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The map label for this gene is adhE [H]

Identifier: 119774829

GI number: 119774829

Start: 2070919

End: 2073522

Strand: Reverse

Name: adhE [H]

Synonym: Sama_1693

Alternate gene names: 119774829

Gene position: 2073522-2070919 (Counterclockwise)

Preceding gene: 119774831

Following gene: 119774823

Centisome position: 48.15

GC content: 55.68

Gene sequence:

>2604_bases
ATGACAGTCACCAACACTCAGGAACTCAACGAACTGGTCGCGCGCGTCGCCAAGGCACAGGCCCAATTTGCTTCTTACAG
CCAAGAGCAAGTGGACAGGATTTTCCGCGCCGCCGCCCTCGCCGCTGCTGATGCACGCATTCGTCTGGCCAAAATGGCCG
CTGAAGAAACCCGCATGGGGGTTGTTGAAGATAAGGTCATTAAAAACCATTTCGCCTCCGAGTACATTTACAACAAATAC
AAAGACGAAAAGACCTGCGGCATTCTGGCCGAAGACGCCACCTTCGGCACTATCACCATCGCCGAGCCGGTTGGGATCAT
CTGCGGCATTGTGCCCACCACCAACCCGACCTCCACCGCTATCTTTAAAGCGCTGATAAGCTTAAAGACCCGCAACGGCA
TTATCTTCTCGCCTCACCCAAGGGCCAAGGTTTCAACTACCACTGCCGCCCGCATCGTACTGGATGCAGCCATTGCAGCA
GGTGCCCCGAAAGACATTATTGGCTGGATTGATGAGCCTTCGGTGGCGCTGTCCAACCAGTTGATGACCCACGAGAAGAT
TAACCTTATTCTCGCCACCGGCGGCCCCGGCATGGTGAAAGCGGCCTACTCCTCCGGCAAACCCGCGATTGGCGTTGGCG
CCGGCAACACCCCCATCGTGATTGATGAGACCGCTGACATCAAACGTGCTGTAAGCTCAATTTTGATGTCCAAGACCTTC
GATAACGGCGTGGTGTGTGCCTCCGAGCAAGCGGTTGTGGTGGTGGATGCGGTTTACGATGCGGTAAAAGAGCGCTTCTC
GAGCCACGGCGGCTATCTGCTCAGCAAGAAAGAAAACGCCGCGCTGCAAAAAGTCATCCTCAAAGACGGTGGCCTCAATG
CCGATATCGTGGGTCAAAGCGCCGCCACCATTGCCGCCATGGCCAATATCAAGGTGCCTGCCCACACCAAGGTGCTGATT
GGTGAAGTCACCGACATCGATGAGAAAGAGGCCTTCGCCCACGAGAAACTCTCACCGCTGCTTGGCATGTACCGCGCCGC
CAACTTTGAAGAGGCGCTGGACAAGGCCGAAGCCCTGGTTGCTCTTGGCGGTATTGGTCACACCTCAGGCCTGTATACCG
ATCAGGATACCCAGGACGAGCGGGTCAAATCCTTCGGCTACCGGATGAAGACCGCCCGTATTCTGATTAACACCCCGGCC
TCTCAGGGCGGCATAGGCGATTTATACAACTTCAAGCTGGCGCCTTCATTGACTCTGGGCTGCGGCTCCTGGGGCGGTAA
CTCGATTTCTGAAAACGTAGGTCCAAGCCATCTTATCAACAAGAAAACCGTCGCCAAGAGGGCCGAAAATATGCTGTGGC
ACAAGCTTCCTTCGTCCATCTACTTCCGCCGTGGCAGCCTGCCGATTGCCCTCGAAGAGCTGAGTGGCAAGAAGCGTGCG
CTTATCGTGACCGACAAGTTTCTGTTCAACAACGGCTATTGCGATGAAACCATCCGAATTCTGAAGTCACAGGGCCTGGA
AACCGAGGTCTTTTACGAAGTGGAAGCCGACCCAACCTTAGCGGTGGTTCGCGCCGGAGCCAAAGTGGCAACCAGCTTCC
AACCTGATGTGATAGTGGCTCTGGGTGGTGGTTCGCCCATGGATGCGGCCAAGATCATTTGGGTAATGTACGAGCATCCC
GATGTGGACTTTGCCGACCTGGCGCTGCGCTTTATGGATATCCGTAAGCGTATCTACAAGTTCCCCAAACTTGGTGCCAA
GGCAATGATGGTGGCCATTCCCACCACCTCAGGTACTGGCTCTGAAGTCACGCCTTTTGCGGTGGTGACCGACGAGCAAA
CCGGTGCCAAATACCCCATTGCCGACTATGAACTGACCCCCAACATGGCCATTGTCGACCCCAATCTGGTAATGGATATG
CCCAAGTCGCTCACCGCTTTCGGCGGTATCGATGCCATTACCCACGCACTGGAAGCCTATGTCAGCGTGATGGCCAACGA
ATACAGCGACGGCCAGGCACTGCAGGCACTGGATTTACTGTTTAAGTATCTGCCAGACAGCTACGCCAGGGGCGCGCAGG
CGCCGCTGGCACGGGAAAAAGTGCACAATGGCGCCACCATTGCCGGTATCGCTTTTGCCAACGCCTTCTTAGGCATTTGC
CACTCAATGGCTCACAAATTGGGCGCTGAGTTTCACCTGCCCCATGGCCTTGCCAATGCGCTCCTTATCAGCAACGTCAT
TCGCTTCAATGCCACTGACCTGCCCACCAAACAGGCGGCGTTCAGCCAGTACGACAGACCCAAGGCCCTGTGCCGTTATG
CCGCCATTGCCAGCCACTTAGGCCTTGCCGGCAACAATGATGAAGCCAAGGTAGAGGCGCTTATCGCCAAGATTGAGGAG
CTTAAAGCCGCCATCGGTATTCCGGTGTCTATCAAGGACGCAGGGGTAAATGAAGCCGACTTTATGGCCAAGCTTGATGA
GTTGGCCGAAGATGCCTTTGACGATCAGTGCACCGGCGCCAACCCGCGCTACCCACTGATTAGCGAGCTGAAACAGCTGC
TTATCGATAGCTTCCATGGACGTGCCTATCAGGACAGTCTCTGA

