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The map label for this gene is amtB [C]

Identifier: 119773789

GI number: 119773789

Start: 806488

End: 807738

Strand: Direct

Name: amtB [C]

Synonym: Sama_0651

Alternate gene names: 119773789

Gene position: 806488-807738 (Clockwise)

Preceding gene: 119773788

Following gene: 119773790

Centisome position: 18.73

GC content: 54.84

Gene sequence:

>1251_bases
ATGGAAGAAATCAGCAAACTGGGCCTGACAGTGGCCGAACTCAGATTCGCACTGGATACCTTTTACTTTTTGATCTCCGG
GGCCTTGGTCATGTGGATGGCGGCAGGCTTCGCCATGTTGGAAGCGGGTTTGGTGCGCTCCAAAAACACCACTGAGATCC
TCACCAAAAACGTGTGTCTCTATTCAATTGCCTGTATCACCTACCTGATAGTGGGCTACAACATCATGTATGTAGACAAC
CCTGAAGCGGGTTGGTTGCCAAGCTTCGGTACCCTGATTGGTACCCAGGCCGCCGATGCCGACCACTCCCTGGAATCAGA
CTTTTTCTTCCAGGTGGTATTTGTGGCAACCTCTATGTCGATTGTGTCCGGAGCCGTGGCTGAACGCATGAAACTCTGGG
CCTTCCTGGCGTTTTCTGTGGTGATGACTGCAGTTATCTATCCGGTCGAAGGTTTCTGGACCTGGGGTGGCGGTTTTGTC
AGTGCAGCAGGTTTCGTGGACTTTGCCGGTAGCGGTATTGTGCACATGGCCGGCGCTGCCGCCGCACTGTCCGGCGTGAT
TTTGCTGGGCGCCCGCAAAGGTAAATATGGCCCATCGGGTCAGGTCAACCCTATCCCCGGCTCCAACCTGCCAATGGCGA
CTCTGGGTATGTTTATCCTGTGGATGGGCTGGTTTGGCTTTAACGGTGGCTCCCAGTTGATGGTGTCTGATGCCGCCAAC
GCCGCCTCTGTCGGTAAGATCTTCGTCAATACCAACTCGGCTGCGGCTTTCGGTGCGATTTCCGCCCTTGTCGTTTGCAA
AATGATTTGGGGCAAGGCCGATTTAACCATGATCCTGAACGGTGCGCTGGCAGGTCTGGTGGCCATTACCGCAGACCCCC
TGTCACCTTCCCTTGAGTTCGCCGCAGCCATTGGTTTGCTGGCAGGTGCTCTGGTCGTGTTCTCTATCGTGGGCTTTGAT
CGCATCAAGATTGATGACCCCGTAGGTGCTATATCTGTACACGGTGTGGCCGGGGTGCTGGGTTTGCTGTTGGTACCTGC
ATCCAATGCCGATGCGACCCTGGGCGGACAGCTGTTTGGTATTTGTGCCATCTTCGCCTGGACTTTCGGCGCCTCTTTCC
TTGTGTGGTTTGTCCTTAAGGTGACCATGGGGATCCGTGTCAGTGAAGAAGAAGAGTACAATGGTATGGATGCCTCCGAC
TGTGGTATCGATGCCTACCCCGAGTTCGTGACGGTGAAAACCGCCGGTTAA

Upstream 100 bases:

>100_bases
GCAAAATCGGTGACGGTAAAATCTTTGTCACCGATTTAGAGCAAGCGGTCCGAATTCGGACCGGTGAAACTGACGACGAA
GCACTGTAAGGGGAAGCGAG

Downstream 100 bases:

>100_bases
AGGCAGTCAAACTCCGAAAAACAGCAGGCTCAGGCCTGCTGTTTTTGTTTTGCTTGTATTTGCCAAAGCTGGGGAGTGAT
ACTGATTTAAGCCTTGATAT

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MEEISKLGLTVAELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCLYSIACITYLIVGYNIMYVDN
PEAGWLPSFGTLIGTQAADADHSLESDFFFQVVFVATSMSIVSGAVAERMKLWAFLAFSVVMTAVIYPVEGFWTWGGGFV
SAAGFVDFAGSGIVHMAGAAAALSGVILLGARKGKYGPSGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
AASVGKIFVNTNSAAAFGAISALVVCKMIWGKADLTMILNGALAGLVAITADPLSPSLEFAAAIGLLAGALVVFSIVGFD
RIKIDDPVGAISVHGVAGVLGLLLVPASNADATLGGQLFGICAIFAWTFGASFLVWFVLKVTMGIRVSEEEEYNGMDASD
CGIDAYPEFVTVKTAG

