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The map label for this gene is alsT [H]

Identifier: 119774053

GI number: 119774053

Start: 1108397

End: 1109839

Strand: Direct

Name: alsT [H]

Synonym: Sama_0916

Alternate gene names: 119774053

Gene position: 1108397-1109839 (Clockwise)

Preceding gene: 119774047

Following gene: 119774054

Centisome position: 25.74

GC content: 50.8

Gene sequence:

>1443_bases
ATGTTAGAAACTATTGTTAATTTTCTTAATGCCCTGCTATGGGGCAAGCTGCTGGTTTACGGACTGGTGGGTGCGGGGCT
TTACTTTACCGTTCGCCTGGCCTTTATCCAACTGACTCACTTTAAACATGGTATCAAGGTTATGACCATCAGCCGTCAGG
GTGGTGATGGTGGCTTATCTTCTTTCCAGGTGTTCTGTACCAGCATGGCAGCACGTGTTGGTGCTGGTAATATGGCCGGT
GTTGCTGTAGCCATTGGCACTGCAGGACCAGGAGCTGTGTTCTGGATGTGGGCGATTGCAGTACTCGGTATGGCAACTGC
CATGATTGAATCGACCCTCGCACAGGTCTACAAGGTGAAAGATGAAGATGGCCAGTTCCGCGGTGGTCCTGCCTATTATA
TGGAGAAGGGCTTGGGTCAGCGCTGGATGGGAGCACTGTTCTCTGTCTTCCTGATTTTGGCATTTGGTTTGGTATTCAAC
GCAGTTCAGGCCAATACCATCACCGGTGCGCTGGAAAGGGTATTCGGATTCGAACCCATCTATGTTGGCATTGGAATAGT
GGTAGCCAGTGGGTTCGTCATTATGGGCGGCCTTCGTAAAGTGGCGCGTGTATCAGAAATCGTTGTGCCCTTTATGGCTC
TGGTCTACCTGTTGATTGCCTCTGTGGTTGTCGTGGTGAACCTAGATAAACTGCCAGAAGTATTCAGCCTGATAATTAAG
AGTGCATTTGGTTGGCAAGAAGCCGTAGCCGGCGGTGTTGCATACAGTGTGGCTCAGGCCATGCAAGCAGGTATTGCCCG
TGGCCTCTTCTCCAACGAAGCAGGGATGGGCAGTGCCGCGAACGTAGCTGCCAGCGCCACACCCAACCCAAATCATCCAG
CTTCTCAGGGATTTGTGCAGATGATGGGAGTGTTTGTCGATACGCTGGTGATTTGTACCGCCACTGCGGCCATTATTTTG
CTGGCCGGTGACATGGGTGCAGAAAGTGATGGTATTAAACTCACCATCGACGCCTTGACCAGCCATGTAGGAGATTGGGG
CGGCGCATTCATCGCCTTTGCGATTTTCCTGTTCTGCTTTACATCTATTATCGCTAACTATTCCTACGCTGAAACCAATG
TCATGTTCCTGTCAGGTAACCGCAAAACTGCGTTGCCGCTGTTCAGGTTACTGGTGCTGGGCATGGTGATGTTTGGTGCT
ATGGCAAAAATCAGTCTGGTTTGGGATCTCGCGGATGTTTCCATGGGCCTGATGGCAATAGTGAATATTATCGCGCTGGT
ACTACTTTCAGGTCTCGCTATTCGGGTGATCGATGACTATCGGGAGCAGCTTAAAAAAGGTGACATGCCGGTATTTGACC
GAAGTAAGTTTCCAGAGCTGGATGGACAGGTCGAAACTGACATCTGGCCCGAGAATGCCAAAGCTAGGGCCAACGAAGCC
TGA

Upstream 100 bases:

>100_bases
TACAGCTATTCATGCGCTTAAGGATGATCTTAAGCAATGTTGCGGCAGCGGCGAGAGAAAGGGATGCATCGCCATTGCAG
GAAGGAAGACGAACAAGACT

Downstream 100 bases:

>100_bases
GCCGATTAGTGCAATCCACAAAACCACGCCAATGCGGCGTGGTTTTTTTATCTCTCTTGGGTATCATAAGGCTTAACTGA
ATTGACGAGAGAAACCCATG

Product: sodium:alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLETIVNFLNALLWGKLLVYGLVGAGLYFTVRLAFIQLTHFKHGIKVMTISRQGGDGGLSSFQVFCTSMAARVGAGNMAG
VAVAIGTAGPGAVFWMWAIAVLGMATAMIESTLAQVYKVKDEDGQFRGGPAYYMEKGLGQRWMGALFSVFLILAFGLVFN
AVQANTITGALERVFGFEPIYVGIGIVVASGFVIMGGLRKVARVSEIVVPFMALVYLLIASVVVVVNLDKLPEVFSLIIK
SAFGWQEAVAGGVAYSVAQAMQAGIARGLFSNEAGMGSAANVAASATPNPNHPASQGFVQMMGVFVDTLVICTATAAIIL
LAGDMGAESDGIKLTIDALTSHVGDWGGAFIAFAIFLFCFTSIIANYSYAETNVMFLSGNRKTALPLFRLLVLGMVMFGA
MAKISLVWDLADVSMGLMAIVNIIALVLLSGLAIRVIDDYREQLKKGDMPVFDRSKFPELDGQVETDIWPENAKARANEA

Sequences:

>Translated_480_residues
MLETIVNFLNALLWGKLLVYGLVGAGLYFTVRLAFIQLTHFKHGIKVMTISRQGGDGGLSSFQVFCTSMAARVGAGNMAG
VAVAIGTAGPGAVFWMWAIAVLGMATAMIESTLAQVYKVKDEDGQFRGGPAYYMEKGLGQRWMGALFSVFLILAFGLVFN
AVQANTITGALERVFGFEPIYVGIGIVVASGFVIMGGLRKVARVSEIVVPFMALVYLLIASVVVVVNLDKLPEVFSLIIK
SAFGWQEAVAGGVAYSVAQAMQAGIARGLFSNEAGMGSAANVAASATPNPNHPASQGFVQMMGVFVDTLVICTATAAIIL
LAGDMGAESDGIKLTIDALTSHVGDWGGAFIAFAIFLFCFTSIIANYSYAETNVMFLSGNRKTALPLFRLLVLGMVMFGA
MAKISLVWDLADVSMGLMAIVNIIALVLLSGLAIRVIDDYREQLKKGDMPVFDRSKFPELDGQVETDIWPENAKARANEA
>Mature_480_residues
MLETIVNFLNALLWGKLLVYGLVGAGLYFTVRLAFIQLTHFKHGIKVMTISRQGGDGGLSSFQVFCTSMAARVGAGNMAG
VAVAIGTAGPGAVFWMWAIAVLGMATAMIESTLAQVYKVKDEDGQFRGGPAYYMEKGLGQRWMGALFSVFLILAFGLVFN
AVQANTITGALERVFGFEPIYVGIGIVVASGFVIMGGLRKVARVSEIVVPFMALVYLLIASVVVVVNLDKLPEVFSLIIK
SAFGWQEAVAGGVAYSVAQAMQAGIARGLFSNEAGMGSAANVAASATPNPNHPASQGFVQMMGVFVDTLVICTATAAIIL
LAGDMGAESDGIKLTIDALTSHVGDWGGAFIAFAIFLFCFTSIIANYSYAETNVMFLSGNRKTALPLFRLLVLGMVMFGA
MAKISLVWDLADVSMGLMAIVNIIALVLLSGLAIRVIDDYREQLKKGDMPVFDRSKFPELDGQVETDIWPENAKARANEA

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=470, Percent_Identity=47.0212765957447, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51050; Mature: 51050

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETIVNFLNALLWGKLLVYGLVGAGLYFTVRLAFIQLTHFKHGIKVMTISRQGGDGGLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHH
SFQVFCTSMAARVGAGNMAGVAVAIGTAGPGAVFWMWAIAVLGMATAMIESTLAQVYKVK
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEDGQFRGGPAYYMEKGLGQRWMGALFSVFLILAFGLVFNAVQANTITGALERVFGFEPI
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YVGIGIVVASGFVIMGGLRKVARVSEIVVPFMALVYLLIASVVVVVNLDKLPEVFSLIIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAFGWQEAVAGGVAYSVAQAMQAGIARGLFSNEAGMGSAANVAASATPNPNHPASQGFVQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHHHHH
MMGVFVDTLVICTATAAIILLAGDMGAESDGIKLTIDALTSHVGDWGGAFIAFAIFLFCF
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHH
TSIIANYSYAETNVMFLSGNRKTALPLFRLLVLGMVMFGAMAKISLVWDLADVSMGLMAI
HHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNIIALVLLSGLAIRVIDDYREQLKKGDMPVFDRSKFPELDGQVETDIWPENAKARANEA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLETIVNFLNALLWGKLLVYGLVGAGLYFTVRLAFIQLTHFKHGIKVMTISRQGGDGGLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHH
SFQVFCTSMAARVGAGNMAGVAVAIGTAGPGAVFWMWAIAVLGMATAMIESTLAQVYKVK
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEDGQFRGGPAYYMEKGLGQRWMGALFSVFLILAFGLVFNAVQANTITGALERVFGFEPI
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YVGIGIVVASGFVIMGGLRKVARVSEIVVPFMALVYLLIASVVVVVNLDKLPEVFSLIIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAFGWQEAVAGGVAYSVAQAMQAGIARGLFSNEAGMGSAANVAASATPNPNHPASQGFVQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHHHHH
MMGVFVDTLVICTATAAIILLAGDMGAESDGIKLTIDALTSHVGDWGGAFIAFAIFLFCF
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHH
TSIIANYSYAETNVMFLSGNRKTALPLFRLLVLGMVMFGAMAKISLVWDLADVSMGLMAI
HHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNIIALVLLSGLAIRVIDDYREQLKKGDMPVFDRSKFPELDGQVETDIWPENAKARANEA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]