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The map label for this gene is purF [H]

Identifier: 119718535

GI number: 119718535

Start: 4563229

End: 4564773

Strand: Reverse

Name: purF [H]

Synonym: Noca_4316

Alternate gene names: 119718535

Gene position: 4564773-4563229 (Counterclockwise)

Preceding gene: 119718538

Following gene: 119718534

Centisome position: 91.55

GC content: 71.39

Gene sequence:

>1545_bases
GTGCCCTACCAAAGCAGCCCCCGCAAGGGAGGCGACGGCAGGCTGACCGCGGCGCTCGACCCCCAGGACCAGGGCCCCCA
GGACGCCTGCGGCGTCTTCGGCGTCTGGGCACCCGGCGAGGACGTCGCCAAGCTCACCTACTTCGGCCTGTACGCGCTCC
AGCACCGCGGCCAGGAGTCGGCGGGCATCGCGGTCAGCAACGGCCGCCAGATCCTGGTCTACAAGGACATGGGCCTGGTC
TCCCAGGTCTTCGACGAGACCACCCTGGCCTCGCTCAAGGGGCACCTGGCCATCGGCCACAGCCGCTACTCCACCACCGG
GGCGAGCACCTGGCAGAACGCGCAGCCGACCTTCCGGCCCACCGCGGACGGCTCGATCGCGCTCGGCCACAACGGCAACC
TGATCAACACCCACGACCTGGCCCGGATGGTGGTCGACCTGCCCGGCCCCGGCGACGAGCTCGAGCTGCACACCCGCCCG
GCCGAGACGTCGACCAACGACACCGGCCTGGTGACCGCGCTCCTGGCGCACCACCCCGACACGTCGCTGGAGCAGCGCGC
GCTGGAGGTGCTGCCCCAGCTGAAGGGCGCCTTCTGCTTCGTCTGGATGAACGAGGACACCCTGTACGCCGCCCGCGACG
CCCAGGGCATCCGGCCGCTCGTGCTCGGCCGGCTCGAGCGCGGCTGGGTGGTCGCCAGCGAGGACGCCGCACTGGCCACC
ATCGGCGCGAGCGTGGTCCGCGAGGTCGAGCCCGGCGAGATGATCGTCATCGACGAGTTCGGCCTGCGCTCACACAAGTT
CGCCGACCCGGCGCCGAAGGGCTGCGTCTTCGAGTACGTCTACCTGGCCCGGCCGGACGCCACGATCAGCGGCCGCAGCG
TCCACGAGTCCCGGGTCGAGATGGGCCGCCAGCTCGCCCGCGAGTACCCCGTCGACGCCGACCTGGTGATCCCGGTCCCC
GAGTCCGGCACCCCCGCGGCCGCCGGCTACGCCGAGGAGAGCGGCATCCCGTTCGGCCAGGGTTTCGTGAAGAACGCGTA
CGTCGGGCGCACCTTCATCCAGCCCAGCCAGACGCTGCGCCAGCTCGGCATCCGCCTCAAGCTCAACGCCCTCGAGCACA
TGATCCGCGGCAAGCGGATCGTCGTCGTCGACGACTCGATCGTGCGCGGCAACACCCAGCGCGCCCAGGTCCGGATGCTC
CGCGAGGCCGGGGCCCGCGAGGTCCACGTGCGGATCTCCAGCCCGCCGGTGAAGTGGCCGTGCTTCTACGGCATCGACTT
CGCGACCCGCGCCGAGCTGATCGCCAACGGCCTGACCCCCGAGGAGATCGCGGCCAGCATCGGCGCCGACAGCCTCGGCT
ACATCTCCCTCGACGGCATGATCGACGCGACCGGCCTGGAGCCGGACCGGCTCTGCCGGGCCTGCTTCACCGGCGAGTAC
CCGATCCCGCTCCCCGACGAGAGCCTGCTCGGCAAGCACCTGCTCGAGTCCTCGATCCAGGCGCCGTCGTACGGCAAGGC
GCTGCCCGTCCTCAACAACCCGTAG

