Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 119718162

GI number: 119718162

Start: 4152736

End: 4153884

Strand: Reverse

Name: mdeA [H]

Synonym: Noca_3943

Alternate gene names: 119718162

Gene position: 4153884-4152736 (Counterclockwise)

Preceding gene: 119718169

Following gene: 119718161

Centisome position: 83.31

GC content: 75.63

Gene sequence:

>1149_bases
ATGGACACCGACGCCGTTCACCTCGGCCGGGAGGACCTCGCCGCGCGCGGCGCACACGTGCCTCCACTCGATCTCTCCAC
GACCTACCCGCTGCCGAGCGTCGACGACGGCGGCGCGGCCTATGAGCGACTGGCCACCGGCGGCCGCCCCGAGGCGTCCG
ACTCGCTCGTCTACCAGCGGCTCTGGAACCCCAACGTCGACCGGTTCGAGCGCTCGGTCGCCGGCCTCGAGAGCCTGCCC
GGTGCGGTCGCGTTCGCCTCCGGCATGGCCGCGCTCAGCGCCGTGCTGGTCGCGACCGTCGCCGCGGGCCGCCCGCATGT
CGTCGGCGTGCGTCCGTTGTACGGCGGCACCGACCACGTGCTCGCGACCGGCCTGCTCGGGACCCGGGTCACCTGGGCCG
CGCCGGACCGGATCGCCGAGGCGATCGGCACCGACACCGGGCTGGTGGTCGTCGAGACCCCGGCCAACCCGACCGTGGAC
CTGGTCGACCTCACGGCGGTGGTCGCCCAGGCGGGCGACGTGCCGGTCCTGGTCGACAACACCTTCGCCACCCCGGTGCT
GCAGCGACCCGCCGCCCACGGCGCGGCCCTGGTGCTGCACTCCGCGACGAAGTTCATGGGCGGTCACGGTGACGTGATGG
GCGGCGTCGTCGCCGCGGGCGAGGAGTGGCAGGTCCGGCTGCGCCAGGTCCGCGCGCTGACCGGCGGGCTCCTGCACCCG
CGCGCGGCGTACGACCTGCACCGCGGCCTGCAGACCCTGCCGTTGCGGGTCCGGGCGCAGCAGGAGTCGGCGGCCGTGCT
CGCCGGCCGGCTCGCGGAGCACCCCTCGGTGGCGCGGGTGATGTACCCGGGCCTGAAGGAGTGCGACCCCACCGGCCTGG
TCGGCACCCAGATGAGCGGCCCCGGCAGCCTGCTGGCCTTCCAGCTCGCCGGAGGGTACGCCGCCGCCGCGGGCACCGCC
CAGCGGTGCCGGCTGATCACCCACGCGGTCTCGCTCGGCGGCGTCGACACGCTGATCCAGCACCCCGCAGGCCTGACCCA
CCGCCCCGTCGAGGCCGCCGCCCGTCCCGGTGAGGCCCTGCTCCGGCTGTCCGTGGGTCTCGAGGACGTCGAAGACCTGT
GGGCCGACCTGGAGCGCGCGCTGGGCTGA

Upstream 100 bases:

>100_bases
GGTTTCCGAAGATCCCTCGGCAGGACGGCTGCTTCACGAAGGATCTGCGCGACTGTTGCCCCCGAGATCACGCAGATCCA
CGCTGGAGGGCATGAGCCGC

Downstream 100 bases:

>100_bases
CTCCCCGGTGCCGCGGTGTCGATGGTTTGACCGGCGACACCCGGGGCATCTAGGACACTGAGCCCTGCCGGCTGCGCCGG
CGGGCGAACAGGTGGCCGAG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDTDAVHLGREDLAARGAHVPPLDLSTTYPLPSVDDGGAAYERLATGGRPEASDSLVYQRLWNPNVDRFERSVAGLESLP
GAVAFASGMAALSAVLVATVAAGRPHVVGVRPLYGGTDHVLATGLLGTRVTWAAPDRIAEAIGTDTGLVVVETPANPTVD
LVDLTAVVAQAGDVPVLVDNTFATPVLQRPAAHGAALVLHSATKFMGGHGDVMGGVVAAGEEWQVRLRQVRALTGGLLHP
RAAYDLHRGLQTLPLRVRAQQESAAVLAGRLAEHPSVARVMYPGLKECDPTGLVGTQMSGPGSLLAFQLAGGYAAAAGTA
QRCRLITHAVSLGGVDTLIQHPAGLTHRPVEAAARPGEALLRLSVGLEDVEDLWADLERALG

Sequences:

>Translated_382_residues
MDTDAVHLGREDLAARGAHVPPLDLSTTYPLPSVDDGGAAYERLATGGRPEASDSLVYQRLWNPNVDRFERSVAGLESLP
GAVAFASGMAALSAVLVATVAAGRPHVVGVRPLYGGTDHVLATGLLGTRVTWAAPDRIAEAIGTDTGLVVVETPANPTVD
LVDLTAVVAQAGDVPVLVDNTFATPVLQRPAAHGAALVLHSATKFMGGHGDVMGGVVAAGEEWQVRLRQVRALTGGLLHP
RAAYDLHRGLQTLPLRVRAQQESAAVLAGRLAEHPSVARVMYPGLKECDPTGLVGTQMSGPGSLLAFQLAGGYAAAAGTA
QRCRLITHAVSLGGVDTLIQHPAGLTHRPVEAAARPGEALLRLSVGLEDVEDLWADLERALG
>Mature_382_residues
MDTDAVHLGREDLAARGAHVPPLDLSTTYPLPSVDDGGAAYERLATGGRPEASDSLVYQRLWNPNVDRFERSVAGLESLP
GAVAFASGMAALSAVLVATVAAGRPHVVGVRPLYGGTDHVLATGLLGTRVTWAAPDRIAEAIGTDTGLVVVETPANPTVD
LVDLTAVVAQAGDVPVLVDNTFATPVLQRPAAHGAALVLHSATKFMGGHGDVMGGVVAAGEEWQVRLRQVRALTGGLLHP
RAAYDLHRGLQTLPLRVRAQQESAAVLAGRLAEHPSVARVMYPGLKECDPTGLVGTQMSGPGSLLAFQLAGGYAAAAGTA
QRCRLITHAVSLGGVDTLIQHPAGLTHRPVEAAARPGEALLRLSVGLEDVEDLWADLERALG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=379, Percent_Identity=32.7176781002639, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI299473759, Length=374, Percent_Identity=31.0160427807487, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=28.8409703504043, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790375, Length=400, Percent_Identity=34.5, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1789383, Length=396, Percent_Identity=30.3030303030303, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=381, Percent_Identity=33.5958005249344, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=382, Percent_Identity=32.7225130890052, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17538055, Length=382, Percent_Identity=31.9371727748691, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319307, Length=398, Percent_Identity=35.678391959799, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=427, Percent_Identity=26.6978922716628, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=380, Percent_Identity=30.2631578947368, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323333, Length=447, Percent_Identity=26.8456375838926, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321170, Length=262, Percent_Identity=27.8625954198473, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922554, Length=394, Percent_Identity=32.2335025380711, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 39585; Mature: 39585

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTDAVHLGREDLAARGAHVPPLDLSTTYPLPSVDDGGAAYERLATGGRPEASDSLVYQR
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHH
LWNPNVDRFERSVAGLESLPGAVAFASGMAALSAVLVATVAAGRPHVVGVRPLYGGTDHV
HCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LATGLLGTRVTWAAPDRIAEAIGTDTGLVVVETPANPTVDLVDLTAVVAQAGDVPVLVDN
HHHHHHHCCEEECCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECC
TFATPVLQRPAAHGAALVLHSATKFMGGHGDVMGGVVAAGEEWQVRLRQVRALTGGLLHP
CCCCHHHHCCCCCCCEEEEEHHHHHCCCCCCHHCCEEECCHHHHHHHHHHHHHHCCCCCC
RAAYDLHRGLQTLPLRVRAQQESAAVLAGRLAEHPSVARVMYPGLKECDPTGLVGTQMSG
HHHHHHHCCHHHCCEEEEECHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCC
PGSLLAFQLAGGYAAAAGTAQRCRLITHAVSLGGVDTLIQHPAGLTHRPVEAAARPGEAL
CCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHCCCCHHH
LRLSVGLEDVEDLWADLERALG
EEHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTDAVHLGREDLAARGAHVPPLDLSTTYPLPSVDDGGAAYERLATGGRPEASDSLVYQR
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHH
LWNPNVDRFERSVAGLESLPGAVAFASGMAALSAVLVATVAAGRPHVVGVRPLYGGTDHV
HCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LATGLLGTRVTWAAPDRIAEAIGTDTGLVVVETPANPTVDLVDLTAVVAQAGDVPVLVDN
HHHHHHHCCEEECCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECC
TFATPVLQRPAAHGAALVLHSATKFMGGHGDVMGGVVAAGEEWQVRLRQVRALTGGLLHP
CCCCHHHHCCCCCCCEEEEEHHHHHCCCCCCHHCCEEECCHHHHHHHHHHHHHHCCCCCC
RAAYDLHRGLQTLPLRVRAQQESAAVLAGRLAEHPSVARVMYPGLKECDPTGLVGTQMSG
HHHHHHHCCHHHCCEEEEECHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCC
PGSLLAFQLAGGYAAAAGTAQRCRLITHAVSLGGVDTLIQHPAGLTHRPVEAAARPGEAL
CCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHCCCCHHH
LRLSVGLEDVEDLWADLERALG
EEHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]