Click here to switch to the map view.

The map label for this gene is sppA [H]

Identifier: 119774858

GI number: 119774858

Start: 2107498

End: 2109351

Strand: Reverse

Name: sppA [H]

Synonym: Sama_1723

Alternate gene names: 119774858

Gene position: 2109351-2107498 (Counterclockwise)

Preceding gene: 119774861

Following gene: 119774857

Centisome position: 48.98

GC content: 52.37

Gene sequence:

>1854_bases
ATGGCCAAAAAACCCCTGACAACAAAAAACACCCTGCTTCTTATCTGGAATACGATAAATTTCGTTCGTAAGCTGATAAT
TAATATTGTATTTTTTCCAATTCTTATTCTGGTGTTGATTGTTGGTATCGTCGCTTTAAGCACAGAAGATGAGCTGCGTA
TTGAATCCGGCAGTGCCTTGGTGTTGGACCTATCCGGCAATCTGGTTGATCAGGCCAGGCGTGTCGATCCGCTAACCCAA
CTTATGCGTCAGGGCAATCAAAACAGCGAAGACACTGAAGTCCTGCTTTCAGACGTGCTTTATGTCATCGAAAACGCGAC
CCATGACGAACGCATCGGCGCTATCGTGCTCGATCTTGGCAATATGCGCGCCGGGATCAGTAAACTCACCGCTGTGGGCG
AGGCTCTGAACGGTTTTCGTGAGACAGGTAAAAAGGTCGTGGCTATCGGCGATTGGTACAGCCGCAGTCAGTATTTCCTC
GCAAGTTATGCAGATGAGATTTACCTCAATCCCCGTGGTGAAGTCTTTATCGACGGTTACGCCGCATATAACCTCTATTT
CAAGTCGGCTCTGGAGAAACTTAAGGTTAACACCCACGTCTTCCGCGTAGGTACCTATAAGTCAGCGGTTGAACCTTATA
TCCGCGACGATATGTCACCGGCGGCAAAAGAAGCCACCCAACTCTTGATTGACGATCTCTGGGGTAGCTATGCCGACACG
GTTGCGAAAAACCGCGGCATCGAAACCGGTGAACTGGTATTGGATGCGGACACTTATCTTGCTCGCTTGGACGCCAACGA
CGGTGACAGCGCTGCGCTTGCTGTTAAGCAGGGTTGGGTAGACGCCCTGATGAGTGACGAGGCGTTTCGCTCCGCCATGG
TTGAACTCGTTGGCGAAGACAAAGAAGAGCACAGCTTCCGTCAGGTGGGTTTCCACGAGTACCATAAACTGGTGAAACCT
CAGCCACAGTTTATGCCGGTAGACAGCGTGGGCGTTATCGTTGCCAAAGGCACCATACTCAACGGTTTCCAGGCGCCTGG
CGACATCGGTGGCAAGAGTACATCCCAGTTAATTCGTGATGCCCGTTTTGATGATTCCATCAAGGCCTTGGTGTTACGTG
TAGACAGCCCCGGCGGAAGTGCCTTTGCCTCAGAAGAAATACGCCAGGAGCTTCTGGCACTCAAGGCAGCGGGTAAACCT
GTAGTGGTGAGCATGGGCAGTATGGCGGCCTCCGGGGGTTACTGGATTTCAGCCAGCGCCGATTACATTTATGCCACGCC
CACGACGCTTACAGGCTCCATTGGTATCTTTGGCTTGATGACCACTTTTGAAGACTCATTGGCAGCCATTGGTGTGCATG
CCGACGGGGTCGCCACCAGTGAATGGGCCGCACACTCACCCTTCCGTCCGTTGTCACCCAAGCTTGAATCGGCCATTCAG
CGCAATATCGAGCGCGGTTACAAGGAATTTATCACTCTGGTCGCCAACGAACGCGACATGACGCTGGAACAGGTCGATGC
CATCGCTCAGGGTCGCGTGTGGAGCGGTAAAAAGGCGCTGGAACTTGGCCTGGTAGACGAGATTGGTGATATGCCTCAGG
CACTGGCCAAAGCAGCCGAACTTGCTGGCATGGCGACCTTTGACATTCAGTTGATTGAACAGCAGATGTCGCCAGAAGAG
CTGTTTATTCAGGAAATGTTTGCCTCTGTGTCGGCCTGGCTGCCACAGCAGACACAACAGAGTGACGTGGTGCAATCCTT
GCTGCGTGTTTGGACCAGGACGTTGCAGGAAATCGCCCGCTTTGACGACCCCAAGGGGGTATACCTCTACTGCGAATATT
GCCAGCTGCAATAG

