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The map label for this gene is trmD

Identifier: 119774028

GI number: 119774028

Start: 1078882

End: 1079634

Strand: Direct

Name: trmD

Synonym: Sama_0891

Alternate gene names: 119774028

Gene position: 1078882-1079634 (Clockwise)

Preceding gene: 119774027

Following gene: 119774029

Centisome position: 25.05

GC content: 54.18

Gene sequence:

>753_bases
ATGTGGTTAGGGGTAATCACCCTGTTTCCCGAAATGTTTCGTGCCATCACTGATTTTGGGGTCACGGGTCGGGCCATCAA
AAATGGCCTGCTGGAGTTGCACACGTGGAATCCCCGTGATTTCACCCATGACAGACACAGAACCGTGGACGACAGGCCTT
ATGGCGGCGGACCGGGAATGTTGATGATGGTGCAACCACTGAAAGATGCCATCCAGGCTGCCAGAACGGCGGCGGGTGAC
GGAGCCAAGGTGATATATCTGTCTCCACAGGGGCGCAAGCTGACACAGCGGGGCGCAACTGAGCTGGCAGAAACACAAAA
GTTGATTTTGGTGTGCGGACGTTACGAAGGTATCGATGAGCGCATCATTCAGACTGAAGTGGACGAGGAATGGTCAATCG
GCGACTACGTGCTTTCGGGCGGTGAGTTACCGGCAATGACCCTGATTGATGCAGTGTCCCGTCTGGTACCCGGCGTGCTG
GGTAAACAGGCTTCGGCGGAGCAGGACTCTTTCTCCGACGGTTTGCTCGATTGTCCTCATTACACCCGGCCCGAATCTTT
GGATGGTCTTGAGGTGCCGGCAGTGCTGCTCGGTGGCAACCACGAAGACATTAGACGCTGGCGTCTTAAACAAAGTCTCG
GAAGAACTTTTTTAAGAAGGCCGGAATTATTTGAAAATCTAGCTCTGACTGACGAACAAACCCGCCTGCTGGCGCAGTTT
GTCGACGAAATGGACAGTCCACAGAAGTCGTAG

Upstream 100 bases:

>100_bases
GATTCCGTATCTGCCTGACCAGTTTGTCCTTGAGGTCAAACTGTCAGAAAAACAGATTATTGTGGATTGGGATCCTGACT
TCTAAATCGAGGTACCAAAC

Downstream 100 bases:

>100_bases
CTCAGTTAATCCTAGTATGGAGTTTACATGAACAACATCATCAAAATGCTCAACGATGAGCAAATGAAAAAAGACGTTCC
TGATTTTGGCCCAGGTGATA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MWLGVITLFPEMFRAITDFGVTGRAIKNGLLELHTWNPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLKDAIQAARTAAGD
GAKVIYLSPQGRKLTQRGATELAETQKLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDAVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLEVPAVLLGGNHEDIRRWRLKQSLGRTFLRRPELFENLALTDEQTRLLAQF
VDEMDSPQKS

Sequences:

>Translated_250_residues
MWLGVITLFPEMFRAITDFGVTGRAIKNGLLELHTWNPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLKDAIQAARTAAGD
GAKVIYLSPQGRKLTQRGATELAETQKLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDAVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLEVPAVLLGGNHEDIRRWRLKQSLGRTFLRRPELFENLALTDEQTRLLAQF
VDEMDSPQKS
>Mature_250_residues
MWLGVITLFPEMFRAITDFGVTGRAIKNGLLELHTWNPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLKDAIQAARTAAGD
GAKVIYLSPQGRKLTQRGATELAETQKLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDAVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGLEVPAVLLGGNHEDIRRWRLKQSLGRTFLRRPELFENLALTDEQTRLLAQF
VDEMDSPQKS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=248, Percent_Identity=79.8387096774193, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_SHEAM (A1S3Z2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926768.1
- ProteinModelPortal:   A1S3Z2
- SMR:   A1S3Z2
- STRING:   A1S3Z2
- GeneID:   4603143
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0891
- NMPDR:   fig|326297.7.peg.840
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- PhylomeDB:   A1S3Z2
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27890; Mature: 27890

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLGVITLFPEMFRAITDFGVTGRAIKNGLLELHTWNPRDFTHDRHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCC
LMMVQPLKDAIQAARTAAGDGAKVIYLSPQGRKLTQRGATELAETQKLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHH
RIIQTEVDEEWSIGDYVLSGGELPAMTLIDAVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGLEVPAVLLGGNHEDIRRWRLKQSLGRTFLRRPELFENLALTDEQTRLLAQF
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
VDEMDSPQKS
HHHHCCCCCC
>Mature Secondary Structure
MWLGVITLFPEMFRAITDFGVTGRAIKNGLLELHTWNPRDFTHDRHRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCC
LMMVQPLKDAIQAARTAAGDGAKVIYLSPQGRKLTQRGATELAETQKLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHH
RIIQTEVDEEWSIGDYVLSGGELPAMTLIDAVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGLEVPAVLLGGNHEDIRRWRLKQSLGRTFLRRPELFENLALTDEQTRLLAQF
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
VDEMDSPQKS
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA