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The map label for this gene is truB

Identifier: 119774100

GI number: 119774100

Start: 1164226

End: 1165173

Strand: Direct

Name: truB

Synonym: Sama_0963

Alternate gene names: 119774100

Gene position: 1164226-1165173 (Clockwise)

Preceding gene: 119774099

Following gene: 119774101

Centisome position: 27.04

GC content: 56.01

Gene sequence:

>948_bases
ATGGCAAGACGCACCAAAGGGCGCAATATCGACGGTATCGTCCTGCTGGATAAACCCACGGGCATGAGTTCCAATCATGC
CCTGCAGCGGGTTAAGCGCATTTACAATGCGTCCAAAGCAGGCCATACAGGCGCGCTCGATCCTCTGGCGACCGGCATGT
TACCCGTGTGCCTGGGTGAGGCAACCAAGTTTTCTCAGCACCTGCTGGATGCGGATAAACGTTATCTGGTTACTGCCAAG
CTTGGTCAGCGCACTGACACCAGCGATTCCGACGGCGAAGTGGTCAGTGAGCGGCCATTAAACTTCACCCAGGAAGCGCT
GGATGCTGCCTTGGACAGTTTCCGTGGTGATACCCTGCAGGTACCTTCGATGTTTTCGGCGCTTAAGTATCAGGGCCAGC
CCCTGTATAAGTATGCCCGTGAAGGTAAGGAAGTTCCGCGGGAAGCCAGACCCATCACAGTGTTCGAGCTTAACTTCATC
AAGCTTGAAGGTGATGAGCTGACGCTGGATATTCACTGCTCCAAGGGTACATACATACGTACCATCATTGACGATCTCGG
TGAGAAGCTTGGCTGTGGCGCCCATGTGATTATGCTCCGACGCACCGCCGTCGCCGGTTACCCCTACGAGCGTATGGTGA
CCATCGAAGCGCTTGAAGCATTGCTGGAGCAGGCTGAAAAGGAGGGCGTTGAACCCAAGACCTTGCTCGACCCGCTGCTG
CTGCCGATGGATACCGCTGTCGCCAATTTTCCGGAGGTCAATATTCCGGATGCGGTTGCGCCTTATTTGATGAACGGTAA
CCCGGTGCGCGTCGCAGGCGTGTCCAGCGATAGCCTGTTGCGTATTACCCTTGGTGAGGCGCGACGGTTCGTTGGTGTGG
GCGCCATCAATGACGATGGCCTGCTGGCGCCCAAGCGCCTGGTTGTCTTTTACGACGATGAAGCCTAA

Upstream 100 bases:

>100_bases
TGCGCATGTCAAACCTGGTGAGTAAGGTCATTCGTGACGATGAGGCCAAGAAAGGTCACATTGGCGATGAACCCCAGGAA
GGGCAGCGCGAGGAAGACTG

Downstream 100 bases:

>100_bases
AGCGGTTGCTACGGCAGTCGAATGTGGCTAAAATATCGCGTCTTCTTGGCTGAGTTAGTGATCGGCTGAGACAATTTACT
GATATTTTGGAGATATTCAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MARRTKGRNIDGIVLLDKPTGMSSNHALQRVKRIYNASKAGHTGALDPLATGMLPVCLGEATKFSQHLLDADKRYLVTAK
LGQRTDTSDSDGEVVSERPLNFTQEALDAALDSFRGDTLQVPSMFSALKYQGQPLYKYAREGKEVPREARPITVFELNFI
KLEGDELTLDIHCSKGTYIRTIIDDLGEKLGCGAHVIMLRRTAVAGYPYERMVTIEALEALLEQAEKEGVEPKTLLDPLL
LPMDTAVANFPEVNIPDAVAPYLMNGNPVRVAGVSSDSLLRITLGEARRFVGVGAINDDGLLAPKRLVVFYDDEA

Sequences:

>Translated_315_residues
MARRTKGRNIDGIVLLDKPTGMSSNHALQRVKRIYNASKAGHTGALDPLATGMLPVCLGEATKFSQHLLDADKRYLVTAK
LGQRTDTSDSDGEVVSERPLNFTQEALDAALDSFRGDTLQVPSMFSALKYQGQPLYKYAREGKEVPREARPITVFELNFI
KLEGDELTLDIHCSKGTYIRTIIDDLGEKLGCGAHVIMLRRTAVAGYPYERMVTIEALEALLEQAEKEGVEPKTLLDPLL
LPMDTAVANFPEVNIPDAVAPYLMNGNPVRVAGVSSDSLLRITLGEARRFVGVGAINDDGLLAPKRLVVFYDDEA
>Mature_314_residues
ARRTKGRNIDGIVLLDKPTGMSSNHALQRVKRIYNASKAGHTGALDPLATGMLPVCLGEATKFSQHLLDADKRYLVTAKL
GQRTDTSDSDGEVVSERPLNFTQEALDAALDSFRGDTLQVPSMFSALKYQGQPLYKYAREGKEVPREARPITVFELNFIK
LEGDELTLDIHCSKGTYIRTIIDDLGEKLGCGAHVIMLRRTAVAGYPYERMVTIEALEALLEQAEKEGVEPKTLLDPLLL
PMDTAVANFPEVNIPDAVAPYLMNGNPVRVAGVSSDSLLRITLGEARRFVGVGAINDDGLLAPKRLVVFYDDEA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=227, Percent_Identity=35.6828193832599, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4503337, Length=173, Percent_Identity=28.3236994219653, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI215599015, Length=173, Percent_Identity=28.3236994219653, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI2367200, Length=312, Percent_Identity=67.3076923076923, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17553978, Length=335, Percent_Identity=26.2686567164179, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=267, Percent_Identity=26.9662921348315, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=161, Percent_Identity=37.2670807453416, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364187, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364185, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364183, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI62471759, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17975520, Length=270, Percent_Identity=28.1481481481481, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_SHEAM (A1S464)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926840.1
- ProteinModelPortal:   A1S464
- SMR:   A1S464
- STRING:   A1S464
- GeneID:   4603215
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0963
- NMPDR:   fig|326297.7.peg.904
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   A1S464
- ProtClustDB:   PRK05033
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34502; Mature: 34371

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRTKGRNIDGIVLLDKPTGMSSNHALQRVKRIYNASKAGHTGALDPLATGMLPVCLGE
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCH
ATKFSQHLLDADKRYLVTAKLGQRTDTSDSDGEVVSERPLNFTQEALDAALDSFRGDTLQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCEEE
VPSMFSALKYQGQPLYKYAREGKEVPREARPITVFELNFIKLEGDELTLDIHCSKGTYIR
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCEEEEEEEECCCCCHH
TIIDDLGEKLGCGAHVIMLRRTAVAGYPYERMVTIEALEALLEQAEKEGVEPKTLLDPLL
HHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LPMDTAVANFPEVNIPDAVAPYLMNGNPVRVAGVSSDSLLRITLGEARRFVGVGAINDDG
CCHHHHHHCCCCCCCCHHHHHHEECCCEEEEEECCCCCEEEEEECCCCEEEEEEEECCCC
LLAPKRLVVFYDDEA
CCCCCEEEEEECCCC
>Mature Secondary Structure 
ARRTKGRNIDGIVLLDKPTGMSSNHALQRVKRIYNASKAGHTGALDPLATGMLPVCLGE
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCH
ATKFSQHLLDADKRYLVTAKLGQRTDTSDSDGEVVSERPLNFTQEALDAALDSFRGDTLQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCEEE
VPSMFSALKYQGQPLYKYAREGKEVPREARPITVFELNFIKLEGDELTLDIHCSKGTYIR
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCEEEEEEEECCCCCHH
TIIDDLGEKLGCGAHVIMLRRTAVAGYPYERMVTIEALEALLEQAEKEGVEPKTLLDPLL
HHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LPMDTAVANFPEVNIPDAVAPYLMNGNPVRVAGVSSDSLLRITLGEARRFVGVGAINDDG
CCHHHHHHCCCCCCCCHHHHHHEECCCEEEEEECCCCCEEEEEECCCCEEEEEEEECCCC
LLAPKRLVVFYDDEA
CCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA