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The map label for this gene is yfjR [H]

Identifier: 119718694

GI number: 119718694

Start: 4730905

End: 4731861

Strand: Reverse

Name: yfjR [H]

Synonym: Noca_4475

Alternate gene names: 119718694

Gene position: 4731861-4730905 (Counterclockwise)

Preceding gene: 119718695

Following gene: 119718693

Centisome position: 94.91

GC content: 72.0

Gene sequence:

>957_bases
ATGGACACCACCGTTCCCCAGCCCGGCTCGCTGACGGTCGGCTGGATCGGCGCCGGCAGGATGGGTGCCGCGATGGCGAG
CCGTCTGGCGCGGGCCGGCGAGGACGTCACCGTCTGGAACCGGACCCGGGCCAAGGCCGAGGCGCTGAGCGAGGTCGGCT
GCGGGGTCGCGGACACGATCGCCGACCTGCGCGGCCACGACGTCGTCTTCACGATGGTCTCGACGCCCGCCGACCTCGAG
CAGGTGCTCGTCGGCGAGGGCGGCCTGCTCGCGGACCCGGCCCGGGTCCCGGCCGTCGTCGTGGACTGCTCCACGGTCTC
CACGGAGTCCTCCGCCGCGATGCGGGCCGCCTGCAGCGAGCGCGGGGTGGCCTTCCTCGCCGCACCGGTCAGCGGCAACG
GCAAGGTCGTCCGGGCCGGCGGGCTCAGCCTCGTGGTCTCCGGGCCCGAGGAGACCTACGAGCGGGTCGCCCACCTGCTC
GACCACCTCGGCAAGGGCGCGACGTACGTCGGGGAGGGCGAGCTCGCCCGGCTCGCGAAGATCTGCCACAACGTGATGCT
GGGCGTGGTCACCCAATGCCTGGCGGAGATCACCGTGCTCGCCGAGAAGGGCGGGATGTCGCGCGCCGCGTTCCTGGAGT
TCCTCAACAAGTCGGTGATGGGCTCGGTGTTCACCACCTACAAGACCCCGGCGTTCGTGAACCTCGACTACACCCCGACG
TTCACGCCGATCCTGCTGCGCAAGGACTTCGACCTCGGCCTCGCGGCCGCCCGCCAGCTCGACGTCCCGATGCCGGTCGC
CGCGGCCACGAACGCCGCCGTCCAGGCCAGCGTGAGCAGCGGCCGGGTCGAGGAGGACTTCGCGATCCTGCTCGACCTCC
AGGCGCACAACTCCGGCATCACCCTCAAGGCCGAGAACGTCCAGGTCGACGACGGGCTCGGCGGAGAGCTGGGCTGA

Upstream 100 bases:

>100_bases
ACGGCTGCGCCAACGTCGCGCCCTGGCACCGGCCCCGCACCGCCGACTGGCTGGCCACCCGGCTCGCCGTACCCGCACTA
CTGACGAAGGCAGGATCATG

Downstream 100 bases:

>100_bases
TGAGCGCCCCGAACCGACCGCTCCCGGCGCCCGGGCACATGGCGGTCGACTACGAGGAGCGGGTCGACTTCGACCGGCTG
CGCCACTACCGGCTCGGCCG

Product: 6-phosphogluconate dehydrogenase

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MDTTVPQPGSLTVGWIGAGRMGAAMASRLARAGEDVTVWNRTRAKAEALSEVGCGVADTIADLRGHDVVFTMVSTPADLE
QVLVGEGGLLADPARVPAVVVDCSTVSTESSAAMRAACSERGVAFLAAPVSGNGKVVRAGGLSLVVSGPEETYERVAHLL
DHLGKGATYVGEGELARLAKICHNVMLGVVTQCLAEITVLAEKGGMSRAAFLEFLNKSVMGSVFTTYKTPAFVNLDYTPT
FTPILLRKDFDLGLAAARQLDVPMPVAAATNAAVQASVSSGRVEEDFAILLDLQAHNSGITLKAENVQVDDGLGGELG

Sequences:

>Translated_318_residues
MDTTVPQPGSLTVGWIGAGRMGAAMASRLARAGEDVTVWNRTRAKAEALSEVGCGVADTIADLRGHDVVFTMVSTPADLE
QVLVGEGGLLADPARVPAVVVDCSTVSTESSAAMRAACSERGVAFLAAPVSGNGKVVRAGGLSLVVSGPEETYERVAHLL
DHLGKGATYVGEGELARLAKICHNVMLGVVTQCLAEITVLAEKGGMSRAAFLEFLNKSVMGSVFTTYKTPAFVNLDYTPT
FTPILLRKDFDLGLAAARQLDVPMPVAAATNAAVQASVSSGRVEEDFAILLDLQAHNSGITLKAENVQVDDGLGGELG
>Mature_318_residues
MDTTVPQPGSLTVGWIGAGRMGAAMASRLARAGEDVTVWNRTRAKAEALSEVGCGVADTIADLRGHDVVFTMVSTPADLE
QVLVGEGGLLADPARVPAVVVDCSTVSTESSAAMRAACSERGVAFLAAPVSGNGKVVRAGGLSLVVSGPEETYERVAHLL
DHLGKGATYVGEGELARLAKICHNVMLGVVTQCLAEITVLAEKGGMSRAAFLEFLNKSVMGSVFTTYKTPAFVNLDYTPT
FTPILLRKDFDLGLAAARQLDVPMPVAAATNAAVQASVSSGRVEEDFAILLDLQAHNSGITLKAENVQVDDGLGGELG

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=291, Percent_Identity=28.8659793814433, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=24.2214532871972, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1786719, Length=291, Percent_Identity=27.8350515463918, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=25.3521126760563, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=25.7627118644068, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789092, Length=296, Percent_Identity=29.0540540540541, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557316, Length=302, Percent_Identity=25.8278145695364, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=27.7966101694915, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=27.7966101694915, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=26.0714285714286, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 32970; Mature: 32970

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTVPQPGSLTVGWIGAGRMGAAMASRLARAGEDVTVWNRTRAKAEALSEVGCGVADTI
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCHHHHH
ADLRGHDVVFTMVSTPADLEQVLVGEGGLLADPARVPAVVVDCSTVSTESSAAMRAACSE
HHCCCCEEEEEEECCCCHHHHHHHCCCCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHH
RGVAFLAAPVSGNGKVVRAGGLSLVVSGPEETYERVAHLLDHLGKGATYVGEGELARLAK
CCEEEEEEEECCCCCEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHH
ICHNVMLGVVTQCLAEITVLAEKGGMSRAAFLEFLNKSVMGSVFTTYKTPAFVNLDYTPT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
FTPILLRKDFDLGLAAARQLDVPMPVAAATNAAVQASVSSGRVEEDFAILLDLQAHNSGI
CCEEEEECCCCCHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCE
TLKAENVQVDDGLGGELG
EEEECCEEECCCCCCCCC
>Mature Secondary Structure
MDTTVPQPGSLTVGWIGAGRMGAAMASRLARAGEDVTVWNRTRAKAEALSEVGCGVADTI
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCHHHHH
ADLRGHDVVFTMVSTPADLEQVLVGEGGLLADPARVPAVVVDCSTVSTESSAAMRAACSE
HHCCCCEEEEEEECCCCHHHHHHHCCCCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHH
RGVAFLAAPVSGNGKVVRAGGLSLVVSGPEETYERVAHLLDHLGKGATYVGEGELARLAK
CCEEEEEEEECCCCCEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHH
ICHNVMLGVVTQCLAEITVLAEKGGMSRAAFLEFLNKSVMGSVFTTYKTPAFVNLDYTPT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
FTPILLRKDFDLGLAAARQLDVPMPVAAATNAAVQASVSSGRVEEDFAILLDLQAHNSGI
CCEEEEECCCCCHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCE
TLKAENVQVDDGLGGELG
EEEECCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]