Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 119774097

GI number: 119774097

Start: 1159587

End: 1161086

Strand: Direct

Name: nusA [H]

Synonym: Sama_0960

Alternate gene names: 119774097

Gene position: 1159587-1161086 (Clockwise)

Preceding gene: 119774096

Following gene: 119774098

Centisome position: 26.93

GC content: 53.4

Gene sequence:

>1500_bases
ATGAATAAAGAGATTCTGCTGGTCGCTGAGGCGGTTTCAAACGAGAAAGCCGTTCCGCGTGAAAAGATTTTCGAGGCGTT
GGAGACAGCTCTGGCCACGGCAACCAAGAAAAAGTACGAAGGTGATATCGACGTTCGTGTAGCCATCGATCGCAAGACCG
GTGACTATGAAACCTTCCGTCGCTGGATGGTGGTAGAGGATAACGGCGAAGCGCTGGAAAATCCTTACCGTGAGATCACC
CTCGAAGCCGCCCAATATGAAAATCCTGAAATCCAGGTGGGCGAGTTTATTGAGGACGAAATCGAGTCTGTGGTTTTCGA
CCGTATCACTACCCAAACTGCCAAGCAGGTTATCGTGCAAAAAGTGCGCGAAGCCGAACGTGCTCAGGTTGTTGAACAGT
TTTACGACAAAGAAGGCGAAATCATCACTGGCGTGGTGAAGAAGAGCAATCGTGACAGTGTCATTGTGGATTTGGGCAAC
AACGCCGACGGCGTGCTGTACAAAGAAGACCTGATTGCCCGCGAATCTTTCCGTCCAGGCGATCGCGTTCGCGCACTGCT
CTATGCTGTGCGTCCTGAGGCCCGCGGTGCTCAGTTGTACCTGACCCGTACCAAGCCTGAAATGCTGATTGAGCTCTTCC
GTGTGGAAGTGCCTGAAATCGCAGATGAGATGATCCAGGTGATGGGTGCTGCCCGTGATCCAGGCAGCCGCGCCAAGATT
GCTGTGAAGTCTAACGACCGTCGCATCGATCCTATCGGCGCCTGTGTGGGTATGCGTGGTGCCCGTGTTCAGGCCGTGTC
CAACGAACTCGGTGGTGAGCGCGTGGATATCGTGCTGTGGGACGATAACCCAGCGCAATTCGTGATCAACGCCATGGCGC
CAGCCGATGTGGCTTCTATCATCGTGGATGAAGATAACCACTCTATGGACATCGCCGTGGAAGCTGATTCTCTGGCCCAG
GCTATTGGCCGTAATGGTCAGAACGTGCGTTTGGCCACTCAACTGACCGGTTGGGAGTTGAACGTGATGACAGTGGCAGA
CATGAATGCCAAGCACCAGGCAGAAAGTGCCAAGGTGGTTGCGCTGTTTATGGCGTCACTGGATGTAGATGAAGATTTTG
CTCAGGTGCTGGCCGATGAAGGCTTCACCTCACTGGAAGAAGTGGCTTATGTACCGGTTGCCGAACTCTTGGCAATCGAT
GGCTTCGACGAGGACATCGTTGAAGCGCTGCGTGAGCGCGCCAAAGCGGCCATTTCTACCCGTGCGTTGGCGTCTGAAGA
AGCGCTGGACGGTGCAGAGCCGAGTGAAGAATTGCTGAATCTTGAAGGCATGGATCGCCACCTCGCGTTTGTACTTGCAA
GCCGTGGTGTAGTGACGCTGGAAGATTTGGCCGAACAAGGCATTGATGATTTGATCGAAATAGAAGAATTGACAGAAGAG
AAAGCCGGTGCGCTGATCATGGCTGCCCGTAACATCTGTTGGTTTGGCGAAGAGTCTTAA

Upstream 100 bases:

>100_bases
CAAAGTCGATGGCAAGACATTGGTCGTTGCCCTGGACAATATCCGCAAAGGCAACATTATTGCGAAGTTTTGATGGTTTC
AAGGTGAACGAGGCAAGACA

Downstream 100 bases:

>100_bases
GTCGATCAACAGGGGAGGTAACCTATGACGGAAATTACGGTAGAGAAACTAGCCACAGAAGTCGGTAAAACTGTTGACAG
ACTTATTGAACAGTTTGCCC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVAIDRKTGDYETFRRWMVVEDNGEALENPYREIT
LEAAQYENPEIQVGEFIEDEIESVVFDRITTQTAKQVIVQKVREAERAQVVEQFYDKEGEIITGVVKKSNRDSVIVDLGN
NADGVLYKEDLIARESFRPGDRVRALLYAVRPEARGAQLYLTRTKPEMLIELFRVEVPEIADEMIQVMGAARDPGSRAKI
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQFVINAMAPADVASIIVDEDNHSMDIAVEADSLAQ
AIGRNGQNVRLATQLTGWELNVMTVADMNAKHQAESAKVVALFMASLDVDEDFAQVLADEGFTSLEEVAYVPVAELLAID
GFDEDIVEALRERAKAAISTRALASEEALDGAEPSEELLNLEGMDRHLAFVLASRGVVTLEDLAEQGIDDLIEIEELTEE
KAGALIMAARNICWFGEES

