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The map label for this gene is zraR [H]

Identifier: 119774555

GI number: 119774555

Start: 1722268

End: 1723611

Strand: Reverse

Name: zraR [H]

Synonym: Sama_1418

Alternate gene names: 119774555

Gene position: 1723611-1722268 (Counterclockwise)

Preceding gene: 119774556

Following gene: 119774554

Centisome position: 40.03

GC content: 52.38

Gene sequence:

>1344_bases
ATGAACAAAGTGGTGCAAAAGGAAACCCGCGCTGCTGGCACAATGCCCCGCCATCTCTTGCTCCTGTCAAAATCCCAGGA
AGTAACAGATGTAAGTCGTCGCCTCGCGGCATACGGCTGGCATTGTCTGACTGCAGCCAGCGGTCATGAAGCAATTTTAA
AACTGAGCGGACAGCAGTTGGGAGTCGCCGTCGCCATGCTGTGTCCCCGTGCCGATACCCTGTCTTCAGACATCGCCCTC
ATTCAAAGCCACTTTCCGCACTTACTCTGGATAGCCCTCACCGACGGTAAACTCGATAAGGCATGCCAGTGGTTGCTCAG
CGCCCGCTTTATCGACTATCACCATTCTCCCCTTGACTGGGACAGCGTCAACAAAGCCCTGGGCCACGCCGACGGCATGG
CAAAATTACTGCCGGTAAAAACCGCAGGGGTTAAAATTACCGATATTTTGGGCACACACCCCTGTATCATCCGTCTGAAA
CAAGACATCCAAAAACTCGCCAGTACAGACGAAACAGTACTGCTCAGCGGTGAAACCGGCACAGGCAAAGGTCTGTGTGC
TAAATGGCTGCACCGAATGTCGGCAAGAGCCGATGGACCTTTTATTGCCCTTAATTGCGGCGCCTTACCCAACGGCCTTA
TCCAATCCACCCTGTTTGGCCACGAAAAGGGGGCCTTTACCGGCGCAGACAGACAGTATCAGGGTCACATCGAACAAGCC
AAAGACGGGGTATTGTTCCTGGATGAAATCGCCGATCTGCCACTGGAGCTGCAAGTTAACCTGCTCCATTTTCTTGATGA
CAGACAAATCACCCGTATCGGTGGCAAGAGCCCACTGAAGATAAACTGCCGCATTATCTTTGCGACGCATCAAAACCTTG
AAAAAGCGGTCGATGACGGTACGTTCCGGGAAGACCTCTACCACAGAATTAATGTGCTCAGGCTGCATGTACCCAGCCTG
CGAGAGTACTGCACAGAAGTAAGGTTGATGGCTGAAGCCTTTCTTGCCCAGCTTTCCCCTCCTAATCGTTCACTGAGTTT
CAGTGACAGTGCCATTGCGACCATGCGCCATTACGACTGGCCAGGTAATGTCAGAGAGCTGCAAAACCGCATACGGCGAG
CAGTGATTATGGCAGAAACATCACTTATCACACCCGACGATTTAGGCCTCAGCGTTGCCATTCACGCCAAACACCGCGAC
CTGGTCAGTCAGCGCGATGAACTGGACGTGGAAGCGGTTCTCAAAGCAATCAGCGACCATAAACACAATATGTCTGCCGC
CGCGAGGGCGTTGAATATTTCACGTTCCACCCTCTATCGATTACTGAAAAAGGAACCCGGTTAA

Upstream 100 bases:

>100_bases
CTTGACAACTCTGGCCAAAAACATCGCACCCGCTTCCTTGAGAGACCGCCAATCCTTGCATACACTCAGGATCCGGCGTT
GTGATGGAAATGCAACCGAA

Downstream 100 bases:

>100_bases
GTGTGTCAGTGTCGCAGGTAAATGAAACAACCTGCTTCATGCATGCAACAGCTTTACCCACCATCCAATAAATTTCCCAT
AAAAAACAAATAGATAATTT

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNKVVQKETRAAGTMPRHLLLLSKSQEVTDVSRRLAAYGWHCLTAASGHEAILKLSGQQLGVAVAMLCPRADTLSSDIAL
IQSHFPHLLWIALTDGKLDKACQWLLSARFIDYHHSPLDWDSVNKALGHADGMAKLLPVKTAGVKITDILGTHPCIIRLK
QDIQKLASTDETVLLSGETGTGKGLCAKWLHRMSARADGPFIALNCGALPNGLIQSTLFGHEKGAFTGADRQYQGHIEQA
KDGVLFLDEIADLPLELQVNLLHFLDDRQITRIGGKSPLKINCRIIFATHQNLEKAVDDGTFREDLYHRINVLRLHVPSL
REYCTEVRLMAEAFLAQLSPPNRSLSFSDSAIATMRHYDWPGNVRELQNRIRRAVIMAETSLITPDDLGLSVAIHAKHRD
LVSQRDELDVEAVLKAISDHKHNMSAAARALNISRSTLYRLLKKEPG

Sequences:

>Translated_447_residues
MNKVVQKETRAAGTMPRHLLLLSKSQEVTDVSRRLAAYGWHCLTAASGHEAILKLSGQQLGVAVAMLCPRADTLSSDIAL
IQSHFPHLLWIALTDGKLDKACQWLLSARFIDYHHSPLDWDSVNKALGHADGMAKLLPVKTAGVKITDILGTHPCIIRLK
QDIQKLASTDETVLLSGETGTGKGLCAKWLHRMSARADGPFIALNCGALPNGLIQSTLFGHEKGAFTGADRQYQGHIEQA
KDGVLFLDEIADLPLELQVNLLHFLDDRQITRIGGKSPLKINCRIIFATHQNLEKAVDDGTFREDLYHRINVLRLHVPSL
REYCTEVRLMAEAFLAQLSPPNRSLSFSDSAIATMRHYDWPGNVRELQNRIRRAVIMAETSLITPDDLGLSVAIHAKHRD
LVSQRDELDVEAVLKAISDHKHNMSAAARALNISRSTLYRLLKKEPG
>Mature_447_residues
MNKVVQKETRAAGTMPRHLLLLSKSQEVTDVSRRLAAYGWHCLTAASGHEAILKLSGQQLGVAVAMLCPRADTLSSDIAL
IQSHFPHLLWIALTDGKLDKACQWLLSARFIDYHHSPLDWDSVNKALGHADGMAKLLPVKTAGVKITDILGTHPCIIRLK
QDIQKLASTDETVLLSGETGTGKGLCAKWLHRMSARADGPFIALNCGALPNGLIQSTLFGHEKGAFTGADRQYQGHIEQA
KDGVLFLDEIADLPLELQVNLLHFLDDRQITRIGGKSPLKINCRIIFATHQNLEKAVDDGTFREDLYHRINVLRLHVPSL
REYCTEVRLMAEAFLAQLSPPNRSLSFSDSAIATMRHYDWPGNVRELQNRIRRAVIMAETSLITPDDLGLSVAIHAKHRD
LVSQRDELDVEAVLKAISDHKHNMSAAARALNISRSTLYRLLKKEPG

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=454, Percent_Identity=37.2246696035242, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1790437, Length=425, Percent_Identity=34.8235294117647, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788550, Length=381, Percent_Identity=36.4829396325459, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788905, Length=438, Percent_Identity=34.0182648401827, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=39.0092879256966, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1786524, Length=317, Percent_Identity=39.4321766561514, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87082117, Length=239, Percent_Identity=43.5146443514644, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789087, Length=307, Percent_Identity=35.8306188925081, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=35.7366771159875, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=34.8765432098765, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787583, Length=249, Percent_Identity=36.5461847389558, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789828, Length=320, Percent_Identity=30.3125, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=27.0903010033445, Blast_Score=104, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49537; Mature: 49537

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVVQKETRAAGTMPRHLLLLSKSQEVTDVSRRLAAYGWHCLTAASGHEAILKLSGQQL
CCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHEECCCCCCEEEEECCCHH
GVAVAMLCPRADTLSSDIALIQSHFPHLLWIALTDGKLDKACQWLLSARFIDYHHSPLDW
HHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCH
DSVNKALGHADGMAKLLPVKTAGVKITDILGTHPCIIRLKQDIQKLASTDETVLLSGETG
HHHHHHHHCCCCCHHHEEEEECCEEEEHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
TGKGLCAKWLHRMSARADGPFIALNCGALPNGLIQSTLFGHEKGAFTGADRQYQGHIEQA
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHCCHHHH
KDGVLFLDEIADLPLELQVNLLHFLDDRQITRIGGKSPLKINCRIIFATHQNLEKAVDDG
CCCEEEEHHHHCCCCEEEHHHHHHHCCCCEEECCCCCCEEEEEEEEEEECHHHHHHHCCC
TFREDLYHRINVLRLHVPSLREYCTEVRLMAEAFLAQLSPPNRSLSFSDSAIATMRHYDW
HHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC
PGNVRELQNRIRRAVIMAETSLITPDDLGLSVAIHAKHRDLVSQRDELDVEAVLKAISDH
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHC
KHNMSAAARALNISRSTLYRLLKKEPG
CCCHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNKVVQKETRAAGTMPRHLLLLSKSQEVTDVSRRLAAYGWHCLTAASGHEAILKLSGQQL
CCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHEECCCCCCEEEEECCCHH
GVAVAMLCPRADTLSSDIALIQSHFPHLLWIALTDGKLDKACQWLLSARFIDYHHSPLDW
HHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCH
DSVNKALGHADGMAKLLPVKTAGVKITDILGTHPCIIRLKQDIQKLASTDETVLLSGETG
HHHHHHHHCCCCCHHHEEEEECCEEEEHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
TGKGLCAKWLHRMSARADGPFIALNCGALPNGLIQSTLFGHEKGAFTGADRQYQGHIEQA
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHCCHHHH
KDGVLFLDEIADLPLELQVNLLHFLDDRQITRIGGKSPLKINCRIIFATHQNLEKAVDDG
CCCEEEEHHHHCCCCEEEHHHHHHHCCCCEEECCCCCCEEEEEEEEEEECHHHHHHHCCC
TFREDLYHRINVLRLHVPSLREYCTEVRLMAEAFLAQLSPPNRSLSFSDSAIATMRHYDW
HHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC
PGNVRELQNRIRRAVIMAETSLITPDDLGLSVAIHAKHRDLVSQRDELDVEAVLKAISDH
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHC
KHNMSAAARALNISRSTLYRLLKKEPG
CCCHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]