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The map label for this gene is yafA [C]

Identifier: 119718695

GI number: 119718695

Start: 4731861

End: 4732991

Strand: Reverse

Name: yafA [C]

Synonym: Noca_4476

Alternate gene names: 119718695

Gene position: 4732991-4731861 (Counterclockwise)

Preceding gene: 119718699

Following gene: 119718694

Centisome position: 94.93

GC content: 75.42

Gene sequence:

>1131_bases
TTGCAGTACGGTCTGCAACGGTGCGCGCGGAGGGGGTGTCAGGTGGACGACCTCGTCGACTCCGCGGTGTCCCACTGGGG
CCCGCGGTTCACGGTCAACGGCGTGACGGCCGCCGACTTCGACCGGGTGACCGCCGGGATCGGGAGCTGGGACGACTGGT
GCGCCGCCTGGGTGGCCGCCGGCGCGGCGCACGAGGCGCTCGGGCGAGATGCCCTCGCCGAGGGCCGGACCCGCTCGGCC
GGCGAGCACCTCGCCCAGGCCGCCGTCTACCACCACTTCGCGAAGTTCGTCTTCGTCTGCGACCTCGACCAGATGCGCGC
GGCCCACGCCCGCGCGGTCGCCTGCCTCGACGCCGCCCTGCCGCACCTCGACCCGCCGGGCCGTCGCCTCGAGATCCCGT
TCGAGGGCAGCCGGCTGGCCGGCGTGCTCCGGCTGCCCACCGGGCCGGGCCCGCACCCGGTCGTGGTGCTGCTCTCCGGC
CTGGACTCGGCCAAGGAGGAGCTCCGCTCGACCGAGCAGACCTTCTTGGACCGCGGGCTGGCGACGCTGAGCGTCGACGG
CCCCGGCCAGGGCGAGGCGGAGTACGACCTGCCGATCCGCGGCGACTGGGCGCCGGTGGCCGAGGCCCTCTGGGCGGCGC
TCGGCACCCTGCCGGAGGTGGACCGCGACCGGCTCGGCCTCTGGGGCGTCAGCCTCGGCGGCTACTACGCACCCCGCGTG
GCGGCCGCGCTCGGCGAGCGGGCCCGTGCCTGCGTGGCGCTGGCCGGGCCGTTCAACTTCGGCGGGTGCTGGGACCGGCT
GCCGCAGCTGACCCGGGACACGTTCCGGATCCGCTCCGGCGCCGCCAGCGAGGAGGAGGCCCGGAGGATCGCGCTGACCC
TCGACCTCGAGGAGACCGCCCGCGACGTGGTCGCCCCGCTGCTGATCGTCTTCGGACGCCAGGACCGGCTGATCCCCTGG
GAGCACGCCGAGCGCCTGCGCGACGCCGTCTCGGGACCGGTCGAGCTGCTGATGCTCGAGGACGGCAACCACGGCTGCGC
CAACGTCGCGCCCTGGCACCGGCCCCGCACCGCCGACTGGCTGGCCACCCGGCTCGCCGTACCCGCACTACTGACGAAGG
CAGGATCATGA

Upstream 100 bases:

>100_bases
CGTCCGGCACTCTAGCGGTTTGTGCAGTCGATTGCAGCAACGTCCAACGCAGGCCAGCGCTACTTGACATCACACCGCGG
AGCCGTAAGGCTGCACTCGA

Downstream 100 bases:

>100_bases
TGGACACCACCGTTCCCCAGCCCGGCTCGCTGACGGTCGGCTGGATCGGCGCCGGCAGGATGGGTGCCGCGATGGCGAGC
CGTCTGGCGCGGGCCGGCGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MQYGLQRCARRGCQVDDLVDSAVSHWGPRFTVNGVTAADFDRVTAGIGSWDDWCAAWVAAGAAHEALGRDALAEGRTRSA
GEHLAQAAVYHHFAKFVFVCDLDQMRAAHARAVACLDAALPHLDPPGRRLEIPFEGSRLAGVLRLPTGPGPHPVVVLLSG
LDSAKEELRSTEQTFLDRGLATLSVDGPGQGEAEYDLPIRGDWAPVAEALWAALGTLPEVDRDRLGLWGVSLGGYYAPRV
AAALGERARACVALAGPFNFGGCWDRLPQLTRDTFRIRSGAASEEEARRIALTLDLEETARDVVAPLLIVFGRQDRLIPW
EHAERLRDAVSGPVELLMLEDGNHGCANVAPWHRPRTADWLATRLAVPALLTKAGS

Sequences:

>Translated_376_residues
MQYGLQRCARRGCQVDDLVDSAVSHWGPRFTVNGVTAADFDRVTAGIGSWDDWCAAWVAAGAAHEALGRDALAEGRTRSA
GEHLAQAAVYHHFAKFVFVCDLDQMRAAHARAVACLDAALPHLDPPGRRLEIPFEGSRLAGVLRLPTGPGPHPVVVLLSG
LDSAKEELRSTEQTFLDRGLATLSVDGPGQGEAEYDLPIRGDWAPVAEALWAALGTLPEVDRDRLGLWGVSLGGYYAPRV
AAALGERARACVALAGPFNFGGCWDRLPQLTRDTFRIRSGAASEEEARRIALTLDLEETARDVVAPLLIVFGRQDRLIPW
EHAERLRDAVSGPVELLMLEDGNHGCANVAPWHRPRTADWLATRLAVPALLTKAGS
>Mature_376_residues
MQYGLQRCARRGCQVDDLVDSAVSHWGPRFTVNGVTAADFDRVTAGIGSWDDWCAAWVAAGAAHEALGRDALAEGRTRSA
GEHLAQAAVYHHFAKFVFVCDLDQMRAAHARAVACLDAALPHLDPPGRRLEIPFEGSRLAGVLRLPTGPGPHPVVVLLSG
LDSAKEELRSTEQTFLDRGLATLSVDGPGQGEAEYDLPIRGDWAPVAEALWAALGTLPEVDRDRLGLWGVSLGGYYAPRV
AAALGERARACVALAGPFNFGGCWDRLPQLTRDTFRIRSGAASEEEARRIALTLDLEETARDVVAPLLIVFGRQDRLIPW
EHAERLRDAVSGPVELLMLEDGNHGCANVAPWHRPRTADWLATRLAVPALLTKAGS

Specific function: L-nicotine is used as a growth substrate. Plays a role in nicotine catabolism by cleaving a C-C bond in 2,6- dihydroxypseudooxyicotine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0255 family [H]

Homologues:

Organism=Escherichia coli, GI1786434, Length=323, Percent_Identity=25.077399380805, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010520 [H]

Pfam domain/function: PF06500 DUF1100 [H]

EC number: NA

Molecular weight: Translated: 40603; Mature: 40603

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYGLQRCARRGCQVDDLVDSAVSHWGPRFTVNGVTAADFDRVTAGIGSWDDWCAAWVAA
CCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
GAAHEALGRDALAEGRTRSAGEHLAQAAVYHHFAKFVFVCDLDQMRAAHARAVACLDAAL
HHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHC
PHLDPPGRRLEIPFEGSRLAGVLRLPTGPGPHPVVVLLSGLDSAKEELRSTEQTFLDRGL
CCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
ATLSVDGPGQGEAEYDLPIRGDWAPVAEALWAALGTLPEVDRDRLGLWGVSLGGYYAPRV
EEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHH
AAALGERARACVALAGPFNFGGCWDRLPQLTRDTFRIRSGAASEEEARRIALTLDLEETA
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEEEHHHHH
RDVVAPLLIVFGRQDRLIPWEHAERLRDAVSGPVELLMLEDGNHGCANVAPWHRPRTADW
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
LATRLAVPALLTKAGS
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQYGLQRCARRGCQVDDLVDSAVSHWGPRFTVNGVTAADFDRVTAGIGSWDDWCAAWVAA
CCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
GAAHEALGRDALAEGRTRSAGEHLAQAAVYHHFAKFVFVCDLDQMRAAHARAVACLDAAL
HHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHC
PHLDPPGRRLEIPFEGSRLAGVLRLPTGPGPHPVVVLLSGLDSAKEELRSTEQTFLDRGL
CCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
ATLSVDGPGQGEAEYDLPIRGDWAPVAEALWAALGTLPEVDRDRLGLWGVSLGGYYAPRV
EEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHH
AAALGERARACVALAGPFNFGGCWDRLPQLTRDTFRIRSGAASEEEARRIALTLDLEETA
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEEEHHHHH
RDVVAPLLIVFGRQDRLIPWEHAERLRDAVSGPVELLMLEDGNHGCANVAPWHRPRTADW
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
LATRLAVPALLTKAGS
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11514508; 12618462 [H]