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The map label for this gene is kefB [H]

Identifier: 119774296

GI number: 119774296

Start: 1412896

End: 1414842

Strand: Direct

Name: kefB [H]

Synonym: Sama_1159

Alternate gene names: 119774296

Gene position: 1412896-1414842 (Clockwise)

Preceding gene: 119774294

Following gene: 119774306

Centisome position: 32.81

GC content: 56.91

Gene sequence:

>1947_bases
ATGGAACAGGGCATACTTCTCAAGATCTTGTTGATGCTGCTTATCGCCATTGCAGCCATCGCGCTCTTGAGACGTTTGGG
TTTGCCCGCCATTCTCGCTTACCTTCTCACCGGTGTGGTTGCCGGTCCCGGCGGATTTGACTGGTTCAGTCAGCACCAGA
TGCATTCGGTGGCCGAACTTGGGGTCGTCCTCCTGATGTTTACCTTGGGGCTGGAGTTTTCTGTCCCCAAACTCTGGGCC
ATGCGCCGTACCGTGTTTGGCCTGGGCAGCGCTCAGGTGCTGGTCACGACTCTTCTCACCATGTTGATTGCCATGGCCCT
TGGTCAGCAGCCGGTACAGGCGCTGGTGATTGGCTCAACTATCGCGCTTTCTTCCACCGCCATAGTGCTTAAGCTCCTGA
ATGAACAGGGATGGTTACGTCGGCGCCACGGTGAGTTATCCGTGAGTGTGCTGCTGTTTCAGGATTTGGCTGTTGTGCCA
TTACTTATCCTGCTCCCCTTGCTTGGTGAGGCCGACGGGACGCTTGCCCTCCACAATGTGATGTTTGCTTTGCTCAAAGG
CACAGTGGCCTTTATTGCCCTGATGGCGTTTGGTAAGTGGGCACTTCCAAAGGTGTTCGATGAGGTGGCGCGTTCCCGCT
CCAATGAACTCTTTGTGCTCTCAGCGCTGGTGGTGGCGCTGCTGACCGGTGCCTTTACCCAATGGCTAGGACTTTCCATG
GCACTGGGGGCCTTTATGGCCGGCATGTTGCTGGGAGAAAGTCAGTATCGGCGACAGCTTGAAGCCGATATTCGGCCGTT
CCGGGATCTCTTGATGGGGCTCTTTTTTATCTCCATCGGCATGTTGCTGGATTTCCGGTTAGTGATGGCTTACTGGTGGC
AGATCTTACTGGTGCTGCTGGCGGTGATCAGTGGCAAGGCGCTGGTGGTTCATGGGCTCTTGAAGCTTGCAGGTGAGCCA
TTTCGTATCTCCTCGGTGGCGGCTCTCATTTTGGCGCAGGTGGGGGAGTTTTCCTTCGTGGTGCTGGCGCTGGCAGTGAA
TTATCAGCTGCTGGAGCCACAGGTCAGCACTGTGTTGGTGATGGTGGCGGTGATCTCCATGGCTCTGGCGCCAACCCTGG
TGCGTCATGCGGTAAGTATCAGCAAATGGCTACAGGGGTTAAAACCTTCGGTTAAAGACGACCCCCATCTTCCCAAGGTG
GAAGAGGGGACCGAGCTGGTGCTTATCCTGGGTTATGGTCGTGTCGGCCAAACCATCGCCCGCTTCCTGAAGGCCGAAGC
CGTGCCATTTTTGGCGCTGGATCTTGACCCCACCCGGGTGCAGGAAGCCAAGCGTGCCGGCGAGCCAGTGTATTTTGGTG
ATGTCTGTAAGCGTGCCATTTTAAAGCAGGCGGGGATCAGTCAGGCAAAGATGATAGTGCTGACTTTCTGCGACAGCCGT
GCTGTGGATGAGGTGATCCACCTGTGCCGAACTCTGGCGCCAACGGCGAAGATTCTGGTTCGCACCCGGGATGACAGCGA
ACTCAAATTGCTGGAGCAAGCCGGTGCCAGTCAGGTGATCCCTGAGACCCTCGAAGGCAGCCTGATGCTGGTCAGTCAGG
TGTTGTATCAGTGCGGTGTTCCCTTAACCCGGATTTTAAAGCGGCTCGAGTTTGAGCGGCGCAATCATTATCAGTACCTG
CATGGCTTTTTCTCCGGCACAGAAACCGACTTTACCCTGGAGCTCTTGCATGCAGTGGCGCTGCCAAGGGGCGCCAGTGT
GGTAGGGCAACGACTCGGCGACATTCCCTGGGCCAAACTCAGGGTCGAGCTCAGGGCCATTCGCCGAGGGGGCGAGGAGT
TCGACAGCCCGAGCGAAGACTGGCAGCTGCGCGCCGGTGACGTGTTAATTTTGCTTGGTAAACCCAGGCGGGTGGAAAAA
GCCGAGGCGCTGCTGCTTCAGGGCTGA

Upstream 100 bases:

>100_bases
GAAAAAGCCGTTAAATAAATTTGTCTTTGTATGTCACTCAGTTAATCTTTGTTATGGCAGTGCAAATTTTCCCGTGCCGG
TAATTTGACAAGGAAACTTC

Downstream 100 bases:

>100_bases
TGTCGGGTACTGCCCAAAGCGCCCATCACCAGCGCTTATTGGCACTGAATCGATGGCGGTCAGTCGATATTAAAAAATGC
TTTTATTTCATCGATTTGGC

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MEQGILLKILLMLLIAIAAIALLRRLGLPAILAYLLTGVVAGPGGFDWFSQHQMHSVAELGVVLLMFTLGLEFSVPKLWA
MRRTVFGLGSAQVLVTTLLTMLIAMALGQQPVQALVIGSTIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLGEADGTLALHNVMFALLKGTVAFIALMAFGKWALPKVFDEVARSRSNELFVLSALVVALLTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVMAYWWQILLVLLAVISGKALVVHGLLKLAGEP
FRISSVAALILAQVGEFSFVVLALAVNYQLLEPQVSTVLVMVAVISMALAPTLVRHAVSISKWLQGLKPSVKDDPHLPKV
EEGTELVLILGYGRVGQTIARFLKAEAVPFLALDLDPTRVQEAKRAGEPVYFGDVCKRAILKQAGISQAKMIVLTFCDSR
AVDEVIHLCRTLAPTAKILVRTRDDSELKLLEQAGASQVIPETLEGSLMLVSQVLYQCGVPLTRILKRLEFERRNHYQYL
HGFFSGTETDFTLELLHAVALPRGASVVGQRLGDIPWAKLRVELRAIRRGGEEFDSPSEDWQLRAGDVLILLGKPRRVEK
AEALLLQG

Sequences:

>Translated_648_residues
MEQGILLKILLMLLIAIAAIALLRRLGLPAILAYLLTGVVAGPGGFDWFSQHQMHSVAELGVVLLMFTLGLEFSVPKLWA
MRRTVFGLGSAQVLVTTLLTMLIAMALGQQPVQALVIGSTIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLGEADGTLALHNVMFALLKGTVAFIALMAFGKWALPKVFDEVARSRSNELFVLSALVVALLTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVMAYWWQILLVLLAVISGKALVVHGLLKLAGEP
FRISSVAALILAQVGEFSFVVLALAVNYQLLEPQVSTVLVMVAVISMALAPTLVRHAVSISKWLQGLKPSVKDDPHLPKV
EEGTELVLILGYGRVGQTIARFLKAEAVPFLALDLDPTRVQEAKRAGEPVYFGDVCKRAILKQAGISQAKMIVLTFCDSR
AVDEVIHLCRTLAPTAKILVRTRDDSELKLLEQAGASQVIPETLEGSLMLVSQVLYQCGVPLTRILKRLEFERRNHYQYL
HGFFSGTETDFTLELLHAVALPRGASVVGQRLGDIPWAKLRVELRAIRRGGEEFDSPSEDWQLRAGDVLILLGKPRRVEK
AEALLLQG
>Mature_648_residues
MEQGILLKILLMLLIAIAAIALLRRLGLPAILAYLLTGVVAGPGGFDWFSQHQMHSVAELGVVLLMFTLGLEFSVPKLWA
MRRTVFGLGSAQVLVTTLLTMLIAMALGQQPVQALVIGSTIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLGEADGTLALHNVMFALLKGTVAFIALMAFGKWALPKVFDEVARSRSNELFVLSALVVALLTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVMAYWWQILLVLLAVISGKALVVHGLLKLAGEP
FRISSVAALILAQVGEFSFVVLALAVNYQLLEPQVSTVLVMVAVISMALAPTLVRHAVSISKWLQGLKPSVKDDPHLPKV
EEGTELVLILGYGRVGQTIARFLKAEAVPFLALDLDPTRVQEAKRAGEPVYFGDVCKRAILKQAGISQAKMIVLTFCDSR
AVDEVIHLCRTLAPTAKILVRTRDDSELKLLEQAGASQVIPETLEGSLMLVSQVLYQCGVPLTRILKRLEFERRNHYQYL
HGFFSGTETDFTLELLHAVALPRGASVVGQRLGDIPWAKLRVELRAIRRGGEEFDSPSEDWQLRAGDVLILLGKPRRVEK
AEALLLQG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=387, Percent_Identity=29.1989664082687, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1789749, Length=552, Percent_Identity=36.4130434782609, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1786232, Length=564, Percent_Identity=32.4468085106383, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI1786685, Length=516, Percent_Identity=31.3953488372093, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6322367, Length=532, Percent_Identity=20.6766917293233, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71045; Mature: 71045

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQGILLKILLMLLIAIAAIALLRRLGLPAILAYLLTGVVAGPGGFDWFSQHQMHSVAEL
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GVVLLMFTLGLEFSVPKLWAMRRTVFGLGSAQVLVTTLLTMLIAMALGQQPVQALVIGST
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVPLLILLPLLGEADGTLALHNV
HHHHHHHHHHHHHHCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
MFALLKGTVAFIALMAFGKWALPKVFDEVARSRSNELFVLSALVVALLTGAFTQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVMAYWWQILLVLL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVISGKALVVHGLLKLAGEPFRISSVAALILAQVGEFSFVVLALAVNYQLLEPQVSTVLV
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHH
MVAVISMALAPTLVRHAVSISKWLQGLKPSVKDDPHLPKVEEGTELVLILGYGRVGQTIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
RFLKAEAVPFLALDLDPTRVQEAKRAGEPVYFGDVCKRAILKQAGISQAKMIVLTFCDSR
HHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEECCCH
AVDEVIHLCRTLAPTAKILVRTRDDSELKLLEQAGASQVIPETLEGSLMLVSQVLYQCGV
HHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCCC
PLTRILKRLEFERRNHYQYLHGFFSGTETDFTLELLHAVALPRGASVVGQRLGDIPWAKL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
RVELRAIRRGGEEFDSPSEDWQLRAGDVLILLGKPRRVEKAEALLLQG
HHHHHHHHCCCHHCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEQGILLKILLMLLIAIAAIALLRRLGLPAILAYLLTGVVAGPGGFDWFSQHQMHSVAEL
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GVVLLMFTLGLEFSVPKLWAMRRTVFGLGSAQVLVTTLLTMLIAMALGQQPVQALVIGST
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVPLLILLPLLGEADGTLALHNV
HHHHHHHHHHHHHHCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
MFALLKGTVAFIALMAFGKWALPKVFDEVARSRSNELFVLSALVVALLTGAFTQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFRLVMAYWWQILLVLL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVISGKALVVHGLLKLAGEPFRISSVAALILAQVGEFSFVVLALAVNYQLLEPQVSTVLV
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHH
MVAVISMALAPTLVRHAVSISKWLQGLKPSVKDDPHLPKVEEGTELVLILGYGRVGQTIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
RFLKAEAVPFLALDLDPTRVQEAKRAGEPVYFGDVCKRAILKQAGISQAKMIVLTFCDSR
HHHHHHCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEECCCH
AVDEVIHLCRTLAPTAKILVRTRDDSELKLLEQAGASQVIPETLEGSLMLVSQVLYQCGV
HHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCCC
PLTRILKRLEFERRNHYQYLHGFFSGTETDFTLELLHAVALPRGASVVGQRLGDIPWAKL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
RVELRAIRRGGEEFDSPSEDWQLRAGDVLILLGKPRRVEKAEALLLQG
HHHHHHHHCCCHHCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA