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The map label for this gene is hydD [H]

Identifier: 119774812

GI number: 119774812

Start: 2050294

End: 2050851

Strand: Reverse

Name: hydD [H]

Synonym: Sama_1676

Alternate gene names: 119774812

Gene position: 2050851-2050294 (Counterclockwise)

Preceding gene: 119774813

Following gene: 119774811

Centisome position: 47.63

GC content: 50.9

Gene sequence:

>558_bases
ATGAAAGTCCTGCTACTCGGCATTGGCAATATACTCTTCGCCGACGAAGGCGTAGGCGTACACTTTGTCAATTATATATC
TGAAAATTATCGCTTTACCCACCCGCTCCACTCCCTTGATATTCTCGATGGCGGTACTCTGGCCCAGGGCCTGACTCCCA
TTATTGCCAAATACGACCATTTGATTGTGTGTGATACGGTCAACGCTCCCGGGGTTTCACCCGGCGAAGTCTATTTTTTT
GATTTTGATGCAGCGCCCGCTGAAATTGACTGGCAAGGCAGCGCCCACGAAGTCGAAATGCTGCAAACACTCACCATGAT
GGAGATGCTGGGCGACCGGCCGAAAACCTTTGTACTGGGTGTCACCCCTACCGTGCTGGAACCCATGCATCTGGGACTTA
CCGAGGCACTTGGCAGCGCTATTCCGGTAATGGAACAGGCGCTGATTAACCACCTGAAGCAATTGGGTTTCCAGTCTGAA
CGCATCGCCAATAAACCCATTACCAGTTTTTACCAAGACGGGATCACCCGCGAGGTATCAGTGGATGAAAACCATTAG

Upstream 100 bases:

>100_bases
CGCACCTTACCCCCGCAGGCATTGCTATCCCGACTATTACCCGGCGCCTTTGTAACAATTTCGGTGCAGCCCTCGTTGCG
AGGCAATTGGAGGACAAAGG

Downstream 100 bases:

>100_bases
TTTTGAATTTGTCACATCCCGGGCCGTGCCCTTTTATCATCAAACTCTGGCTGCACTGGTAAACCAAGGCGCGCCCAATG
TTGAGCTTAGCGCCCGGATC

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKVLLLGIGNILFADEGVGVHFVNYISENYRFTHPLHSLDILDGGTLAQGLTPIIAKYDHLIVCDTVNAPGVSPGEVYFF
DFDAAPAEIDWQGSAHEVEMLQTLTMMEMLGDRPKTFVLGVTPTVLEPMHLGLTEALGSAIPVMEQALINHLKQLGFQSE
RIANKPITSFYQDGITREVSVDENH

Sequences:

>Translated_185_residues
MKVLLLGIGNILFADEGVGVHFVNYISENYRFTHPLHSLDILDGGTLAQGLTPIIAKYDHLIVCDTVNAPGVSPGEVYFF
DFDAAPAEIDWQGSAHEVEMLQTLTMMEMLGDRPKTFVLGVTPTVLEPMHLGLTEALGSAIPVMEQALINHLKQLGFQSE
RIANKPITSFYQDGITREVSVDENH
>Mature_185_residues
MKVLLLGIGNILFADEGVGVHFVNYISENYRFTHPLHSLDILDGGTLAQGLTPIIAKYDHLIVCDTVNAPGVSPGEVYFF
DFDAAPAEIDWQGSAHEVEMLQTLTMMEMLGDRPKTFVLGVTPTVLEPMHLGLTEALGSAIPVMEQALINHLKQLGFQSE
RIANKPITSFYQDGITREVSVDENH

Specific function: Protease Involved In The C-Terminal Processing Of Hybc, The Large Subunit Of Hydrogenase 2. Specifically Cleaves Off A 15 Amino Acid Peptide From The C Terminus Of The Precursor Of Hybc. [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=161, Percent_Identity=34.1614906832298, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 20351; Mature: 20351

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLLGIGNILFADEGVGVHFVNYISENYRFTHPLHSLDILDGGTLAQGLTPIIAKYDH
CEEEEEECCCEEEECCCCCCHHHHHHHCCCEEECCCCEEEECCCCCHHHHHHHHHHCCCC
LIVCDTVNAPGVSPGEVYFFDFDAAPAEIDWQGSAHEVEMLQTLTMMEMLGDRPKTFVLG
EEEEECCCCCCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
VTPTVLEPMHLGLTEALGSAIPVMEQALINHLKQLGFQSERIANKPITSFYQDGITREVS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHCCCCEEEE
VDENH
CCCCC
>Mature Secondary Structure
MKVLLLGIGNILFADEGVGVHFVNYISENYRFTHPLHSLDILDGGTLAQGLTPIIAKYDH
CEEEEEECCCEEEECCCCCCHHHHHHHCCCEEECCCCEEEECCCCCHHHHHHHHHHCCCC
LIVCDTVNAPGVSPGEVYFFDFDAAPAEIDWQGSAHEVEMLQTLTMMEMLGDRPKTFVLG
EEEEECCCCCCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
VTPTVLEPMHLGLTEALGSAIPVMEQALINHLKQLGFQSERIANKPITSFYQDGITREVS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHCCCCEEEE
VDENH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1587288; 9639603; 14500908 [H]