Upstream 100 bases:

>100_bases
CACGGTCGCCATACCTATATAGGTGTAATTTTCAGTCACAGGCAAGAATCTTTAGCTAAATTCAGTTTTTGCTAAACATT
CTTTTTACGGGAACTTCATT

Downstream 100 bases:

>100_bases
TGCATAAAGGGTGACACCGCCCCTTAACACAAAAAAGCCCGGCAACCGCCGGGCTTTTTCATTCGACCATTTCTGGATCA
GCAGATAGTCACGAGATCGT

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 867; Mature: 866

Protein sequence:

>867_residues
MTVTNTQELNELVARVAKAQAQFASYSQEQVDRIFRAAALAAADARIRLAKMAAEETRMGVVEDKVIKNHFASEYIYNKY
KDEKTCGILAEDATFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKVSTTTAARIVLDAAIAA
GAPKDIIGWIDEPSVALSNQLMTHEKINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
DNGVVCASEQAVVVVDAVYDAVKERFSSHGGYLLSKKENAALQKVILKDGGLNADIVGQSAATIAAMANIKVPAHTKVLI
GEVTDIDEKEAFAHEKLSPLLGMYRAANFEEALDKAEALVALGGIGHTSGLYTDQDTQDERVKSFGYRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSGKKRA
LIVTDKFLFNNGYCDETIRILKSQGLETEVFYEVEADPTLAVVRAGAKVATSFQPDVIVALGGGSPMDAAKIIWVMYEHP
DVDFADLALRFMDIRKRIYKFPKLGAKAMMVAIPTTSGTGSEVTPFAVVTDEQTGAKYPIADYELTPNMAIVDPNLVMDM
PKSLTAFGGIDAITHALEAYVSVMANEYSDGQALQALDLLFKYLPDSYARGAQAPLAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLPHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIASHLGLAGNNDEAKVEALIAKIEE
LKAAIGIPVSIKDAGVNEADFMAKLDELAEDAFDDQCTGANPRYPLISELKQLLIDSFHGRAYQDSL

Sequences:

>Translated_867_residues
MTVTNTQELNELVARVAKAQAQFASYSQEQVDRIFRAAALAAADARIRLAKMAAEETRMGVVEDKVIKNHFASEYIYNKY
KDEKTCGILAEDATFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKVSTTTAARIVLDAAIAA
GAPKDIIGWIDEPSVALSNQLMTHEKINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
DNGVVCASEQAVVVVDAVYDAVKERFSSHGGYLLSKKENAALQKVILKDGGLNADIVGQSAATIAAMANIKVPAHTKVLI
GEVTDIDEKEAFAHEKLSPLLGMYRAANFEEALDKAEALVALGGIGHTSGLYTDQDTQDERVKSFGYRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSGKKRA
LIVTDKFLFNNGYCDETIRILKSQGLETEVFYEVEADPTLAVVRAGAKVATSFQPDVIVALGGGSPMDAAKIIWVMYEHP
DVDFADLALRFMDIRKRIYKFPKLGAKAMMVAIPTTSGTGSEVTPFAVVTDEQTGAKYPIADYELTPNMAIVDPNLVMDM
PKSLTAFGGIDAITHALEAYVSVMANEYSDGQALQALDLLFKYLPDSYARGAQAPLAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLPHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIASHLGLAGNNDEAKVEALIAKIEE
LKAAIGIPVSIKDAGVNEADFMAKLDELAEDAFDDQCTGANPRYPLISELKQLLIDSFHGRAYQDSL
>Mature_866_residues
TVTNTQELNELVARVAKAQAQFASYSQEQVDRIFRAAALAAADARIRLAKMAAEETRMGVVEDKVIKNHFASEYIYNKYK
DEKTCGILAEDATFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNGIIFSPHPRAKVSTTTAARIVLDAAIAAG
APKDIIGWIDEPSVALSNQLMTHEKINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTFD
NGVVCASEQAVVVVDAVYDAVKERFSSHGGYLLSKKENAALQKVILKDGGLNADIVGQSAATIAAMANIKVPAHTKVLIG
EVTDIDEKEAFAHEKLSPLLGMYRAANFEEALDKAEALVALGGIGHTSGLYTDQDTQDERVKSFGYRMKTARILINTPAS
QGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSGKKRAL
IVTDKFLFNNGYCDETIRILKSQGLETEVFYEVEADPTLAVVRAGAKVATSFQPDVIVALGGGSPMDAAKIIWVMYEHPD
VDFADLALRFMDIRKRIYKFPKLGAKAMMVAIPTTSGTGSEVTPFAVVTDEQTGAKYPIADYELTPNMAIVDPNLVMDMP
KSLTAFGGIDAITHALEAYVSVMANEYSDGQALQALDLLFKYLPDSYARGAQAPLAREKVHNGATIAGIAFANAFLGICH
SMAHKLGAEFHLPHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIASHLGLAGNNDEAKVEALIAKIEEL
KAAIGIPVSIKDAGVNEADFMAKLDELAEDAFDDQCTGANPRYPLISELKQLLIDSFHGRAYQDSL

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=315, Percent_Identity=27.9365079365079, Blast_Score=87, Evalue=8e-17,
Organism=Escherichia coli, GI1787493, Length=867, Percent_Identity=77.1626297577855, Blast_Score=1360, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=391, Percent_Identity=36.8286445012788, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1789163, Length=402, Percent_Identity=32.8358208955224, Blast_Score=197, Evalue=3e-51,
Organism=Escherichia coli, GI87082107, Length=380, Percent_Identity=31.8421052631579, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI1788797, Length=367, Percent_Identity=32.9700272479564, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789386, Length=388, Percent_Identity=25.2577319587629, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=306, Percent_Identity=28.1045751633987, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=391, Percent_Identity=33.7595907928389, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24657991, Length=327, Percent_Identity=26.605504587156, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 93035; Mature: 92903

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTNTQELNELVARVAKAQAQFASYSQEQVDRIFRAAALAAADARIRLAKMAAEETRMG
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VVEDKVIKNHFASEYIYNKYKDEKTCGILAEDATFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNGIIFSPHPRAKVSTTTAARIVLDAAIAAGAPKDIIGWIDEPSVALSNQ
HHHHHHHHHHCCCEEECCCCCCEECHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMTHEKINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
HHCEEEEEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVVCASEQAVVVVDAVYDAVKERFSSHGGYLLSKKENAALQKVILKDGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCEEECCC
AATIAAMANIKVPAHTKVLIGEVTDIDEKEAFAHEKLSPLLGMYRAANFEEALDKAEALV
HHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALGGIGHTSGLYTDQDTQDERVKSFGYRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
HCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSGKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCEE
LIVTDKFLFNNGYCDETIRILKSQGLETEVFYEVEADPTLAVVRAGAKVATSFQPDVIVA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHCCCCCCCEEEE
LGGGSPMDAAKIIWVMYEHPDVDFADLALRFMDIRKRIYKFPKLGAKAMMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGAKYPIADYELTPNMAIVDPNLVMDMPKSLTAFGGIDAITHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEECCHHHHHCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLFKYLPDSYARGAQAPLAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLPHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIASHL
HHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHC
GLAGNNDEAKVEALIAKIEELKAAIGIPVSIKDAGVNEADFMAKLDELAEDAFDDQCTGA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
NPRYPLISELKQLLIDSFHGRAYQDSL
CCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVTNTQELNELVARVAKAQAQFASYSQEQVDRIFRAAALAAADARIRLAKMAAEETRMG
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VVEDKVIKNHFASEYIYNKYKDEKTCGILAEDATFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNGIIFSPHPRAKVSTTTAARIVLDAAIAAGAPKDIIGWIDEPSVALSNQ
HHHHHHHHHHCCCEEECCCCCCEECHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMTHEKINLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
HHCEEEEEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVVCASEQAVVVVDAVYDAVKERFSSHGGYLLSKKENAALQKVILKDGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCEEECCC
AATIAAMANIKVPAHTKVLIGEVTDIDEKEAFAHEKLSPLLGMYRAANFEEALDKAEALV
HHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALGGIGHTSGLYTDQDTQDERVKSFGYRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
HCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSGKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCEE
LIVTDKFLFNNGYCDETIRILKSQGLETEVFYEVEADPTLAVVRAGAKVATSFQPDVIVA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCHHCCCCCCCEEEE
LGGGSPMDAAKIIWVMYEHPDVDFADLALRFMDIRKRIYKFPKLGAKAMMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGAKYPIADYELTPNMAIVDPNLVMDMPKSLTAFGGIDAITHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEECCHHHHHCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLFKYLPDSYARGAQAPLAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLPHGLANALLISNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIASHL
HHHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHC
GLAGNNDEAKVEALIAKIEELKAAIGIPVSIKDAGVNEADFMAKLDELAEDAFDDQCTGA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
NPRYPLISELKQLLIDSFHGRAYQDSL
CCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]