTGCCAA
GATTGGCAATCCTGAATTTATCAGTGAACTGACCTTTACCGATCCAAAAGAAGAAGTGGCCAAAGTTATCAAGGAAATCA
AAGACGGCGATAAGGCCGATATGATTTTCGCCGTGACTCACATGGGTCACTATGCTGATGCACAAAACGGCAGCAATGCT
CCTGGCGATGTGGCCATGGCTCGCAGCCTGAACAAGGGCGATCTGCAACTGGTGATTGGTGGTCACTCTCAAAACCCAGT
GTGTATGGAGCCCGGTACCAACAATTACGCCGATTTCAAAGCGGGTGATGAATGTACCCCTGATCAGCAAAACGGTACCT
GGATCATGCAGGCTCATGAGTGGGGCAAGTATGTTGGCCGTGCCGACTTTGAATACTTCAATGGTGAACTGCACCTGGCT
GGTTACAAGCTCATCCCGGTTAACCTGAAGAAAACCGTTGAGGATGAGTCAGGCAACAAGAGTAAGGTACTGGTAGGTGA
TGCCATTACCCCGGATGCCGAGCTGAAAGAGCTGCTGCAGTACTATCAGGATAAAGGTCAGGCTTCTTTGGATGAAGTGA
TTGCTACCACAGACGATAAGCTGGAAGGCGATCGCACTATGGTACGTGGTCAGCAAACCAACCTGGGCCGTCTGCTTGCC
ATGGCACAGTCTGTAAAAGTGAATGCCGACGTGGGTGTAATGAACTCAGGCGGTGTGCGTGCCTCCATCGCGGCCGGTGA
TATCTCTTACCGTGACGTACTGACTGTACAGCCATTTGGCAACATGGTGACCTTAAGCACCATGACAGGCGCCGAGCTGA
CTGAGTACCTGGGCGCCGTTGCCTCTATGCAGCGTGGTTCAGGTGCATACGCTCAAATCACCGGTGTGAAAATGACAGTG
GATTGTACTGCCAAAACCGTGGCCATCAGCGATGTAAACGGTAAAGGTTACGATGCCGGTGCCTCTTACACCTTTACTGT
TCCAAGCTTCAACGCCGCTGGCGGTGATGGCTACCCAGTACTGGCGCCTGTTCAAACAGGTTATGTAGATGCCGACGTGC
TGTACGACTTCTTCAAGCAGAAGGGCAGCATCAAGGCCGCCGATTACAACCCTATGGGTGACATCGTTTACGAAAACTCC
AACAGCCCACTGGGTTGTGAAGTGACTGCTCAGTAA

Upstream 100 bases:

>100_bases
TTCCCCGTCAAAATTGCTATTATGTTGCGCGACTAAAGGCCGAAGTGCCTGGTCTGCATTTGATGATGGTTAAAATAAAT
AAAATCATGGGGTTGATAAT

Downstream 100 bases:

>100_bases
TCCTCAGGTAGTTTCGTTAATAAAAAGCCGGCTAAGCCGGCTTTTTGATTTTATGGCCTTGAATTCTAAATCCCCAAACC
CCAAATAGGTTTCAGAGCCG

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSHKLMKGLLATAVLAALTGCMDADPKEPVVPTTCAEAGDACKTFTVLHTNDNHGRFWENKDGEYGMAARKALIDQIRGE
VEGNGGEVLLLSGGDINTGVPESDLQDAVPDFIGMTKIGYDAMAVGNHEFDNPLNILDMQRQLADFPFLSANIYKADGTR
YFDAYKVFDVNGVRIAVVGLTTEDTAKIGNPEFISELTFTDPKEEVAKVIKEIKDGDKADMIFAVTHMGHYADAQNGSNA
PGDVAMARSLNKGDLQLVIGGHSQNPVCMEPGTNNYADFKAGDECTPDQQNGTWIMQAHEWGKYVGRADFEYFNGELHLA
GYKLIPVNLKKTVEDESGNKSKVLVGDAITPDAELKELLQYYQDKGQASLDEVIATTDDKLEGDRTMVRGQQTNLGRLLA
MAQSVKVNADVGVMNSGGVRASIAAGDISYRDVLTVQPFGNMVTLSTMTGAELTEYLGAVASMQRGSGAYAQITGVKMTV
DCTAKTVAISDVNGKGYDAGASYTFTVPSFNAAGGDGYPVLAPVQTGYVDADVLYDFFKQKGSIKAADYNPMGDIVYENS
NSPLGCEVTAQ

Sequences:

>Translated_571_residues
MSHKLMKGLLATAVLAALTGCMDADPKEPVVPTTCAEAGDACKTFTVLHTNDNHGRFWENKDGEYGMAARKALIDQIRGE
VEGNGGEVLLLSGGDINTGVPESDLQDAVPDFIGMTKIGYDAMAVGNHEFDNPLNILDMQRQLADFPFLSANIYKADGTR
YFDAYKVFDVNGVRIAVVGLTTEDTAKIGNPEFISELTFTDPKEEVAKVIKEIKDGDKADMIFAVTHMGHYADAQNGSNA
PGDVAMARSLNKGDLQLVIGGHSQNPVCMEPGTNNYADFKAGDECTPDQQNGTWIMQAHEWGKYVGRADFEYFNGELHLA
GYKLIPVNLKKTVEDESGNKSKVLVGDAITPDAELKELLQYYQDKGQASLDEVIATTDDKLEGDRTMVRGQQTNLGRLLA
MAQSVKVNADVGVMNSGGVRASIAAGDISYRDVLTVQPFGNMVTLSTMTGAELTEYLGAVASMQRGSGAYAQITGVKMTV
DCTAKTVAISDVNGKGYDAGASYTFTVPSFNAAGGDGYPVLAPVQTGYVDADVLYDFFKQKGSIKAADYNPMGDIVYENS
NSPLGCEVTAQ
>Mature_570_residues
SHKLMKGLLATAVLAALTGCMDADPKEPVVPTTCAEAGDACKTFTVLHTNDNHGRFWENKDGEYGMAARKALIDQIRGEV
EGNGGEVLLLSGGDINTGVPESDLQDAVPDFIGMTKIGYDAMAVGNHEFDNPLNILDMQRQLADFPFLSANIYKADGTRY
FDAYKVFDVNGVRIAVVGLTTEDTAKIGNPEFISELTFTDPKEEVAKVIKEIKDGDKADMIFAVTHMGHYADAQNGSNAP
GDVAMARSLNKGDLQLVIGGHSQNPVCMEPGTNNYADFKAGDECTPDQQNGTWIMQAHEWGKYVGRADFEYFNGELHLAG
YKLIPVNLKKTVEDESGNKSKVLVGDAITPDAELKELLQYYQDKGQASLDEVIATTDDKLEGDRTMVRGQQTNLGRLLAM
AQSVKVNADVGVMNSGGVRASIAAGDISYRDVLTVQPFGNMVTLSTMTGAELTEYLGAVASMQRGSGAYAQITGVKMTVD
CTAKTVAISDVNGKGYDAGASYTFTVPSFNAAGGDGYPVLAPVQTGYVDADVLYDFFKQKGSIKAADYNPMGDIVYENSN
SPLGCEVTAQ

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=524, Percent_Identity=24.8091603053435, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1786687, Length=562, Percent_Identity=50.355871886121, Blast_Score=558, Evalue=1e-160,
Organism=Drosophila melanogaster, GI19922446, Length=529, Percent_Identity=25.1417769376182, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24654424, Length=529, Percent_Identity=25.1417769376182, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922444, Length=557, Percent_Identity=25.4937163375224, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28573524, Length=533, Percent_Identity=25.515947467167, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24641187, Length=460, Percent_Identity=25.8695652173913, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI221329836, Length=456, Percent_Identity=21.4912280701754, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 61204; Mature: 61073

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKLMKGLLATAVLAALTGCMDADPKEPVVPTTCAEAGDACKTFTVLHTNDNHGRFWEN
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCC
KDGEYGMAARKALIDQIRGEVEGNGGEVLLLSGGDINTGVPESDLQDAVPDFIGMTKIGY
CCCCCCHHHHHHHHHHHCCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
DAMAVGNHEFDNPLNILDMQRQLADFPFLSANIYKADGTRYFDAYKVFDVNGVRIAVVGL
CEEEECCCCCCCCHHHHHHHHHHHCCCEEECEEEECCCCEEEEEEEEEECCCEEEEEEEE
TTEDTAKIGNPEFISELTFTDPKEEVAKVIKEIKDGDKADMIFAVTHMGHYADAQNGSNA
CCCCHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
PGDVAMARSLNKGDLQLVIGGHSQNPVCMEPGTNNYADFKAGDECTPDQQNGTWIMQAHE
CCCHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECH
WGKYVGRADFEYFNGELHLAGYKLIPVNLKKTVEDESGNKSKVLVGDAITPDAELKELLQ
HHHHCCCCCCEEECCEEEEEEEEEEEECHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHH
YYQDKGQASLDEVIATTDDKLEGDRTMVRGQQTNLGRLLAMAQSVKVNADVGVMNSGGVR
HHHCCCCCCHHHHHHCCCCCCCCCHHEECCCCCCHHHHHHHHHHEEEECCEEEECCCCEE
ASIAAGDISYRDVLTVQPFGNMVTLSTMTGAELTEYLGAVASMQRGSGAYAQITGVKMTV
EEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEE
DCTAKTVAISDVNGKGYDAGASYTFTVPSFNAAGGDGYPVLAPVQTGYVDADVLYDFFKQ
ECCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHC
KGSIKAADYNPMGDIVYENSNSPLGCEVTAQ
CCCEEECCCCCCCCEEEECCCCCCCEEEECC
>Mature Secondary Structure 
SHKLMKGLLATAVLAALTGCMDADPKEPVVPTTCAEAGDACKTFTVLHTNDNHGRFWEN
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCC
KDGEYGMAARKALIDQIRGEVEGNGGEVLLLSGGDINTGVPESDLQDAVPDFIGMTKIGY
CCCCCCHHHHHHHHHHHCCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
DAMAVGNHEFDNPLNILDMQRQLADFPFLSANIYKADGTRYFDAYKVFDVNGVRIAVVGL
CEEEECCCCCCCCHHHHHHHHHHHCCCEEECEEEECCCCEEEEEEEEEECCCEEEEEEEE
TTEDTAKIGNPEFISELTFTDPKEEVAKVIKEIKDGDKADMIFAVTHMGHYADAQNGSNA
CCCCHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
PGDVAMARSLNKGDLQLVIGGHSQNPVCMEPGTNNYADFKAGDECTPDQQNGTWIMQAHE
CCCHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECH
WGKYVGRADFEYFNGELHLAGYKLIPVNLKKTVEDESGNKSKVLVGDAITPDAELKELLQ
HHHHCCCCCCEEECCEEEEEEEEEEEECHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHH
YYQDKGQASLDEVIATTDDKLEGDRTMVRGQQTNLGRLLAMAQSVKVNADVGVMNSGGVR
HHHCCCCCCHHHHHHCCCCCCCCCHHEECCCCCCHHHHHHHHHHEEEECCEEEECCCCEE
ASIAAGDISYRDVLTVQPFGNMVTLSTMTGAELTEYLGAVASMQRGSGAYAQITGVKMTV
EEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEE
DCTAKTVAISDVNGKGYDAGASYTFTVPSFNAAGGDGYPVLAPVQTGYVDADVLYDFFKQ
ECCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHC
KGSIKAADYNPMGDIVYENSNSPLGCEVTAQ
CCCEEECCCCCCCCEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA