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The map label for this gene is pspF [H]

Identifier: 119774373

GI number: 119774373

Start: 1520960

End: 1522033

Strand: Reverse

Name: pspF [H]

Synonym: Sama_1236

Alternate gene names: 119774373

Gene position: 1522033-1520960 (Counterclockwise)

Preceding gene: 119774381

Following gene: 119774372

Centisome position: 35.35

GC content: 54.38

Gene sequence:

>1074_bases
GTGCCCAACGCATTTAAACAGGATAACCTCATAGGACAGTCCAATGCACTACTGGAAGTGCTTGAACATGTCTCACGAAT
CGCCCCCCTCTCCAAGCCTGTGCTGATTATTGGGGAACGAGGTACCGGCAAGGAACTGATCGCCGAGCGTCTGCACTATC
TATCCCAGCGCTGGGATCAGAGCTTTCTTAAACTAAATTGTTCATCTTTGAGCGAAAATCTACTCGAAAGTGAACTCTTT
GGCCATGAATCCGGTGCCTTTACCGGCGCCAAGGGGCGTCACGAAGGCCGCTTCGAGCGTGCCGATGGCGGCACCCTGTT
TTTGGATGAGCTGGCCAACACCTCAGGGCTTATCCAGGAAAAGCTGCTCAGAGTTATCGAATATGGCGAGTATGAACGGG
TCGGCGGCAGTAAAACGCTGCAAGCCGATGTCCGACTGATCTGCGCCGCCAACGAAGACTTACCCTCACTGGCTGAAGCG
GGCGAGTTTCGCGCCGACCTGCTCGACAGACTCGCCTTTGACGTCATCACCCTTCCGCCCCTGAGAAGCCGTAAAGAAGA
TATCATGCCGCTGGCTGAATATTTCGGTGTTGGCATGGCGCGGCAACTGAAACTCGAATACTTTCCGGGGTTTGCCCCAC
ACGCCATTGAGCAGCTGCTGAGCCATCAGTGGCCCGGTAACATCCGGGAGCTCAAGAACGTCATAGAACGCTCTGTGTAT
CGCAGCGGTGCCGATGGCGAGCCCATTGAACACATAGTGCTTGACCCCTTCGATTCCCCATGGCGGCCAAAAGGCCGGAT
TAAAACCCGTGAGCGCCAGGTTCAGCCAGCGCTGGCGCCGGAGCCAAAGTCTGAGCCCACATTCGCAACAACTGCTGCAG
TCAGTGAATGTGCGCCAACACCAAGCTTTCCGCTGGATTTGAAAGAACACCTGGAGGCCGTAGAAAAAGACCTCATCCAA
CAGGCGCTGGCAGCAAGTCAGTTCAATCAAAAAAAATCGGCGGACATGTTGGGGCTCAGCTATCACCAGCTGCGCGGGAT
CCTGAAGAAATACAATTTACTGGATAAGGCATAA

Upstream 100 bases:

>100_bases
TTTCAACAACAAAGCTGAAATTTTAAGATTCTCTTTATTTTATAAGTGCAGACACCTATTATGAATTTCACCACTTGTTA
GCGAATAAGACCAACAAAAA

Downstream 100 bases:

>100_bases
GTGGATTGCCGGGGGCGGCGAGCGGCTGTTAGAATCAACCATTACCGCTTACATCAATAACGATTCTTTAATGAATGCGC
TGACAACCCGGCTTTATCGG

Product: psp operon transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPNAFKQDNLIGQSNALLEVLEHVSRIAPLSKPVLIIGERGTGKELIAERLHYLSQRWDQSFLKLNCSSLSENLLESELF
GHESGAFTGAKGRHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEYERVGGSKTLQADVRLICAANEDLPSLAEA
GEFRADLLDRLAFDVITLPPLRSRKEDIMPLAEYFGVGMARQLKLEYFPGFAPHAIEQLLSHQWPGNIRELKNVIERSVY
RSGADGEPIEHIVLDPFDSPWRPKGRIKTRERQVQPALAPEPKSEPTFATTAAVSECAPTPSFPLDLKEHLEAVEKDLIQ
QALAASQFNQKKSADMLGLSYHQLRGILKKYNLLDKA

Sequences:

>Translated_357_residues
MPNAFKQDNLIGQSNALLEVLEHVSRIAPLSKPVLIIGERGTGKELIAERLHYLSQRWDQSFLKLNCSSLSENLLESELF
GHESGAFTGAKGRHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEYERVGGSKTLQADVRLICAANEDLPSLAEA
GEFRADLLDRLAFDVITLPPLRSRKEDIMPLAEYFGVGMARQLKLEYFPGFAPHAIEQLLSHQWPGNIRELKNVIERSVY
RSGADGEPIEHIVLDPFDSPWRPKGRIKTRERQVQPALAPEPKSEPTFATTAAVSECAPTPSFPLDLKEHLEAVEKDLIQ
QALAASQFNQKKSADMLGLSYHQLRGILKKYNLLDKA
>Mature_356_residues
PNAFKQDNLIGQSNALLEVLEHVSRIAPLSKPVLIIGERGTGKELIAERLHYLSQRWDQSFLKLNCSSLSENLLESELFG
HESGAFTGAKGRHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEYERVGGSKTLQADVRLICAANEDLPSLAEAG
EFRADLLDRLAFDVITLPPLRSRKEDIMPLAEYFGVGMARQLKLEYFPGFAPHAIEQLLSHQWPGNIRELKNVIERSVYR
SGADGEPIEHIVLDPFDSPWRPKGRIKTRERQVQPALAPEPKSEPTFATTAAVSECAPTPSFPLDLKEHLEAVEKDLIQQ
ALAASQFNQKKSADMLGLSYHQLRGILKKYNLLDKA

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=347, Percent_Identity=59.0778097982709, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI1788550, Length=353, Percent_Identity=38.2436260623229, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=50.2164502164502, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1790437, Length=233, Percent_Identity=48.068669527897, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI1789087, Length=232, Percent_Identity=48.7068965517241, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789233, Length=352, Percent_Identity=39.7727272727273, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI87082152, Length=357, Percent_Identity=37.2549019607843, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1790299, Length=346, Percent_Identity=36.1271676300578, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1788905, Length=230, Percent_Identity=41.7391304347826, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=42.741935483871, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1787583, Length=258, Percent_Identity=39.922480620155, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=33.7719298245614, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081858, Length=217, Percent_Identity=32.7188940092166, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 39878; Mature: 39747

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNAFKQDNLIGQSNALLEVLEHVSRIAPLSKPVLIIGERGTGKELIAERLHYLSQRWDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SFLKLNCSSLSENLLESELFGHESGAFTGAKGRHEGRFERADGGTLFLDELANTSGLIQE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEYERVGGSKTLQADVRLICAANEDLPSLAEAGEFRADLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCCCHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
LRSRKEDIMPLAEYFGVGMARQLKLEYFPGFAPHAIEQLLSHQWPGNIRELKNVIERSVY
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RSGADGEPIEHIVLDPFDSPWRPKGRIKTRERQVQPALAPEPKSEPTFATTAAVSECAPT
HCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCC
PSFPLDLKEHLEAVEKDLIQQALAASQFNQKKSADMLGLSYHQLRGILKKYNLLDKA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNAFKQDNLIGQSNALLEVLEHVSRIAPLSKPVLIIGERGTGKELIAERLHYLSQRWDQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SFLKLNCSSLSENLLESELFGHESGAFTGAKGRHEGRFERADGGTLFLDELANTSGLIQE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEYERVGGSKTLQADVRLICAANEDLPSLAEAGEFRADLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCCCHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
LRSRKEDIMPLAEYFGVGMARQLKLEYFPGFAPHAIEQLLSHQWPGNIRELKNVIERSVY
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RSGADGEPIEHIVLDPFDSPWRPKGRIKTRERQVQPALAPEPKSEPTFATTAAVSECAPT
HCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCC
PSFPLDLKEHLEAVEKDLIQQALAASQFNQKKSADMLGLSYHQLRGILKKYNLLDKA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]