Upstream 100 bases:

>100_bases
CCGTCCAGCCCACTGAATGTTAAAAACCTTTACAATTGCATTTCGCCGCAATGAGTTATTTGCGGTAGCATATGCCCAGC
GTCAACTATAGGAGTGCTGA

Downstream 100 bases:

>100_bases
CTGCAATAAGTCAGCGCTGAACAGTAAAAGCCCGGCTCATGCCGGGTTTTTCTTTGGCTGTCTTACTTTATCAGTGAATT
TTTAACTGAATTAGCCCCTG

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MAKKPLTTKNTLLLIWNTINFVRKLIINIVFFPILILVLIVGIVALSTEDELRIESGSALVLDLSGNLVDQARRVDPLTQ
LMRQGNQNSEDTEVLLSDVLYVIENATHDERIGAIVLDLGNMRAGISKLTAVGEALNGFRETGKKVVAIGDWYSRSQYFL
ASYADEIYLNPRGEVFIDGYAAYNLYFKSALEKLKVNTHVFRVGTYKSAVEPYIRDDMSPAAKEATQLLIDDLWGSYADT
VAKNRGIETGELVLDADTYLARLDANDGDSAALAVKQGWVDALMSDEAFRSAMVELVGEDKEEHSFRQVGFHEYHKLVKP
QPQFMPVDSVGVIVAKGTILNGFQAPGDIGGKSTSQLIRDARFDDSIKALVLRVDSPGGSAFASEEIRQELLALKAAGKP
VVVSMGSMAASGGYWISASADYIYATPTTLTGSIGIFGLMTTFEDSLAAIGVHADGVATSEWAAHSPFRPLSPKLESAIQ
RNIERGYKEFITLVANERDMTLEQVDAIAQGRVWSGKKALELGLVDEIGDMPQALAKAAELAGMATFDIQLIEQQMSPEE
LFIQEMFASVSAWLPQQTQQSDVVQSLLRVWTRTLQEIARFDDPKGVYLYCEYCQLQ

Sequences:

>Translated_617_residues
MAKKPLTTKNTLLLIWNTINFVRKLIINIVFFPILILVLIVGIVALSTEDELRIESGSALVLDLSGNLVDQARRVDPLTQ
LMRQGNQNSEDTEVLLSDVLYVIENATHDERIGAIVLDLGNMRAGISKLTAVGEALNGFRETGKKVVAIGDWYSRSQYFL
ASYADEIYLNPRGEVFIDGYAAYNLYFKSALEKLKVNTHVFRVGTYKSAVEPYIRDDMSPAAKEATQLLIDDLWGSYADT
VAKNRGIETGELVLDADTYLARLDANDGDSAALAVKQGWVDALMSDEAFRSAMVELVGEDKEEHSFRQVGFHEYHKLVKP
QPQFMPVDSVGVIVAKGTILNGFQAPGDIGGKSTSQLIRDARFDDSIKALVLRVDSPGGSAFASEEIRQELLALKAAGKP
VVVSMGSMAASGGYWISASADYIYATPTTLTGSIGIFGLMTTFEDSLAAIGVHADGVATSEWAAHSPFRPLSPKLESAIQ
RNIERGYKEFITLVANERDMTLEQVDAIAQGRVWSGKKALELGLVDEIGDMPQALAKAAELAGMATFDIQLIEQQMSPEE
LFIQEMFASVSAWLPQQTQQSDVVQSLLRVWTRTLQEIARFDDPKGVYLYCEYCQLQ
>Mature_616_residues
AKKPLTTKNTLLLIWNTINFVRKLIINIVFFPILILVLIVGIVALSTEDELRIESGSALVLDLSGNLVDQARRVDPLTQL
MRQGNQNSEDTEVLLSDVLYVIENATHDERIGAIVLDLGNMRAGISKLTAVGEALNGFRETGKKVVAIGDWYSRSQYFLA
SYADEIYLNPRGEVFIDGYAAYNLYFKSALEKLKVNTHVFRVGTYKSAVEPYIRDDMSPAAKEATQLLIDDLWGSYADTV
AKNRGIETGELVLDADTYLARLDANDGDSAALAVKQGWVDALMSDEAFRSAMVELVGEDKEEHSFRQVGFHEYHKLVKPQ
PQFMPVDSVGVIVAKGTILNGFQAPGDIGGKSTSQLIRDARFDDSIKALVLRVDSPGGSAFASEEIRQELLALKAAGKPV
VVSMGSMAASGGYWISASADYIYATPTTLTGSIGIFGLMTTFEDSLAAIGVHADGVATSEWAAHSPFRPLSPKLESAIQR
NIERGYKEFITLVANERDMTLEQVDAIAQGRVWSGKKALELGLVDEIGDMPQALAKAAELAGMATFDIQLIEQQMSPEEL
FIQEMFASVSAWLPQQTQQSDVVQSLLRVWTRTLQEIARFDDPKGVYLYCEYCQLQ

