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The map label for this gene is livJ [C]

Identifier: 119718662

GI number: 119718662

Start: 4696833

End: 4698074

Strand: Reverse

Name: livJ [C]

Synonym: Noca_4443

Alternate gene names: 119718662

Gene position: 4698074-4696833 (Counterclockwise)

Preceding gene: 119718669

Following gene: 119718661

Centisome position: 94.23

GC content: 68.04

Gene sequence:

>1242_bases
ATGTCGCACGTTTCGCTTCGCCAGGCTTGGACAGCCGTGGCCCTGACCGGAGTGCTGGCGCTCGGACTGGCAGCCTGCGG
TGGTGACGACTCGGAAGGCGGCGGTGGCGACAGCTCCGGCCCGATCCGGATCGGCTCCTCGCTCCCGCTGACCGGCGAGT
TCTCCCAGCCCGGCCAGGCGGCAAAGCAGGGCTACGAGGTCTGGCAGGAGATGGTCAACGATGCCGGCGGCCTCCTGGGC
CGGGAGGTCGAGCTCGTCGTCAAGGACGACGCCTCGAACCAGAACACCGTGGTCACCGACTACAACGCGCTGATCAGCCA
GGACAAGGTCGACCTCCTTCTCGGGACCTTCTCCTCGCTGCTCAACCTGCCCGCCTCGGCGGTCGCGGAGAAGAACCAGA
TGCTGTACGTCGAGCCCGCGGGCGGCTCGCCCGACATGTTCAACCGCGGCTTCAAGTACCTCTTCTTCACCCAGCAGGCG
ACCTCGAACCTGCAGGGCAAGGTGTTCGCCGAGTGGGTCGCCGCCCTCCCGGAGGACCAGCGGCCGAAGACCGCGGCGTA
CCCGACCCTCGACGACCCGTTCGCCGCGCCCAACGTGGCCGGCATCCAGGAGATCCTCGAGGCCGCGGGCGTGGAGACGG
TCTACCAGGAGACCTACGCGATCGACACCAAGAACTTCGACACGATCGTCAACGCCATGAAGAACGCGGACCCCGACCTG
GTCGTGCACGGTGCGCAGTTCGAGGACGGCATCGGCCTGACCCGCTCGATGCTCAAGGCCGACTTCACCCCTGGCATGTT
CTACGAGACCAACGCCCCGTCCTTCGGTGCGCAGTACAGCGACGGCATCGGCATGGAGAACACCGAGGGCGTCATCTACG
CGGTCAGCCACTCGCCTGAGGCCGACACCCCCGGCAACGCCGAGTTCGTCGCCAAGTACAAGGAGATGTTCGGCACCGAC
GAGGTCCCCGAGGACGCCGCCGACGGCTACGCCGCGGCCCAGGTGATGCAGGCGGCCGTCGAGGCCGTGGGCAACATCGA
CGACCAGGAGGCGATGGCGAGCTGGCTGCGCGAGAACGAGGTCGACACGATTCTGGGCTCGCTGTCCTGGGACGAGACCG
GCGCGCCGACCGGTGAGTTCCTGATCGGCCAGTGGCAGAACGGCAAGCCCGAGATCGTGCTGCCGGCCGACGTGTCCACC
GCGGACATCGAGCCCGGCTGGCACCCGGGGGCCGGTGGCTGA

Upstream 100 bases:

>100_bases
CATATGCGGGGAAATGCTTGACAGACTGTGGCGCCCTTCACAAGACTGCGGATGCCGCAATCGATTGCGACAATCATGAG
TGCAGCACCGAGGAGGAAGA

Downstream 100 bases:

>100_bases
TGACCGAGCTGCTCCAGAACCTCCTCCTCGGCCTGCTGCTGGGCGGCGTGTACGCCCTCGCGGCGAGTGGGTTGACCCTG
ATCTTCGGTGTCATGCGGGT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSHVSLRQAWTAVALTGVLALGLAACGGDDSEGGGGDSSGPIRIGSSLPLTGEFSQPGQAAKQGYEVWQEMVNDAGGLLG
REVELVVKDDASNQNTVVTDYNALISQDKVDLLLGTFSSLLNLPASAVAEKNQMLYVEPAGGSPDMFNRGFKYLFFTQQA
TSNLQGKVFAEWVAALPEDQRPKTAAYPTLDDPFAAPNVAGIQEILEAAGVETVYQETYAIDTKNFDTIVNAMKNADPDL
VVHGAQFEDGIGLTRSMLKADFTPGMFYETNAPSFGAQYSDGIGMENTEGVIYAVSHSPEADTPGNAEFVAKYKEMFGTD
EVPEDAADGYAAAQVMQAAVEAVGNIDDQEAMASWLRENEVDTILGSLSWDETGAPTGEFLIGQWQNGKPEIVLPADVST
ADIEPGWHPGAGG

Sequences:

>Translated_413_residues
MSHVSLRQAWTAVALTGVLALGLAACGGDDSEGGGGDSSGPIRIGSSLPLTGEFSQPGQAAKQGYEVWQEMVNDAGGLLG
REVELVVKDDASNQNTVVTDYNALISQDKVDLLLGTFSSLLNLPASAVAEKNQMLYVEPAGGSPDMFNRGFKYLFFTQQA
TSNLQGKVFAEWVAALPEDQRPKTAAYPTLDDPFAAPNVAGIQEILEAAGVETVYQETYAIDTKNFDTIVNAMKNADPDL
VVHGAQFEDGIGLTRSMLKADFTPGMFYETNAPSFGAQYSDGIGMENTEGVIYAVSHSPEADTPGNAEFVAKYKEMFGTD
EVPEDAADGYAAAQVMQAAVEAVGNIDDQEAMASWLRENEVDTILGSLSWDETGAPTGEFLIGQWQNGKPEIVLPADVST
ADIEPGWHPGAGG
>Mature_412_residues
SHVSLRQAWTAVALTGVLALGLAACGGDDSEGGGGDSSGPIRIGSSLPLTGEFSQPGQAAKQGYEVWQEMVNDAGGLLGR
EVELVVKDDASNQNTVVTDYNALISQDKVDLLLGTFSSLLNLPASAVAEKNQMLYVEPAGGSPDMFNRGFKYLFFTQQAT
SNLQGKVFAEWVAALPEDQRPKTAAYPTLDDPFAAPNVAGIQEILEAAGVETVYQETYAIDTKNFDTIVNAMKNADPDLV
VHGAQFEDGIGLTRSMLKADFTPGMFYETNAPSFGAQYSDGIGMENTEGVIYAVSHSPEADTPGNAEFVAKYKEMFGTDE
VPEDAADGYAAAQVMQAAVEAVGNIDDQEAMASWLRENEVDTILGSLSWDETGAPTGEFLIGQWQNGKPEIVLPADVSTA
DIEPGWHPGAGG

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=350, Percent_Identity=25.7142857142857, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789867, Length=390, Percent_Identity=24.6153846153846, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43756; Mature: 43624

Theoretical pI: Translated: 3.82; Mature: 3.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVSLRQAWTAVALTGVLALGLAACGGDDSEGGGGDSSGPIRIGSSLPLTGEFSQPGQA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
AKQGYEVWQEMVNDAGGLLGREVELVVKDDASNQNTVVTDYNALISQDKVDLLLGTFSSL
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECHHHHHCCCHHHHHHHHHHHH
LNLPASAVAEKNQMLYVEPAGGSPDMFNRGFKYLFFTQQATSNLQGKVFAEWVAALPEDQ
HCCCHHHHHCCCCEEEEECCCCCCHHHHCCCEEEEEECHHHCCCCCHHHHHHHHHCCCCC
RPKTAAYPTLDDPFAAPNVAGIQEILEAAGVETVYQETYAIDTKNFDTIVNAMKNADPDL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHEECCCCHHHHHHHHHCCCCCE
VVHGAQFEDGIGLTRSMLKADFTPGMFYETNAPSFGAQYSDGIGMENTEGVIYAVSHSPE
EEECCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
ADTPGNAEFVAKYKEMFGTDEVPEDAADGYAAAQVMQAAVEAVGNIDDQEAMASWLRENE
CCCCCCHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
VDTILGSLSWDETGAPTGEFLIGQWQNGKPEIVLPADVSTADIEPGWHPGAGG
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SHVSLRQAWTAVALTGVLALGLAACGGDDSEGGGGDSSGPIRIGSSLPLTGEFSQPGQA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
AKQGYEVWQEMVNDAGGLLGREVELVVKDDASNQNTVVTDYNALISQDKVDLLLGTFSSL
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECHHHHHCCCHHHHHHHHHHHH
LNLPASAVAEKNQMLYVEPAGGSPDMFNRGFKYLFFTQQATSNLQGKVFAEWVAALPEDQ
HCCCHHHHHCCCCEEEEECCCCCCHHHHCCCEEEEEECHHHCCCCCHHHHHHHHHCCCCC
RPKTAAYPTLDDPFAAPNVAGIQEILEAAGVETVYQETYAIDTKNFDTIVNAMKNADPDL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHEECCCCHHHHHHHHHCCCCCE
VVHGAQFEDGIGLTRSMLKADFTPGMFYETNAPSFGAQYSDGIGMENTEGVIYAVSHSPE
EEECCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
ADTPGNAEFVAKYKEMFGTDEVPEDAADGYAAAQVMQAAVEAVGNIDDQEAMASWLRENE
CCCCCCHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
VDTILGSLSWDETGAPTGEFLIGQWQNGKPEIVLPADVSTADIEPGWHPGAGG
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA