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The map label for this gene is ispD

Identifier: 119774175

GI number: 119774175

Start: 1256902

End: 1257618

Strand: Direct

Name: ispD

Synonym: Sama_1038

Alternate gene names: 119774175

Gene position: 1256902-1257618 (Clockwise)

Preceding gene: 119774174

Following gene: 119774176

Centisome position: 29.19

GC content: 57.88

Gene sequence:

>717_bases
ATGAATTTCCAATCAAGCTCCAGTGGCAGCATCGTCGCTATTGTTCCTGCGGCCGGAATTGGCAGCCGAATGGGGGCCAC
TATCCCGAAACAATACCTGCCGTTATTGGATAAACCTATCCTCGCCCATACCCTGCAAAGGCTGCTCTCCCATCCTGCTA
TTGATAAGGTCATAGTCGCTGTCTCGGCCGAAGATAGCTGGTTTGACAGTTTGGCTGAAGCGCGCGACCCCAAACTGACT
CGGGTACTGGGCGGAAAAGAGCGTGCCGATTCGGTATTGGCAGCCCTTTCGGCCTTGCCGGACAGTTGCGATGCCTGGGC
GCTGGTGCATGATGCCGCACGGCCTTGTTTGACCCATGGTGATATAGATGCGCTGCTTGCAAGCAGGCTGACGTATCCCC
AGGGCGCGATACTTGCCATGCCTGTGCGGGATACCATGAAGCGGGCAGCGACCGATGGCAGTATCGAAACCACTGTTTGC
CGCGAAGCCCTGTGGCATGCGTTAACGCCGCAGCTTTTTCCGGCAGAGCGCTTGAAACAGCATCTGGAGCAGGCGCTGGC
CGCCGGTGTTTCTATTACCGACGAAGCGTCGGCGATGGAGTGGGCCGGAGTGTATCCGGGGCTGGTCAGTGGCCGCGCCG
ACAATATCAAGGTCACCCACCCGGACGATTTACAGCTGGCGGGGCTTTTTCTTCAGGCCCAGCAGCAACACACTTAA

Upstream 100 bases:

>100_bases
GTAATGAACTTGGTTTGATTGAACAGGGTGAAACTTTCTTCCGGGTTGTGGGTAACGATAGTCGCAGTATCCGCTCTTCG
GAACAGTCACAGGACAATCA

Downstream 100 bases:

>100_bases
GGAATTTTCATGAAAATCAGGATTGGCCACGGTTTTGATGTGCACAAGTTTGGCGCAGACCGTCCACTCATTCTCTGCGG
TATAGAAGTGCCTTACGAAA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNFQSSSSGSIVAIVPAAGIGSRMGATIPKQYLPLLDKPILAHTLQRLLSHPAIDKVIVAVSAEDSWFDSLAEARDPKLT
RVLGGKERADSVLAALSALPDSCDAWALVHDAARPCLTHGDIDALLASRLTYPQGAILAMPVRDTMKRAATDGSIETTVC
REALWHALTPQLFPAERLKQHLEQALAAGVSITDEASAMEWAGVYPGLVSGRADNIKVTHPDDLQLAGLFLQAQQQHT

Sequences:

>Translated_238_residues
MNFQSSSSGSIVAIVPAAGIGSRMGATIPKQYLPLLDKPILAHTLQRLLSHPAIDKVIVAVSAEDSWFDSLAEARDPKLT
RVLGGKERADSVLAALSALPDSCDAWALVHDAARPCLTHGDIDALLASRLTYPQGAILAMPVRDTMKRAATDGSIETTVC
REALWHALTPQLFPAERLKQHLEQALAAGVSITDEASAMEWAGVYPGLVSGRADNIKVTHPDDLQLAGLFLQAQQQHT
>Mature_238_residues
MNFQSSSSGSIVAIVPAAGIGSRMGATIPKQYLPLLDKPILAHTLQRLLSHPAIDKVIVAVSAEDSWFDSLAEARDPKLT
RVLGGKERADSVLAALSALPDSCDAWALVHDAARPCLTHGDIDALLASRLTYPQGAILAMPVRDTMKRAATDGSIETTVC
REALWHALTPQLFPAERLKQHLEQALAAGVSITDEASAMEWAGVYPGLVSGRADNIKVTHPDDLQLAGLFLQAQQQHT

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=227, Percent_Identity=26.8722466960352, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=54.5045045045045, Blast_Score=223, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_SHEAM (A1S4D9)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926915.1
- ProteinModelPortal:   A1S4D9
- SMR:   A1S4D9
- STRING:   A1S4D9
- GeneID:   4603290
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1038
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   A1S4D9
- ProtClustDB:   PRK00155
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25427; Mature: 25427

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQSSSSGSIVAIVPAAGIGSRMGATIPKQYLPLLDKPILAHTLQRLLSHPAIDKVIVA
CCCCCCCCCCEEEEECCCCCCCHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHEEEE
VSAEDSWFDSLAEARDPKLTRVLGGKERADSVLAALSALPDSCDAWALVHDAARPCLTHG
EECCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
DIDALLASRLTYPQGAILAMPVRDTMKRAATDGSIETTVCREALWHALTPQLFPAERLKQ
CHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
HLEQALAAGVSITDEASAMEWAGVYPGLVSGRADNIKVTHPDDLQLAGLFLQAQQQHT
HHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFQSSSSGSIVAIVPAAGIGSRMGATIPKQYLPLLDKPILAHTLQRLLSHPAIDKVIVA
CCCCCCCCCCEEEEECCCCCCCHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHEEEE
VSAEDSWFDSLAEARDPKLTRVLGGKERADSVLAALSALPDSCDAWALVHDAARPCLTHG
EECCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
DIDALLASRLTYPQGAILAMPVRDTMKRAATDGSIETTVCREALWHALTPQLFPAERLKQ
CHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
HLEQALAAGVSITDEASAMEWAGVYPGLVSGRADNIKVTHPDDLQLAGLFLQAQQQHT
HHHHHHHCCCCCCCCHHHHHHHCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA