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The map label for this gene is ribH

Identifier: 119774156

GI number: 119774156

Start: 1234022

End: 1234498

Strand: Direct

Name: ribH

Synonym: Sama_1019

Alternate gene names: 119774156

Gene position: 1234022-1234498 (Clockwise)

Preceding gene: 119774155

Following gene: 119774157

Centisome position: 28.66

GC content: 52.83

Gene sequence:

>477_bases
ATGCACATAGTAGAAGGTCATATCGAAGCCAAATCTGCCAAAGTGGCGATTGTGGTATCACGTTTCAACAGTTTTGTGGT
TGAGAGCCTGCTGTCAGGCGCCATCGATACGCTCAAGCGTTTTGGCCAGGTCAGCGAAGACAACATCACTGTAGTGCGCG
TTCCGGGCGCCTTCGAATTGCCGCTCGCTGCCAAGAAAGTTGCTGCTTCAGGCAAGTTTGACGGCATCATTGCGCTGGGC
GCCGTGATCCGTGGTGGTACTCCTCACTTTGACTTTGTTGCCGGTGAGTGCAACAAGGGACTGGCCCAGGTTTCCCTGGA
ATTCAACACTCCAGTGTCTTTCGGTGTATTGACCACGGATACCATTGAGCAAGCCATCGAGCGCTCAGGTACCAAGGCCG
GCAACAAGGGCGGCGAAGCTGCTCTGGGCCTGCTGGAAATGGTCAATGTACTTAATGCTATTGATAAAGAGCTGTAA

Upstream 100 bases:

>100_bases
TTGGGGCAAAAGCCTGTGGTATGATAGCGCCACTTTTTGTCCCGGTCCGTCCGGGCGGATTGCACCAAGCCGGGTGCCTT
AAGTTAAACCAGGTGAGAGA

Downstream 100 bases:

>100_bases
TTAGTAGGAAAAATCATGAAGCCTTCAGAGCGCCGTAAGGCCCGTCGCCTGGCGGTCCAAGCTATCTATTCCTGGCAACT
GAGCGGCAATAACGTTGCCG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MHIVEGHIEAKSAKVAIVVSRFNSFVVESLLSGAIDTLKRFGQVSEDNITVVRVPGAFELPLAAKKVAASGKFDGIIALG
AVIRGGTPHFDFVAGECNKGLAQVSLEFNTPVSFGVLTTDTIEQAIERSGTKAGNKGGEAALGLLEMVNVLNAIDKEL

Sequences:

>Translated_158_residues
MHIVEGHIEAKSAKVAIVVSRFNSFVVESLLSGAIDTLKRFGQVSEDNITVVRVPGAFELPLAAKKVAASGKFDGIIALG
AVIRGGTPHFDFVAGECNKGLAQVSLEFNTPVSFGVLTTDTIEQAIERSGTKAGNKGGEAALGLLEMVNVLNAIDKEL
>Mature_158_residues
MHIVEGHIEAKSAKVAIVVSRFNSFVVESLLSGAIDTLKRFGQVSEDNITVVRVPGAFELPLAAKKVAASGKFDGIIALG
AVIRGGTPHFDFVAGECNKGLAQVSLEFNTPVSFGVLTTDTIEQAIERSGTKAGNKGGEAALGLLEMVNVLNAIDKEL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=62.987012987013, Blast_Score=202, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6324429, Length=122, Percent_Identity=36.0655737704918, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_SHEAM (A1S4C0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926896.1
- ProteinModelPortal:   A1S4C0
- SMR:   A1S4C0
- STRING:   A1S4C0
- GeneID:   4603271
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1019
- NMPDR:   fig|326297.7.peg.959
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   A1S4C0
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16536; Mature: 16536

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVEGHIEAKSAKVAIVVSRFNSFVVESLLSGAIDTLKRFGQVSEDNITVVRVPGAFEL
CCEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PLAAKKVAASGKFDGIIALGAVIRGGTPHFDFVAGECNKGLAQVSLEFNTPVSFGVLTTD
CHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCEEECEEEHH
TIEQAIERSGTKAGNKGGEAALGLLEMVNVLNAIDKEL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHIVEGHIEAKSAKVAIVVSRFNSFVVESLLSGAIDTLKRFGQVSEDNITVVRVPGAFEL
CCEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
PLAAKKVAASGKFDGIIALGAVIRGGTPHFDFVAGECNKGLAQVSLEFNTPVSFGVLTTD
CHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCEEECEEEHH
TIEQAIERSGTKAGNKGGEAALGLLEMVNVLNAIDKEL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA