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The map label for this gene is tilS [H]

Identifier: 119774292

GI number: 119774292

Start: 1407601

End: 1409094

Strand: Direct

Name: tilS [H]

Synonym: Sama_1155

Alternate gene names: 119774292

Gene position: 1407601-1409094 (Clockwise)

Preceding gene: 119774291

Following gene: 119774294

Centisome position: 32.69

GC content: 58.23

Gene sequence:

>1494_bases
ATGCAAACCTCGCCAGCGGCCGTTGCAACGCTTGATTCACTCGTCTTGCTGATTGAGGCGGCACTTGCAGGCCTGACGCC
TTCCAAACTGGTGCTGGGTTACAGCGGCGGGCTGGACTCAGAGCTTATGGCCTGCGCCCTGTCTCATTACGCAAAAAAGC
ATCCTGGCACCCAGTGTCTGCTGGTGCATGTACATCATGGCCTTAGCCCGAATGCCGATACCTGGGCCCGTCATTGCGAG
CATCGTGCCGCTCGCTACGAACTGGAGTTTGTGCTGGAAAAGGTCAGGGTAAAGCAGGGTGCCCGTCTAAGTCTGGAGGC
CGAGGCTCGCAGCGCCAGATATGCTGCCTTAATGGCCCATTTGGCCGAAGGCGACGTCCTCCTTACCGCCCATCATCAGG
ACGACCAGCTGGAAACCGTGCTTCTGGCGCTGGCCCGGGGATTGGGCCCCAAGGGGCTTGCGGCCATGGGACAACAACAG
TCCCTCGATGACGGAGCATTCCAGGTAAGGCCTTTTCTGGGCTTGTCCCGTGAGTATCTTGAAGACGCTGCGTCGGCGCT
TGATCTCAGCCACATCGAAGATGAAAGCAATCAGGACACCCGCTTCGACCGCAACTTTCTGCGAGCCGAAATTATTCCGC
GACTCAAATCCCGCTGGGGTGCCATAGGCGCGACTGCGAGTCGCAGTGCTGAGCTGTGCGCCCAAACCCAAATGCTACTG
GATGATGAGGTCAGTGAGCGCCTGACTCCCCTTATCAGCCGTTGTCCTCTGAGTCATGCGTTCAGACTGGCACTGGGGCC
AGTCGCTGCCAGCTCAAGGGCGTGGCAGGCACAATTGGTTCGGGGTTTTCTGGAGGTTGCCGGTCTGCCTCCACCATCAA
AAATTCAGCTCGACGAGGCATTAAGCCAGCTTTTCGCCGCCAAAGCGGATGCCGCGGTTTCGCTGCGTTTTGGTCGCACC
CGTTTGAGGCGATATCAGGGCTGGCTCTACGCCGAAACTGAATCTGATAACGCCAGTCATTCACTGTCCGGTTCCACTAA
AACGTCGGCCACTAAAACGTCGGCCACTCAAACGTCGGCGACCCAAACGCCTTTCAGGCAAACTCCTGTCACGCAGCAGA
TGCTGGCTGAAGGGATTGCGACGCCTTTGGGCAAGCTGCAACTGCTGCCAACAGACGCGGAGAATGAAGGGCAGGACGCG
CATTATTGGCAAGCCAGAGTGCCCCTTGGTGCACTCATAGGCGACCTGACGCTGGTATATGGTTTACCCGGCAGCACCAA
GGCCTGGCCGGTGGGCCGTGGTAAGCAGCGGGAATTGAAGAAGCTCTGGCAGGAGTTTGACGTACCGCCCTGGCTTAGAA
TATCCATCCCCATGCTCTGCTGCGACGAGGCGCTGGTAGCCGCTGCCGGAGTATGGGTTGAAAAGACGGCGCTGGAGAAG
TCATCGCAACAAGAGAGTGAAGACTGGCTGACCATCAGGCTTTTGCGCATCTGA

Upstream 100 bases:

>100_bases
GGTGACGACTGGCGGGTTGACCCGTCTGACGACCTGGTACTGGCACTGCAAACCCTGATAGGCCCCGATAAAATTCGGAT
CCAGTTCTAGGATGAGTGGC

Downstream 100 bases:

>100_bases
CTATTCTTATTACAGCGAATATCTCCTTCTTGAAACCTCACACACTGACACCAAACGGTGTTCAGTGATGGTTTTCCGGA
TAAAGCACCACGCAGGCGGT

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MQTSPAAVATLDSLVLLIEAALAGLTPSKLVLGYSGGLDSELMACALSHYAKKHPGTQCLLVHVHHGLSPNADTWARHCE
HRAARYELEFVLEKVRVKQGARLSLEAEARSARYAALMAHLAEGDVLLTAHHQDDQLETVLLALARGLGPKGLAAMGQQQ
SLDDGAFQVRPFLGLSREYLEDAASALDLSHIEDESNQDTRFDRNFLRAEIIPRLKSRWGAIGATASRSAELCAQTQMLL
DDEVSERLTPLISRCPLSHAFRLALGPVAASSRAWQAQLVRGFLEVAGLPPPSKIQLDEALSQLFAAKADAAVSLRFGRT
RLRRYQGWLYAETESDNASHSLSGSTKTSATKTSATQTSATQTPFRQTPVTQQMLAEGIATPLGKLQLLPTDAENEGQDA
HYWQARVPLGALIGDLTLVYGLPGSTKAWPVGRGKQRELKKLWQEFDVPPWLRISIPMLCCDEALVAAAGVWVEKTALEK
SSQQESEDWLTIRLLRI

Sequences:

>Translated_497_residues
MQTSPAAVATLDSLVLLIEAALAGLTPSKLVLGYSGGLDSELMACALSHYAKKHPGTQCLLVHVHHGLSPNADTWARHCE
HRAARYELEFVLEKVRVKQGARLSLEAEARSARYAALMAHLAEGDVLLTAHHQDDQLETVLLALARGLGPKGLAAMGQQQ
SLDDGAFQVRPFLGLSREYLEDAASALDLSHIEDESNQDTRFDRNFLRAEIIPRLKSRWGAIGATASRSAELCAQTQMLL
DDEVSERLTPLISRCPLSHAFRLALGPVAASSRAWQAQLVRGFLEVAGLPPPSKIQLDEALSQLFAAKADAAVSLRFGRT
RLRRYQGWLYAETESDNASHSLSGSTKTSATKTSATQTSATQTPFRQTPVTQQMLAEGIATPLGKLQLLPTDAENEGQDA
HYWQARVPLGALIGDLTLVYGLPGSTKAWPVGRGKQRELKKLWQEFDVPPWLRISIPMLCCDEALVAAAGVWVEKTALEK
SSQQESEDWLTIRLLRI
>Mature_497_residues
MQTSPAAVATLDSLVLLIEAALAGLTPSKLVLGYSGGLDSELMACALSHYAKKHPGTQCLLVHVHHGLSPNADTWARHCE
HRAARYELEFVLEKVRVKQGARLSLEAEARSARYAALMAHLAEGDVLLTAHHQDDQLETVLLALARGLGPKGLAAMGQQQ
SLDDGAFQVRPFLGLSREYLEDAASALDLSHIEDESNQDTRFDRNFLRAEIIPRLKSRWGAIGATASRSAELCAQTQMLL
DDEVSERLTPLISRCPLSHAFRLALGPVAASSRAWQAQLVRGFLEVAGLPPPSKIQLDEALSQLFAAKADAAVSLRFGRT
RLRRYQGWLYAETESDNASHSLSGSTKTSATKTSATQTSATQTPFRQTPVTQQMLAEGIATPLGKLQLLPTDAENEGQDA
HYWQARVPLGALIGDLTLVYGLPGSTKAWPVGRGKQRELKKLWQEFDVPPWLRISIPMLCCDEALVAAAGVWVEKTALEK
SSQQESEDWLTIRLLRI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=465, Percent_Identity=36.3440860215054, Blast_Score=235, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54272; Mature: 54272

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSPAAVATLDSLVLLIEAALAGLTPSKLVLGYSGGLDSELMACALSHYAKKHPGTQCL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
LVHVHHGLSPNADTWARHCEHRAARYELEFVLEKVRVKQGARLSLEAEARSARYAALMAH
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
LAEGDVLLTAHHQDDQLETVLLALARGLGPKGLAAMGQQQSLDDGAFQVRPFLGLSREYL
HCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEECHHHCCCHHHH
EDAASALDLSHIEDESNQDTRFDRNFLRAEIIPRLKSRWGAIGATASRSAELCAQTQMLL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DDEVSERLTPLISRCPLSHAFRLALGPVAASSRAWQAQLVRGFLEVAGLPPPSKIQLDEA
HHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHH
LSQLFAAKADAAVSLRFGRTRLRRYQGWLYAETESDNASHSLSGSTKTSATKTSATQTSA
HHHHHHHCCCCEEEEHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQTPFRQTPVTQQMLAEGIATPLGKLQLLPTDAENEGQDAHYWQARVPLGALIGDLTLVY
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHEE
GLPGSTKAWPVGRGKQRELKKLWQEFDVPPWLRISIPMLCCDEALVAAAGVWVEKTALEK
ECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
SSQQESEDWLTIRLLRI
HHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MQTSPAAVATLDSLVLLIEAALAGLTPSKLVLGYSGGLDSELMACALSHYAKKHPGTQCL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
LVHVHHGLSPNADTWARHCEHRAARYELEFVLEKVRVKQGARLSLEAEARSARYAALMAH
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
LAEGDVLLTAHHQDDQLETVLLALARGLGPKGLAAMGQQQSLDDGAFQVRPFLGLSREYL
HCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCEEECHHHCCCHHHH
EDAASALDLSHIEDESNQDTRFDRNFLRAEIIPRLKSRWGAIGATASRSAELCAQTQMLL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DDEVSERLTPLISRCPLSHAFRLALGPVAASSRAWQAQLVRGFLEVAGLPPPSKIQLDEA
HHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHH
LSQLFAAKADAAVSLRFGRTRLRRYQGWLYAETESDNASHSLSGSTKTSATKTSATQTSA
HHHHHHHCCCCEEEEHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQTPFRQTPVTQQMLAEGIATPLGKLQLLPTDAENEGQDAHYWQARVPLGALIGDLTLVY
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHEE
GLPGSTKAWPVGRGKQRELKKLWQEFDVPPWLRISIPMLCCDEALVAAAGVWVEKTALEK
ECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
SSQQESEDWLTIRLLRI
HHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]