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The map label for this gene is rumA

Identifier: 119774169

GI number: 119774169

Start: 1248548

End: 1249879

Strand: Direct

Name: rumA

Synonym: Sama_1032

Alternate gene names: 119774169

Gene position: 1248548-1249879 (Clockwise)

Preceding gene: 119774165

Following gene: 119774170

Centisome position: 28.99

GC content: 55.11

Gene sequence:

>1332_bases
ATGGCGCAGTTTTTCAAAGCGAAACCCAATCAAGCGAAAAAGCTCTCCCAAAAGATAGCACTCAAGGTGCAAAGACTCGA
TCACTTGGGGGCCGGCATTGCCGAACATCAGGGCAAGGTGGTGTTTATTCCGGGCGCTTTGCCGGGCGAAACCGTGGAAG
TGCAGCTCACAGAGCAAAAGAAAAACTATGCCCGTGCCAAATTGCAGCGGGTATCAGAGGCAAGCCCCGACCGGCAAACA
CCGCCTTGCCCCTGGTATGGCAAATGCGGTGGCTGCGATTTACAGCATCTGTCGTTGCCACGTCAGCTTGAATACAAGCG
TCAGGCGCTGGGTGATATTGTCAGCCGTTCGGCTGCGCAAACAGTGACAGTCAATACCGACGAAGTAAGCGGTGACAGCT
GGCATTATCGTCGCCGGGCCAGGTTAGCAACTCTCCTCGATAAAGAGACCAACCAGCTTGCACTGGGATTCCGGGAAGAG
GGCAGCAAATCGGTAGTGGGTATCGACAGCTGTGCTGTGCTGGCCAGGCCGTTGTCTGAGCTTATTTCGCCTTTTGCCAC
GCTCCTTAACCGTCTCAAGGGCAAGCAGCGGTTGGGGCATCTAGAGCTGACACAGGCCGCCAATGGGCTTTTTGCCGTGT
TGCGTGTGACTGCCCCGCTGGCTCAAAGCGATAAAAAGCTGCTTTCGGCCTTTGCCGATGAGCGGCAAATTGCCCTCTTG
CTGCAGGGGAATGAAGGGGAGCTTGAGTTTCTCTCCAGCGGCCATGAGTTGCCCTATTACCAATTGGACGGTCTCAAGCT
GGCTTTTGCGCCGGGTAACTTTATTCAGGTCAATGGCGAGGTCAACCAGGCCATGGTGGCACAGGCCATGAACTGGCTCG
ATGTGCATGCCGGTGAGCGGGTGCTCGACCTCTTTTGCGGCGTGGGCAACTTCAGCTTGCCTCTTGCAAAGCAAGGAGCA
GAGGTAGTTGGCGTTGAAGGTGTGCCCGAAATGGTGGCGCAGGCGAAGCAAAACGCTGCCATAAACGGCTTGGATAATCT
CAGTTTTTACTGCGCAGACCTCAGTGAAGATTTGGCCGCCGAGCCCTGGCTTGGCAAAATCGATAAGCTGCTGCTGGACC
CGGCCCGTGCCGGTGCATATGAGAGTCTTAAGTGGCTCAAAAAGATGAAGCCTGCCAAGGTGCTCTATGTGTCCTGTAAT
CCGGCAAGCCTTGCCCGTGATTCAGTGCTGCTTTTTGAGGCAGGATATCAGCTGACCCGCCTTGGTCTGGTGGACATGTT
CCCCCAGACCCATCACAGCGAAGGCATGGCATTGTTCGAATTGGTCAATTAA

Upstream 100 bases:

>100_bases
ATTCATGTGCTGGGTTGTCCCTGATTTCCTTTGAGTTTGAATGTACTAAGACTGCCAGAAACCCGGCTGTCTTAACAGAT
TTTTCTTGTATGAGGCCACA

Downstream 100 bases:

>100_bases
CGGATGAATTTTGCTGGATGTAGCAAAATTAATGGACAATCGGCCCGCTTGGGTTTGACAAGCGCGTGCCAATCCTGAAG
ATAAAGTCTTTAACTTGTTT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAQFFKAKPNQAKKLSQKIALKVQRLDHLGAGIAEHQGKVVFIPGALPGETVEVQLTEQKKNYARAKLQRVSEASPDRQT
PPCPWYGKCGGCDLQHLSLPRQLEYKRQALGDIVSRSAAQTVTVNTDEVSGDSWHYRRRARLATLLDKETNQLALGFREE
GSKSVVGIDSCAVLARPLSELISPFATLLNRLKGKQRLGHLELTQAANGLFAVLRVTAPLAQSDKKLLSAFADERQIALL
LQGNEGELEFLSSGHELPYYQLDGLKLAFAPGNFIQVNGEVNQAMVAQAMNWLDVHAGERVLDLFCGVGNFSLPLAKQGA
EVVGVEGVPEMVAQAKQNAAINGLDNLSFYCADLSEDLAAEPWLGKIDKLLLDPARAGAYESLKWLKKMKPAKVLYVSCN
PASLARDSVLLFEAGYQLTRLGLVDMFPQTHHSEGMALFELVN

Sequences:

>Translated_443_residues
MAQFFKAKPNQAKKLSQKIALKVQRLDHLGAGIAEHQGKVVFIPGALPGETVEVQLTEQKKNYARAKLQRVSEASPDRQT
PPCPWYGKCGGCDLQHLSLPRQLEYKRQALGDIVSRSAAQTVTVNTDEVSGDSWHYRRRARLATLLDKETNQLALGFREE
GSKSVVGIDSCAVLARPLSELISPFATLLNRLKGKQRLGHLELTQAANGLFAVLRVTAPLAQSDKKLLSAFADERQIALL
LQGNEGELEFLSSGHELPYYQLDGLKLAFAPGNFIQVNGEVNQAMVAQAMNWLDVHAGERVLDLFCGVGNFSLPLAKQGA
EVVGVEGVPEMVAQAKQNAAINGLDNLSFYCADLSEDLAAEPWLGKIDKLLLDPARAGAYESLKWLKKMKPAKVLYVSCN
PASLARDSVLLFEAGYQLTRLGLVDMFPQTHHSEGMALFELVN
>Mature_442_residues
AQFFKAKPNQAKKLSQKIALKVQRLDHLGAGIAEHQGKVVFIPGALPGETVEVQLTEQKKNYARAKLQRVSEASPDRQTP
PCPWYGKCGGCDLQHLSLPRQLEYKRQALGDIVSRSAAQTVTVNTDEVSGDSWHYRRRARLATLLDKETNQLALGFREEG
SKSVVGIDSCAVLARPLSELISPFATLLNRLKGKQRLGHLELTQAANGLFAVLRVTAPLAQSDKKLLSAFADERQIALLL
QGNEGELEFLSSGHELPYYQLDGLKLAFAPGNFIQVNGEVNQAMVAQAMNWLDVHAGERVLDLFCGVGNFSLPLAKQGAE
VVGVEGVPEMVAQAKQNAAINGLDNLSFYCADLSEDLAAEPWLGKIDKLLLDPARAGAYESLKWLKKMKPAKVLYVSCNP
ASLARDSVLLFEAGYQLTRLGLVDMFPQTHHSEGMALFELVN

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=209, Percent_Identity=33.9712918660287, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI51173878, Length=209, Percent_Identity=33.9712918660287, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1789148, Length=443, Percent_Identity=46.7268623024831, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1787083, Length=372, Percent_Identity=26.8817204301075, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790403, Length=198, Percent_Identity=31.3131313131313, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322909, Length=423, Percent_Identity=22.9314420803783, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24666579, Length=201, Percent_Identity=30.8457711442786, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_SHEAM (A1S4D3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926909.1
- ProteinModelPortal:   A1S4D3
- SMR:   A1S4D3
- STRING:   A1S4D3
- GeneID:   4603284
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1032
- NMPDR:   fig|326297.7.peg.971
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   IDADNGH
- PhylomeDB:   A1S4D3
- ProtClustDB:   PRK13168
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 48498; Mature: 48367

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 399-399 BINDING 276-276 BINDING 305-305 BINDING 310-310 BINDING 326-326 BINDING 353-353 BINDING 373-373

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAKPNQAKKLSQKIALKVQRLDHLGAGIAEHQGKVVFIPGALPGETVEVQLTEQK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCEEEEEEEHHH
KNYARAKLQRVSEASPDRQTPPCPWYGKCGGCDLQHLSLPRQLEYKRQALGDIVSRSAAQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
TVTVNTDEVSGDSWHYRRRARLATLLDKETNQLALGFREEGSKSVVGIDSCAVLARPLSE
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECHHHHHHHHHHHH
LISPFATLLNRLKGKQRLGHLELTQAANGLFAVLRVTAPLAQSDKKLLSAFADERQIALL
HHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LQGNEGELEFLSSGHELPYYQLDGLKLAFAPGNFIQVNGEVNQAMVAQAMNWLDVHAGER
EECCCCCCHHHCCCCCCCEEEECCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCHH
VLDLFCGVGNFSLPLAKQGAEVVGVEGVPEMVAQAKQNAAINGLDNLSFYCADLSEDLAA
HHHHHHCCCCCCCCHHHCCCCEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCC
EPWLGKIDKLLLDPARAGAYESLKWLKKMKPAKVLYVSCNPASLARDSVLLFEAGYQLTR
CCCHHHHHHHHHCHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCCEEEEECCCCEEH
LGLVDMFPQTHHSEGMALFELVN
HHHHHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
AQFFKAKPNQAKKLSQKIALKVQRLDHLGAGIAEHQGKVVFIPGALPGETVEVQLTEQK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCEEEEEEEHHH
KNYARAKLQRVSEASPDRQTPPCPWYGKCGGCDLQHLSLPRQLEYKRQALGDIVSRSAAQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCC
TVTVNTDEVSGDSWHYRRRARLATLLDKETNQLALGFREEGSKSVVGIDSCAVLARPLSE
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECHHHHHHHHHHHH
LISPFATLLNRLKGKQRLGHLELTQAANGLFAVLRVTAPLAQSDKKLLSAFADERQIALL
HHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LQGNEGELEFLSSGHELPYYQLDGLKLAFAPGNFIQVNGEVNQAMVAQAMNWLDVHAGER
EECCCCCCHHHCCCCCCCEEEECCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCHH
VLDLFCGVGNFSLPLAKQGAEVVGVEGVPEMVAQAKQNAAINGLDNLSFYCADLSEDLAA
HHHHHHCCCCCCCCHHHCCCCEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCC
EPWLGKIDKLLLDPARAGAYESLKWLKKMKPAKVLYVSCNPASLARDSVLLFEAGYQLTR
CCCHHHHHHHHHCHHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCCEEEEECCCCEEH
LGLVDMFPQTHHSEGMALFELVN
HHHHHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA