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The map label for this gene is cca [H]

Identifier: 119718898

GI number: 119718898

Start: 4965705

End: 4967156

Strand: Reverse

Name: cca [H]

Synonym: Noca_4681

Alternate gene names: 119718898

Gene position: 4967156-4965705 (Counterclockwise)

Preceding gene: 119718906

Following gene: 119718897

Centisome position: 99.62

GC content: 73.35

Gene sequence:

>1452_bases
GTGCCCGACGCCGTGCCCCCGCCGCTCTCCATGGCCGATGCCCAGCGGGCGGTCACCGCGGAGCTGGACCGGATCGCGCC
CGTCATCGACGACCTCGGCGAGCGGTTCACGCAGGCCGGGCACGAGCTGGCGCTGGTCGGCGGGCCGGTGCGCGATGCCA
TGCTCGGCCGCCAGCACAACGACCTCGACTTCACGACGTCCGCGCGTCCCGAGCAGACCGAGGCGCTGCTGAAGGGCTGG
GCGGACGCGGTCTGGGACATGGGCCGCGCCTTCGGCACCATCGGCTGCCGCAAGGGCGAGTGGCAGGTGGAGATCACGAC
CTACCGCTCCGAGGCCTACGACCCGGACTCGCGCAAGCCCACCGTCGACTTCGGGGACACCCTCGCCGGAGACCTCGGGC
GCCGGGACTTCACGGTGAACGCGATGGCGGTCCGGGTGCCCGGGCGCGAGCTCGAGGACCCGTACGGCGGGGTGGTCGAC
CTCGCCGAGCGGGTGCTGCGCACCCCGGGACGGCCCGAGGACTCCTTCTCCGACGACCCGCTGCGGATGATGCGCGCCGC
GCGGTTCGCCGCCCAGCTCGGGTTCGGCGTCGACCCGGCTGTCGTCGCGGCGATGACCGCGATGGCCGGGCGGATCGAGA
TCATCTCCGCCGAGCGGGTCCGCGACGAGCTGGTCAAGCTGGTGTGCGCGCCGTACCCCCGCCTCGGGCTGCGGCTCCTG
GTCGACACCGGGCTGGCCGCGCTGGTGCTCCCCGAGCTGCCCGCGCTCGCGCTGGAGCGCGACGAGCACCACCGCCACAA
GGACGTCTACGAGCACACGCTGACCGTGCTCGAGCAGGCGATCGACCTGGAGTCGCGGCTGCCGGGCGGCGGCCCCGACT
TCGTCACCCGCTTCGCCGCGCTCATGCACGACGTCGGCAAGCCGCGGACCCGCCGCTTCATCGACGACGGGACCGTGACG
TTCCACCACCACGACGTGGTCGGCGCCAAGATGACCCGCAAGCGGATGCGGGCGCTGCGGTTCTCCAACGACGAGACCGA
CGCCGTCACCAAGCTGGTCGAGCTGCACCTGCGCTTCCACGGCTACGGCACCGGCGAGTGGACCGACTCCGCCGTGCGCC
GCTACGTCCGCGACGCCGGCGACCAGCTCGGACGCCTGCACGTCGTCACCCGTGCCGACTGCACCACCCGCAACCAGCGC
AAGGCCGACCGGCTCCGCCGGACCTACGACGACCTCGAGGCGCGGATCGCCCGGCTCTCCGAGCAGGAGGAGCTCGACGC
CCTCCGCCCCGACCTGGACGGCAACCAGATCATGGAGATCCTCGGCATCGGCCCGGGCCGTGAGGTCGGGGCCGCGTACC
AGTACCTCCTCGAGCTGCGCATGGACCGCGGGCCGATGGCCGAGGAGGAGGCCCGGGCCGCACTGCTGGAGTGGGCCGCC
TCCCGCTCCTGA

Upstream 100 bases:

>100_bases
GGGCAGGCAGCAGCGACAGCGGGCGAAGCACGGCAGCGACTCTATCCGGGGATGCTGTGCCGGTCCGGGCCCCGCTCCGC
AACCACTAGACTCGACTCCC

Downstream 100 bases:

>100_bases
CGACCGCTGGAACCAGACCCGCCGCAAGAACCGTCGAGTCGGGAGTCCTGGCGGTCGGTGGCGCTCGGTGGGCGACGACC
GCGCCGTAGGGTGCCGTCAT

Product: HDIG domain-containing protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MPDAVPPPLSMADAQRAVTAELDRIAPVIDDLGERFTQAGHELALVGGPVRDAMLGRQHNDLDFTTSARPEQTEALLKGW
ADAVWDMGRAFGTIGCRKGEWQVEITTYRSEAYDPDSRKPTVDFGDTLAGDLGRRDFTVNAMAVRVPGRELEDPYGGVVD
LAERVLRTPGRPEDSFSDDPLRMMRAARFAAQLGFGVDPAVVAAMTAMAGRIEIISAERVRDELVKLVCAPYPRLGLRLL
VDTGLAALVLPELPALALERDEHHRHKDVYEHTLTVLEQAIDLESRLPGGGPDFVTRFAALMHDVGKPRTRRFIDDGTVT
FHHHDVVGAKMTRKRMRALRFSNDETDAVTKLVELHLRFHGYGTGEWTDSAVRRYVRDAGDQLGRLHVVTRADCTTRNQR
KADRLRRTYDDLEARIARLSEQEELDALRPDLDGNQIMEILGIGPGREVGAAYQYLLELRMDRGPMAEEEARAALLEWAA
SRS

Sequences:

>Translated_483_residues
MPDAVPPPLSMADAQRAVTAELDRIAPVIDDLGERFTQAGHELALVGGPVRDAMLGRQHNDLDFTTSARPEQTEALLKGW
ADAVWDMGRAFGTIGCRKGEWQVEITTYRSEAYDPDSRKPTVDFGDTLAGDLGRRDFTVNAMAVRVPGRELEDPYGGVVD
LAERVLRTPGRPEDSFSDDPLRMMRAARFAAQLGFGVDPAVVAAMTAMAGRIEIISAERVRDELVKLVCAPYPRLGLRLL
VDTGLAALVLPELPALALERDEHHRHKDVYEHTLTVLEQAIDLESRLPGGGPDFVTRFAALMHDVGKPRTRRFIDDGTVT
FHHHDVVGAKMTRKRMRALRFSNDETDAVTKLVELHLRFHGYGTGEWTDSAVRRYVRDAGDQLGRLHVVTRADCTTRNQR
KADRLRRTYDDLEARIARLSEQEELDALRPDLDGNQIMEILGIGPGREVGAAYQYLLELRMDRGPMAEEEARAALLEWAA
SRS
>Mature_482_residues
PDAVPPPLSMADAQRAVTAELDRIAPVIDDLGERFTQAGHELALVGGPVRDAMLGRQHNDLDFTTSARPEQTEALLKGWA
DAVWDMGRAFGTIGCRKGEWQVEITTYRSEAYDPDSRKPTVDFGDTLAGDLGRRDFTVNAMAVRVPGRELEDPYGGVVDL
AERVLRTPGRPEDSFSDDPLRMMRAARFAAQLGFGVDPAVVAAMTAMAGRIEIISAERVRDELVKLVCAPYPRLGLRLLV
DTGLAALVLPELPALALERDEHHRHKDVYEHTLTVLEQAIDLESRLPGGGPDFVTRFAALMHDVGKPRTRRFIDDGTVTF
HHHDVVGAKMTRKRMRALRFSNDETDAVTKLVELHLRFHGYGTGEWTDSAVRRYVRDAGDQLGRLHVVTRADCTTRNQRK
ADRLRRTYDDLEARIARLSEQEELDALRPDLDGNQIMEILGIGPGREVGAAYQYLLELRMDRGPMAEEEARAALLEWAAS
RS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=233, Percent_Identity=31.3304721030043, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI87081691, Length=299, Percent_Identity=29.7658862876254, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789436, Length=337, Percent_Identity=31.4540059347181, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71995920, Length=212, Percent_Identity=29.7169811320755, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=488, Percent_Identity=24.1803278688525, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281360112, Length=230, Percent_Identity=31.7391304347826, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357337, Length=230, Percent_Identity=31.7391304347826, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53818; Mature: 53686

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAVPPPLSMADAQRAVTAELDRIAPVIDDLGERFTQAGHELALVGGPVRDAMLGRQHN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCC
DLDFTTSARPEQTEALLKGWADAVWDMGRAFGTIGCRKGEWQVEITTYRSEAYDPDSRKP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCC
TVDFGDTLAGDLGRRDFTVNAMAVRVPGRELEDPYGGVVDLAERVLRTPGRPEDSFSDDP
CCCCCCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCH
LRMMRAARFAAQLGFGVDPAVVAAMTAMAGRIEIISAERVRDELVKLVCAPYPRLGLRLL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHH
VDTGLAALVLPELPALALERDEHHRHKDVYEHTLTVLEQAIDLESRLPGGGPDFVTRFAA
HHCCHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LMHDVGKPRTRRFIDDGTVTFHHHDVVGAKMTRKRMRALRFSNDETDAVTKLVELHLRFH
HHHHHCCCHHHHEECCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GYGTGEWTDSAVRRYVRDAGDQLGRLHVVTRADCTTRNQRKADRLRRTYDDLEARIARLS
CCCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
EQEELDALRPDLDGNQIMEILGIGPGREVGAAYQYLLELRMDRGPMAEEEARAALLEWAA
CHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SRS
CCC
>Mature Secondary Structure 
PDAVPPPLSMADAQRAVTAELDRIAPVIDDLGERFTQAGHELALVGGPVRDAMLGRQHN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCC
DLDFTTSARPEQTEALLKGWADAVWDMGRAFGTIGCRKGEWQVEITTYRSEAYDPDSRKP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCC
TVDFGDTLAGDLGRRDFTVNAMAVRVPGRELEDPYGGVVDLAERVLRTPGRPEDSFSDDP
CCCCCCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCH
LRMMRAARFAAQLGFGVDPAVVAAMTAMAGRIEIISAERVRDELVKLVCAPYPRLGLRLL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHH
VDTGLAALVLPELPALALERDEHHRHKDVYEHTLTVLEQAIDLESRLPGGGPDFVTRFAA
HHCCHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LMHDVGKPRTRRFIDDGTVTFHHHDVVGAKMTRKRMRALRFSNDETDAVTKLVELHLRFH
HHHHHCCCHHHHEECCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GYGTGEWTDSAVRRYVRDAGDQLGRLHVVTRADCTTRNQRKADRLRRTYDDLEARIARLS
CCCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
EQEELDALRPDLDGNQIMEILGIGPGREVGAAYQYLLELRMDRGPMAEEEARAALLEWAA
CHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SRS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA