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The map label for this gene is cspA [H]

Identifier: 119775105

GI number: 119775105

Start: 2398716

End: 2398925

Strand: Reverse

Name: cspA [H]

Synonym: Sama_1970

Alternate gene names: 119775105

Gene position: 2398925-2398716 (Counterclockwise)

Preceding gene: 119775106

Following gene: 119775100

Centisome position: 55.71

GC content: 49.05

Gene sequence:

>210_bases
ATGTCTCAAGTGACTGGTGTAGTTAAGTGGTTCAACGCTGATAAAGGCTTTGGTTTTATCGCTCAGGAAGCTGGCCCAGA
TGTGTTTGTTCACTTCCGCGCCATCAACACTGACGGTTTCAAGACTCTGGACGAAGGTCAGAAGGTGTCTTTCACCGTGA
CCCAGGGTCAGAAAGGTCCTCAGGCTGAGAACGTAACCGTAATCGGTTAA

Upstream 100 bases:

>100_bases
ACGACCAGTTTGTCCTGTAAACATTAAGTAATACCGGCTTTTCCCGCTCCACCGCCCTAACCGGCAATTGTTTAAATATT
TAATGTTACAGGATACCAAT

Downstream 100 bases:

>100_bases
TTCTCCCAAGAGATGTATAAAAGCCGTGGCAAATGCCACGGCTTTTTTGTTGCCTCGAATTTGTCAGTACCGGCTTTTTT
CATGCCATGTAACGATTTCT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CSP-A; 7.4 kDa cold shock protein; CS7.4 [H]

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MSQVTGVVKWFNADKGFGFIAQEAGPDVFVHFRAINTDGFKTLDEGQKVSFTVTQGQKGPQAENVTVIG

Sequences:

>Translated_69_residues
MSQVTGVVKWFNADKGFGFIAQEAGPDVFVHFRAINTDGFKTLDEGQKVSFTVTQGQKGPQAENVTVIG
>Mature_68_residues
SQVTGVVKWFNADKGFGFIAQEAGPDVFVHFRAINTDGFKTLDEGQKVSFTVTQGQKGPQAENVTVIG

Specific function: Binds to and stimulates the transcription of the CCAAT- containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3' [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=67.1641791044776, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=62.3188405797101, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI2367114, Length=68, Percent_Identity=58.8235294117647, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=59.7014925373134, Blast_Score=84, Evalue=3e-18,
Organism=Escherichia coli, GI1788126, Length=69, Percent_Identity=60.8695652173913, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1787834, Length=68, Percent_Identity=52.9411764705882, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=50, Blast_Score=65, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7447; Mature: 7316

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVTGVVKWFNADKGFGFIAQEAGPDVFVHFRAINTDGFKTLDEGQKVSFTVTQGQKGP
CCCCCEEEEEECCCCCCEEEECCCCCCEEEEEEEECCCCCEECCCCCEEEEEEECCCCCC
QAENVTVIG
CCCCEEEEC
>Mature Secondary Structure 
SQVTGVVKWFNADKGFGFIAQEAGPDVFVHFRAINTDGFKTLDEGQKVSFTVTQGQKGP
CCCCEEEEEECCCCCCEEEECCCCCCEEEEEEEECCCCCEECCCCCEEEEEEECCCCCC
QAENVTVIG
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]