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=604, Percent_Identity=45.364238410596, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1787527, Length=172, Percent_Identity=29.0697674418605, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 67825; Mature: 67694

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPLTTKNTLLLIWNTINFVRKLIINIVFFPILILVLIVGIVALSTEDELRIESGSAL
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEE
VLDLSGNLVDQARRVDPLTQLMRQGNQNSEDTEVLLSDVLYVIENATHDERIGAIVLDLG
EEEECCCHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCEEEEECC
NMRAGISKLTAVGEALNGFRETGKKVVAIGDWYSRSQYFLASYADEIYLNPRGEVFIDGY
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEECCCCCEEECCH
AAYNLYFKSALEKLKVNTHVFRVGTYKSAVEPYIRDDMSPAAKEATQLLIDDLWGSYADT
HHHHHHHHHHHHHHHCCCEEEEECCHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VAKNRGIETGELVLDADTYLARLDANDGDSAALAVKQGWVDALMSDEAFRSAMVELVGED
HHHHCCCCCCCEEEECCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
KEEHSFRQVGFHEYHKLVKPQPQFMPVDSVGVIVAKGTILNGFQAPGDIGGKSTSQLIRD
CHHHHHHHCCHHHHHHHCCCCCCEECCCCCCEEEECCEEECCCCCCCCCCCCHHHHHHHH
ARFDDSIKALVLRVDSPGGSAFASEEIRQELLALKAAGKPVVVSMGSMAASGGYWISASA
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEECC
DYIYATPTTLTGSIGIFGLMTTFEDSLAAIGVHADGVATSEWAAHSPFRPLSPKLESAIQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
RNIERGYKEFITLVANERDMTLEQVDAIAQGRVWSGKKALELGLVDEIGDMPQALAKAAE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHEECCHHHHCCHHHHHHHHHH
LAGMATFDIQLIEQQMSPEELFIQEMFASVSAWLPQQTQQSDVVQSLLRVWTRTLQEIAR
HCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
FDDPKGVYLYCEYCQLQ
CCCCCCEEEEEEEEECC
>Mature Secondary Structure 
AKKPLTTKNTLLLIWNTINFVRKLIINIVFFPILILVLIVGIVALSTEDELRIESGSAL
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEE
VLDLSGNLVDQARRVDPLTQLMRQGNQNSEDTEVLLSDVLYVIENATHDERIGAIVLDLG
EEEECCCHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCEEEEECC
NMRAGISKLTAVGEALNGFRETGKKVVAIGDWYSRSQYFLASYADEIYLNPRGEVFIDGY
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCEEECCCCCEEECCH
AAYNLYFKSALEKLKVNTHVFRVGTYKSAVEPYIRDDMSPAAKEATQLLIDDLWGSYADT
HHHHHHHHHHHHHHHCCCEEEEECCHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VAKNRGIETGELVLDADTYLARLDANDGDSAALAVKQGWVDALMSDEAFRSAMVELVGED
HHHHCCCCCCCEEEECCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
KEEHSFRQVGFHEYHKLVKPQPQFMPVDSVGVIVAKGTILNGFQAPGDIGGKSTSQLIRD
CHHHHHHHCCHHHHHHHCCCCCCEECCCCCCEEEECCEEECCCCCCCCCCCCHHHHHHHH
ARFDDSIKALVLRVDSPGGSAFASEEIRQELLALKAAGKPVVVSMGSMAASGGYWISASA
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCEEEEECC
DYIYATPTTLTGSIGIFGLMTTFEDSLAAIGVHADGVATSEWAAHSPFRPLSPKLESAIQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
RNIERGYKEFITLVANERDMTLEQVDAIAQGRVWSGKKALELGLVDEIGDMPQALAKAAE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHEECCHHHHCCHHHHHHHHHH
LAGMATFDIQLIEQQMSPEELFIQEMFASVSAWLPQQTQQSDVVQSLLRVWTRTLQEIAR
HCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
FDDPKGVYLYCEYCQLQ
CCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]