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The map label for this gene is tuf

Identifier: 119718142

GI number: 119718142

Start: 4128474

End: 4129667

Strand: Reverse

Name: tuf

Synonym: Noca_3922

Alternate gene names: 119718142

Gene position: 4129667-4128474 (Counterclockwise)

Preceding gene: 119718143

Following gene: 119718139

Centisome position: 82.83

GC content: 67.17

Gene sequence:

>1194_bases
GTGGCTAAGGCGAAGTTCGAGCGGACCAAGCCGCACGTCAACATCGGCACCATCGGTCACATCGACCACGGCAAGACGAC
GCTCACGGCGGCGATCACCAAGGTGCTGCACGACAAGTACCCGGACCTGAACCCCTTCACGCCGTTCGACGAGATCGACA
AGGCCCCTGAGGAGCGTCAGCGCGGTATCACCATCTCGATCGCGCACGTCGAGTACCAGACCGAGTCGCGGCACTACGCG
CACGTCGACTGCCCGGGTCACGCGGACTACATCAAGAACATGATCACCGGTGCCGCGCAGATGGACGGCGCGATCCTCGT
GGTCGCCGCCACCGACGGCCCGATGCCGCAGACCCGTGAGCACGTGCTGCTGGCCCGCCAGGTCGGCGTGCCCGCCCTGG
TCGTCGCGCTCAACAAGTGCGACATGGTCGACGACGAGGAGCTCATCGAGCTCGTCGAGATGGAGGTGCGCGAGCTCCTC
TCCGAGTACGAGTTCCCGGGCGACGACATCCCGGTCGTGCGCGTGGCGGCCTTCCCGGCCCTCAACGGCGACGCCAAGTG
GGGCGAGTCGGTCCTCGAGCTGATGAACGCGGTCGACGAGTACATCCCGACCCCGGAGCGCGACACCGAGAAGCCGTTCC
TGATGCCGGTCGAGGACGTCTTCACGATCACCGGTCGCGGCACGGTCATCACCGGCCGGATCGAGCGCGGCATCGTCAAG
GTCGGCGAGGAGGTCGAGATCCTCGGCATCCGCGAGGCCTCGCAGAAGAGCACCGTCACCGGTGTCGAGATGTTCCGCAA
GCTGCTCGACGAGGGCCAGGCCGGTGAGAACGTCGGTCTGCTGCTCCGCGGCACCAAGCGCGAGGACGTCGAGCGCGGCA
TGGTCGTCGCCAAGCCCGGCACCACGACCCCGCACACCAACTTCGAGGCCTCGGTCTACATCCTCTCGAAGGAGGAGGGC
GGCCGCCACACGCCGTTCTTCAACAACTACCGCCCGCAGTTCTACTTCCGGACCACGGACGTGACCGGCGTGGTGACCCT
CCCCGAGGGCACCGAGATGGTCATGCCGGGCGACAACACCGAGATGGCCGTCGAGCTGATCCAGCCGATCGCCATGGACG
AGGGTCTGCGCTTCGCGATCCGCGAGGGCGGCCGCACCGTCGGCGCCGGCCGGGTCACCAAGATCACGAAGTGA

Upstream 100 bases:

>100_bases
AAGGTGCGTGGCGAGTAGCCCCAGCGGCTGCAGTCCGGACCCTCGGCAGTACCACCAGCAAGTACGAGTCAAGTAACAAC
CCAACCAGGAGGAGCCCACA

Downstream 100 bases:

>100_bases
TCGCCTGATCCAAACGAAGGGCCCCGAAAGCTGCGCAGCAGCTTTCGGGGCCCTTTGCTTTGGATCAGGCATCCTGCGCG
CTTGTGAGCCGAGTCGGCGG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDKYPDLNPFTPFDEIDKAPEERQRGITISIAHVEYQTESRHYA
HVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPALVVALNKCDMVDDEELIELVEMEVRELL
SEYEFPGDDIPVVRVAAFPALNGDAKWGESVLELMNAVDEYIPTPERDTEKPFLMPVEDVFTITGRGTVITGRIERGIVK
VGEEVEILGIREASQKSTVTGVEMFRKLLDEGQAGENVGLLLRGTKREDVERGMVVAKPGTTTPHTNFEASVYILSKEEG
GRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMAVELIQPIAMDEGLRFAIREGGRTVGAGRVTKITK

Sequences:

>Translated_397_residues
MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDKYPDLNPFTPFDEIDKAPEERQRGITISIAHVEYQTESRHYA
HVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPALVVALNKCDMVDDEELIELVEMEVRELL
SEYEFPGDDIPVVRVAAFPALNGDAKWGESVLELMNAVDEYIPTPERDTEKPFLMPVEDVFTITGRGTVITGRIERGIVK
VGEEVEILGIREASQKSTVTGVEMFRKLLDEGQAGENVGLLLRGTKREDVERGMVVAKPGTTTPHTNFEASVYILSKEEG
GRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMAVELIQPIAMDEGLRFAIREGGRTVGAGRVTKITK
>Mature_396_residues
AKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDKYPDLNPFTPFDEIDKAPEERQRGITISIAHVEYQTESRHYAH
VDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPALVVALNKCDMVDDEELIELVEMEVRELLS
EYEFPGDDIPVVRVAAFPALNGDAKWGESVLELMNAVDEYIPTPERDTEKPFLMPVEDVFTITGRGTVITGRIERGIVKV
GEEVEILGIREASQKSTVTGVEMFRKLLDEGQAGENVGLLLRGTKREDVERGMVVAKPGTTTPHTNFEASVYILSKEEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTEMAVELIQPIAMDEGLRFAIREGGRTVGAGRVTKITK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.5989847715736, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.8390022675737, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.8390022675737, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=28.9887640449438, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=28.314606741573, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI54607086, Length=326, Percent_Identity=29.7546012269939, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI46094014, Length=353, Percent_Identity=28.6118980169972, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194018522, Length=345, Percent_Identity=28.4057971014493, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=28.2738095238095, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=28.2738095238095, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI4503507, Length=376, Percent_Identity=25.7978723404255, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI157426893, Length=243, Percent_Identity=26.7489711934156, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI82546879, Length=435, Percent_Identity=23.2183908045977, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI53729339, Length=234, Percent_Identity=31.1965811965812, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI53729337, Length=234, Percent_Identity=31.1965811965812, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=75.4430379746835, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=75.4430379746835, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=31.7507418397626, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789108, Length=329, Percent_Identity=30.0911854103343, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=31.6666666666667, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788922, Length=246, Percent_Identity=28.8617886178862, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=48.6284289276808, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=44.9197860962567, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=30.9576837416481, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=30.9576837416481, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=443, Percent_Identity=28.8939051918736, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=29.0322580645161, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=28.8636363636364, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=25.8849557522124, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=29.4545454545455, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=41.3793103448276, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=281, Percent_Identity=26.3345195729537, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17565438, Length=331, Percent_Identity=24.1691842900302, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=64.572864321608, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=28.9532293986637, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=28.9532293986637, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=357, Percent_Identity=25.7703081232493, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=28.2798833819242, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=372, Percent_Identity=25.2688172043011, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=28.4518828451883, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.5241730279898, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.5241730279898, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=48.8431876606684, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=29.438202247191, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=29.438202247191, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=29.438202247191, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=29.438202247191, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=29.0540540540541, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=29.0540540540541, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=28.5057471264368, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=29.7385620915033, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=28.1899109792285, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647048, Length=370, Percent_Identity=25.4054054054054, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647046, Length=370, Percent_Identity=25.4054054054054, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572034, Length=169, Percent_Identity=33.7278106508876, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_NOCSJ (A1SNN5)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_925107.1
- ProteinModelPortal:   A1SNN5
- SMR:   A1SNN5
- STRING:   A1SNN5
- GeneID:   4598057
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3922
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   A1SNN5
- ProtClustDB:   PRK00049
- BioCyc:   NSP35761:NOCA_3922-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43854; Mature: 43722

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDKYPDLNPFTPFDEIDKAPEERQ
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
RGITISIAHVEYQTESRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPALVVALNKCDMVDDEELIELVEMEVRELLSEYEFPGDDIPVVRVAAFPA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
LNGDAKWGESVLELMNAVDEYIPTPERDTEKPFLMPVEDVFTITGRGTVITGRIERGIVK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECHHHHHHHH
VGEEVEILGIREASQKSTVTGVEMFRKLLDEGQAGENVGLLLRGTKREDVERGMVVAKPG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCC
TTTPHTNFEASVYILSKEEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNT
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCH
EMAVELIQPIAMDEGLRFAIREGGRTVGAGRVTKITK
HHHHHHHHHHHHCCCCEEEECCCCCEECCCCEEEECC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDKYPDLNPFTPFDEIDKAPEERQ
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
RGITISIAHVEYQTESRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPALVVALNKCDMVDDEELIELVEMEVRELLSEYEFPGDDIPVVRVAAFPA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
LNGDAKWGESVLELMNAVDEYIPTPERDTEKPFLMPVEDVFTITGRGTVITGRIERGIVK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECHHHHHHHH
VGEEVEILGIREASQKSTVTGVEMFRKLLDEGQAGENVGLLLRGTKREDVERGMVVAKPG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCEEEECCC
TTTPHTNFEASVYILSKEEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNT
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCH
EMAVELIQPIAMDEGLRFAIREGGRTVGAGRVTKITK
HHHHHHHHHHHHCCCCEEEECCCCCEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA