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The map label for this gene is pksE [H]

Identifier: 119774249

GI number: 119774249

Start: 1349680

End: 1351293

Strand: Reverse

Name: pksE [H]

Synonym: Sama_1112

Alternate gene names: 119774249

Gene position: 1351293-1349680 (Counterclockwise)

Preceding gene: 119774250

Following gene: 119774247

Centisome position: 31.38

GC content: 60.16

Gene sequence:

>1614_bases
ATGAGCACAGCTTTCAAGTGGCCATGGGCCATAGACCAAGCCTCGGGCATAAGCCATGTGGACGCCTTTAAGTCGGCATT
GCTTGAGCTGAATAAGCCCCTGTATGTGGCGCGCCTGCCCGGTGGTGAGCTTTGCCTGAGCCACGATGCTTCTGAGCAAC
AACCGGAGCAAGGCCGAGAGCCCGGGCTGCTGCCGGTGGCCGCTTTTGCCGCCCCCTTTGCCGCCTGTGAGCTTGGCAGT
GAGGATTTTCGCCGCTGCCACGGGGTAAAATACGCCTACTACGCTGGCGCCATGGCCAATGGCATCGCCTCCGAAGAGCT
GGTAATCGCCCTCGGTAAAGCAGGCATCCTCTGCTCCTTTGGCGCCGCAGGATTGGTGCCAGCCCGTGTTGAAGCCGCCA
TCAAGCGCATTCAGGCCGAGCTGCCACAGGGCCCCTACGCCTTTAACCTCATCCACAGCCCAAGCGAACCTGCGCTGGAG
CAAGGCGCGGTGGAGCTTTACTTAAAGCACGGCGTGCACACGGTAGAAGCCTCAGCCTTTTTGGCGCTGACCCCGCACAT
AGTGCGCTACCGCGCCGCAGGCTTAAGCCGCGACAACGATGGCAATATCGTCATTAAAAACAAGGTGATTGCCAAAATCA
GCCGCACCGAGGTCGCCAGCCACTTTATGAAGAGTGCGCCGAAAAAGATGCTCGATACCCTGGTAGCCGAGGGCCATATC
AGCACTGAGCAGGCCGAGCTTGCCTTGCTGGTGCCCATGGCCGATGACATCACCGCCGAAGCCGACTCCGGCGGCCACAC
CGACAACCGTCCGCTGGTGACTCTGCTGCCCACCATTCTGGCGCTGCGTGACGAGCTGGCAACCAGCCAATCGTTCGCTA
CCCCCATCCGTGTCGGCGCAGGCGGCGGCATAGGCACGCCGGATGCGGCCTTGGCCGCCTTTAATATGGGGGCGGATTTT
ATCGTCACCGGCTCCATCAATCAGGCCTGTATCGAGGCCGGTGCCAGCGAGCACACCCGTAAGCTGCTCGCCACCACCGA
AATGGCCGATGTGACCATGGCACCGGCCGCCGACATGTTCGAGATGGGCGTTAAGCTTCAGGTAGTAAAACGCGGCACTC
TGTTCCCGATGCGCGCCAACAAGCTGTACGAGATTTACAGCCGCTACGACAGCATCGAAGCCATACCGGCAGATGAGCGC
CAAAAGCTCGAAGCCCAGGTGTTTCGCGCCAGCCTCGATGACATTTGGCAAGGCACTGTGGCGCATTTTATGGAGCGCGA
TCCAAGTCAGATTCAGCGCGCCGAGGGTAACCCCAAGCGCAAGATGGCACTGATTTTCCGCTGGTATCTGGGGCTCTCCA
GCCGCTGGTCGAATACCGGCGAAGCAGGCCGCGAGATGGATTATCAAATCTGGGCAGGCCCGGCACTCGGCGCCTTTAAC
GGCTGGTCGAAGGGAACTTACCTTGATGATTACCGCGAGCGCCGCGCAGTGGACTTGGCGCTGCATTTGCTCAGCGGGGC
AGCGTATTTACATCGGGTGAATTCCTTGAAGGCTCAAGGGATTGTGTTGCCTTCTGCGGTGGCGCGGTATCGACCGTTGG
AGCCGTTGGCTTAA

Upstream 100 bases:

>100_bases
AATTGAAGAAGCCTGAAGCGCCACGACAAAACAGCATTGAACGCGGCCAACTGCCGCTGATAAGAAGAATTGCGGTACTG
ACCGCCATAAGAGAGACAAG

Downstream 100 bases:

>100_bases
TGCGTAAGCCAAACTAACGCTCAATGTGCATGTTGCCAGTCGGATAGTCGGTGGTCAGTTGCACTGGACGGTATGAATGA
AAAAACCAGCCATAGCGCTG

Product: omega-3 polyunsaturated fatty acid synthase PfaD

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSTAFKWPWAIDQASGISHVDAFKSALLELNKPLYVARLPGGELCLSHDASEQQPEQGREPGLLPVAAFAAPFAACELGS
EDFRRCHGVKYAYYAGAMANGIASEELVIALGKAGILCSFGAAGLVPARVEAAIKRIQAELPQGPYAFNLIHSPSEPALE
QGAVELYLKHGVHTVEASAFLALTPHIVRYRAAGLSRDNDGNIVIKNKVIAKISRTEVASHFMKSAPKKMLDTLVAEGHI
STEQAELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALRDELATSQSFATPIRVGAGGGIGTPDAALAAFNMGADF
IVTGSINQACIEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYSRYDSIEAIPADER
QKLEAQVFRASLDDIWQGTVAHFMERDPSQIQRAEGNPKRKMALIFRWYLGLSSRWSNTGEAGREMDYQIWAGPALGAFN
GWSKGTYLDDYRERRAVDLALHLLSGAAYLHRVNSLKAQGIVLPSAVARYRPLEPLA

Sequences:

>Translated_537_residues
MSTAFKWPWAIDQASGISHVDAFKSALLELNKPLYVARLPGGELCLSHDASEQQPEQGREPGLLPVAAFAAPFAACELGS
EDFRRCHGVKYAYYAGAMANGIASEELVIALGKAGILCSFGAAGLVPARVEAAIKRIQAELPQGPYAFNLIHSPSEPALE
QGAVELYLKHGVHTVEASAFLALTPHIVRYRAAGLSRDNDGNIVIKNKVIAKISRTEVASHFMKSAPKKMLDTLVAEGHI
STEQAELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALRDELATSQSFATPIRVGAGGGIGTPDAALAAFNMGADF
IVTGSINQACIEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYSRYDSIEAIPADER
QKLEAQVFRASLDDIWQGTVAHFMERDPSQIQRAEGNPKRKMALIFRWYLGLSSRWSNTGEAGREMDYQIWAGPALGAFN
GWSKGTYLDDYRERRAVDLALHLLSGAAYLHRVNSLKAQGIVLPSAVARYRPLEPLA
>Mature_536_residues
STAFKWPWAIDQASGISHVDAFKSALLELNKPLYVARLPGGELCLSHDASEQQPEQGREPGLLPVAAFAAPFAACELGSE
DFRRCHGVKYAYYAGAMANGIASEELVIALGKAGILCSFGAAGLVPARVEAAIKRIQAELPQGPYAFNLIHSPSEPALEQ
GAVELYLKHGVHTVEASAFLALTPHIVRYRAAGLSRDNDGNIVIKNKVIAKISRTEVASHFMKSAPKKMLDTLVAEGHIS
TEQAELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALRDELATSQSFATPIRVGAGGGIGTPDAALAAFNMGADFI
VTGSINQACIEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYSRYDSIEAIPADERQ
KLEAQVFRASLDDIWQGTVAHFMERDPSQIQRAEGNPKRKMALIFRWYLGLSSRWSNTGEAGREMDYQIWAGPALGAFNG
WSKGTYLDDYRERRAVDLALHLLSGAAYLHRVNSLKAQGIVLPSAVARYRPLEPLA

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 58096; Mature: 57965

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAFKWPWAIDQASGISHVDAFKSALLELNKPLYVARLPGGELCLSHDASEQQPEQGRE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHCCC
PGLLPVAAFAAPFAACELGSEDFRRCHGVKYAYYAGAMANGIASEELVIALGKAGILCSF
CCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEC
GAAGLVPARVEAAIKRIQAELPQGPYAFNLIHSPSEPALEQGAVELYLKHGVHTVEASAF
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCEEECCCEE
LALTPHIVRYRAAGLSRDNDGNIVIKNKVIAKISRTEVASHFMKSAPKKMLDTLVAEGHI
EECCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
STEQAELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALRDELATSQSFATPIRVGA
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GGGIGTPDAALAAFNMGADFIVTGSINQACIEAGASEHTRKLLATTEMADVTMAPAADMF
CCCCCCCHHHHHHHCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHEEECCCHHHH
EMGVKLQVVKRGTLFPMRANKLYEIYSRYDSIEAIPADERQKLEAQVFRASLDDIWQGTV
HCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHFMERDPSQIQRAEGNPKRKMALIFRWYLGLSSRWSNTGEAGREMDYQIWAGPALGAFN
HHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GWSKGTYLDDYRERRAVDLALHLLSGAAYLHRVNSLKAQGIVLPSAVARYRPLEPLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHCCCCCCCC
>Mature Secondary Structure 
STAFKWPWAIDQASGISHVDAFKSALLELNKPLYVARLPGGELCLSHDASEQQPEQGRE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCHHCCC
PGLLPVAAFAAPFAACELGSEDFRRCHGVKYAYYAGAMANGIASEELVIALGKAGILCSF
CCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEC
GAAGLVPARVEAAIKRIQAELPQGPYAFNLIHSPSEPALEQGAVELYLKHGVHTVEASAF
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCEEECCCEE
LALTPHIVRYRAAGLSRDNDGNIVIKNKVIAKISRTEVASHFMKSAPKKMLDTLVAEGHI
EECCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
STEQAELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALRDELATSQSFATPIRVGA
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GGGIGTPDAALAAFNMGADFIVTGSINQACIEAGASEHTRKLLATTEMADVTMAPAADMF
CCCCCCCHHHHHHHCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHEEECCCHHHH
EMGVKLQVVKRGTLFPMRANKLYEIYSRYDSIEAIPADERQKLEAQVFRASLDDIWQGTV
HCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHFMERDPSQIQRAEGNPKRKMALIFRWYLGLSSRWSNTGEAGREMDYQIWAGPALGAFN
HHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GWSKGTYLDDYRERRAVDLALHLLSGAAYLHRVNSLKAQGIVLPSAVARYRPLEPLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]