Upstream 100 bases:

>100_bases
GGTCGCGGAGCGGTCCTGGGCTGGTTTCGCACTCCTCCCCGACTCCTCAACGGTGGTACGGCAGATGGGACACGGGGGAG
GGAGGCCCTAAACTCGGCAC

Downstream 100 bases:

>100_bases
GAGCCCCGTGTCCGAGCCCGTTCCTGGCAACGCCTACGCCGCCGCCGGCGTGTCCATCGAGGCCGGTGACAAGGCCGTCG
AGCTGATGAAGGTGTGGGTC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MPYQSSPRKGGDGRLTAALDPQDQGPQDACGVFGVWAPGEDVAKLTYFGLYALQHRGQESAGIAVSNGRQILVYKDMGLV
SQVFDETTLASLKGHLAIGHSRYSTTGASTWQNAQPTFRPTADGSIALGHNGNLINTHDLARMVVDLPGPGDELELHTRP
AETSTNDTGLVTALLAHHPDTSLEQRALEVLPQLKGAFCFVWMNEDTLYAARDAQGIRPLVLGRLERGWVVASEDAALAT
IGASVVREVEPGEMIVIDEFGLRSHKFADPAPKGCVFEYVYLARPDATISGRSVHESRVEMGRQLAREYPVDADLVIPVP
ESGTPAAAGYAEESGIPFGQGFVKNAYVGRTFIQPSQTLRQLGIRLKLNALEHMIRGKRIVVVDDSIVRGNTQRAQVRML
REAGAREVHVRISSPPVKWPCFYGIDFATRAELIANGLTPEEIAASIGADSLGYISLDGMIDATGLEPDRLCRACFTGEY
PIPLPDESLLGKHLLESSIQAPSYGKALPVLNNP

Sequences:

>Translated_514_residues
MPYQSSPRKGGDGRLTAALDPQDQGPQDACGVFGVWAPGEDVAKLTYFGLYALQHRGQESAGIAVSNGRQILVYKDMGLV
SQVFDETTLASLKGHLAIGHSRYSTTGASTWQNAQPTFRPTADGSIALGHNGNLINTHDLARMVVDLPGPGDELELHTRP
AETSTNDTGLVTALLAHHPDTSLEQRALEVLPQLKGAFCFVWMNEDTLYAARDAQGIRPLVLGRLERGWVVASEDAALAT
IGASVVREVEPGEMIVIDEFGLRSHKFADPAPKGCVFEYVYLARPDATISGRSVHESRVEMGRQLAREYPVDADLVIPVP
ESGTPAAAGYAEESGIPFGQGFVKNAYVGRTFIQPSQTLRQLGIRLKLNALEHMIRGKRIVVVDDSIVRGNTQRAQVRML
REAGAREVHVRISSPPVKWPCFYGIDFATRAELIANGLTPEEIAASIGADSLGYISLDGMIDATGLEPDRLCRACFTGEY
PIPLPDESLLGKHLLESSIQAPSYGKALPVLNNP
>Mature_513_residues
PYQSSPRKGGDGRLTAALDPQDQGPQDACGVFGVWAPGEDVAKLTYFGLYALQHRGQESAGIAVSNGRQILVYKDMGLVS
QVFDETTLASLKGHLAIGHSRYSTTGASTWQNAQPTFRPTADGSIALGHNGNLINTHDLARMVVDLPGPGDELELHTRPA
ETSTNDTGLVTALLAHHPDTSLEQRALEVLPQLKGAFCFVWMNEDTLYAARDAQGIRPLVLGRLERGWVVASEDAALATI
GASVVREVEPGEMIVIDEFGLRSHKFADPAPKGCVFEYVYLARPDATISGRSVHESRVEMGRQLAREYPVDADLVIPVPE
SGTPAAAGYAEESGIPFGQGFVKNAYVGRTFIQPSQTLRQLGIRLKLNALEHMIRGKRIVVVDDSIVRGNTQRAQVRMLR
EAGAREVHVRISSPPVKWPCFYGIDFATRAELIANGLTPEEIAASIGADSLGYISLDGMIDATGLEPDRLCRACFTGEYP
IPLPDESLLGKHLLESSIQAPSYGKALPVLNNP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=526, Percent_Identity=39.7338403041825, Blast_Score=360, Evalue=2e-99,
Organism=Escherichia coli, GI1788651, Length=488, Percent_Identity=39.344262295082, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1790167, Length=219, Percent_Identity=30.5936073059361, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=489, Percent_Identity=38.241308793456, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6323958, Length=486, Percent_Identity=34.156378600823, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24659598, Length=498, Percent_Identity=45.1807228915663, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI28573187, Length=508, Percent_Identity=44.2913385826772, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=45.397489539749, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55540; Mature: 55408

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYQSSPRKGGDGRLTAALDPQDQGPQDACGVFGVWAPGEDVAKLTYFGLYALQHRGQES
CCCCCCCCCCCCCEEEEEECCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
AGIAVSNGRQILVYKDMGLVSQVFDETTLASLKGHLAIGHSRYSTTGASTWQNAQPTFRP
CCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCC
TADGSIALGHNGNLINTHDLARMVVDLPGPGDELELHTRPAETSTNDTGLVTALLAHHPD
CCCCCEEECCCCCEECHHHHEEHEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCC
TSLEQRALEVLPQLKGAFCFVWMNEDTLYAARDAQGIRPLVLGRLERGWVVASEDAALAT
CHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCHHHHHCCCCCEEEECCCCHHHH
IGASVVREVEPGEMIVIDEFGLRSHKFADPAPKGCVFEYVYLARPDATISGRSVHESRVE
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHH
MGRQLAREYPVDADLVIPVPESGTPAAAGYAEESGIPFGQGFVKNAYVGRTFIQPSQTLR
HHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHCCCCCCCCHHHCCCCCCEECCHHHHHH
QLGIRLKLNALEHMIRGKRIVVVDDSIVRGNTQRAQVRMLREAGAREVHVRISSPPVKWP
HHCCEEEEHHHHHHHCCCEEEEECCHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
CFYGIDFATRAELIANGLTPEEIAASIGADSLGYISLDGMIDATGLEPDRLCRACFTGEY
EEECCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCEEECCCCCHHHHHHHHHCCCC
PIPLPDESLLGKHLLESSIQAPSYGKALPVLNNP
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PYQSSPRKGGDGRLTAALDPQDQGPQDACGVFGVWAPGEDVAKLTYFGLYALQHRGQES
CCCCCCCCCCCCEEEEEECCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
AGIAVSNGRQILVYKDMGLVSQVFDETTLASLKGHLAIGHSRYSTTGASTWQNAQPTFRP
CCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCC
TADGSIALGHNGNLINTHDLARMVVDLPGPGDELELHTRPAETSTNDTGLVTALLAHHPD
CCCCCEEECCCCCEECHHHHEEHEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCC
TSLEQRALEVLPQLKGAFCFVWMNEDTLYAARDAQGIRPLVLGRLERGWVVASEDAALAT
CHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCHHHHHCCCCCEEEECCCCHHHH
IGASVVREVEPGEMIVIDEFGLRSHKFADPAPKGCVFEYVYLARPDATISGRSVHESRVE
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHH
MGRQLAREYPVDADLVIPVPESGTPAAAGYAEESGIPFGQGFVKNAYVGRTFIQPSQTLR
HHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHCCCCCCCCHHHCCCCCCEECCHHHHHH
QLGIRLKLNALEHMIRGKRIVVVDDSIVRGNTQRAQVRMLREAGAREVHVRISSPPVKWP
HHCCEEEEHHHHHHHCCCEEEEECCHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
CFYGIDFATRAELIANGLTPEEIAASIGADSLGYISLDGMIDATGLEPDRLCRACFTGEY
EEECCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCEEECCCCCHHHHHHHHHCCCC
PIPLPDESLLGKHLLESSIQAPSYGKALPVLNNP
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]