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The map label for this gene is glyA

Identifier: 119774151

GI number: 119774151

Start: 1229015

End: 1230268

Strand: Direct

Name: glyA

Synonym: Sama_1014

Alternate gene names: 119774151

Gene position: 1229015-1230268 (Clockwise)

Preceding gene: 119774147

Following gene: 119774152

Centisome position: 28.54

GC content: 55.26

Gene sequence:

>1254_bases
ATGCTGAAGAAGACCATGAATATTGCCGACTATGATCCCGAGCTGTTCAAGGCGATTGAAGACGAAACCCTTCGCCAGGA
AGAGCATATCGAGCTGATTGCCTCCGAGAACTACACTTCTCCCCGCGTGATGCAGGCTCAGGGTTCACAGCTGACCAACA
AGTACGCCGAAGGTTATCCTGGTAAGCGTTACTATGGTGGTTGTGAGTACGTCGACATCGTGGAAACCCTGGCCATCGAG
CGCGCCAAAGAACTCTTCGGTGCCACTTTCGCCAACGTACAGCCACACTCCGGCTCTCAGGCCAACAGCGCCGTGTACAT
GACCCTGCTGCAGCCAGGCGACACTGTATTGGGTATGAACCTGGCCCACGGTGGTCACCTGACCCACGGCTCACCGGTAA
ACTTCTCTGGCAAGCTGTACAACATCATTCCTTACGGTATCGATGAAACCGGCAAGATTGACTACGACGAAATGGAACGT
CTGGCCGTTGAGCACAAGCCAAAGATGATGATTGGCGGTTTCTCCGCCTATTCCGGCATCGTTGACTGGGCCCGCATGCG
TGAAATCGCAGACAAAATTGGTGCTTACCTGTTTGTGGACATGGCTCACGTAGCTGGTCTCGTGGCGGCCGGTGTATATC
CAAACCCGGTTCCACACGCTCACGTAGTCACTTCTACCACTCACAAGACCCTGGCCGGCCCCCGCGGCGGTATCATTCTG
TCTGCTGCCAACGATGAAGACCTGTACAAGAAGCTGAACTCAGCGGTATTCCCAGGTGGTCAGGGTGGCCCATTGATGCA
CGTTATCGCAGGTAAAGCGGTAGCCTTCAAAGAAGCGCTGGAGCCTGAGTTCAAGGTGTACCAGCAGCAGGTTGTGACCA
ACGCCAAAGCCATGGTGGAAGTATTCCTCGAGCGCGGTTACAAAATCGTCTCCGGCGGCACCGACAACCACCTGATGCTG
GTTGACCTGATTGGCCGCGACCTGACCGGTAAAGAAGCCGACGCCGCTCTGGGCGCTGCCAACATCACTGTGAATAAGAA
CTCAGTACCAAACGACCCACGTTCTCCGTTCATCACCTCCGGTATCCGTATCGGTACTCCAGCGATCACCCGTCGTGGTT
TCAAAGAAGCCGAGGCCCGCGAGCTGACTCACTGGATCTGTGATGTACTGGACAACGCCAAAGACGAGAGCGTGATTGCC
CGCGTGAAAGGCCAGGTACTGGAACTGTGCGCTCGTTTCCCTGTTTACGGTTAA

Upstream 100 bases:

>100_bases
CAGAGAGGGATTTTCATCATATTGCGGCTGGGATTGTGATCGATTGCAGAGTAGAATACCGCGCAACCCTACCTATAAGA
TTTGCCAGCTGGAGTGACCA

Downstream 100 bases:

>100_bases
CCTTTTAACCTGAATAGGATACACTCATGGCCGCTGCGATAGCGGCCATTTTTATTTGTATTGCGGTTCAATTTGTCCTT
CATTCAGCACGCCAGGAGGC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLKKTMNIADYDPELFKAIEDETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVETLAIE
RAKELFGATFANVQPHSGSQANSAVYMTLLQPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDETGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWARMREIADKIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
SAANDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVTNAKAMVEVFLERGYKIVSGGTDNHLML
VDLIGRDLTGKEADAALGAANITVNKNSVPNDPRSPFITSGIRIGTPAITRRGFKEAEARELTHWICDVLDNAKDESVIA
RVKGQVLELCARFPVYG

Sequences:

>Translated_417_residues
MLKKTMNIADYDPELFKAIEDETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVETLAIE
RAKELFGATFANVQPHSGSQANSAVYMTLLQPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDETGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWARMREIADKIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
SAANDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVTNAKAMVEVFLERGYKIVSGGTDNHLML
VDLIGRDLTGKEADAALGAANITVNKNSVPNDPRSPFITSGIRIGTPAITRRGFKEAEARELTHWICDVLDNAKDESVIA
RVKGQVLELCARFPVYG
>Mature_417_residues
MLKKTMNIADYDPELFKAIEDETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVETLAIE
RAKELFGATFANVQPHSGSQANSAVYMTLLQPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDETGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWARMREIADKIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
SAANDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVTNAKAMVEVFLERGYKIVSGGTDNHLML
VDLIGRDLTGKEADAALGAANITVNKNSVPNDPRSPFITSGIRIGTPAITRRGFKEAEARELTHWICDVLDNAKDESVIA
RVKGQVLELCARFPVYG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=46.5174129353234, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=46.5174129353234, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=46.5174129353234, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=46.5174129353234, Blast_Score=354, Evalue=8e-98,
Organism=Homo sapiens, GI261862346, Length=401, Percent_Identity=45.8852867830424, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=48.1296758104738, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=45.7583547557841, Blast_Score=308, Evalue=4e-84,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=83.8942307692308, Blast_Score=723, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=409, Percent_Identity=45.9657701711491, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=409, Percent_Identity=45.9657701711491, Blast_Score=343, Evalue=8e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=46.3840399002494, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=412, Percent_Identity=44.6601941747573, Blast_Score=344, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=48, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=48, Blast_Score=367, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_SHEAM (A1S4B5)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926891.1
- ProteinModelPortal:   A1S4B5
- SMR:   A1S4B5
- STRING:   A1S4B5
- GeneID:   4603266
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1014
- NMPDR:   fig|326297.7.peg.954
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   GTSNHLM
- PhylomeDB:   A1S4B5
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45430; Mature: 45430

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKTMNIADYDPELFKAIEDETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHEEEEECCCCCCCCEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDIVETLAIERAKELFGATFANVQPHSGSQANSAVYMTLLQPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDETGKIDYDEMERLAVEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARMREIADKIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAANDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVTNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTDNHLMLVDLIGRDLTGKEADAALGAANITVNKNSVPNDPRSPFITS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCEEC
GIRIGTPAITRRGFKEAEARELTHWICDVLDNAKDESVIARVKGQVLELCARFPVYG
CEEECCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKKTMNIADYDPELFKAIEDETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHEEEEECCCCCCCCEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDIVETLAIERAKELFGATFANVQPHSGSQANSAVYMTLLQPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDETGKIDYDEMERLAVEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARMREIADKIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAANDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVTNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTDNHLMLVDLIGRDLTGKEADAALGAANITVNKNSVPNDPRSPFITS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCEEC
GIRIGTPAITRRGFKEAEARELTHWICDVLDNAKDESVIARVKGQVLELCARFPVYG
CEEECCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA