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The map label for this gene is ykoU [H]

Identifier: 119717080

GI number: 119717080

Start: 3029057

End: 3030424

Strand: Reverse

Name: ykoU [H]

Synonym: Noca_2856

Alternate gene names: 119717080

Gene position: 3030424-3029057 (Counterclockwise)

Preceding gene: 119717081

Following gene: 119717079

Centisome position: 60.78

GC content: 75.0

Gene sequence:

>1368_bases
GTGACCGGCAAGCTGGACGCCTACAGGCGCAAGCGCGACTTCACGCGTACGCCGGAGCCGGCGGGCGCGCCCGCGCCGGG
CGCCGGCACCGGCCGGTTCGTCGTCCAGCGGCACCGGGCCCGCCGGCTGCACTACGACCCGCGCGCCGCGGTCGAGGCGG
GGGAGCTGCACGCTGAGGTGCACGGCCGCAAGCTGCGCGGCCGCCTGGTCCTCGTCCGGCGCGACGGCGGGGGCGACAGT
GGGGGTGGCGACGACTGGATGCTGCTGCACAAGCACGACGAGCACGCCGTCGAGGGCTGGGACCCCGAGGACCACCCGGT
CTCGGTGGTCAGTGGACGCACCAACGACCAGGTGAAGGCCGACCCCGACCGGCTGTGGCGCTCCGACGCCCCCGCCGCGG
AGGCCAGCGTCTCGCTGCTCCCCGAGCCGCTGCCCGACGAGGCCATCGACGAGCTCGAGTCATTCGGCGAGCAGGGCACC
TGGGAGGTCTTCGGCCGGCGGCTCGAGGTGACCAACCTCGACAAGGTGCTGTTCCCCGCGCCGGCGGACGGGCCGCCGGT
CAGCAAGCGCGAGCTGCTCGCCTACGCCGCGCGCGTCGCGCCGGTGGCGCTGCCGTACCTGGAGGGCCGGGCGCTCAACA
TGCACCGGTATCCCGAGGGTGCCGACCGGCCCGGCTTCTGGCACAAGCAGCGGCCCGAGCACGCGCCCGAGTGGCTGACC
TGCTGGGAGAACCCGGACGCCGACGAGGGCGAGACCACGGCGTATGTCGTCCCCGACGAGCCGGCCGCCCTGGTGTGGGC
CGCGAACTTCGGGGCCCTCGAGTGGCACCCCTGGACCTCGCGCACGGGGCGGCCGCACGAGCCGACGTACGCCCTGATCG
ACCTCGATCCCGGGGAGCGGACCAGGTGGGCGGACCTGCTGGTGCTCGCCCGCCTGCACCGCACCGCGCTCGAGCACGTC
GGCGTCACCGCGCGCCCCAAGGTGACCGGCCGGCGCGGCATCCAGGTCTGGATCCCTGTGGTCCCCGGCTACACCTTCGA
CGACACCCGCGCGTGGGTCGAGACGCTGTCGCGGACCGTCGGGAAGGTGGTGCCCGAGCTGGTCAGCTGGAAGTGGGAGG
TCAAGCAGCGCAAGGGCCTGGCCCGGCTCGACTACACCCAGAACGCGATCAACAAGACCCTGGTCGCGCCGTACTCGCCG
CGGCCGGCGCCGGGCGCGCCGGTCTCCGTGCCGATCGAGTGGGACGAGCTCGACGACCCCGACCTGCGCCCGGACCGCTG
GGCGGTCCGCACGGTCGGGGACCGGCTCGCCGAGCGGGGCGACCGTTCCGGGCGCTCCTGGGCGCCGCCCAGGAGCTGCC
GCCGCTGA

Upstream 100 bases:

>100_bases
TCGGCTTCGTCGTGTACTTCGTCTACGGCCGTCGGCACAGCCGGGTCGGCCGCGCCGAGCGTGAGGAGCGGTCCGGCCAG
CCGCGGGACACCGAGGTGCG

Downstream 100 bases:

>100_bases
AGTGAGCAGTCAGACGACGCGGACGGGCGTGCGGCGCACGGTGAAGTCCCAGACCCGCTTCGAGAGCGCCCGGCTCAGCC
GGATGCAGCCGTGCGAGCGT

Product: DNA primase-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTGKLDAYRRKRDFTRTPEPAGAPAPGAGTGRFVVQRHRARRLHYDPRAAVEAGELHAEVHGRKLRGRLVLVRRDGGGDS
GGGDDWMLLHKHDEHAVEGWDPEDHPVSVVSGRTNDQVKADPDRLWRSDAPAAEASVSLLPEPLPDEAIDELESFGEQGT
WEVFGRRLEVTNLDKVLFPAPADGPPVSKRELLAYAARVAPVALPYLEGRALNMHRYPEGADRPGFWHKQRPEHAPEWLT
CWENPDADEGETTAYVVPDEPAALVWAANFGALEWHPWTSRTGRPHEPTYALIDLDPGERTRWADLLVLARLHRTALEHV
GVTARPKVTGRRGIQVWIPVVPGYTFDDTRAWVETLSRTVGKVVPELVSWKWEVKQRKGLARLDYTQNAINKTLVAPYSP
RPAPGAPVSVPIEWDELDDPDLRPDRWAVRTVGDRLAERGDRSGRSWAPPRSCRR

Sequences:

>Translated_455_residues
MTGKLDAYRRKRDFTRTPEPAGAPAPGAGTGRFVVQRHRARRLHYDPRAAVEAGELHAEVHGRKLRGRLVLVRRDGGGDS
GGGDDWMLLHKHDEHAVEGWDPEDHPVSVVSGRTNDQVKADPDRLWRSDAPAAEASVSLLPEPLPDEAIDELESFGEQGT
WEVFGRRLEVTNLDKVLFPAPADGPPVSKRELLAYAARVAPVALPYLEGRALNMHRYPEGADRPGFWHKQRPEHAPEWLT
CWENPDADEGETTAYVVPDEPAALVWAANFGALEWHPWTSRTGRPHEPTYALIDLDPGERTRWADLLVLARLHRTALEHV
GVTARPKVTGRRGIQVWIPVVPGYTFDDTRAWVETLSRTVGKVVPELVSWKWEVKQRKGLARLDYTQNAINKTLVAPYSP
RPAPGAPVSVPIEWDELDDPDLRPDRWAVRTVGDRLAERGDRSGRSWAPPRSCRR
>Mature_454_residues
TGKLDAYRRKRDFTRTPEPAGAPAPGAGTGRFVVQRHRARRLHYDPRAAVEAGELHAEVHGRKLRGRLVLVRRDGGGDSG
GGDDWMLLHKHDEHAVEGWDPEDHPVSVVSGRTNDQVKADPDRLWRSDAPAAEASVSLLPEPLPDEAIDELESFGEQGTW
EVFGRRLEVTNLDKVLFPAPADGPPVSKRELLAYAARVAPVALPYLEGRALNMHRYPEGADRPGFWHKQRPEHAPEWLTC
WENPDADEGETTAYVVPDEPAALVWAANFGALEWHPWTSRTGRPHEPTYALIDLDPGERTRWADLLVLARLHRTALEHVG
VTARPKVTGRRGIQVWIPVVPGYTFDDTRAWVETLSRTVGKVVPELVSWKWEVKQRKGLARLDYTQNAINKTLVAPYSPR
PAPGAPVSVPIEWDELDDPDLRPDRWAVRTVGDRLAERGDRSGRSWAPPRSCRR

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: COG3285

COG function: function code L; Predicted eukaryotic-type DNA primase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 51031; Mature: 50899

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKLDAYRRKRDFTRTPEPAGAPAPGAGTGRFVVQRHRARRLHYDPRAAVEAGELHAEV
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HGRKLRGRLVLVRRDGGGDSGGGDDWMLLHKHDEHAVEGWDPEDHPVSVVSGRTNDQVKA
CCEEECCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCC
DPDRLWRSDAPAAEASVSLLPEPLPDEAIDELESFGEQGTWEVFGRRLEVTNLDKVLFPA
CHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEECC
PADGPPVSKRELLAYAARVAPVALPYLEGRALNMHRYPEGADRPGFWHKQRPEHAPEWLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
CWENPDADEGETTAYVVPDEPAALVWAANFGALEWHPWTSRTGRPHEPTYALIDLDPGER
ECCCCCCCCCCEEEEEECCCCCEEEEEECCCCEEECCCCCCCCCCCCCCEEEEECCCCCC
TRWADLLVLARLHRTALEHVGVTARPKVTGRRGIQVWIPVVPGYTFDDTRAWVETLSRTV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
GKVVPELVSWKWEVKQRKGLARLDYTQNAINKTLVAPYSPRPAPGAPVSVPIEWDELDDP
HHHHHHHHCCEEHHHHHCCCEEEHHHHHHHCCEEECCCCCCCCCCCCEEEEEECCCCCCC
DLRPDRWAVRTVGDRLAERGDRSGRSWAPPRSCRR
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
TGKLDAYRRKRDFTRTPEPAGAPAPGAGTGRFVVQRHRARRLHYDPRAAVEAGELHAEV
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HGRKLRGRLVLVRRDGGGDSGGGDDWMLLHKHDEHAVEGWDPEDHPVSVVSGRTNDQVKA
CCEEECCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCC
DPDRLWRSDAPAAEASVSLLPEPLPDEAIDELESFGEQGTWEVFGRRLEVTNLDKVLFPA
CHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEECC
PADGPPVSKRELLAYAARVAPVALPYLEGRALNMHRYPEGADRPGFWHKQRPEHAPEWLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
CWENPDADEGETTAYVVPDEPAALVWAANFGALEWHPWTSRTGRPHEPTYALIDLDPGER
ECCCCCCCCCCEEEEEECCCCCEEEEEECCCCEEECCCCCCCCCCCCCCEEEEECCCCCC
TRWADLLVLARLHRTALEHVGVTARPKVTGRRGIQVWIPVVPGYTFDDTRAWVETLSRTV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
GKVVPELVSWKWEVKQRKGLARLDYTQNAINKTLVAPYSPRPAPGAPVSVPIEWDELDDP
HHHHHHHHCCEEHHHHHCCCEEEHHHHHHHCCEEECCCCCCCCCCCCEEEEEECCCCCCC
DLRPDRWAVRTVGDRLAERGDRSGRSWAPPRSCRR
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]