Sequences:

>Translated_499_residues
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVAIDRKTGDYETFRRWMVVEDNGEALENPYREIT
LEAAQYENPEIQVGEFIEDEIESVVFDRITTQTAKQVIVQKVREAERAQVVEQFYDKEGEIITGVVKKSNRDSVIVDLGN
NADGVLYKEDLIARESFRPGDRVRALLYAVRPEARGAQLYLTRTKPEMLIELFRVEVPEIADEMIQVMGAARDPGSRAKI
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQFVINAMAPADVASIIVDEDNHSMDIAVEADSLAQ
AIGRNGQNVRLATQLTGWELNVMTVADMNAKHQAESAKVVALFMASLDVDEDFAQVLADEGFTSLEEVAYVPVAELLAID
GFDEDIVEALRERAKAAISTRALASEEALDGAEPSEELLNLEGMDRHLAFVLASRGVVTLEDLAEQGIDDLIEIEELTEE
KAGALIMAARNICWFGEES
>Mature_499_residues
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVAIDRKTGDYETFRRWMVVEDNGEALENPYREIT
LEAAQYENPEIQVGEFIEDEIESVVFDRITTQTAKQVIVQKVREAERAQVVEQFYDKEGEIITGVVKKSNRDSVIVDLGN
NADGVLYKEDLIARESFRPGDRVRALLYAVRPEARGAQLYLTRTKPEMLIELFRVEVPEIADEMIQVMGAARDPGSRAKI
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQFVINAMAPADVASIIVDEDNHSMDIAVEADSLAQ
AIGRNGQNVRLATQLTGWELNVMTVADMNAKHQAESAKVVALFMASLDVDEDFAQVLADEGFTSLEEVAYVPVAELLAID
GFDEDIVEALRERAKAAISTRALASEEALDGAEPSEELLNLEGMDRHLAFVLASRGVVTLEDLAEQGIDDLIEIEELTEE
KAGALIMAARNICWFGEES

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=499, Percent_Identity=73.74749498998, Blast_Score=758, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55145; Mature: 55145

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVAIDRKTGDYETFR
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
RWMVVEDNGEALENPYREITLEAAQYENPEIQVGEFIEDEIESVVFDRITTQTAKQVIVQ
EEEEEECCCCHHHCCHHHEEEEHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVREAERAQVVEQFYDKEGEIITGVVKKSNRDSVIVDLGNNADGVLYKEDLIARESFRPG
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCH
DRVRALLYAVRPEARGAQLYLTRTKPEMLIELFRVEVPEIADEMIQVMGAARDPGSRAKI
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEE
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQFVINAMAPADVASI
EEECCCCCCCHHHHHHCCCCCEEEHHHHHCCCCEEEEEEECCCCHHEEEECCCCCCEEEE
IVDEDNHSMDIAVEADSLAQAIGRNGQNVRLATQLTGWELNVMTVADMNAKHQAESAKVV
EEECCCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEEEECCCCHHHHHHHHHH
ALFMASLDVDEDFAQVLADEGFTSLEEVAYVPVAELLAIDGFDEDIVEALRERAKAAIST
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RALASEEALDGAEPSEELLNLEGMDRHLAFVLASRGVVTLEDLAEQGIDDLIEIEELTEE
HHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHH
KAGALIMAARNICWFGEES
HCCEEEEEECCEEECCCCC
>Mature Secondary Structure
MNKEILLVAEAVSNEKAVPREKIFEALETALATATKKKYEGDIDVRVAIDRKTGDYETFR
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
RWMVVEDNGEALENPYREITLEAAQYENPEIQVGEFIEDEIESVVFDRITTQTAKQVIVQ
EEEEEECCCCHHHCCHHHEEEEHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVREAERAQVVEQFYDKEGEIITGVVKKSNRDSVIVDLGNNADGVLYKEDLIARESFRPG
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCEEEHHHHHHHCCCCCH
DRVRALLYAVRPEARGAQLYLTRTKPEMLIELFRVEVPEIADEMIQVMGAARDPGSRAKI
HHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEE
AVKSNDRRIDPIGACVGMRGARVQAVSNELGGERVDIVLWDDNPAQFVINAMAPADVASI
EEECCCCCCCHHHHHHCCCCCEEEHHHHHCCCCEEEEEEECCCCHHEEEECCCCCCEEEE
IVDEDNHSMDIAVEADSLAQAIGRNGQNVRLATQLTGWELNVMTVADMNAKHQAESAKVV
EEECCCCCEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEEEECCCCHHHHHHHHHH
ALFMASLDVDEDFAQVLADEGFTSLEEVAYVPVAELLAIDGFDEDIVEALRERAKAAIST
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RALASEEALDGAEPSEELLNLEGMDRHLAFVLASRGVVTLEDLAEQGIDDLIEIEELTEE
HHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHCCCCEEHHHHHHCCHHHHHHHHHHHHH
KAGALIMAARNICWFGEES
HCCEEEEEECCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]