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The map label for this gene is pnp

Identifier: 119774103

GI number: 119774103

Start: 1167839

End: 1169938

Strand: Direct

Name: pnp

Synonym: Sama_0966

Alternate gene names: 119774103

Gene position: 1167839-1169938 (Clockwise)

Preceding gene: 119774101

Following gene: 119774104

Centisome position: 27.12

GC content: 53.62

Gene sequence:

>2100_bases
GTGAATCCTATCGTAAAAAGTTTTGAGTATGGTCAGCATACAGTCACTCTGGAAACAGGCGTAATCGCTCGTCAAGCCGA
TGGTGCCGTAATGGCAAGCATGGGCGACACAACTGTACTGGTTACCGTTGTGGGCAAGAAGGAAGCAGACCCAGGCCGTG
ACTTCTTCCCACTTACCGTAAACTACCAGGAAAAAACTTACGCAGCTGGTAAGATCCCCGGTGGCTTCTTTAAGCGTGAA
GGCCGTCCATCTGAAGACGAAACTCTGATTGCCCGATTGATCGATCGTCCAATCCGCCCCCTGTTCCCTGATGACTTTAC
CAACGAAGTACAGGTCATCATCACAGTGGTCTCGGTAGACCCTCAGATTGAGCCGGACATTGTTTCTATGATCGGTACTT
CTGCTGCCCTGGCGATTTCAGGTATTCCATTTAACGGTCCTCTGGGTGCTGCCCGCGTGGGTTATGTGAATGGCGAATAC
GTGTTGAACCCAGGTGCCGCTGAACTGGCTGGCAGCGAACTGGATCTGGTGGTTGCCGGTACCCAGAGCGCCGTACTGAT
GGTGGAATCTGAAGCCAAATCTCTGCCAGAAGAAGTCATGCTGGGTGCCGTAGTGTACGGTCATGACCAGCAGCAGGTTG
TTATCAATGCCATCAACGAGTTTGCTGCCGAAGCCGGTAAGCCACGTTGGAACTGGACTGCACCTGTTAAAAATGAAGCC
CTGGTAGCACAAATCAAAGAGCTGGCCGAAGAAGGCCTGACTGCGGCTTACCAGATCATGGCCAAGCAAGAACGTTACGA
AGCCGTTGGTGAAGTAAAGAAAGCCACTATCGCCAAGCTGCAGGAAGCCAATCCTGACGTGAACGTACGTGAAGCTGCCG
ATCTGCTGGGTAGCCTGGAGAAGAACGTCGTTCGCGGCCGCATTATCGCTGGCATGCCACGTATCGACGGCCGTGAGCCA
GACATGATCCGTGCCCTGTCTGTCATGGCAGGTGTACTGCCACGTACCCACGGCTCTGCCCTGTTCACCCGTGGTGAAAC
TCAGGCGTTGGTAACCTGTACTCTGGGTACCGAGCGTGATGCCCAGAAGATTGACTCCATCATGGGCGAGCGCACCAATC
GCTTCATGCTGCACTACAACTTCCCTCCATACTCTGTGGGTGAAACCGGTATGGTTGGCTCGCCAAAGCGCCGCGAAATC
GGCCACGGTAAGCTGGCATGGCGCGGTATCAACGCGGTAATGCCATCTGCCGAAGAGTTCCCATACTCTGTACGTGTGGT
ATCTGAAATCACTGAGTCCAACGGTTCTTCTTCCATGGCCTCTGTGTGCGGTACCTCTCTGGCGCTGATGGATGCCGGTG
TACCTATCAAGACTTCTGTGGCCGGTATTGCCATGGGTCTGGTGAAGGAAGGTGACAACTTCGTGGTTCTGTCTGACATC
CTGGGTGACGAAGACCACCTGGGTGACATGGACTTCAAAGTAGCCGGTACCCGTGATGGTGTTACTGCACTGCAGATGGA
CATCAAAATCGAAGGTATCACCAAAGAAATCATGGAAATCGCTCTGCAGCAGGCTTACGGCGCCCGCGTTCACATCCTGA
ACGTGATGGACCAGGCCATCGGTAGTGCCCGTCCAGATATCTCTGACTTCGCGCCACGTATCACCACCATCAAGATCAAC
CCAGAGAAGATCCGTGATGTTATCGGTAAAGGCGGCGCCGTTATCCGTGCGCTGACTGAAGAAACCGGTACCACCATCGA
GCTGGAAGATGACGGTACTGTGAAGATTGCCTCTAACAATGGCGACGCTACCCGCGAAGCTATCCGCCGCATCGAGGAAA
TCACCTCAGAAGTGGAAGTGGGTCGTCTGTACACAGGTAAGGTTATCCGTATCGTTGACTTCGGTGCCTTCGTGAACATC
CTGCCCGGCAAAGATGGTCTGGTACACATCTCGCAAATCAGCGATGAGCGTGTAGCCAACGTATCTGACCACCTGCAACT
GAACCAGGAAGTGACCGTGAAGGTCATGGAAGTGGATCGTCAGGGTCGTGTGCGTCTGTCTATCAAGGAAGCTCAGGTTA
AAGAACCTGCTGCCGAATAA

Upstream 100 bases:

>100_bases
AGTTAAAGATTTGTCACCAGATTCCACTCTTTAACTATCTACTTGACTTGCAGTATACTTACGCGCGTATTTCAAGGTCA
TTAATTAAGGAATGGGTCAC

Downstream 100 bases:

>100_bases
CAGATAGCCATAACGGTTAATTAAAGGAGACCTCAGGGTCTCCTTTTTTATTGGACAGTGAAAACCGGGCGGACAATTTA
TAATAAATATCAATGGCTGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MNPIVKSFEYGQHTVTLETGVIARQADGAVMASMGDTTVLVTVVGKKEADPGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPDDFTNEVQVIITVVSVDPQIEPDIVSMIGTSAALAISGIPFNGPLGAARVGYVNGEY
VLNPGAAELAGSELDLVVAGTQSAVLMVESEAKSLPEEVMLGAVVYGHDQQQVVINAINEFAAEAGKPRWNWTAPVKNEA
LVAQIKELAEEGLTAAYQIMAKQERYEAVGEVKKATIAKLQEANPDVNVREAADLLGSLEKNVVRGRIIAGMPRIDGREP
DMIRALSVMAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSARPDISDFAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASNNGDATREAIRRIEEITSEVEVGRLYTGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLQLNQEVTVKVMEVDRQGRVRLSIKEAQVKEPAAE

Sequences:

>Translated_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADGAVMASMGDTTVLVTVVGKKEADPGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPDDFTNEVQVIITVVSVDPQIEPDIVSMIGTSAALAISGIPFNGPLGAARVGYVNGEY
VLNPGAAELAGSELDLVVAGTQSAVLMVESEAKSLPEEVMLGAVVYGHDQQQVVINAINEFAAEAGKPRWNWTAPVKNEA
LVAQIKELAEEGLTAAYQIMAKQERYEAVGEVKKATIAKLQEANPDVNVREAADLLGSLEKNVVRGRIIAGMPRIDGREP
DMIRALSVMAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSARPDISDFAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASNNGDATREAIRRIEEITSEVEVGRLYTGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLQLNQEVTVKVMEVDRQGRVRLSIKEAQVKEPAAE
>Mature_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADGAVMASMGDTTVLVTVVGKKEADPGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPDDFTNEVQVIITVVSVDPQIEPDIVSMIGTSAALAISGIPFNGPLGAARVGYVNGEY
VLNPGAAELAGSELDLVVAGTQSAVLMVESEAKSLPEEVMLGAVVYGHDQQQVVINAINEFAAEAGKPRWNWTAPVKNEA
LVAQIKELAEEGLTAAYQIMAKQERYEAVGEVKKATIAKLQEANPDVNVREAADLLGSLEKNVVRGRIIAGMPRIDGREP
DMIRALSVMAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSARPDISDFAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASNNGDATREAIRRIEEITSEVEVGRLYTGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLQLNQEVTVKVMEVDRQGRVRLSIKEAQVKEPAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=711, Percent_Identity=38.5372714486639, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=74.2402315484805, Blast_Score=1054, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=678, Percent_Identity=32.8908554572271, Blast_Score=338, Evalue=6e-93,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=38.1153305203938, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=38.1153305203938, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=38.1153305203938, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=37.5951293759513, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_SHEAM (A1S467)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926843.1
- ProteinModelPortal:   A1S467
- STRING:   A1S467
- GeneID:   4603218
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0966
- NMPDR:   fig|326297.7.peg.907
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   A1S467
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75372; Mature: 75372

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADGAVMASMGDTTVLVTVVGKKEADPGRDFFPLTV
CCCCHHCCCCCCEEEEEECCEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPDDFTNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIVSMIGTSAALAISGIPFNGPLGAARVGYVNGEYVLNPGAAELAGSELDLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEECCCHHHHCCCCEEEEEEC
TQSAVLMVESEAKSLPEEVMLGAVVYGHDQQQVVINAINEFAAEAGKPRWNWTAPVKNEA
CCCEEEEEECHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQIKELAEEGLTAAYQIMAKQERYEAVGEVKKATIAKLQEANPDVNVREAADLLGSLE
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KNVVRGRIIAGMPRIDGREPDMIRALSVMAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSARPDISDFAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASNN
CCCCCCHHHHCCCEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GDATREAIRRIEEITSEVEVGRLYTGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEECCCEEEEECCCCCEEEEECCCCHHHCC
VSDHLQLNQEVTVKVMEVDRQGRVRLSIKEAQVKEPAAE
CHHHHCCCCCEEEEEEEECCCCCEEEEEEHHHCCCCCCC
>Mature Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADGAVMASMGDTTVLVTVVGKKEADPGRDFFPLTV
CCCCHHCCCCCCEEEEEECCEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPDDFTNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIVSMIGTSAALAISGIPFNGPLGAARVGYVNGEYVLNPGAAELAGSELDLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCEEEECCCHHHHCCCCEEEEEEC
TQSAVLMVESEAKSLPEEVMLGAVVYGHDQQQVVINAINEFAAEAGKPRWNWTAPVKNEA
CCCEEEEEECHHHCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQIKELAEEGLTAAYQIMAKQERYEAVGEVKKATIAKLQEANPDVNVREAADLLGSLE
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KNVVRGRIIAGMPRIDGREPDMIRALSVMAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDNFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSARPDISDFAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASNN
CCCCCCHHHHCCCEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GDATREAIRRIEEITSEVEVGRLYTGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEECCCEEEEECCCCCEEEEECCCCHHHCC
VSDHLQLNQEVTVKVMEVDRQGRVRLSIKEAQVKEPAAE
CHHHHCCCCCEEEEEEEECCCCCEEEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA