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The map label for this gene is tyrS [H]

Identifier: 119773987

GI number: 119773987

Start: 1037269

End: 1038468

Strand: Direct

Name: tyrS [H]

Synonym: Sama_0850

Alternate gene names: 119773987

Gene position: 1037269-1038468 (Clockwise)

Preceding gene: 119773984

Following gene: 119773995

Centisome position: 24.09

GC content: 54.25

Gene sequence:

>1200_bases
ATGGCTGATTTGGACCAGGTATTAGCAGAAATTAAACGGGGCACAGACGAGATCCTGCTCGAAGCAGATCTGATTGAGAA
ACTGAAAGAGGGCCGTCCACTGCGGGTGAAGCTGGGTGCCGATCCTACCGCTCCCGATATCCATCTGGGCCACACGGTTA
TTCTGAACAAACTGCGTCTGTTTCAGGAGCTGGGGCACGAAGTTATCTTCCTTATCGGTGACTTCACCGGCATGGTAGGT
GACCCAAGTGGTAAAAACAGTACCCGTCCGCCACTGACCCGTGAGCAGGTTCTGGCCAACGCTGAAACCTACAAGGAACA
GGTGTACAAAATTCTCGATCCGGCCAAGACGCGCATCGAATTCAACTCCAGCTGGCTTGAGCAGCTTGGTGCTGCCGGCA
TGATCCGCCTGGCTTCCCATCAAACCGTGGCCCGCATGCTGGAACGTGATGATTTTAAAAAGCGTTATGGCAGCGGTCAG
CCCATCGCCATCCATGAGTTTATGTACCCGCTGCTGCAGGGTTGGGACTCGGTTGCGCTGAAGGCAGACATTGAACTGGG
TGGTACCGATCAGAAGTTCAACCTGCTGATGGGCCGGGAGCTGCAAAAGGCCGAAGGTCAGAAGCCGCAGACAGTGATTA
TGATGCCGCTGCTGGAAGGTCTCGATGGCGTCAAGAAGATGTCCAAGTCTGCCCACAACTATATAGGTGTGAGCGAAGCG
CCGGATGAAATGTTCGGTAAGCTGATGTCTATTTCAGACGAGCTGATGTGGCGCTACTTCGATCTGTTGTCGTTCCGCCC
ATTGGCAGAAGTACAGCAGTTCAAGGCCGATGTGGAAGCCGGAGCCAACCCAAGAGACATCAAGATCGCGCTCGCCAAGG
AAATCATTGCCCGTTTCCACGACCAGGCCGCCGCCGATGCTGCTCATCAGAACTTTATCGACCGTTTCCAGAAGGGGGCT
ATCCCCGATGATATCGAAGAAGTAACCCTGAGCGCAGGTGCTGAAGGTATGGCAGTGGCCAACCTGCTCAAAGAAGCTGG
TCTCGTGGGTTCGACCTCCGATGCCATGCGAATGATTAAGCAGGGAGCGGTGAAAATGGACGGTGAAAAGCTGGAAGATG
CCAAGCAGGTATTCAGTGCCGGTCTGAGCGCCGTATTCCAGGTTGGCAAGCGCAAGTTTGCCAAGGTGACTCTGAGCTAA

Upstream 100 bases:

>100_bases
TGTCGATTGGGCCTAGTGTACACACTTTCATTTGCCCAGGCTAACAAGTAACATAGGTCTCTTTGATTGAAATCGGATAA
CCACAGAGGTAGCTGCCAAC

Downstream 100 bases:

>100_bases
TACTGCTCTTTCACCTCATAAAACAAAACGGGCGCCTGAGGCGCCCGTTTTGTTTTTTAAGATTCTGTTGGCAGACAATC
TTTTGAACAGATGGCGTTTA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MADLDQVLAEIKRGTDEILLEADLIEKLKEGRPLRVKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIEFNSSWLEQLGAAGMIRLASHQTVARMLERDDFKKRYGSGQ
PIAIHEFMYPLLQGWDSVALKADIELGGTDQKFNLLMGRELQKAEGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEA
PDEMFGKLMSISDELMWRYFDLLSFRPLAEVQQFKADVEAGANPRDIKIALAKEIIARFHDQAAADAAHQNFIDRFQKGA
IPDDIEEVTLSAGAEGMAVANLLKEAGLVGSTSDAMRMIKQGAVKMDGEKLEDAKQVFSAGLSAVFQVGKRKFAKVTLS

Sequences:

>Translated_399_residues
MADLDQVLAEIKRGTDEILLEADLIEKLKEGRPLRVKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIEFNSSWLEQLGAAGMIRLASHQTVARMLERDDFKKRYGSGQ
PIAIHEFMYPLLQGWDSVALKADIELGGTDQKFNLLMGRELQKAEGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEA
PDEMFGKLMSISDELMWRYFDLLSFRPLAEVQQFKADVEAGANPRDIKIALAKEIIARFHDQAAADAAHQNFIDRFQKGA
IPDDIEEVTLSAGAEGMAVANLLKEAGLVGSTSDAMRMIKQGAVKMDGEKLEDAKQVFSAGLSAVFQVGKRKFAKVTLS
>Mature_398_residues
ADLDQVLAEIKRGTDEILLEADLIEKLKEGRPLRVKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVGD
PSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIEFNSSWLEQLGAAGMIRLASHQTVARMLERDDFKKRYGSGQP
IAIHEFMYPLLQGWDSVALKADIELGGTDQKFNLLMGRELQKAEGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEAP
DEMFGKLMSISDELMWRYFDLLSFRPLAEVQQFKADVEAGANPRDIKIALAKEIIARFHDQAAADAAHQNFIDRFQKGAI
PDDIEEVTLSAGAEGMAVANLLKEAGLVGSTSDAMRMIKQGAVKMDGEKLEDAKQVFSAGLSAVFQVGKRKFAKVTLS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=408, Percent_Identity=24.7549019607843, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1787925, Length=408, Percent_Identity=26.2254901960784, Blast_Score=122, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=377, Percent_Identity=26.2599469496021, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325160, Length=304, Percent_Identity=25, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=24.3781094527363, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44094; Mature: 43963

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLDQVLAEIKRGTDEILLEADLIEKLKEGRPLRVKLGADPTAPDIHLGHTVILNKLRL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEE
FNSSWLEQLGAAGMIRLASHQTVARMLERDDFKKRYGSGQPIAIHEFMYPLLQGWDSVAL
ECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEE
KADIELGGTDQKFNLLMGRELQKAEGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEA
EEEEEECCCCCHHHHHHCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDEMFGKLMSISDELMWRYFDLLSFRPLAEVQQFKADVEAGANPRDIKIALAKEIIARFH
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DQAAADAAHQNFIDRFQKGAIPDDIEEVTLSAGAEGMAVANLLKEAGLVGSTSDAMRMIK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
QGAVKMDGEKLEDAKQVFSAGLSAVFQVGKRKFAKVTLS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure 
ADLDQVLAEIKRGTDEILLEADLIEKLKEGRPLRVKLGADPTAPDIHLGHTVILNKLRL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKEQVYKILDPAKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEE
FNSSWLEQLGAAGMIRLASHQTVARMLERDDFKKRYGSGQPIAIHEFMYPLLQGWDSVAL
ECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCEEE
KADIELGGTDQKFNLLMGRELQKAEGQKPQTVIMMPLLEGLDGVKKMSKSAHNYIGVSEA
EEEEEECCCCCHHHHHHCCHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDEMFGKLMSISDELMWRYFDLLSFRPLAEVQQFKADVEAGANPRDIKIALAKEIIARFH
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DQAAADAAHQNFIDRFQKGAIPDDIEEVTLSAGAEGMAVANLLKEAGLVGSTSDAMRMIK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
QGAVKMDGEKLEDAKQVFSAGLSAVFQVGKRKFAKVTLS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]