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The map label for this gene is yeiE [H]

Identifier: 119775017

GI number: 119775017

Start: 2297333

End: 2298241

Strand: Reverse

Name: yeiE [H]

Synonym: Sama_1882

Alternate gene names: 119775017

Gene position: 2298241-2297333 (Counterclockwise)

Preceding gene: 119775018

Following gene: 119775015

Centisome position: 53.37

GC content: 55.45

Gene sequence:

>909_bases
ATGAAAATGACCCTCAGGCAGCTTGAGGTGTTCGAAGCCATTGCCCGCTCGGGGCAGGTGAGTAAAGCCGCTCAGTGGCT
TGGGATGACGGGGCCGGCAGCCTCAATGGCTCTCGCAGAGCTTGAAAAGCAACTGTCAGTGCGACTCTTTGAACGTGTTG
GCAATCGGCTGCGACTTAATGCTCAGGGCGCACAGCTGCTGCCTCTTGCCACCGAGCTGTTGGCTCAGGCATCCAAAGTT
GAAGCCCTGTTTAATGCCAGCGGCGCAGTGTTGGCCGGTGAGCTTAAAGTCGGTGCCAGTACCACTATTGGCAATTTTCT
GCTGGCCAAGGCCGCGGTGGCGTTCGGTCAGCAGCATCTGGATGCTCTGGTGGACGTGTCTATTCACAATACCTCTGCGG
TTATCGAACAGGTGGCTAACTTTGAGGTGGAGCTTGGCTTTATTGAAGGCCACTGTGTGGATCGCAGACTGGATGTCCGC
GCCTGGCATCAAGACCATTTGTGTGTTTTTTGTCATCCGGCCCACCCGTTGGCAGGGCGAAAGGTCTCGGTCGATGAGGT
CAGGCAGCAATACTGGGTGCTGAGGGAAGAGGGCTCTGGTACGCGGGAACATTTTGTCAACGCAGCCCATGCACTCGGGC
TGCATCCCGAGGCGCGGTTTTGTTTTTCAACCGCTGATGCCATCAAACTCGCGGTAAAACAGGGGGCAGGGCTTGGCGTG
TTATCTGAATTGGCACTTGAGCGGGAACTTGCCCGCCGTGAGCTCGCCCTGATTGAAGTGACCGGCCTTTCCATTACCCG
GCCATTCTTTCTTATCCGCCACAAAAGCCGGCAATCAACGGCACTTGCCAATGCGTTTGTGCAGTTTTGTCAGAACTTTT
TCAATATTCAGGAAAGGCCGGTAGTCTAG

Upstream 100 bases:

>100_bases
CGCTAACCCCACTTGCCGATAAGTGGAAAGTCGCCCAACCCAAATGGACGACCGCAGCCACTGCAGGCCGTGCCGGTCGG
GTTGTCGGAGCAAACGAGCG

Downstream 100 bases:

>100_bases
CGGCGATACGCCGCATCGCCTCTGGATGATGCCAGTGGTTGTCAGGGCTGATAGCCCTTATCAATTTGAGTCGGTTTTGC
TTCGGCCGCTTCACGGGCAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKMTLRQLEVFEAIARSGQVSKAAQWLGMTGPAASMALAELEKQLSVRLFERVGNRLRLNAQGAQLLPLATELLAQASKV
EALFNASGAVLAGELKVGASTTIGNFLLAKAAVAFGQQHLDALVDVSIHNTSAVIEQVANFEVELGFIEGHCVDRRLDVR
AWHQDHLCVFCHPAHPLAGRKVSVDEVRQQYWVLREEGSGTREHFVNAAHALGLHPEARFCFSTADAIKLAVKQGAGLGV
LSELALERELARRELALIEVTGLSITRPFFLIRHKSRQSTALANAFVQFCQNFFNIQERPVV

Sequences:

>Translated_302_residues
MKMTLRQLEVFEAIARSGQVSKAAQWLGMTGPAASMALAELEKQLSVRLFERVGNRLRLNAQGAQLLPLATELLAQASKV
EALFNASGAVLAGELKVGASTTIGNFLLAKAAVAFGQQHLDALVDVSIHNTSAVIEQVANFEVELGFIEGHCVDRRLDVR
AWHQDHLCVFCHPAHPLAGRKVSVDEVRQQYWVLREEGSGTREHFVNAAHALGLHPEARFCFSTADAIKLAVKQGAGLGV
LSELALERELARRELALIEVTGLSITRPFFLIRHKSRQSTALANAFVQFCQNFFNIQERPVV
>Mature_302_residues
MKMTLRQLEVFEAIARSGQVSKAAQWLGMTGPAASMALAELEKQLSVRLFERVGNRLRLNAQGAQLLPLATELLAQASKV
EALFNASGAVLAGELKVGASTTIGNFLLAKAAVAFGQQHLDALVDVSIHNTSAVIEQVANFEVELGFIEGHCVDRRLDVR
AWHQDHLCVFCHPAHPLAGRKVSVDEVRQQYWVLREEGSGTREHFVNAAHALGLHPEARFCFSTADAIKLAVKQGAGLGV
LSELALERELARRELALIEVTGLSITRPFFLIRHKSRQSTALANAFVQFCQNFFNIQERPVV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=34.4709897610922, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1787530, Length=312, Percent_Identity=25, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081904, Length=214, Percent_Identity=27.5700934579439, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=292, Percent_Identity=21.9178082191781, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693105, Length=184, Percent_Identity=26.6304347826087, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=297, Percent_Identity=25.2525252525253, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788748, Length=309, Percent_Identity=24.2718446601942, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33109; Mature: 33109

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTLRQLEVFEAIARSGQVSKAAQWLGMTGPAASMALAELEKQLSVRLFERVGNRLRLN
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEC
AQGAQLLPLATELLAQASKVEALFNASGAVLAGELKVGASTTIGNFLLAKAAVAFGQQHL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DALVDVSIHNTSAVIEQVANFEVELGFIEGHCVDRRLDVRAWHQDHLCVFCHPAHPLAGR
HHHHHEEECCHHHHHHHHHCCEEEEEEEECEEECCCCCCEEECCCCEEEEEECCCCCCCC
KVSVDEVRQQYWVLREEGSGTREHFVNAAHALGLHPEARFCFSTADAIKLAVKQGAGLGV
EECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHH
LSELALERELARRELALIEVTGLSITRPFFLIRHKSRQSTALANAFVQFCQNFFNIQERP
HHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
VV
CC
>Mature Secondary Structure
MKMTLRQLEVFEAIARSGQVSKAAQWLGMTGPAASMALAELEKQLSVRLFERVGNRLRLN
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEC
AQGAQLLPLATELLAQASKVEALFNASGAVLAGELKVGASTTIGNFLLAKAAVAFGQQHL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DALVDVSIHNTSAVIEQVANFEVELGFIEGHCVDRRLDVRAWHQDHLCVFCHPAHPLAGR
HHHHHEEECCHHHHHHHHHCCEEEEEEEECEEECCCCCCEEECCCCEEEEEECCCCCCCC
KVSVDEVRQQYWVLREEGSGTREHFVNAAHALGLHPEARFCFSTADAIKLAVKQGAGLGV
EECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHH
LSELALERELARRELALIEVTGLSITRPFFLIRHKSRQSTALANAFVQFCQNFFNIQERP
HHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
VV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]