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The map label for this gene is garR [C]

Identifier: 119775110

GI number: 119775110

Start: 2403659

End: 2404534

Strand: Reverse

Name: garR [C]

Synonym: Sama_1975

Alternate gene names: 119775110

Gene position: 2404534-2403659 (Counterclockwise)

Preceding gene: 119775111

Following gene: 119775107

Centisome position: 55.84

GC content: 56.51

Gene sequence:

>876_bases
ATGGCAAAAGTGGCATTTCTCGGACTGGGGGTGATGGGATATCCCATGGCCGGACATTTGGTCAAGGCTGGTCACCAGGT
GACTGTGTATAACCGGACCACCGCCAAGGCTGAAGCCTGGGTGAAGCAGTTTGGTGGCGCCATGGCGCTGAGTCCAAAGC
TTGCGGCTGAAGACCAGGACATCGTTTTTACCTGCGTGGGCAACGATAAAGATTTGCACCAGGTGGTACTTGGAGAAGAT
GGCGTTATCCATGGTCTCAATGCAGGTGCCGTGCTGGTTGACCACACCACAGCGTCTGCCGACATTGCCCGCGAGATTGG
CGCCGCGCTGAACGCTCAAGGCTTTGGCTTTGTTGACGCACCAGTCTCAGGCGGTCAGGCCGGCGCCGAAAATGGCGTAC
TGACCGTGATGGCAGGCGGTGATGAAGCCGTGTTTAACCAGGTAAAAGACGTCATCATGAGCTACGCCCGCTGCGCCGAG
CGTCTGGGTGAAACAGGTGCAGGCCAGCTCACCAAGATGGTGAACCAAATTTGTATCGCCGGTGTGGTCCAAGGGCTGGC
CGAAGCACTGCATTTTGCCCGTAGCGCAGGCCTTGATGGCGAAAAAGTGGTGGAAGTCATCAGCAAAGGCGCCGCCCAAA
GCTGGCAGATGGAAAACCGCTATAAGTCCATGTGGGCCGGTGAGTACAACTTCGGGTTTGCCATAGACTGGATGCGTAAA
GACTTGGGTATCGCCCTGGAAGAAGCCCGCCGTAATGGCTCGCATCTGCCGCTGACAGCCCTTGTGGATCAGTTTTACTC
AGAAGTACAGGGCATGGGGGGCAATCGCTGGGATACCTCAAGCCTGATGGCCCGCCTCGAAAAAAGCCGAAAATAA

Upstream 100 bases:

>100_bases
GGCGCGTTACCGGCAGCTATCACAGCGGCACCAACGCCTGTACCCGGCAGTAAAATCATTATTTAAAACACGCTGATCGC
GAGACACGAAAGGAAGAACA

Downstream 100 bases:

>100_bases
CTCCTGGTTAAATCAATCGTCAGCTAGTTCGGTGGTAGAGCCGTTCCCGATAAAACAAAAAGCCCGCATGAAGCGGGCTT
TTTACTGCATTAGCTTTGGG

Product: 2-hydroxy-3-oxopropionate reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAKVAFLGLGVMGYPMAGHLVKAGHQVTVYNRTTAKAEAWVKQFGGAMALSPKLAAEDQDIVFTCVGNDKDLHQVVLGED
GVIHGLNAGAVLVDHTTASADIAREIGAALNAQGFGFVDAPVSGGQAGAENGVLTVMAGGDEAVFNQVKDVIMSYARCAE
RLGETGAGQLTKMVNQICIAGVVQGLAEALHFARSAGLDGEKVVEVISKGAAQSWQMENRYKSMWAGEYNFGFAIDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLMARLEKSRK

Sequences:

>Translated_291_residues
MAKVAFLGLGVMGYPMAGHLVKAGHQVTVYNRTTAKAEAWVKQFGGAMALSPKLAAEDQDIVFTCVGNDKDLHQVVLGED
GVIHGLNAGAVLVDHTTASADIAREIGAALNAQGFGFVDAPVSGGQAGAENGVLTVMAGGDEAVFNQVKDVIMSYARCAE
RLGETGAGQLTKMVNQICIAGVVQGLAEALHFARSAGLDGEKVVEVISKGAAQSWQMENRYKSMWAGEYNFGFAIDWMRK
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLMARLEKSRK
>Mature_290_residues
AKVAFLGLGVMGYPMAGHLVKAGHQVTVYNRTTAKAEAWVKQFGGAMALSPKLAAEDQDIVFTCVGNDKDLHQVVLGEDG
VIHGLNAGAVLVDHTTASADIAREIGAALNAQGFGFVDAPVSGGQAGAENGVLTVMAGGDEAVFNQVKDVIMSYARCAER
LGETGAGQLTKMVNQICIAGVVQGLAEALHFARSAGLDGEKVVEVISKGAAQSWQMENRYKSMWAGEYNFGFAIDWMRKD
LGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLMARLEKSRK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=26.7857142857143, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=28.2312925170068, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=37.3702422145329, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=34.3859649122807, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1790315, Length=290, Percent_Identity=30.3448275862069, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789092, Length=285, Percent_Identity=35.0877192982456, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=26.2773722627737, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17542558, Length=236, Percent_Identity=25.4237288135593, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=29.9319727891156, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=29.9319727891156, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=26.5957446808511, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30923; Mature: 30791

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFLGLGVMGYPMAGHLVKAGHQVTVYNRTTAKAEAWVKQFGGAMALSPKLAAEDQD
CCCEEEECCCHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCCCCC
IVFTCVGNDKDLHQVVLGEDGVIHGLNAGAVLVDHTTASADIAREIGAALNAQGFGFVDA
EEEEEECCCCCHHHHEECCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEC
PVSGGQAGAENGVLTVMAGGDEAVFNQVKDVIMSYARCAERLGETGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALHFARSAGLDGEKVVEVISKGAAQSWQMENRYKSMWAGEYNFGFAIDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLMARLEKSRK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKVAFLGLGVMGYPMAGHLVKAGHQVTVYNRTTAKAEAWVKQFGGAMALSPKLAAEDQD
CCEEEECCCHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEECCCCCCCCCC
IVFTCVGNDKDLHQVVLGEDGVIHGLNAGAVLVDHTTASADIAREIGAALNAQGFGFVDA
EEEEEECCCCCHHHHEECCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEC
PVSGGQAGAENGVLTVMAGGDEAVFNQVKDVIMSYARCAERLGETGAGQLTKMVNQICIA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GVVQGLAEALHFARSAGLDGEKVVEVISKGAAQSWQMENRYKSMWAGEYNFGFAIDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
DLGIALEEARRNGSHLPLTALVDQFYSEVQGMGGNRWDTSSLMARLEKSRK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA