Click here to switch to the map view.

The map label for this gene is pyrF [H]

Identifier: 119774875

GI number: 119774875

Start: 2129830

End: 2130531

Strand: Direct

Name: pyrF [H]

Synonym: Sama_1740

Alternate gene names: 119774875

Gene position: 2129830-2130531 (Clockwise)

Preceding gene: 119774874

Following gene: 119774876

Centisome position: 49.46

GC content: 53.56

Gene sequence:

>702_bases
ATGAGAGACAAGCCCATTCTGGTTGCCCTTGATTTTGACAACAAGTATTCAGCGTTGCAGTTGGTTGATAAGCTCGATCC
CGCCATGTGCCGCCTCAAGGTGGGCAAGGAGATGTTCACTCTGTTTGGGCCAGAGCTGGTAAAAGACATCCAAAATCGTG
GTTTCGACTTATTCCTTGACCTTAAATTTCACGATATTCCCAACACCGTGGCCAAGGCGGTTGCCGCAGCGGCTGAGCTT
GGGGTCTGGATGGTCAACGTCCACGCATCAGGTGGTTTTGCCATGATGGAAGCGGCGCGCAAAGCCTTGCTGCCATACGG
CGACAATGCGCCGCTGTTGATTGCCGTGACTGTGCTTACCTCCATGAGCGATGAAGATCTTAAGCTTTTGGGAATCGACG
TTGGTGCTGCTGAGCACGTGAAGCGTCTTGCCAAATTAACCCGTGACGCGGGCCTCGATGGTGTGGTGTGCTCGGCACAG
GAAGCCACTATGCTTAAAGAACTGTGTGGGCGCGATTTTAAACTCATTACCCCGGGTATACGCCCTGAGGGGAGTGAGGC
CGGCGATCAGCATCGCATCATGACGCCCGCTCAGGCTCTGCAAGCAGGCTCGGATTATCTGGTGATTGGTCGTCCGATCA
CCAAGTCCGCAGATCCACTCAAGACTCTGACGGAAATTCACCAGTCAATCCGCGGAATATAA

Upstream 100 bases:

>100_bases
ACTCTACTGGCATTGCCCTTCCTGTAAGTCCTGGGGCAGTATTGCCCGGGTGAAAGGATTGGACGGCGAATAACCCTAGT
CCCACTGCATCGGAGATCCC

Downstream 100 bases:

>100_bases
ACCACAGGGCCACAATAACCCGCACTCACGGCTGAAACGCAATTAGCGCTGACAAGGAGTTAAGATGTTCGACTTTAACG
GGAAAAACATTTTGGTGGTC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MRDKPILVALDFDNKYSALQLVDKLDPAMCRLKVGKEMFTLFGPELVKDIQNRGFDLFLDLKFHDIPNTVAKAVAAAAEL
GVWMVNVHASGGFAMMEAARKALLPYGDNAPLLIAVTVLTSMSDEDLKLLGIDVGAAEHVKRLAKLTRDAGLDGVVCSAQ
EATMLKELCGRDFKLITPGIRPEGSEAGDQHRIMTPAQALQAGSDYLVIGRPITKSADPLKTLTEIHQSIRGI

Sequences:

>Translated_233_residues
MRDKPILVALDFDNKYSALQLVDKLDPAMCRLKVGKEMFTLFGPELVKDIQNRGFDLFLDLKFHDIPNTVAKAVAAAAEL
GVWMVNVHASGGFAMMEAARKALLPYGDNAPLLIAVTVLTSMSDEDLKLLGIDVGAAEHVKRLAKLTRDAGLDGVVCSAQ
EATMLKELCGRDFKLITPGIRPEGSEAGDQHRIMTPAQALQAGSDYLVIGRPITKSADPLKTLTEIHQSIRGI
>Mature_233_residues
MRDKPILVALDFDNKYSALQLVDKLDPAMCRLKVGKEMFTLFGPELVKDIQNRGFDLFLDLKFHDIPNTVAKAVAAAAEL
GVWMVNVHASGGFAMMEAARKALLPYGDNAPLLIAVTVLTSMSDEDLKLLGIDVGAAEHVKRLAKLTRDAGLDGVVCSAQ
EATMLKELCGRDFKLITPGIRPEGSEAGDQHRIMTPAQALQAGSDYLVIGRPITKSADPLKTLTEIHQSIRGI

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=229, Percent_Identity=66.8122270742358, Blast_Score=312, Evalue=1e-86,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25253; Mature: 25253

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00599 AA_TRANSFER_CLASS_2 ; PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDKPILVALDFDNKYSALQLVDKLDPAMCRLKVGKEMFTLFGPELVKDIQNRGFDLFLD
CCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAKAVAAAAELGVWMVNVHASGGFAMMEAARKALLPYGDNAPLLIAVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHH
SMSDEDLKLLGIDVGAAEHVKRLAKLTRDAGLDGVVCSAQEATMLKELCGRDFKLITPGI
CCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEECCCC
RPEGSEAGDQHRIMTPAQALQAGSDYLVIGRPITKSADPLKTLTEIHQSIRGI
CCCCCCCCCCCCEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDKPILVALDFDNKYSALQLVDKLDPAMCRLKVGKEMFTLFGPELVKDIQNRGFDLFLD
CCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAKAVAAAAELGVWMVNVHASGGFAMMEAARKALLPYGDNAPLLIAVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHH
SMSDEDLKLLGIDVGAAEHVKRLAKLTRDAGLDGVVCSAQEATMLKELCGRDFKLITPGI
CCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEECCCC
RPEGSEAGDQHRIMTPAQALQAGSDYLVIGRPITKSADPLKTLTEIHQSIRGI
CCCCCCCCCCCCEECHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]