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The map label for this gene is dhaK [H]

Identifier: 119717540

GI number: 119717540

Start: 3523577

End: 3524578

Strand: Reverse

Name: dhaK [H]

Synonym: Noca_3316

Alternate gene names: 119717540

Gene position: 3524578-3523577 (Counterclockwise)

Preceding gene: 119717541

Following gene: 119717539

Centisome position: 70.69

GC content: 67.66

Gene sequence:

>1002_bases
ATGCGGAAGATCATCAACGCCCCGGAGAACTTCGTCGACGAGTTCGTCGAGGGGATCCTGCTGGCCCATCCGGACCTGGT
GAAGACGCCGGGGGACGACGTGCGAGTCATGGTGCGGGCCGATGCGCCGGTTGCCGGACAGGTCGGCATCGTCACCGGCG
GTGGCTCGGGTCACCTGCCGCTGTTCAAGGGGTACGTCGGTAGGGGGCTGTGCTCCGGTGTGGCGATCGGCAACGTGTTC
AGCTCACCCAGCTCTGACCAGATCTTCCAAGCGACCATGGCCGTCGACGGAGGTGCCGGCGTCCTCTACCTGTACGGCAA
CTACGGCGGAGACGTGCTCAACTTCGACCTCGCCGCTGATCTCGCCGACCTCGAGGGGGTCCCGACCATGACGGTCGTCG
GCCGGGACGACGTCGCAAGCCAGCCGAAGGACAACGAGCACGAGCGTCGGGGCGTCGCGGGCATCTTCTTCGCGTACAAG
GCCGCGGGCGCCGCTGCCGAGCGTGGAGACTCTCTCGAGCGGGTCGCCGCGATTGCCGAGGACGTTGTCGCGCACACCGC
GACCATCGGGATCGGTCTGGCGCCGACGATCCTGCCGACCACCGGCAAGCCCAGCTTCGAACTGCCGGACGGTCAGATGG
AGATCGGCATCGGCATCCACGGCGAGCCCGGGTCACGACGCGGTCCGTTGGAGACCGCCGACGAGATCGCGACCGACTTC
GTCGAGGCCCTCGTGGGTGACCTGTCGCTCCGTGAGGGGGCGCGGGTCGCGATCCTGGTCAACGGGCTCGGTGCGACGCC
GCTCGAGGAGCTCTACCTCCTCTACCGACGCGCCCACCAGCTCCTCGCCGAGCGCGGTATCGAGATTGTCAAGAGGTACG
TCGGCGAGTACGCCACGAGCCTCGAGATGGCTGGCGCGTCGATCTCGTTGCTGGAGCTCGATGACGCACGGCTGGAGCTG
CTGAACGCCCCGGCACTCTCCCCCTTCTTCCAGGAGCGCTGA

Upstream 100 bases:

>100_bases
TCGACCAGTTCAGCTTCAGGAAGCCGAGCCGACCCGTGCCCGTCGTGACCGACCCGCAACCATCCTCCACAGCGCCCGAC
AAGTGAACGGACCGAATGCC

Downstream 100 bases:

>100_bases
CCCGTGAACGCCAAGGACCTCAACGACCACATCCGGCGCAGCCTGGAGATCCTCACGGAGTCCGCGGACGAGCTGCGCGA
CCTCGACCAGGCGCTCGGGG

Product: Glycerone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRKIINAPENFVDEFVEGILLAHPDLVKTPGDDVRVMVRADAPVAGQVGIVTGGGSGHLPLFKGYVGRGLCSGVAIGNVF
SSPSSDQIFQATMAVDGGAGVLYLYGNYGGDVLNFDLAADLADLEGVPTMTVVGRDDVASQPKDNEHERRGVAGIFFAYK
AAGAAAERGDSLERVAAIAEDVVAHTATIGIGLAPTILPTTGKPSFELPDGQMEIGIGIHGEPGSRRGPLETADEIATDF
VEALVGDLSLREGARVAILVNGLGATPLEELYLLYRRAHQLLAERGIEIVKRYVGEYATSLEMAGASISLLELDDARLEL
LNAPALSPFFQER

Sequences:

>Translated_333_residues
MRKIINAPENFVDEFVEGILLAHPDLVKTPGDDVRVMVRADAPVAGQVGIVTGGGSGHLPLFKGYVGRGLCSGVAIGNVF
SSPSSDQIFQATMAVDGGAGVLYLYGNYGGDVLNFDLAADLADLEGVPTMTVVGRDDVASQPKDNEHERRGVAGIFFAYK
AAGAAAERGDSLERVAAIAEDVVAHTATIGIGLAPTILPTTGKPSFELPDGQMEIGIGIHGEPGSRRGPLETADEIATDF
VEALVGDLSLREGARVAILVNGLGATPLEELYLLYRRAHQLLAERGIEIVKRYVGEYATSLEMAGASISLLELDDARLEL
LNAPALSPFFQER
>Mature_333_residues
MRKIINAPENFVDEFVEGILLAHPDLVKTPGDDVRVMVRADAPVAGQVGIVTGGGSGHLPLFKGYVGRGLCSGVAIGNVF
SSPSSDQIFQATMAVDGGAGVLYLYGNYGGDVLNFDLAADLADLEGVPTMTVVGRDDVASQPKDNEHERRGVAGIFFAYK
AAGAAAERGDSLERVAAIAEDVVAHTATIGIGLAPTILPTTGKPSFELPDGQMEIGIGIHGEPGSRRGPLETADEIATDF
VEALVGDLSLREGARVAILVNGLGATPLEELYLLYRRAHQLLAERGIEIVKRYVGEYATSLEMAGASISLLELDDARLEL
LNAPALSPFFQER

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=331, Percent_Identity=35.9516616314199, Blast_Score=185, Evalue=5e-47,
Organism=Escherichia coli, GI87081857, Length=352, Percent_Identity=42.6136363636364, Blast_Score=267, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17565018, Length=327, Percent_Identity=36.085626911315, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321055, Length=326, Percent_Identity=33.1288343558282, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323570, Length=345, Percent_Identity=31.304347826087, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 35054; Mature: 35054

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIINAPENFVDEFVEGILLAHPDLVKTPGDDVRVMVRADAPVAGQVGIVTGGGSGHLP
CCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCH
LFKGYVGRGLCSGVAIGNVFSSPSSDQIFQATMAVDGGAGVLYLYGNYGGDVLNFDLAAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHH
LADLEGVPTMTVVGRDDVASQPKDNEHERRGVAGIFFAYKAAGAAAERGDSLERVAAIAE
HHHCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHH
DVVAHTATIGIGLAPTILPTTGKPSFELPDGQMEIGIGIHGEPGSRRGPLETADEIATDF
HHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
VEALVGDLSLREGARVAILVNGLGATPLEELYLLYRRAHQLLAERGIEIVKRYVGEYATS
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEMAGASISLLELDDARLELLNAPALSPFFQER
HHHCCCEEEEEEECCHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MRKIINAPENFVDEFVEGILLAHPDLVKTPGDDVRVMVRADAPVAGQVGIVTGGGSGHLP
CCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCH
LFKGYVGRGLCSGVAIGNVFSSPSSDQIFQATMAVDGGAGVLYLYGNYGGDVLNFDLAAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHH
LADLEGVPTMTVVGRDDVASQPKDNEHERRGVAGIFFAYKAAGAAAERGDSLERVAAIAE
HHHCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHH
DVVAHTATIGIGLAPTILPTTGKPSFELPDGQMEIGIGIHGEPGSRRGPLETADEIATDF
HHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
VEALVGDLSLREGARVAILVNGLGATPLEELYLLYRRAHQLLAERGIEIVKRYVGEYATS
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEMAGASISLLELDDARLELLNAPALSPFFQER
HHHCCCEEEEEEECCHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]