Sequences:

>Translated_416_residues
MEEISKLGLTVAELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCLYSIACITYLIVGYNIMYVDN
PEAGWLPSFGTLIGTQAADADHSLESDFFFQVVFVATSMSIVSGAVAERMKLWAFLAFSVVMTAVIYPVEGFWTWGGGFV
SAAGFVDFAGSGIVHMAGAAAALSGVILLGARKGKYGPSGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
AASVGKIFVNTNSAAAFGAISALVVCKMIWGKADLTMILNGALAGLVAITADPLSPSLEFAAAIGLLAGALVVFSIVGFD
RIKIDDPVGAISVHGVAGVLGLLLVPASNADATLGGQLFGICAIFAWTFGASFLVWFVLKVTMGIRVSEEEEYNGMDASD
CGIDAYPEFVTVKTAG
>Mature_416_residues
MEEISKLGLTVAELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCLYSIACITYLIVGYNIMYVDN
PEAGWLPSFGTLIGTQAADADHSLESDFFFQVVFVATSMSIVSGAVAERMKLWAFLAFSVVMTAVIYPVEGFWTWGGGFV
SAAGFVDFAGSGIVHMAGAAAALSGVILLGARKGKYGPSGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
AASVGKIFVNTNSAAAFGAISALVVCKMIWGKADLTMILNGALAGLVAITADPLSPSLEFAAAIGLLAGALVVFSIVGFD
RIKIDDPVGAISVHGVAGVLGLLLVPASNADATLGGQLFGICAIFAWTFGASFLVWFVLKVTMGIRVSEEEEYNGMDASD
CGIDAYPEFVTVKTAG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=412, Percent_Identity=30.3398058252427, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=34.8314606741573, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17550182, Length=430, Percent_Identity=33.953488372093, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32564042, Length=426, Percent_Identity=27.9342723004695, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI133931100, Length=334, Percent_Identity=28.7425149700599, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=27.8688524590164, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=28.0285035629454, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=409, Percent_Identity=27.1393643031785, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078314, Length=407, Percent_Identity=32.9238329238329, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43499; Mature: 43499

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEISKLGLTVAELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YSIACITYLIVGYNIMYVDNPEAGWLPSFGTLIGTQAADADHSLESDFFFQVVFVATSMS
HHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLAFSVVMTAVIYPVEGFWTWGGGFVSAAGFVDFAGSGIVHMAGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHH
AALSGVILLGARKGKYGPSGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
AASVGKIFVNTNSAAAFGAISALVVCKMIWGKADLTMILNGALAGLVAITADPLSPSLEF
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCHHHH
AAAIGLLAGALVVFSIVGFDRIKIDDPVGAISVHGVAGVLGLLLVPASNADATLGGQLFG
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHEECCCCCCCCCCHHHHH
ICAIFAWTFGASFLVWFVLKVTMGIRVSEEEEYNGMDASDCGIDAYPEFVTVKTAG
HHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MEEISKLGLTVAELRFALDTFYFLISGALVMWMAAGFAMLEAGLVRSKNTTEILTKNVCL
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YSIACITYLIVGYNIMYVDNPEAGWLPSFGTLIGTQAADADHSLESDFFFQVVFVATSMS
HHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
IVSGAVAERMKLWAFLAFSVVMTAVIYPVEGFWTWGGGFVSAAGFVDFAGSGIVHMAGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHH
AALSGVILLGARKGKYGPSGQVNPIPGSNLPMATLGMFILWMGWFGFNGGSQLMVSDAAN
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
AASVGKIFVNTNSAAAFGAISALVVCKMIWGKADLTMILNGALAGLVAITADPLSPSLEF
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCHHHH
AAAIGLLAGALVVFSIVGFDRIKIDDPVGAISVHGVAGVLGLLLVPASNADATLGGQLFG
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHEECCCCCCCCCCHHHHH
ICAIFAWTFGASFLVWFVLKVTMGIRVSEEEEYNGMDASDCGIDAYPEFVTVKTAG
HHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]