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The map label for this gene is gltX

Identifier: 119717560

GI number: 119717560

Start: 3540928

End: 3542403

Strand: Reverse

Name: gltX

Synonym: Noca_3336

Alternate gene names: 119717560

Gene position: 3542403-3540928 (Counterclockwise)

Preceding gene: 119717561

Following gene: 119717559

Centisome position: 71.05

GC content: 70.39

Gene sequence:

>1476_bases
GTGAACAACCAGCCGAAGACCGTTCGCGTCCGGATGGCGCCCAGCCCGACCGGGAGCCCGCACGTCGGCCTGGTCCGCAC
CGCGCTCTTCAACTGGGCGTTCGCGCGGCACCACCACGGCACCTTCGTGTTCCGCATCGAGGACACCGACCGGGAGCGCA
GCACCCAGGAGTCCTACGACGCGATCATCGACCTGATGCGCTGGCTCGGCCTGGACTGGGACGAGGGCCCGGAGGTCGGC
GGGCCGCACGCGCCGTACTTCCAGAGCGAGCGCGGCGACATCTACCGCGACGCGCTGGCCCGCCTCGCGGAGTCGTCGTA
CACCTACGACTGCTTCTGCACGAACGAGGAGGTGGACGCGCGCCGCAAGGCCTCCGGCTCGAAGGTGATGGGGTACGACG
GCTTCTGCCGCGACCTGTCCACCGAGCAGCGCGCCGCGTTCGAGGCCGAGGGCCGGCGACCGGTCGTCCGGTTCCGGATG
CCCGACGGGTCGATCACCTGGCACGACCTGGTGCGCGGCGACGTCACGTTCGAGACCCGGTTCGTGCCCGACTACGCGCT
GTGCCGCGCCAACGGCGATCCGCTCTACACCCTGGTCAACCCCGTCGACGACGCGTACATGGAGATCACCCACGTGCTGC
GTGGCGAGGACCTGCTCTCCAGCACGCCGCGCCAGATCGCGCTGTACGCCGCGCTCGAGGAGCTCGGCATCGCGAAGGCG
ACGCCGGAGTTCGGCCACCTGCCCTACGTCATGGGCGAGGGCAACAAGAAGCTGTCCAAGCGGGACCCGGAGGCGCACGC
GCTGGCCTACCGCGACCAGGGCTTCCTGCCGGAGGGCCTGCTCAACTACCTGGCCCTGCTGGGCTGGGCGATCGCCGCCG
ACCGTGACGTCTTCTCGATGGAGGAGATGGTCGCGGCGTTCGACATCGCCGACGTGAACCCCAACCCCGCACGCTTCGAC
ATGAAGAAGGCCGAGGCGATCAACGCCGCGCACATGCGGCTGCTCTCCCTCGACGTCATGACCGAGCGGGTGCTCCCGTT
CCTCAAGGGCGCCGGCGTGCTGTCCGACCCGGTCAACCCCGCGGACACCCAGCTGCTCGAGCTGGCCATGCCGCTGGTGG
CCGAGCGGATCAACAAGCTCACCGAGTCCGTGGACATGCTCGGCTTCCTGTTCGTCGACGAGGAGTCGTTCACGCGGGAC
GAGGCCGACGCGGAGAAGCTGCTCAACGACGACGGGCGGGCCGTCGTACGCCGCGCCCTCGACGCGCTCGAGCGAGTCGG
GGAGTGGTCGACGGGCGCCATCCAGGACGCGCTGCAGGCCGAGCTCGTCGAGGCGATGGGGGTCAAGCCGCGCAACGCGT
TCGGTCCGGTCCGGGTCGCCGTGACCGGGCGCCGGGTCTCGCCGCCGCTGTTCGAGTCGATGGAGCTGCTCGGCCGCGAC
CGATCGCTGGCCCGGCTGCGGAGCGCTCTTGGCTGA

Upstream 100 bases:

>100_bases
CCCGAGGGCGTCGGCCCCATGGACGTCGGCGACGAGGTCGAGGTCTCGATCGCCGGACTCGGCACTCTCACGAACCCCGT
CAAGGCAAGGTGACCCAGCG

Downstream 100 bases:

>100_bases
GCACGGGGCGCAGCCGGCGGTCGAGCCGGCTCCGGTTCGGGCCCCGCTGCCGTACCACCTCGTCCACCGGGGCGGCCGCC
GCGGCTCGTGGCGCCCGGTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNNQPKTVRVRMAPSPTGSPHVGLVRTALFNWAFARHHHGTFVFRIEDTDRERSTQESYDAIIDLMRWLGLDWDEGPEVG
GPHAPYFQSERGDIYRDALARLAESSYTYDCFCTNEEVDARRKASGSKVMGYDGFCRDLSTEQRAAFEAEGRRPVVRFRM
PDGSITWHDLVRGDVTFETRFVPDYALCRANGDPLYTLVNPVDDAYMEITHVLRGEDLLSSTPRQIALYAALEELGIAKA
TPEFGHLPYVMGEGNKKLSKRDPEAHALAYRDQGFLPEGLLNYLALLGWAIAADRDVFSMEEMVAAFDIADVNPNPARFD
MKKAEAINAAHMRLLSLDVMTERVLPFLKGAGVLSDPVNPADTQLLELAMPLVAERINKLTESVDMLGFLFVDEESFTRD
EADAEKLLNDDGRAVVRRALDALERVGEWSTGAIQDALQAELVEAMGVKPRNAFGPVRVAVTGRRVSPPLFESMELLGRD
RSLARLRSALG

Sequences:

>Translated_491_residues
MNNQPKTVRVRMAPSPTGSPHVGLVRTALFNWAFARHHHGTFVFRIEDTDRERSTQESYDAIIDLMRWLGLDWDEGPEVG
GPHAPYFQSERGDIYRDALARLAESSYTYDCFCTNEEVDARRKASGSKVMGYDGFCRDLSTEQRAAFEAEGRRPVVRFRM
PDGSITWHDLVRGDVTFETRFVPDYALCRANGDPLYTLVNPVDDAYMEITHVLRGEDLLSSTPRQIALYAALEELGIAKA
TPEFGHLPYVMGEGNKKLSKRDPEAHALAYRDQGFLPEGLLNYLALLGWAIAADRDVFSMEEMVAAFDIADVNPNPARFD
MKKAEAINAAHMRLLSLDVMTERVLPFLKGAGVLSDPVNPADTQLLELAMPLVAERINKLTESVDMLGFLFVDEESFTRD
EADAEKLLNDDGRAVVRRALDALERVGEWSTGAIQDALQAELVEAMGVKPRNAFGPVRVAVTGRRVSPPLFESMELLGRD
RSLARLRSALG
>Mature_491_residues
MNNQPKTVRVRMAPSPTGSPHVGLVRTALFNWAFARHHHGTFVFRIEDTDRERSTQESYDAIIDLMRWLGLDWDEGPEVG
GPHAPYFQSERGDIYRDALARLAESSYTYDCFCTNEEVDARRKASGSKVMGYDGFCRDLSTEQRAAFEAEGRRPVVRFRM
PDGSITWHDLVRGDVTFETRFVPDYALCRANGDPLYTLVNPVDDAYMEITHVLRGEDLLSSTPRQIALYAALEELGIAKA
TPEFGHLPYVMGEGNKKLSKRDPEAHALAYRDQGFLPEGLLNYLALLGWAIAADRDVFSMEEMVAAFDIADVNPNPARFD
MKKAEAINAAHMRLLSLDVMTERVLPFLKGAGVLSDPVNPADTQLLELAMPLVAERINKLTESVDMLGFLFVDEESFTRD
EADAEKLLNDDGRAVVRRALDALERVGEWSTGAIQDALQAELVEAMGVKPRNAFGPVRVAVTGRRVSPPLFESMELLGRD
RSLARLRSALG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=32.0328542094456, Blast_Score=232, Evalue=7e-61,
Organism=Escherichia coli, GI1788743, Length=490, Percent_Identity=37.3469387755102, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI226510925, Length=303, Percent_Identity=32.013201320132, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=489, Percent_Identity=31.9018404907975, Blast_Score=232, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6324540, Length=508, Percent_Identity=31.496062992126, Blast_Score=219, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6324742, Length=143, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=496, Percent_Identity=30.8467741935484, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_NOCSJ (A1SM03)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924525.1
- ProteinModelPortal:   A1SM03
- SMR:   A1SM03
- STRING:   A1SM03
- GeneID:   4600230
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3336
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- BioCyc:   NSP35761:NOCA_3336-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54815; Mature: 54815

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQPKTVRVRMAPSPTGSPHVGLVRTALFNWAFARHHHGTFVFRIEDTDRERSTQESYD
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHHHH
AIIDLMRWLGLDWDEGPEVGGPHAPYFQSERGDIYRDALARLAESSYTYDCFCTNEEVDA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHH
RRKASGSKVMGYDGFCRDLSTEQRAAFEAEGRRPVVRFRMPDGSITWHDLVRGDVTFETR
HHHCCCCEEECCCHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEE
FVPDYALCRANGDPLYTLVNPVDDAYMEITHVLRGEDLLSSTPRQIALYAALEELGIAKA
ECCCHHEEECCCCEEEEEECCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCCC
TPEFGHLPYVMGEGNKKLSKRDPEAHALAYRDQGFLPEGLLNYLALLGWAIAADRDVFSM
CCCCCCCCEEECCCCCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
EEMVAAFDIADVNPNPARFDMKKAEAINAAHMRLLSLDVMTERVLPFLKGAGVLSDPVNP
HHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADTQLLELAMPLVAERINKLTESVDMLGFLFVDEESFTRDEADAEKLLNDDGRAVVRRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHH
DALERVGEWSTGAIQDALQAELVEAMGVKPRNAFGPVRVAVTGRRVSPPLFESMELLGRD
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
RSLARLRSALG
HHHHHHHHHCC
>Mature Secondary Structure
MNNQPKTVRVRMAPSPTGSPHVGLVRTALFNWAFARHHHGTFVFRIEDTDRERSTQESYD
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHHHH
AIIDLMRWLGLDWDEGPEVGGPHAPYFQSERGDIYRDALARLAESSYTYDCFCTNEEVDA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHH
RRKASGSKVMGYDGFCRDLSTEQRAAFEAEGRRPVVRFRMPDGSITWHDLVRGDVTFETR
HHHCCCCEEECCCHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEE
FVPDYALCRANGDPLYTLVNPVDDAYMEITHVLRGEDLLSSTPRQIALYAALEELGIAKA
ECCCHHEEECCCCEEEEEECCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCCC
TPEFGHLPYVMGEGNKKLSKRDPEAHALAYRDQGFLPEGLLNYLALLGWAIAADRDVFSM
CCCCCCCCEEECCCCCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
EEMVAAFDIADVNPNPARFDMKKAEAINAAHMRLLSLDVMTERVLPFLKGAGVLSDPVNP
HHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ADTQLLELAMPLVAERINKLTESVDMLGFLFVDEESFTRDEADAEKLLNDDGRAVVRRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHH
DALERVGEWSTGAIQDALQAELVEAMGVKPRNAFGPVRVAVTGRRVSPPLFESMELLGRD
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
RSLARLRSALG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA