Click here to switch to the map view.

The map label for this gene is hydC [H]

Identifier: 119774813

GI number: 119774813

Start: 2051003

End: 2051719

Strand: Reverse

Name: hydC [H]

Synonym: Sama_1677

Alternate gene names: 119774813

Gene position: 2051719-2051003 (Counterclockwise)

Preceding gene: 119774814

Following gene: 119774812

Centisome position: 47.65

GC content: 53.56

Gene sequence:

>717_bases
ATGAACACCCAAGTTAATACGCCGCCTGCGGGCGGCAAGCATGACACGAAGCACGAGGTGCATACCGGAGGGTACCAGCG
GGAGTTTATCTTCACTGCCGCTATCCGACTCTTCCACTGGCTAAGGGCGCTGGCGATTTTGATCCTGGTTATCACAGGTT
TCTACCTTGCCTGGCCATTTTTGGTGGCACCTGACTCCAGCGATGTACTGGTTCAGGGCTGGGTACGGTTTGGTCACCTG
GTGTTCGGCTTTGTGCTGTGCGCCGTGACCCTGGCCCGCGCTTATCTGTTCTTTTTCAGTAAGCACGATATTGAGCGGCG
ATCCATCCGCGACGTGCTCAGCATCAACAGCTGGATTACCCAACTTAAGTCCTACCTCTGGATGGGTAAGCTGAACAAGG
CGGGGGTGTATGGACCACTGCAATTTGTGACCTACTTTGCGATTTCGGTGATTGCCGGCGCAATCTGTGTCACAGGGCTG
ATACTGTATGCCAATGTTTATCATGAAGGCATGGGTGGCATGCTCTGGGGCATGGCGAGCACCCTGACCGCCTGGATGGG
TGGCCTTGCCGAAGTGCGGCTGTGGCATCATTACTTTACCTGGGTATTTATTGTATTCGTGGTCATCCACGTTTACATGG
CTGTGTGGACTGGCATTCGCTTTAAGCACAACTCGGTAGACTCCATCGTCTCCGGATATGACTACCACAAGCATTAA

Upstream 100 bases:

>100_bases
CTTCGACCCCTGCATGGCTTGCTCGGTACACGTAATGGACTTCAAGGGTGCAAGCCTGTCAGAGTTCAAGGTGTCACCAA
ACGGGCTCTAAGGAGGCAGT

Downstream 100 bases:

>100_bases
TCCGCGTAATGATGACTTTGCGCCATACCACAAGGGAAACCGGCGGTATGGCGCACCTTACCCCCGCAGGCATTGCTATC
CCGACTATTACCCGGCGCCT

Product: quinone-reactive Ni/Fe hydrogenase, cytochrome subunit B

Products: oxidized ferredoxin; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNTQVNTPPAGGKHDTKHEVHTGGYQREFIFTAAIRLFHWLRALAILILVITGFYLAWPFLVAPDSSDVLVQGWVRFGHL
VFGFVLCAVTLARAYLFFFSKHDIERRSIRDVLSINSWITQLKSYLWMGKLNKAGVYGPLQFVTYFAISVIAGAICVTGL
ILYANVYHEGMGGMLWGMASTLTAWMGGLAEVRLWHHYFTWVFIVFVVIHVYMAVWTGIRFKHNSVDSIVSGYDYHKH

Sequences:

>Translated_238_residues
MNTQVNTPPAGGKHDTKHEVHTGGYQREFIFTAAIRLFHWLRALAILILVITGFYLAWPFLVAPDSSDVLVQGWVRFGHL
VFGFVLCAVTLARAYLFFFSKHDIERRSIRDVLSINSWITQLKSYLWMGKLNKAGVYGPLQFVTYFAISVIAGAICVTGL
ILYANVYHEGMGGMLWGMASTLTAWMGGLAEVRLWHHYFTWVFIVFVVIHVYMAVWTGIRFKHNSVDSIVSGYDYHKH
>Mature_238_residues
MNTQVNTPPAGGKHDTKHEVHTGGYQREFIFTAAIRLFHWLRALAILILVITGFYLAWPFLVAPDSSDVLVQGWVRFGHL
VFGFVLCAVTLARAYLFFFSKHDIERRSIRDVLSINSWITQLKSYLWMGKLNKAGVYGPLQFVTYFAISVIAGAICVTGL
ILYANVYHEGMGGMLWGMASTLTAWMGGLAEVRLWHHYFTWVFIVFVVIHVYMAVWTGIRFKHNSVDSIVSGYDYHKH

Specific function: B-type cytochrome that interacts with quinones and anchor the hydrogenase to the membrane [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=220, Percent_Identity=24.5454545454545, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: NA

Molecular weight: Translated: 27111; Mature: 27111

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00882 NI_HGENASE_CYTB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQVNTPPAGGKHDTKHEVHTGGYQREFIFTAAIRLFHWLRALAILILVITGFYLAWPF
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAPDSSDVLVQGWVRFGHLVFGFVLCAVTLARAYLFFFSKHDIERRSIRDVLSINSWIT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QLKSYLWMGKLNKAGVYGPLQFVTYFAISVIAGAICVTGLILYANVYHEGMGGMLWGMAS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLTAWMGGLAEVRLWHHYFTWVFIVFVVIHVYMAVWTGIRFKHNSVDSIVSGYDYHKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MNTQVNTPPAGGKHDTKHEVHTGGYQREFIFTAAIRLFHWLRALAILILVITGFYLAWPF
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAPDSSDVLVQGWVRFGHLVFGFVLCAVTLARAYLFFFSKHDIERRSIRDVLSINSWIT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QLKSYLWMGKLNKAGVYGPLQFVTYFAISVIAGAICVTGLILYANVYHEGMGGMLWGMAS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLTAWMGGLAEVRLWHHYFTWVFIVFVVIHVYMAVWTGIRFKHNSVDSIVSGYDYHKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; 2 H+ [C]

Specific reaction: Reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]